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The map label for this gene is ppsA [H]

Identifier: 220932109

GI number: 220932109

Start: 1363497

End: 1365791

Strand: Reverse

Name: ppsA [H]

Synonym: Hore_12720

Alternate gene names: 220932109

Gene position: 1365791-1363497 (Counterclockwise)

Preceding gene: 220932110

Following gene: 220932108

Centisome position: 52.98

GC content: 38.56

Gene sequence:

>2295_bases
ATGAAGTACATTAAATGGTTTGAAGAGCTAACAGGAAAGGATGTAAACAGGGTGGGAGGAAAGGGGGCCAATCTCGGGGA
ATTATCACAGCTAGGGGTGTCAGTGCCTCCGGGTTTTTGTGTAACAGTAGACGCCTATCACCGTTTTATAAAAGAACAGG
GCCTCGATAAAGTAATTGCCACTGAACTAAGAAGAGTTAATAGAGACGATATGGAATCATTAAAAAAAATATCAACAAAA
ATTAGAAATATTATTATTTCTTCTAAATTAAGCACACAGTTTGAACGGAAGTTAAAAGAAAACTATAATAAGTTGATTGA
TGTATCTGGTGGTAATTCGTTTGTGGCTGTTAGAAGTTCGGCTACTGCAGAAGATTTACCAGAAGCATCTTTTGCCGGGC
AGCAGGATACCTACCTTTATGTAAATGGTATGGCAGAGTTAAAAGAGAAAATTAAGATGTGCTGGGCTTCCCTGTGGACA
GTCAGGGCCATATTTTATCGTGAAGAAAATGGTTTTGAGCATCCTGGGGTAGGCCTCAGTGCTGTAGTTCAATTAATGGT
AAATAGTAAAAAATCAGGTGTTCTTTTTACAGTTAATCCGGTAACGAATAATGATACTGAAATAATGATTAATTCGTCCT
GGGGTCTGGGAGAGGCTATTGTATCTGGCATGGTAACCCCTGATGAATTTATAGTTGATAAAAGGAACATGAAGGTAATT
GAACAAAATCCGGGAACTAAAAACAATATGGTTGTCCAGAATACAAAGGCAATGTCAGGAACTGAAGTTGTAGATATTTC
TACCTATCTGGGGATAGATAAGGTTAAAAAACTTTCTTTAAAACCGGAGGAAATAAGAGAGTTGGTGGAGGCCGCCCTCA
AAATCGAAAATCATTATGGTAGACCCCAGGATATAGAATGGGCATATACTGATAGACTATATATCTTACAGGCAAGACCA
ATAACTACTTTAAAACCCAGAAAAAAGTCACTAAAACCATTGGTTAAAGGGTTAAGTGCCTCTCCAGGCATAGGGAAGGG
AAAAGTTAAGATAATTAATGATATGGAAAAATTAAACGAGGTAAAAGAAGGCGATATATTAGTTACAGTAATGACAAACC
CGGATATGGTACCGGTTATGAGGAAGGTGGCAGCAGTAGTTACTGATGAGGGAGGGCGGACCTGTCATGCAGCAATAGTA
TCCCGGGAACTGGGAATTCCCTGTATTGTTGGGGCAGGAAAAGCGACCGATATTTTGAAGGATGGAATGAAGGTAACAGT
TGATGCTACCAGAGGAACAGTATTTTCCGGGTTTAATTTTGAGACCAGTAAGGAAAGATTAAATAATAATAAATCACATA
ACTATGCTGGTTCCGGGTATGTTCCTGTAACAGCTACAAAGGTATATATGAACCTGGGGGAACCAAATCTTATTGATAGA
TACAAAGATATTCCCTTTGATGGTATTGGCCTTATGAGAACTGAGTTTATATTCTCAAATTATGTCGGGGTACACCCCCT
TTATTTACTTAAAAAAGGACAGGGGAATATCCTGATCCGTAAAATGGCAGAAGCTGTTTCTAAAGTTGCCAGAAAAATAT
ATCCACGGAAACTTGTAGTCAGGCTCAGTGATTTCAGGACAAATGAATTTCGGGGACTGGAAGGAGGAGAAGAGGTAGAA
CCGGTTGAAAATAACCCCATGATCGGGTGGCGTGGTGTTGCCCGTTACATTTCCTCCAGATATGAAAAGGGTTTCAGGCT
GGAATGTAAAGCCATAAAAAAGGTCAGAGATGACTATGGCTTGAATAATGTATATGTAATGCTTCCCTTTGTCAGAACAA
CATGGGAAGTTAAAAAGGTTCTTAAGATACTGGCAGAGGAAGGTTTAGAGAGGGGTCCAGATTTTAAAGTCTGGATAATG
GCTGAAGTACCGTCTGTTATTTTTCTTGCTGAGGAATTTTCCAGGCTAATTGATGGATTCAGTATAGGAAGTAACGATTT
GACCCAACTTATTATTGGAGCTGACAGGGATTCAGGAATATTAAATCAGATGGGGTATTTTGATGAAAGGGATCCTGCAG
TTAAAATGGCAATAAAACGACTTATAACAATAGCGCACAGATATGATAAAACGGTTTCGATATGTGGTCAGGGTCCTTCA
ACCTATCCTGAATTAACCGAGTTCTTAGTTCAAGAGGGTATTGACAGTATAAGTGTTAATCCGGATACAGTTTTTGAGAC
AAAAAAGTTAGTTGCATCTATTGAGAAGAAAATAATTTTAAATAAGTTAAGATAA

Upstream 100 bases:

>100_bases
GTACAATATAAAGAGGGAAACTCAAAAAAGTGTAGAATATAAAAAATAAAGTATACGTTATAAAATAAAAATATTTATGA
AAGAGGTGTAATACTATTAA

Downstream 100 bases:

>100_bases
TTTTAAATAAGTTAAGAGGGTGAGTAATATAATGAATCTATCTGAAAGACAGAAGGAGATAATTCGTATCGTAAAACAGA
ATGAACCTATTACCAGTCAG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKYIKWFEELTGKDVNRVGGKGANLGELSQLGVSVPPGFCVTVDAYHRFIKEQGLDKVIATELRRVNRDDMESLKKISTK
IRNIIISSKLSTQFERKLKENYNKLIDVSGGNSFVAVRSSATAEDLPEASFAGQQDTYLYVNGMAELKEKIKMCWASLWT
VRAIFYREENGFEHPGVGLSAVVQLMVNSKKSGVLFTVNPVTNNDTEIMINSSWGLGEAIVSGMVTPDEFIVDKRNMKVI
EQNPGTKNNMVVQNTKAMSGTEVVDISTYLGIDKVKKLSLKPEEIRELVEAALKIENHYGRPQDIEWAYTDRLYILQARP
ITTLKPRKKSLKPLVKGLSASPGIGKGKVKIINDMEKLNEVKEGDILVTVMTNPDMVPVMRKVAAVVTDEGGRTCHAAIV
SRELGIPCIVGAGKATDILKDGMKVTVDATRGTVFSGFNFETSKERLNNNKSHNYAGSGYVPVTATKVYMNLGEPNLIDR
YKDIPFDGIGLMRTEFIFSNYVGVHPLYLLKKGQGNILIRKMAEAVSKVARKIYPRKLVVRLSDFRTNEFRGLEGGEEVE
PVENNPMIGWRGVARYISSRYEKGFRLECKAIKKVRDDYGLNNVYVMLPFVRTTWEVKKVLKILAEEGLERGPDFKVWIM
AEVPSVIFLAEEFSRLIDGFSIGSNDLTQLIIGADRDSGILNQMGYFDERDPAVKMAIKRLITIAHRYDKTVSICGQGPS
TYPELTEFLVQEGIDSISVNPDTVFETKKLVASIEKKIILNKLR

Sequences:

