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The map label for this gene is ptsI [H]

Identifier: 220932281

GI number: 220932281

Start: 1535521

End: 1537239

Strand: Reverse

Name: ptsI [H]

Synonym: Hore_14450

Alternate gene names: 220932281

Gene position: 1537239-1535521 (Counterclockwise)

Preceding gene: 220932285

Following gene: 220932280

Centisome position: 59.63

GC content: 40.26

Gene sequence:

>1719_bases
ATGAATGGAATAGGGGCTTCACCAGGAATAGCAATAGGCAAGGTTTATATAAAAAACAGTAGTGGGTTTAATATTACTGA
AAAAAATATAGATGAACATGAAGTACAGGGGGAAATTGAAAGGCTTAATGATGCCCTGGAAAAAGCAAAAAAGGAAGTAA
CTAAACTGGAACATAAGGTTAGAAAGGAACTCGGAGAGGATGAAGCCGATATATTCCGGGCCCATCTACAACTTCTTAAT
GACCCTGAACTTATACCTGAAATAGAAAATGAGATTAAAAACAACCTGGTTACGGCTGAAACTGCAGTACAAGAAGTTAT
CAACCATTATGTAAGACTCTTCTCCAGAATGGAGAATGAGTATATGAAGGGGCGAAAGCTTGATATCCAGGATGTCGGGA
CCAGGGTCCTCCAAATCCTGGTAAATTACAAAAAAGATCATCTTAATAAGGATATTGGTAATGAATCAATAATTGTGGCC
CGTGATCTGGCCCCGTCGGATACTGTTAATATTAATCTGGACAGGGTTATGGCCTTTGTTACAGCTGAAGGCTCAAAGAC
CTCCCATACAGCTATAATTGCCCGGTCCCTGGGGATACCTGCAGTTGTAGGGGTTGGAGAGGAGCTTCTTAAAAAAGTAC
ATAATGGTCAAATGATAGTTGTTGATGGAAATTCCGGTAAGGTTTACCTTAACCCCGATAAGGCTACTCTAGATAAATAT
AAAATGAAGTTAAACAGGTTAGCCAGAGATAAAAAACGGCTAAATAAATATAAATTTAAAAAGCCGGTTACCAGGGATGG
GGTTACTGTAGAGGTTTTTGCCAACATTAGTTCTCCGGAAGAGGTCGGGGCAGTTATTGATAATGGTGGTCAGGGAGTAG
GGTTATTTAGAACTGAGTTTATGTATATGCACAGGGATAGTCTCCCTGATGAAGAAGAACAATACAACCTGTATTTAGAG
ATAGCTAAAAAAATGGACAGGAAACCGGTTATTATCAGGACCCTGGATATCGGTGGAGATAAAGACCTACCTTATCTTAA
TCTCCCTGAGGAGATGAACCCTTTCCTCGGTTACCGGGGAATCAGGATCTGTCTTGATAAAAAGGATTTATTTAAAACCC
AGCTTAAGGCGATATTGAGGGCTGGTAAGCATGGTAATGTAAGGTTAATGTTTCCTTTGATATCATCCCTTGAAGAATTA
AAAAAGGCCTATCATGTTTTAGAAGAGTCGAAGCAGGAACTGGAAGCTGAAGGTAAGGAATATGTTAAAGATATGGAAAC
AGGTCTCATGATTGAAGTGCCAGCAGCAGCCCTGATGGCTGATATTCTGGCCCAGGAGGTTGATTTTTTTAGTATAGGAA
CCAATGACCTGATTCAGTATATGATGGCGGTAGACAGGACAAATGAACAGGTAGCTTCTATTTATAGTCCCTACTATCCA
GCTGTTCTCAGAATGATTTATAATGTTATTAAAGCAGCCCACCGGGAATCGATCAAGGTGGGGATGTGTGGCGAGGCGGC
AGGAGATGAACTACTCATTCCGTTTTTACTGGGGGCAGGATTAGATGAATTCAGTGTCAATATCCCTGCAATTTTAAATA
TTAAAGAAAAAATAGCCAGGTGGACTATTTCTGAAGCCCGTGAAGTTGTCAAAAATACCCTGAAACTGAAAACGGCAGGC
GAGGTTAAAGATTATCTGTCGGAAGTAAAAAAGGACTAA

