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The map label for this gene is lacZ [H]

Identifier: 220932393

GI number: 220932393

Start: 1667964

End: 1670198

Strand: Reverse

Name: lacZ [H]

Synonym: Hore_15570

Alternate gene names: 220932393

Gene position: 1670198-1667964 (Counterclockwise)

Preceding gene: 220932394

Following gene: 220932392

Centisome position: 64.78

GC content: 39.33

Gene sequence:

>2235_bases
ATGGATGGAGCAATAATTGAACTAAACGGCAACTGGCTTATCAAACATGACCCTGAAGATAAACTGGAAAAAGAGGGGAT
TATGTACCGGGACAGTATTGAGACTGCAGGATGGAATAGAATTGAGGTTCCGTCCCACTGGCAAAATCAGGGTTATGATT
ATCAGGGAGTGGTCTGGTATTTAAAAGAATTTAAAGTTGAAAATGATAGACTTGATGATAACAGGAAAGCTTTTCTGAAT
TTTAAAGGTGTTGATTATAGATGTCAGGTCTGGTTAAATGGGGTTTATTTCGGTACCCATGAAGGGGATTTTGATAGTTT
TGAATTTGAAGTATCAGATTTTTTAAAGCAAGAAAATAGATTGCTTGTAAAAGTAGAGAGCTATGTAGACAGAAGACCGG
AATTTAAAACAACCCTCAAAGGAGGGAATTACCACTGGGATTGTTTACCTGTTAAACAACAGGGATTAAAGGATTGTCCT
GAGGTTCCGTCAGCGTCCAATCCGCAGTATCCAAATCCTGTAGTAAACCCCGGTGGTATCTGGAAGCCTGTTTTTCTTGA
GATTACAGAACCTTTCCGGGTTGAAGAATTGTTTATTACCCCGGAGTTTAAGGGTTCTCAAAAAGAGGTTGAGCTATATA
TAAAGGGTAAGGTCTATAACTGGACCGATGACCCGGTCTCCTGCCAGGGAAATATTAAACTTATCCCTGATAATTTTAGA
GGAGACTCATTTGTAAAAGATATTGACCTGTATTTGACCCCCGGATATAACTATATATCCCTTAAAATGGAACTTAACAG
ACCTGAATTATGGTGGACATGGGATCTGGGAAATCCTAATTTATATAGATTTGAACTAAAACTGAATGATGAAAACGACC
GTCAGATTATAAAATATACCCGTTTAATCGGTATCAGGAAAGTTGAAAAGGGTAGTAACTGGGAGCTTTACTTAAACGGA
AAGAGATTTTTTGCCCGTGGTACCAATTATCTTTCCGACCATTACCTCTCAAGAACCGATAAAGAACGTTATGATACTGA
TATAGAGATGATGGTTGAGGCCAATATGAATATGGTCAGGGTTTTTTCCCATATGGAAAAAGATTACTTCTATGAAGAGT
GTGACCGCAGGGGAATTCTGGTTTTTCAGGATTTACCCTTTCAGTGGGGTTATGAGCCTGACCCGGTAATCATTGAACGG
GCCAGGAGCATTTCTGAAAGATTTGTTAAAAAATTGTATAACCACCCTTCAATTTTCCTGTGGTGCTGTCACAGTGAGTC
AAGGTATCATGATTACAATAAACTTGACCGGATTGTAGAAGCTACAATAAGAAAAATTGACCCCGGGCGGCCGGTCTGGA
GGGACTCAGTGATTATGACTTCTGGTGAACCTCCAGAATATTTTGGAAGCCTGGATGAATTCGAAGACTATGTCAGTGCC
AATATCAGTGTCCACTGGGTTGGCTGGTACTGGGGAGAGATAGGGGATGCCGAATATTATAATCCCCTGTTTATAACTGA
ATTTGGGACCCAGTCTTTACCAGGTATCGAATCTTTAAAAAAGTTTATACCTGAAGAGAAATTATGGCCACCTGATTGGG
ATGAATACCGTTATCGTGGTTTTCAAACCAATATCTATCAGGAAAATATGGGTAGTTTCCCCGAGAAATTGGAGGACCTG
GTCAGGATAACCCAGGATTATCAGCATAAATTTTATAAAAAGCATATTGAAGCCTTGAGGAGAAAGAAATACAGGAATGT
TAATGGATTGTTACAGTTCCACTTTGTCAATACATGGCCTGCAATTGACTGGTCAATAATAGATTATTACCGGAAACCAA
AGAAGGCGTACTTTACTGTGAAAGATGCCTTTAAACCGGTTTTAGTATCCTTTACCGCTATATTTAATCAGGGCTCTGTT
GAGGCCAGAGCCTGGGTAGTCAATGATTATCACCATTCCCTTGAAGACCTTACTTTAAGGTGGGAGATAACCGGGAAAGA
AGAACATATTAATAAAGAGGTTGAAATACCGGTTATCGAAGCGGATTTAGCTGGCATTATTGCCAGGGAAATAATTTCCG
GTGATTTTAAAGAAATCAGGGTAAGGGGTGAACTCATCGATAAGTCAGGTAAAGTTCTTTCTGCTAATAAAGATACCTTG
ATTCCCCTGAAGAAATATAAATATGTTGAAAGGGTAGACGGAGTAAAGGTTAACAGAAAAGCTGACGCCATTTAA