>Translated_764_residues
MKYIKWFEELTGKDVNRVGGKGANLGELSQLGVSVPPGFCVTVDAYHRFIKEQGLDKVIATELRRVNRDDMESLKKISTK
IRNIIISSKLSTQFERKLKENYNKLIDVSGGNSFVAVRSSATAEDLPEASFAGQQDTYLYVNGMAELKEKIKMCWASLWT
VRAIFYREENGFEHPGVGLSAVVQLMVNSKKSGVLFTVNPVTNNDTEIMINSSWGLGEAIVSGMVTPDEFIVDKRNMKVI
EQNPGTKNNMVVQNTKAMSGTEVVDISTYLGIDKVKKLSLKPEEIRELVEAALKIENHYGRPQDIEWAYTDRLYILQARP
ITTLKPRKKSLKPLVKGLSASPGIGKGKVKIINDMEKLNEVKEGDILVTVMTNPDMVPVMRKVAAVVTDEGGRTCHAAIV
SRELGIPCIVGAGKATDILKDGMKVTVDATRGTVFSGFNFETSKERLNNNKSHNYAGSGYVPVTATKVYMNLGEPNLIDR
YKDIPFDGIGLMRTEFIFSNYVGVHPLYLLKKGQGNILIRKMAEAVSKVARKIYPRKLVVRLSDFRTNEFRGLEGGEEVE
PVENNPMIGWRGVARYISSRYEKGFRLECKAIKKVRDDYGLNNVYVMLPFVRTTWEVKKVLKILAEEGLERGPDFKVWIM
AEVPSVIFLAEEFSRLIDGFSIGSNDLTQLIIGADRDSGILNQMGYFDERDPAVKMAIKRLITIAHRYDKTVSICGQGPS
TYPELTEFLVQEGIDSISVNPDTVFETKKLVASIEKKIILNKLR
>Mature_764_residues
MKYIKWFEELTGKDVNRVGGKGANLGELSQLGVSVPPGFCVTVDAYHRFIKEQGLDKVIATELRRVNRDDMESLKKISTK
IRNIIISSKLSTQFERKLKENYNKLIDVSGGNSFVAVRSSATAEDLPEASFAGQQDTYLYVNGMAELKEKIKMCWASLWT
VRAIFYREENGFEHPGVGLSAVVQLMVNSKKSGVLFTVNPVTNNDTEIMINSSWGLGEAIVSGMVTPDEFIVDKRNMKVI
EQNPGTKNNMVVQNTKAMSGTEVVDISTYLGIDKVKKLSLKPEEIRELVEAALKIENHYGRPQDIEWAYTDRLYILQARP
ITTLKPRKKSLKPLVKGLSASPGIGKGKVKIINDMEKLNEVKEGDILVTVMTNPDMVPVMRKVAAVVTDEGGRTCHAAIV
SRELGIPCIVGAGKATDILKDGMKVTVDATRGTVFSGFNFETSKERLNNNKSHNYAGSGYVPVTATKVYMNLGEPNLIDR
YKDIPFDGIGLMRTEFIFSNYVGVHPLYLLKKGQGNILIRKMAEAVSKVARKIYPRKLVVRLSDFRTNEFRGLEGGEEVE
PVENNPMIGWRGVARYISSRYEKGFRLECKAIKKVRDDYGLNNVYVMLPFVRTTWEVKKVLKILAEEGLERGPDFKVWIM
AEVPSVIFLAEEFSRLIDGFSIGSNDLTQLIIGADRDSGILNQMGYFDERDPAVKMAIKRLITIAHRYDKTVSICGQGPS
TYPELTEFLVQEGIDSISVNPDTVFETKKLVASIEKKIILNKLR

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=801, Percent_Identity=41.8227215980025, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1788756, Length=426, Percent_Identity=25.3521126760563, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788726, Length=398, Percent_Identity=23.6180904522613, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1789193, Length=405, Percent_Identity=25.1851851851852, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI48994992, Length=245, Percent_Identity=28.1632653061224, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=396, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 85451; Mature: 85451

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIKWFEELTGKDVNRVGGKGANLGELSQLGVSVPPGFCVTVDAYHRFIKEQGLDKVIA
CCHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCHHHHH
TELRRVNRDDMESLKKISTKIRNIIISSKLSTQFERKLKENYNKLIDVSGGNSFVAVRSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCEEEEEECC
ATAEDLPEASFAGQQDTYLYVNGMAELKEKIKMCWASLWTVRAIFYREENGFEHPGVGLS
CCHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHH
AVVQLMVNSKKSGVLFTVNPVTNNDTEIMINSSWGLGEAIVSGMVTPDEFIVDKRNMKVI
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHEECCCCCEEE
EQNPGTKNNMVVQNTKAMSGTEVVDISTYLGIDKVKKLSLKPEEIRELVEAALKIENHYG
ECCCCCCCCEEEEECCCCCCCEEEEEHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
RPQDIEWAYTDRLYILQARPITTLKPRKKSLKPLVKGLSASPGIGKGKVKIINDMEKLNE
CCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHH
VKEGDILVTVMTNPDMVPVMRKVAAVVTDEGGRTCHAAIVSRELGIPCIVGAGKATDILK
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCEEEECCCHHHHHH
DGMKVTVDATRGTVFSGFNFETSKERLNNNKSHNYAGSGYVPVTATKVYMNLGEPNLIDR
CCCEEEEEECCCEEEECCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHH
YKDIPFDGIGLMRTEFIFSNYVGVHPLYLLKKGQGNILIRKMAEAVSKVARKIYPRKLVV
HHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHEEE
RLSDFRTNEFRGLEGGEEVEPVENNPMIGWRGVARYISSRYEKGFRLECKAIKKVRDDYG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCC
LNNVYVMLPFVRTTWEVKKVLKILAEEGLERGPDFKVWIMAEVPSVIFLAEEFSRLIDGF
CCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCC
SIGSNDLTQLIIGADRDSGILNQMGYFDERDPAVKMAIKRLITIAHRYDKTVSICGQGPS
CCCCCCCEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
TYPELTEFLVQEGIDSISVNPDTVFETKKLVASIEKKIILNKLR
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYIKWFEELTGKDVNRVGGKGANLGELSQLGVSVPPGFCVTVDAYHRFIKEQGLDKVIA
CCHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCHHHHH
TELRRVNRDDMESLKKISTKIRNIIISSKLSTQFERKLKENYNKLIDVSGGNSFVAVRSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCEEEEEECC
ATAEDLPEASFAGQQDTYLYVNGMAELKEKIKMCWASLWTVRAIFYREENGFEHPGVGLS
CCHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHH
AVVQLMVNSKKSGVLFTVNPVTNNDTEIMINSSWGLGEAIVSGMVTPDEFIVDKRNMKVI
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHEECCCCCEEE
EQNPGTKNNMVVQNTKAMSGTEVVDISTYLGIDKVKKLSLKPEEIRELVEAALKIENHYG
ECCCCCCCCEEEEECCCCCCCEEEEEHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
RPQDIEWAYTDRLYILQARPITTLKPRKKSLKPLVKGLSASPGIGKGKVKIINDMEKLNE
CCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHH
VKEGDILVTVMTNPDMVPVMRKVAAVVTDEGGRTCHAAIVSRELGIPCIVGAGKATDILK
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCEEEECCCHHHHHH
DGMKVTVDATRGTVFSGFNFETSKERLNNNKSHNYAGSGYVPVTATKVYMNLGEPNLIDR
CCCEEEEEECCCEEEECCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHH
YKDIPFDGIGLMRTEFIFSNYVGVHPLYLLKKGQGNILIRKMAEAVSKVARKIYPRKLVV
HHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHEEE
RLSDFRTNEFRGLEGGEEVEPVENNPMIGWRGVARYISSRYEKGFRLECKAIKKVRDDYG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCC
LNNVYVMLPFVRTTWEVKKVLKILAEEGLERGPDFKVWIMAEVPSVIFLAEEFSRLIDGF
CCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCC
SIGSNDLTQLIIGADRDSGILNQMGYFDERDPAVKMAIKRLITIAHRYDKTVSICGQGPS
CCCCCCCEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
TYPELTEFLVQEGIDSISVNPDTVFETKKLVASIEKKIILNKLR
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]