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The map label for this gene is yabR [H]

Identifier: 220932348

GI number: 220932348

Start: 1613638

End: 1614768

Strand: Reverse

Name: yabR [H]

Synonym: Hore_15110

Alternate gene names: 220932348

Gene position: 1614768-1613638 (Counterclockwise)

Preceding gene: 220932349

Following gene: 220932347

Centisome position: 62.63

GC content: 32.36

Gene sequence:

>1131_bases
ATGAAGGAAAGGGGGAGAAGAAAGATCAGCAGGCATAATTTTAAAGAAGGGGATATTGTTGAAGGAAGAGTAACAAGGAT
AAAACCTTATGGTGCCTTTGTGCGACTTGATGAAAAAAAGAAAATTGATGGGTTGATTTATATATCAAATATTGCTGATA
AATATGTTAAGGATATAACAAATTATATCAACGAAGGGGATCAAATTAGAGTAAAGATAATTAATATTGAAAAAGATGGG
AAAATAGCTCTTAGTTTCAAATATAAATCTTACATACCAAATGATACTAATAAAATTATTAAAAAGACTGCTATTGAAAA
CTTCTACCTTCAGACTAATTATTATATATATGACTTCGATGAAAGTGATAAAAAAGACATACTTGCAAACCTAACTAAAA
ATCAAAATCAGTTTTTTTCAACTAAAGAAATAAAAAGCTTCTTAAGCAGGCAGGAAAAACAAATCAGCTATTTGTCCTCA
ATAGGATATAGTGTTGATAATTTTGTAATGACTAATGATTATAGAATGGTTGTTGGTTTAGGGGGAGCAAATGTACTGGA
AACCAATTTAATGTTACATCATATTTATGGAATACCATATATACCTGGAAGTTCATTGAAAGGACTGACCAGGGCCTGGG
CTGTTGAAAAATTGAGGGAAGAATTAGATGTTAAAAGTTATGAAAAAATAGAATCTTTACTCGGAGAGGAAAAAATAGAT
GTAAAAACACTGGATGAATACCAGATGAATCAATTTGAAAAATATCGCTGGATTTTTGGAACGAAAAGTCAGAGTGGTAA
TGCGGTCTTTTTTGATGCTTATCCGGAGGGCAGAATAAATATAGATATCGATATCATGACTCCTCATTTTAAAGAATATT
ATCAGGAAATGGAAACGACGAAGAAAGTCAATAATCCTCCTGATGACACTCAAAGTCCAGTACCTATTCCCTTCCTGGTA
TTGGATAATCAAATGTTCAGGTTTAGTGTTGCTGTTAAATGTAAGGAGTGTGCGGGAAAAAGAGATAACAGTTGTGAGTA
TTTGAACCTGACTGTTAATTTGCTCAAAGAAGCTTTGAGAAATTTAGGTGTTGGTGCTAAAACCATGGTTGGCTACGGGT
ATTTTGAGTAA

Upstream 100 bases:

>100_bases
GAAGTTGTTTCAACTGATAGCAAAAATTATAGGCAGTTAACTAAAGAAACCCTTGCTTTATTAAACTGGTGGAGAAGATT
TGTTGATGGAATGATTGAAG

Downstream 100 bases:

>100_bases
GGAGATGAAATATATTTATGAAAAAAATATTAACTACTGTGGGTACATCATTATTAACACATTTAAGGGAAGAAGAAGAG
ATAGGCAAAAGTGAATATGA

Product: CRISPR-associated RAMP protein, Cmr6 family

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKERGRRKISRHNFKEGDIVEGRVTRIKPYGAFVRLDEKKKIDGLIYISNIADKYVKDITNYINEGDQIRVKIINIEKDG
KIALSFKYKSYIPNDTNKIIKKTAIENFYLQTNYYIYDFDESDKKDILANLTKNQNQFFSTKEIKSFLSRQEKQISYLSS
IGYSVDNFVMTNDYRMVVGLGGANVLETNLMLHHIYGIPYIPGSSLKGLTRAWAVEKLREELDVKSYEKIESLLGEEKID
VKTLDEYQMNQFEKYRWIFGTKSQSGNAVFFDAYPEGRINIDIDIMTPHFKEYYQEMETTKKVNNPPDDTQSPVPIPFLV
LDNQMFRFSVAVKCKECAGKRDNSCEYLNLTVNLLKEALRNLGVGAKTMVGYGYFE

Sequences:

>Translated_376_residues
MKERGRRKISRHNFKEGDIVEGRVTRIKPYGAFVRLDEKKKIDGLIYISNIADKYVKDITNYINEGDQIRVKIINIEKDG
KIALSFKYKSYIPNDTNKIIKKTAIENFYLQTNYYIYDFDESDKKDILANLTKNQNQFFSTKEIKSFLSRQEKQISYLSS
IGYSVDNFVMTNDYRMVVGLGGANVLETNLMLHHIYGIPYIPGSSLKGLTRAWAVEKLREELDVKSYEKIESLLGEEKID
VKTLDEYQMNQFEKYRWIFGTKSQSGNAVFFDAYPEGRINIDIDIMTPHFKEYYQEMETTKKVNNPPDDTQSPVPIPFLV
LDNQMFRFSVAVKCKECAGKRDNSCEYLNLTVNLLKEALRNLGVGAKTMVGYGYFE
>Mature_376_residues
MKERGRRKISRHNFKEGDIVEGRVTRIKPYGAFVRLDEKKKIDGLIYISNIADKYVKDITNYINEGDQIRVKIINIEKDG
KIALSFKYKSYIPNDTNKIIKKTAIENFYLQTNYYIYDFDESDKKDILANLTKNQNQFFSTKEIKSFLSRQEKQISYLSS
IGYSVDNFVMTNDYRMVVGLGGANVLETNLMLHHIYGIPYIPGSSLKGLTRAWAVEKLREELDVKSYEKIESLLGEEKID
VKTLDEYQMNQFEKYRWIFGTKSQSGNAVFFDAYPEGRINIDIDIMTPHFKEYYQEMETTKKVNNPPDDTQSPVPIPFLV
LDNQMFRFSVAVKCKECAGKRDNSCEYLNLTVNLLKEALRNLGVGAKTMVGYGYFE

Specific function: Involved In Mrna Degradation. Hydrolyzes Single-Stranded Polyribonucleotides Processively In The 3' To 5' Direction. Involved In The RNA Degradosome, A Multi-Enzyme Complex Important In RNA Processing And Messenger RNA Degradation. [C]

COG id: COG1604

COG function: function code L; Uncharacterized protein predicted to be involved in DNA repair (RAMP superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI145693187, Length=71, Percent_Identity=42.2535211267606, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 43638; Mature: 43638

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERGRRKISRHNFKEGDIVEGRVTRIKPYGAFVRLDEKKKIDGLIYISNIADKYVKDIT
CCCHHHHHHHHCCCCCCCEECCEEEEEECCCEEEEECCCCCCCCEEEEHHHHHHHHHHHH
NYINEGDQIRVKIINIEKDGKIALSFKYKSYIPNDTNKIIKKTAIENFYLQTNYYIYDFD
HHHCCCCEEEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCEEEEEEEEEEEECC
ESDKKDILANLTKNQNQFFSTKEIKSFLSRQEKQISYLSSIGYSVDNFVMTNDYRMVVGL
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEC
GGANVLETNLMLHHIYGIPYIPGSSLKGLTRAWAVEKLREELDVKSYEKIESLLGEEKID
CCCCHHHHHHHEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
VKTLDEYQMNQFEKYRWIFGTKSQSGNAVFFDAYPEGRINIDIDIMTPHFKEYYQEMETT
EEECCHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCEEEEEEEEECHHHHHHHHHHHHH
KKVNNPPDDTQSPVPIPFLVLDNQMFRFSVAVKCKECAGKRDNSCEYLNLTVNLLKEALR
HHCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEEHHHCCCCCCCCEEEEHHHHHHHHHHH
NLGVGAKTMVGYGYFE
HCCCCCHHHCCCCCCC
>Mature Secondary Structure
MKERGRRKISRHNFKEGDIVEGRVTRIKPYGAFVRLDEKKKIDGLIYISNIADKYVKDIT
CCCHHHHHHHHCCCCCCCEECCEEEEEECCCEEEEECCCCCCCCEEEEHHHHHHHHHHHH
NYINEGDQIRVKIINIEKDGKIALSFKYKSYIPNDTNKIIKKTAIENFYLQTNYYIYDFD
HHHCCCCEEEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCEEEEEEEEEEEECC
ESDKKDILANLTKNQNQFFSTKEIKSFLSRQEKQISYLSSIGYSVDNFVMTNDYRMVVGL
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEC
GGANVLETNLMLHHIYGIPYIPGSSLKGLTRAWAVEKLREELDVKSYEKIESLLGEEKID
CCCCHHHHHHHEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
VKTLDEYQMNQFEKYRWIFGTKSQSGNAVFFDAYPEGRINIDIDIMTPHFKEYYQEMETT
EEECCHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCEEEEEEEEECHHHHHHHHHHHHH
KKVNNPPDDTQSPVPIPFLVLDNQMFRFSVAVKCKECAGKRDNSCEYLNLTVNLLKEALR
HHCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEEHHHCCCCCCCCEEEEHHHHHHHHHHH
NLGVGAKTMVGYGYFE
HCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113187; 7584024; 9384377 [H]