Upstream 100 bases:

>100_bases
ATCAATGTGCAGCTGCCGTACAGGCTTATAGAGAGGGAAAGCACCAGGAAAATAAGATGATTTTATTAACCGATATGATA
TTTTTCCAGGAGGGATTAAA

Downstream 100 bases:

>100_bases
ATTATATAGCCATATAAATCTTATAAAATGTATTTCGAGAATAGTTTCAAATAACTTATCCCTGTATTCTGGTAACCAAT
ACAGAAAAGTTCAGGTGACA

Product: glycoside hydrolase family 2 sugar binding

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MDGAIIELNGNWLIKHDPEDKLEKEGIMYRDSIETAGWNRIEVPSHWQNQGYDYQGVVWYLKEFKVENDRLDDNRKAFLN
FKGVDYRCQVWLNGVYFGTHEGDFDSFEFEVSDFLKQENRLLVKVESYVDRRPEFKTTLKGGNYHWDCLPVKQQGLKDCP
EVPSASNPQYPNPVVNPGGIWKPVFLEITEPFRVEELFITPEFKGSQKEVELYIKGKVYNWTDDPVSCQGNIKLIPDNFR
GDSFVKDIDLYLTPGYNYISLKMELNRPELWWTWDLGNPNLYRFELKLNDENDRQIIKYTRLIGIRKVEKGSNWELYLNG
KRFFARGTNYLSDHYLSRTDKERYDTDIEMMVEANMNMVRVFSHMEKDYFYEECDRRGILVFQDLPFQWGYEPDPVIIER
ARSISERFVKKLYNHPSIFLWCCHSESRYHDYNKLDRIVEATIRKIDPGRPVWRDSVIMTSGEPPEYFGSLDEFEDYVSA
NISVHWVGWYWGEIGDAEYYNPLFITEFGTQSLPGIESLKKFIPEEKLWPPDWDEYRYRGFQTNIYQENMGSFPEKLEDL
VRITQDYQHKFYKKHIEALRRKKYRNVNGLLQFHFVNTWPAIDWSIIDYYRKPKKAYFTVKDAFKPVLVSFTAIFNQGSV
EARAWVVNDYHHSLEDLTLRWEITGKEEHINKEVEIPVIEADLAGIIAREIISGDFKEIRVRGELIDKSGKVLSANKDTL
IPLKKYKYVERVDGVKVNRKADAI

Sequences:

