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The map label for this gene is purR [H]

Identifier: 220932158

GI number: 220932158

Start: 1420291

End: 1421328

Strand: Reverse

Name: purR [H]

Synonym: Hore_13210

Alternate gene names: 220932158

Gene position: 1421328-1420291 (Counterclockwise)

Preceding gene: 220932159

Following gene: 220932157

Centisome position: 55.13

GC content: 33.53

Gene sequence:

>1038_bases
GTGGCTGATAAACAAAAACACCATACTCTGGAAAGTATAGCTAAACTGGCCGGGGTTTCCAGGTCAACCGTTTCCAGGGT
AATTAATAATAAGGGAAATGTCAGTGAAAAAGCCCGGAAAAAGGTGCTATCAGTTATTAAAAAAGAAAAATATTATCCCA
ATGCTTCAGCCAGAAGCCTGGCTTCCCGGAAAACTAACAATATAGGGATAATATTCTGGGGTCATGACCCTGAATTTTTA
TCTAATCCAATTTATTATGAAATTCTACAGGGTATTCAAAGAGAGTCCTTAAAACATGACCTCAACCTGGTTTTGTATAC
AACCCATAAAGAAGAAGAGAAAAGACAGGAGCTTTGCTATAAGATTATTGGACAGAAAAATGTTGATGGACTAATTGTTA
TTGGTTCCCTCATGTATTTTGATTACTTAAAACTTTTTGCCAATAATGACCTTCCTGTTGTTATTATTGGGAAAAGAGAT
ACCAAAAACCTGGACATTCCATATGTTTGTACGGATTACGAAGATGGAATTTATAAAGCAACAAGGCATCTTTTAAGCCT
GGGAAGGAAAGAAATTGTTCTTTTACAGTCAACGGTGGATTATTATTTTCTTGAAGATAAATTGAGGGGTTATAAAAGAG
CACTAGAAGAAAAAAATATTAAATTTAATTCTGATTACATAATAGAAGTAACATCAAATAACGAAGATTATGTTGAAAAA
ATAGTCAGGAACCATATATTAACTATAAAAAATATTAATGCCATAATTTGTGCTAACGATATTTTAGCTCAAAATTTAAT
TAAGGTAATAACATCACAGGGAATTGCTGTACCTGACAACATCTCAGTAATTGGTTTTAATGATATAAATACAGCTAGAT
ATTTTAACCCACCATTGACAACAGTAAAACAAAATAAAATAAAGCTGGGGATAAATGCCGTCAGGACCTTATTAAGAAAA
ATAAATGGAGAAGAGACCGGTAATGTTGTTCTCGATGCTGAACTGGTGGTGAGGGGGTCAACTGCCCCTTTAAAATAA

Upstream 100 bases:

>100_bases
ATTAGTCTTGACATTTTTTTATTAAAAAATTATAATTAAAATGTAGTCATTAATTTTTTTATTCTATACTGGGAGCGCTC
CCAGTAGGAGATGGTATTTT

Downstream 100 bases:

>100_bases
ATATATTTATATATGGGAGCGCTCCCATACATGATAAAACTATAAAATTGAAATAGCTGGTTTTATCATCTAATTTGTTA
AACTTATTTTAATAAAATCT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MADKQKHHTLESIAKLAGVSRSTVSRVINNKGNVSEKARKKVLSVIKKEKYYPNASARSLASRKTNNIGIIFWGHDPEFL
SNPIYYEILQGIQRESLKHDLNLVLYTTHKEEEKRQELCYKIIGQKNVDGLIVIGSLMYFDYLKLFANNDLPVVIIGKRD
TKNLDIPYVCTDYEDGIYKATRHLLSLGRKEIVLLQSTVDYYFLEDKLRGYKRALEEKNIKFNSDYIIEVTSNNEDYVEK
IVRNHILTIKNINAIICANDILAQNLIKVITSQGIAVPDNISVIGFNDINTARYFNPPLTTVKQNKIKLGINAVRTLLRK
INGEETGNVVLDAELVVRGSTAPLK

Sequences:

>Translated_345_residues
MADKQKHHTLESIAKLAGVSRSTVSRVINNKGNVSEKARKKVLSVIKKEKYYPNASARSLASRKTNNIGIIFWGHDPEFL
SNPIYYEILQGIQRESLKHDLNLVLYTTHKEEEKRQELCYKIIGQKNVDGLIVIGSLMYFDYLKLFANNDLPVVIIGKRD
TKNLDIPYVCTDYEDGIYKATRHLLSLGRKEIVLLQSTVDYYFLEDKLRGYKRALEEKNIKFNSDYIIEVTSNNEDYVEK
IVRNHILTIKNINAIICANDILAQNLIKVITSQGIAVPDNISVIGFNDINTARYFNPPLTTVKQNKIKLGINAVRTLLRK
INGEETGNVVLDAELVVRGSTAPLK
>Mature_344_residues
ADKQKHHTLESIAKLAGVSRSTVSRVINNKGNVSEKARKKVLSVIKKEKYYPNASARSLASRKTNNIGIIFWGHDPEFLS
NPIYYEILQGIQRESLKHDLNLVLYTTHKEEEKRQELCYKIIGQKNVDGLIVIGSLMYFDYLKLFANNDLPVVIIGKRDT
KNLDIPYVCTDYEDGIYKATRHLLSLGRKEIVLLQSTVDYYFLEDKLRGYKRALEEKNIKFNSDYIIEVTSNNEDYVEKI
VRNHILTIKNINAIICANDILAQNLIKVITSQGIAVPDNISVIGFNDINTARYFNPPLTTVKQNKIKLGINAVRTLLRKI
NGEETGNVVLDAELVVRGSTAPLK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=26.9230769230769, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=29.585798816568, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789202, Length=297, Percent_Identity=31.3131313131313, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=28.2131661442006, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=29.4670846394984, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789068, Length=301, Percent_Identity=28.5714285714286, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=30.2325581395349, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786540, Length=346, Percent_Identity=27.4566473988439, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI48994940, Length=329, Percent_Identity=24.9240121580547, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787906, Length=343, Percent_Identity=25.3644314868805, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790715, Length=338, Percent_Identity=24.8520710059172, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786268, Length=304, Percent_Identity=25, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39198; Mature: 39067

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKQKHHTLESIAKLAGVSRSTVSRVINNKGNVSEKARKKVLSVIKKEKYYPNASARSL
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
ASRKTNNIGIIFWGHDPEFLSNPIYYEILQGIQRESLKHDLNLVLYTTHKEEEKRQELCY
HHCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
KIIGQKNVDGLIVIGSLMYFDYLKLFANNDLPVVIIGKRDTKNLDIPYVCTDYEDGIYKA
HHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEECCCCCHHHHH
TRHLLSLGRKEIVLLQSTVDYYFLEDKLRGYKRALEEKNIKFNSDYIIEVTSNNEDYVEK
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCHHHHHH
IVRNHILTIKNINAIICANDILAQNLIKVITSQGIAVPDNISVIGFNDINTARYFNPPLT
HHHHCEEEEECCCEEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCC
TVKQNKIKLGINAVRTLLRKINGEETGNVVLDAELVVRGSTAPLK
CCCCCCEEECHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
ADKQKHHTLESIAKLAGVSRSTVSRVINNKGNVSEKARKKVLSVIKKEKYYPNASARSL
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
ASRKTNNIGIIFWGHDPEFLSNPIYYEILQGIQRESLKHDLNLVLYTTHKEEEKRQELCY
HHCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
KIIGQKNVDGLIVIGSLMYFDYLKLFANNDLPVVIIGKRDTKNLDIPYVCTDYEDGIYKA
HHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEECCCCCHHHHH
TRHLLSLGRKEIVLLQSTVDYYFLEDKLRGYKRALEEKNIKFNSDYIIEVTSNNEDYVEK
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCHHHHHH
IVRNHILTIKNINAIICANDILAQNLIKVITSQGIAVPDNISVIGFNDINTARYFNPPLT
HHHHCEEEEECCCEEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCC
TVKQNKIKLGINAVRTLLRKINGEETGNVVLDAELVVRGSTAPLK
CCCCCCEEECHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA