Click here to switch to the map view.

The map label for this gene is ptsH [H]

Identifier: 220932285

GI number: 220932285

Start: 1539357

End: 1539623

Strand: Reverse

Name: ptsH [H]

Synonym: Hore_14490

Alternate gene names: 220932285

Gene position: 1539623-1539357 (Counterclockwise)

Preceding gene: 220932286

Following gene: 220932281

Centisome position: 59.72

GC content: 38.2

Gene sequence:

>267_bases
ATGATTACAGAGCATATTAAGGTAAAAAATGAAGAGGGATTACATGCCAGGCCTGCATCAAAATTTGTTAAAAAGGCAAA
TAGTTATCGCAGTAATATTGAAATTATTTTTAAGGACCTCAGGGCTGATGGAAAAAGTATTATGGGGGTAATGAGTCTGG
GAATCAGGAGGGGTGACAAAATTACCCTGAAAATAGACGGGCCTGATGAAGAAAAGGCCCGTCAGGAACTAATTGATTTC
TTTCAAGCCGGTCTTGTAAAAAGTTGA

Upstream 100 bases:

>100_bases
ACTATAGATAGTATTAGAAGTTTATCGAAAAATATAGTTTAGAACATAAGATTAATATAAACTTAATATAAACTTAATAT
AAAAAGGAAGGTTTAAGTTA

Downstream 100 bases:

>100_bases
TTTTTTATCCACCCAGGGTTATATCCTGCTTATCATACCATGCAAGGGCTTCATAAAAATGTTCAGGTTTAAAATCGGGC
CAGTAATCGTCAATGACATA

Product: Phosphotransferase system, phosphocarrier protein HPr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 88; Mature: 88

Protein sequence:

>88_residues
MITEHIKVKNEEGLHARPASKFVKKANSYRSNIEIIFKDLRADGKSIMGVMSLGIRRGDKITLKIDGPDEEKARQELIDF
FQAGLVKS

Sequences:

>Translated_88_residues
MITEHIKVKNEEGLHARPASKFVKKANSYRSNIEIIFKDLRADGKSIMGVMSLGIRRGDKITLKIDGPDEEKARQELIDF
FQAGLVKS
>Mature_88_residues
MITEHIKVKNEEGLHARPASKFVKKANSYRSNIEIIFKDLRADGKSIMGVMSLGIRRGDKITLKIDGPDEEKARQELIDF
FQAGLVKS

Specific function: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbo

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788755, Length=78, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 9930; Mature: 9930

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITEHIKVKNEEGLHARPASKFVKKANSYRSNIEIIFKDLRADGKSIMGVMSLGIRRGDK
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCE
ITLKIDGPDEEKARQELIDFFQAGLVKS
EEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITEHIKVKNEEGLHARPASKFVKKANSYRSNIEIIFKDLRADGKSIMGVMSLGIRRGDK
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCE
ITLKIDGPDEEKARQELIDFFQAGLVKS
EEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]