Upstream 100 bases:

>100_bases
TGGAAATTAAATCAGATAAGAAAACTCCCTTAAATTTAATTGTTTGGTTACTTAAATTATATTTGGATGAGTGCTGGTTT
TAAAGGGGTGATATGATAAT

Downstream 100 bases:

>100_bases
AGGAGGATATATTAAAATGCAGGCCATTATTACCGTTGTTGGAAAGGATAAGATCGGGATTATTGCCGGGGTCAGCCAGG
TATTGGCCGATTGTGGGGTT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MNGIGASPGIAIGKVYIKNSSGFNITEKNIDEHEVQGEIERLNDALEKAKKEVTKLEHKVRKELGEDEADIFRAHLQLLN
DPELIPEIENEIKNNLVTAETAVQEVINHYVRLFSRMENEYMKGRKLDIQDVGTRVLQILVNYKKDHLNKDIGNESIIVA
RDLAPSDTVNINLDRVMAFVTAEGSKTSHTAIIARSLGIPAVVGVGEELLKKVHNGQMIVVDGNSGKVYLNPDKATLDKY
KMKLNRLARDKKRLNKYKFKKPVTRDGVTVEVFANISSPEEVGAVIDNGGQGVGLFRTEFMYMHRDSLPDEEEQYNLYLE
IAKKMDRKPVIIRTLDIGGDKDLPYLNLPEEMNPFLGYRGIRICLDKKDLFKTQLKAILRAGKHGNVRLMFPLISSLEEL
KKAYHVLEESKQELEAEGKEYVKDMETGLMIEVPAAALMADILAQEVDFFSIGTNDLIQYMMAVDRTNEQVASIYSPYYP
AVLRMIYNVIKAAHRESIKVGMCGEAAGDELLIPFLLGAGLDEFSVNIPAILNIKEKIARWTISEAREVVKNTLKLKTAG
EVKDYLSEVKKD

Sequences:

>Translated_572_residues
MNGIGASPGIAIGKVYIKNSSGFNITEKNIDEHEVQGEIERLNDALEKAKKEVTKLEHKVRKELGEDEADIFRAHLQLLN
DPELIPEIENEIKNNLVTAETAVQEVINHYVRLFSRMENEYMKGRKLDIQDVGTRVLQILVNYKKDHLNKDIGNESIIVA
RDLAPSDTVNINLDRVMAFVTAEGSKTSHTAIIARSLGIPAVVGVGEELLKKVHNGQMIVVDGNSGKVYLNPDKATLDKY
KMKLNRLARDKKRLNKYKFKKPVTRDGVTVEVFANISSPEEVGAVIDNGGQGVGLFRTEFMYMHRDSLPDEEEQYNLYLE
IAKKMDRKPVIIRTLDIGGDKDLPYLNLPEEMNPFLGYRGIRICLDKKDLFKTQLKAILRAGKHGNVRLMFPLISSLEEL
KKAYHVLEESKQELEAEGKEYVKDMETGLMIEVPAAALMADILAQEVDFFSIGTNDLIQYMMAVDRTNEQVASIYSPYYP
AVLRMIYNVIKAAHRESIKVGMCGEAAGDELLIPFLLGAGLDEFSVNIPAILNIKEKIARWTISEAREVVKNTLKLKTAG
EVKDYLSEVKKD
>Mature_572_residues
MNGIGASPGIAIGKVYIKNSSGFNITEKNIDEHEVQGEIERLNDALEKAKKEVTKLEHKVRKELGEDEADIFRAHLQLLN
DPELIPEIENEIKNNLVTAETAVQEVINHYVRLFSRMENEYMKGRKLDIQDVGTRVLQILVNYKKDHLNKDIGNESIIVA
RDLAPSDTVNINLDRVMAFVTAEGSKTSHTAIIARSLGIPAVVGVGEELLKKVHNGQMIVVDGNSGKVYLNPDKATLDKY
KMKLNRLARDKKRLNKYKFKKPVTRDGVTVEVFANISSPEEVGAVIDNGGQGVGLFRTEFMYMHRDSLPDEEEQYNLYLE
IAKKMDRKPVIIRTLDIGGDKDLPYLNLPEEMNPFLGYRGIRICLDKKDLFKTQLKAILRAGKHGNVRLMFPLISSLEEL
KKAYHVLEESKQELEAEGKEYVKDMETGLMIEVPAAALMADILAQEVDFFSIGTNDLIQYMMAVDRTNEQVASIYSPYYP
AVLRMIYNVIKAAHRESIKVGMCGEAAGDELLIPFLLGAGLDEFSVNIPAILNIKEKIARWTISEAREVVKNTLKLKTAG
EVKDYLSEVKKD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=562, Percent_Identity=43.2384341637011, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=36.4541832669323, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788726, Length=534, Percent_Identity=34.0823970037453, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1789193, Length=579, Percent_Identity=32.9879101899827, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1787994, Length=439, Percent_Identity=28.0182232346241, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI226510935, Length=175, Percent_Identity=29.7142857142857, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64364; Mature: 64364

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIGASPGIAIGKVYIKNSSGFNITEKNIDEHEVQGEIERLNDALEKAKKEVTKLEHKV
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKELGEDEADIFRAHLQLLNDPELIPEIENEIKNNLVTAETAVQEVINHYVRLFSRMENE
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHH
YMKGRKLDIQDVGTRVLQILVNYKKDHLNKDIGNESIIVARDLAPSDTVNINLDRVMAFV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEHHHHEEEE
TAEGSKTSHTAIIARSLGIPAVVGVGEELLKKVHNGQMIVVDGNSGKVYLNPDKATLDKY
EECCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHH
KMKLNRLARDKKRLNKYKFKKPVTRDGVTVEVFANISSPEEVGAVIDNGGQGVGLFRTEF
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEHHHHHH
MYMHRDSLPDEEEQYNLYLEIAKKMDRKPVIIRTLDIGGDKDLPYLNLPEEMNPFLGYRG
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHCCHHHCCCC
IRICLDKKDLFKTQLKAILRAGKHGNVRLMFPLISSLEELKKAYHVLEESKQELEAEGKE
EEEEECHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVKDMETGLMIEVPAAALMADILAQEVDFFSIGTNDLIQYMMAVDRTNEQVASIYSPYYP
HHHHHHCCEEEECCHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCHHH
AVLRMIYNVIKAAHRESIKVGMCGEAAGDELLIPFLLGAGLDEFSVNIPAILNIKEKIAR
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHHCCCCCHHCCCCCCEECHHHHHHH
WTISEAREVVKNTLKLKTAGEVKDYLSEVKKD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGIGASPGIAIGKVYIKNSSGFNITEKNIDEHEVQGEIERLNDALEKAKKEVTKLEHKV
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKELGEDEADIFRAHLQLLNDPELIPEIENEIKNNLVTAETAVQEVINHYVRLFSRMENE
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHH
YMKGRKLDIQDVGTRVLQILVNYKKDHLNKDIGNESIIVARDLAPSDTVNINLDRVMAFV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEHHHHEEEE
TAEGSKTSHTAIIARSLGIPAVVGVGEELLKKVHNGQMIVVDGNSGKVYLNPDKATLDKY
EECCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHH
KMKLNRLARDKKRLNKYKFKKPVTRDGVTVEVFANISSPEEVGAVIDNGGQGVGLFRTEF
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEHHHHHH
MYMHRDSLPDEEEQYNLYLEIAKKMDRKPVIIRTLDIGGDKDLPYLNLPEEMNPFLGYRG
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHCCHHHCCCC
IRICLDKKDLFKTQLKAILRAGKHGNVRLMFPLISSLEELKKAYHVLEESKQELEAEGKE
EEEEECHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVKDMETGLMIEVPAAALMADILAQEVDFFSIGTNDLIQYMMAVDRTNEQVASIYSPYYP
HHHHHHCCEEEECCHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCHHH
AVLRMIYNVIKAAHRESIKVGMCGEAAGDELLIPFLLGAGLDEFSVNIPAILNIKEKIAR
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHHCCCCCHHCCCCCCEECHHHHHHH
WTISEAREVVKNTLKLKTAGEVKDYLSEVKKD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]