>Translated_744_residues
MDGAIIELNGNWLIKHDPEDKLEKEGIMYRDSIETAGWNRIEVPSHWQNQGYDYQGVVWYLKEFKVENDRLDDNRKAFLN
FKGVDYRCQVWLNGVYFGTHEGDFDSFEFEVSDFLKQENRLLVKVESYVDRRPEFKTTLKGGNYHWDCLPVKQQGLKDCP
EVPSASNPQYPNPVVNPGGIWKPVFLEITEPFRVEELFITPEFKGSQKEVELYIKGKVYNWTDDPVSCQGNIKLIPDNFR
GDSFVKDIDLYLTPGYNYISLKMELNRPELWWTWDLGNPNLYRFELKLNDENDRQIIKYTRLIGIRKVEKGSNWELYLNG
KRFFARGTNYLSDHYLSRTDKERYDTDIEMMVEANMNMVRVFSHMEKDYFYEECDRRGILVFQDLPFQWGYEPDPVIIER
ARSISERFVKKLYNHPSIFLWCCHSESRYHDYNKLDRIVEATIRKIDPGRPVWRDSVIMTSGEPPEYFGSLDEFEDYVSA
NISVHWVGWYWGEIGDAEYYNPLFITEFGTQSLPGIESLKKFIPEEKLWPPDWDEYRYRGFQTNIYQENMGSFPEKLEDL
VRITQDYQHKFYKKHIEALRRKKYRNVNGLLQFHFVNTWPAIDWSIIDYYRKPKKAYFTVKDAFKPVLVSFTAIFNQGSV
EARAWVVNDYHHSLEDLTLRWEITGKEEHINKEVEIPVIEADLAGIIAREIISGDFKEIRVRGELIDKSGKVLSANKDTL
IPLKKYKYVERVDGVKVNRKADAI
>Mature_744_residues
MDGAIIELNGNWLIKHDPEDKLEKEGIMYRDSIETAGWNRIEVPSHWQNQGYDYQGVVWYLKEFKVENDRLDDNRKAFLN
FKGVDYRCQVWLNGVYFGTHEGDFDSFEFEVSDFLKQENRLLVKVESYVDRRPEFKTTLKGGNYHWDCLPVKQQGLKDCP
EVPSASNPQYPNPVVNPGGIWKPVFLEITEPFRVEELFITPEFKGSQKEVELYIKGKVYNWTDDPVSCQGNIKLIPDNFR
GDSFVKDIDLYLTPGYNYISLKMELNRPELWWTWDLGNPNLYRFELKLNDENDRQIIKYTRLIGIRKVEKGSNWELYLNG
KRFFARGTNYLSDHYLSRTDKERYDTDIEMMVEANMNMVRVFSHMEKDYFYEECDRRGILVFQDLPFQWGYEPDPVIIER
ARSISERFVKKLYNHPSIFLWCCHSESRYHDYNKLDRIVEATIRKIDPGRPVWRDSVIMTSGEPPEYFGSLDEFEDYVSA
NISVHWVGWYWGEIGDAEYYNPLFITEFGTQSLPGIESLKKFIPEEKLWPPDWDEYRYRGFQTNIYQENMGSFPEKLEDL
VRITQDYQHKFYKKHIEALRRKKYRNVNGLLQFHFVNTWPAIDWSIIDYYRKPKKAYFTVKDAFKPVLVSFTAIFNQGSV
EARAWVVNDYHHSLEDLTLRWEITGKEEHINKEVEIPVIEADLAGIIAREIISGDFKEIRVRGELIDKSGKVLSANKDTL
IPLKKYKYVERVDGVKVNRKADAI

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI84798622, Length=646, Percent_Identity=24.4582043343653, Blast_Score=119, Evalue=2e-26,
Organism=Escherichia coli, GI1786539, Length=483, Percent_Identity=24.6376811594203, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17550784, Length=754, Percent_Identity=21.6180371352785, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24643838, Length=644, Percent_Identity=22.0496894409938, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24655438, Length=537, Percent_Identity=23.0912476722533, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922582, Length=537, Percent_Identity=23.0912476722533, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI62471735, Length=537, Percent_Identity=23.0912476722533, Blast_Score=94, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24643840, Length=619, Percent_Identity=21.8093699515347, Blast_Score=84, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 88163; Mature: 88163

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGAIIELNGNWLIKHDPEDKLEKEGIMYRDSIETAGWNRIEVPSHWQNQGYDYQGVVWY
CCCEEEEECCCEEEEECCHHHHHHCCCEEEECCCCCCCCEEECCCCHHCCCCCCCHHEEE
LKEFKVENDRLDDNRKAFLNFKGVDYRCQVWLNGVYFGTHEGDFDSFEFEVSDFLKQENR
EEHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCEEEHHHHHHHCCCC
LLVKVESYVDRRPEFKTTLKGGNYHWDCLPVKQQGLKDCPEVPSASNPQYPNPVVNPGGI
EEEEEEHHHCCCCCCEEEECCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
WKPVFLEITEPFRVEELFITPEFKGSQKEVELYIKGKVYNWTDDPVSCQGNIKLIPDNFR
CCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCEECCCCEEEECCCCC
GDSFVKDIDLYLTPGYNYISLKMELNRPELWWTWDLGNPNLYRFELKLNDENDRQIIKYT
CCCEEEEEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHH
RLIGIRKVEKGSNWELYLNGKRFFARGTNYLSDHYLSRTDKERYDTDIEMMVEANMNMVR
HHHHEEEECCCCCEEEEECCCEEEECCCCHHHHHHHCCCCHHHCCCCEEEEEECCCCHHH
VFSHMEKDYFYEECDRRGILVFQDLPFQWGYEPDPVIIERARSISERFVKKLYNHPSIFL
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
WCCHSESRYHDYNKLDRIVEATIRKIDPGRPVWRDSVIMTSGEPPEYFGSLDEFEDYVSA
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHCCHHHHHHHHHC
NISVHWVGWYWGEIGDAEYYNPLFITEFGTQSLPGIESLKKFIPEEKLWPPDWDEYRYRG
CEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCC
FQTNIYQENMGSFPEKLEDLVRITQDYQHKFYKKHIEALRRKKYRNVNGLLQFHFVNTWP
CCCCEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
AIDWSIIDYYRKPKKAYFTVKDAFKPVLVSFTAIFNQGSVEARAWVVNDYHHSLEDLTLR
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHCCCCCEEEE
WEITGKEEHINKEVEIPVIEADLAGIIAREIISGDFKEIRVRGELIDKSGKVLSANKDTL
EEECCCHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCEEECCCCCE
IPLKKYKYVERVDGVKVNRKADAI
EEHHHHHHHHHHCCEEECCCCCCC
>Mature Secondary Structure
MDGAIIELNGNWLIKHDPEDKLEKEGIMYRDSIETAGWNRIEVPSHWQNQGYDYQGVVWY
CCCEEEEECCCEEEEECCHHHHHHCCCEEEECCCCCCCCEEECCCCHHCCCCCCCHHEEE
LKEFKVENDRLDDNRKAFLNFKGVDYRCQVWLNGVYFGTHEGDFDSFEFEVSDFLKQENR
EEHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCEEEHHHHHHHCCCC
LLVKVESYVDRRPEFKTTLKGGNYHWDCLPVKQQGLKDCPEVPSASNPQYPNPVVNPGGI
EEEEEEHHHCCCCCCEEEECCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
WKPVFLEITEPFRVEELFITPEFKGSQKEVELYIKGKVYNWTDDPVSCQGNIKLIPDNFR
CCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCEECCCCEEEECCCCC
GDSFVKDIDLYLTPGYNYISLKMELNRPELWWTWDLGNPNLYRFELKLNDENDRQIIKYT
CCCEEEEEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHH
RLIGIRKVEKGSNWELYLNGKRFFARGTNYLSDHYLSRTDKERYDTDIEMMVEANMNMVR
HHHHEEEECCCCCEEEEECCCEEEECCCCHHHHHHHCCCCHHHCCCCEEEEEECCCCHHH
VFSHMEKDYFYEECDRRGILVFQDLPFQWGYEPDPVIIERARSISERFVKKLYNHPSIFL
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
WCCHSESRYHDYNKLDRIVEATIRKIDPGRPVWRDSVIMTSGEPPEYFGSLDEFEDYVSA
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHCCHHHHHHHHHC
NISVHWVGWYWGEIGDAEYYNPLFITEFGTQSLPGIESLKKFIPEEKLWPPDWDEYRYRG
CEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCC
FQTNIYQENMGSFPEKLEDLVRITQDYQHKFYKKHIEALRRKKYRNVNGLLQFHFVNTWP
CCCCEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
AIDWSIIDYYRKPKKAYFTVKDAFKPVLVSFTAIFNQGSVEARAWVVNDYHHSLEDLTLR
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHCCCCCEEEE
WEITGKEEHINKEVEIPVIEADLAGIIAREIISGDFKEIRVRGELIDKSGKVLSANKDTL
EEECCCHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCEEECCCCCE
IPLKKYKYVERVDGVKVNRKADAI
EEHHHHHHHHHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA