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The map label for this gene is dnaG [H]

Identifier: 220932078

GI number: 220932078

Start: 1334599

End: 1336398

Strand: Reverse

Name: dnaG [H]

Synonym: Hore_12410

Alternate gene names: 220932078

Gene position: 1336398-1334599 (Counterclockwise)

Preceding gene: 220932079

Following gene: 220932077

Centisome position: 51.84

GC content: 33.78

Gene sequence:

>1800_bases
GTGGGGAGTTCTTTTGAAAAATTTTTAGATGAACTAAAAGGCTCACTTGATATTGTAGATCTAGTTTCAGACTATGTGGA
TTTAAAGAAGGTTGGTAAAAATTATGTTGGCCTGTGTCCATTTCACCAGGAAAAAACACCTTCCTTTACTGTAAACCCCG
AGAGGCAGTTTTTTTACTGTTTTGGTTGTGGTGTCGGTGGGGATGTAATTAGTTTCTTGATGGAGATAGAAAATATAACT
TTTAAAGAAAGTGTTAAAATGCTGGCCAGGAGGTCCGGGATGGAGGTCCCTGGAGATGATCCCCGCCACAGACAACAATT
AAGAGAGAGAGAAAAGCTTTTTAAAATTAACCATCTTACTGCAAAATTTTACCATTACCTGTTGTTAGAAACTGACCAGG
GCTTGAAAGCAATAAATTATCTCAAAAAAAGGGGTTTTACTAAAAAAGATATTAAGAAATTTGGACTCGGTTATGCCCCG
GACCGGTGGGATGGGCTTTTAAAGTTTTTAACCGGTAAAGGATTTGAGGAGGGAGAATTGGTGAAGGCTGGCCTTGTCCT
GTTAAGTAATAACAATAATTATTATGATAGGTTCAGGGACAGGCTTATTTTTCCCATATTTAATAGCCGGGGAGAAGTTC
TGGCTTTTGGGGGTAGATTAATTGATAGTGATGAATCTCAACCCAAGTATTTAAATTCTCCTGAAACACCTGTTTTCAAT
AAAGGTAAAAACCTTTATGGTTTAAACTGGGCCAAACATGAAATGAGAAAAAGTAGCGAAGTAATAATTATGGAAGGTTA
TACTGATGTTTTGCGTGCCCATCAGATGGGTATCAATAATGTGGTTGCTTCTCTGGGGACAGCCCTTACTTCTGAACAGG
CCCGGTTGATAGCAAGGTATTCCAATCTTGCTTATATTTCATATGATGCTGATACTGCAGGCACTAAAGCAACTTTGAGA
GGGCTTGATATTCTTAAAAATGCTGGTCTTAACGTAAAAGTGGTTGATTTGCCACAGGATAGTGACCCTGATGAATATTT
AAGAAAAAAGGGTGTAGATGAATTTTATACCCTGTTAGAAGAAGCGGTTGATTTAGTAGAGTTTAAAATCAAGCAGGGCC
TTGAAAATATTGATCTTAACAATGTTCAGGGTAAAATAGAAGGTACCAGAAAGCTGGTAAAAATTCTTGCTACATTAGAG
GACCGGATTGAAAGAGAGATATATATACAAAATGTTTCCGATAAATTCAATCTGGATCCAGAAATATTAAAGAGTGAAGT
CGAGAAATTGATTAAAATTAATAAAAATAAGGATAAAAATTCATCAAGAAGATATACTAAAAAAAGTAATAGAAGATATT
TCACTAACAAGAATAATAAGACAAATTCCTTTAGGAGTATACATTTTATAGAAGAGAAACTTTTACACGCTTTTATAGAT
TTCCCTGAATTAAGGAATACTATTGTTAATGAAATTAATACTGATATGTTTTCTAAAGAGTATCAAGAGGTGGCAGAGTT
TTTATGGGAAAATAAGGATTCATTAAAAGGAGATATTATTAATAATATTGAAAGTAAAGTTTTAAGAAATAAAGTTATGG
AACTTATGATTAATAATAAAACTGTCCAACCTGAAGGGGTTGAGAGGTTAATAGTTAACTTCAAAGAGGAAAATGTATAT
AATATAAAGGTTGGTTTATATAAAAAACTTCAGGAAAATAACCTTAATGTCAATAGATTAAATAAATTACTGATTAATTT
TCAGAAGTTGTCAGGGAATCACAGAAAGGAGGGATTGTAA

Upstream 100 bases:

>100_bases
ATGAATAATTGATAAGGGTGTCTAGTGGGACACAGGGAAGGAATTAGGATAGTTTTAACGAAGTATTAAAAATAGGGTAG
AATTGTTAGGGTGAAGACAG

Downstream 100 bases:

>100_bases
TGGGTAAAAATTTCAATCCAGACAAAGTTAAAGAAGTTAAGGAATTAATTAAAAAAGGTAAAGAAGAAGGCTATTTGACC
TATGAAGAAATTATGGATTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MGSSFEKFLDELKGSLDIVDLVSDYVDLKKVGKNYVGLCPFHQEKTPSFTVNPERQFFYCFGCGVGGDVISFLMEIENIT
FKESVKMLARRSGMEVPGDDPRHRQQLREREKLFKINHLTAKFYHYLLLETDQGLKAINYLKKRGFTKKDIKKFGLGYAP
DRWDGLLKFLTGKGFEEGELVKAGLVLLSNNNNYYDRFRDRLIFPIFNSRGEVLAFGGRLIDSDESQPKYLNSPETPVFN
KGKNLYGLNWAKHEMRKSSEVIIMEGYTDVLRAHQMGINNVVASLGTALTSEQARLIARYSNLAYISYDADTAGTKATLR
GLDILKNAGLNVKVVDLPQDSDPDEYLRKKGVDEFYTLLEEAVDLVEFKIKQGLENIDLNNVQGKIEGTRKLVKILATLE
DRIEREIYIQNVSDKFNLDPEILKSEVEKLIKINKNKDKNSSRRYTKKSNRRYFTNKNNKTNSFRSIHFIEEKLLHAFID
FPELRNTIVNEINTDMFSKEYQEVAEFLWENKDSLKGDIINNIESKVLRNKVMELMINNKTVQPEGVERLIVNFKEENVY
NIKVGLYKKLQENNLNVNRLNKLLINFQKLSGNHRKEGL

Sequences:

>Translated_599_residues
MGSSFEKFLDELKGSLDIVDLVSDYVDLKKVGKNYVGLCPFHQEKTPSFTVNPERQFFYCFGCGVGGDVISFLMEIENIT
FKESVKMLARRSGMEVPGDDPRHRQQLREREKLFKINHLTAKFYHYLLLETDQGLKAINYLKKRGFTKKDIKKFGLGYAP
DRWDGLLKFLTGKGFEEGELVKAGLVLLSNNNNYYDRFRDRLIFPIFNSRGEVLAFGGRLIDSDESQPKYLNSPETPVFN
KGKNLYGLNWAKHEMRKSSEVIIMEGYTDVLRAHQMGINNVVASLGTALTSEQARLIARYSNLAYISYDADTAGTKATLR
GLDILKNAGLNVKVVDLPQDSDPDEYLRKKGVDEFYTLLEEAVDLVEFKIKQGLENIDLNNVQGKIEGTRKLVKILATLE
DRIEREIYIQNVSDKFNLDPEILKSEVEKLIKINKNKDKNSSRRYTKKSNRRYFTNKNNKTNSFRSIHFIEEKLLHAFID
FPELRNTIVNEINTDMFSKEYQEVAEFLWENKDSLKGDIINNIESKVLRNKVMELMINNKTVQPEGVERLIVNFKEENVY
NIKVGLYKKLQENNLNVNRLNKLLINFQKLSGNHRKEGL
>Mature_598_residues
GSSFEKFLDELKGSLDIVDLVSDYVDLKKVGKNYVGLCPFHQEKTPSFTVNPERQFFYCFGCGVGGDVISFLMEIENITF
KESVKMLARRSGMEVPGDDPRHRQQLREREKLFKINHLTAKFYHYLLLETDQGLKAINYLKKRGFTKKDIKKFGLGYAPD
RWDGLLKFLTGKGFEEGELVKAGLVLLSNNNNYYDRFRDRLIFPIFNSRGEVLAFGGRLIDSDESQPKYLNSPETPVFNK
GKNLYGLNWAKHEMRKSSEVIIMEGYTDVLRAHQMGINNVVASLGTALTSEQARLIARYSNLAYISYDADTAGTKATLRG
LDILKNAGLNVKVVDLPQDSDPDEYLRKKGVDEFYTLLEEAVDLVEFKIKQGLENIDLNNVQGKIEGTRKLVKILATLED
RIEREIYIQNVSDKFNLDPEILKSEVEKLIKINKNKDKNSSRRYTKKSNRRYFTNKNNKTNSFRSIHFIEEKLLHAFIDF
PELRNTIVNEINTDMFSKEYQEVAEFLWENKDSLKGDIINNIESKVLRNKVMELMINNKTVQPEGVERLIVNFKEENVYN
IKVGLYKKLQENNLNVNRLNKLLINFQKLSGNHRKEGL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=430, Percent_Identity=36.7441860465116, Blast_Score=292, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69183; Mature: 69052

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSFEKFLDELKGSLDIVDLVSDYVDLKKVGKNYVGLCPFHQEKTPSFTVNPERQFFYC
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECCCCCEEEE
FGCGVGGDVISFLMEIENITFKESVKMLARRSGMEVPGDDPRHRQQLREREKLFKINHLT
EECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AKFYHYLLLETDQGLKAINYLKKRGFTKKDIKKFGLGYAPDRWDGLLKFLTGKGFEEGEL
HHHHHHHEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCE
VKAGLVLLSNNNNYYDRFRDRLIFPIFNSRGEVLAFGGRLIDSDESQPKYLNSPETPVFN
EEEEEEEEECCCCHHHHHHHCEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCCCCCC
KGKNLYGLNWAKHEMRKSSEVIIMEGYTDVLRAHQMGINNVVASLGTALTSEQARLIARY
CCCCEECCCHHHHHHCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SNLAYISYDADTAGTKATLRGLDILKNAGLNVKVVDLPQDSDPDEYLRKKGVDEFYTLLE
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHH
EAVDLVEFKIKQGLENIDLNNVQGKIEGTRKLVKILATLEDRIEREIYIQNVSDKFNLDP
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCH
EILKSEVEKLIKINKNKDKNSSRRYTKKSNRRYFTNKNNKTNSFRSIHFIEEKLLHAFID
HHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHC
FPELRNTIVNEINTDMFSKEYQEVAEFLWENKDSLKGDIINNIESKVLRNKVMELMINNK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TVQPEGVERLIVNFKEENVYNIKVGLYKKLQENNLNVNRLNKLLINFQKLSGNHRKEGL
CCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GSSFEKFLDELKGSLDIVDLVSDYVDLKKVGKNYVGLCPFHQEKTPSFTVNPERQFFYC
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECCCCCEEEE
FGCGVGGDVISFLMEIENITFKESVKMLARRSGMEVPGDDPRHRQQLREREKLFKINHLT
EECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AKFYHYLLLETDQGLKAINYLKKRGFTKKDIKKFGLGYAPDRWDGLLKFLTGKGFEEGEL
HHHHHHHEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCE
VKAGLVLLSNNNNYYDRFRDRLIFPIFNSRGEVLAFGGRLIDSDESQPKYLNSPETPVFN
EEEEEEEEECCCCHHHHHHHCEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCCCCCC
KGKNLYGLNWAKHEMRKSSEVIIMEGYTDVLRAHQMGINNVVASLGTALTSEQARLIARY
CCCCEECCCHHHHHHCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SNLAYISYDADTAGTKATLRGLDILKNAGLNVKVVDLPQDSDPDEYLRKKGVDEFYTLLE
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHH
EAVDLVEFKIKQGLENIDLNNVQGKIEGTRKLVKILATLEDRIEREIYIQNVSDKFNLDP
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCH
EILKSEVEKLIKINKNKDKNSSRRYTKKSNRRYFTNKNNKTNSFRSIHFIEEKLLHAFID
HHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHC
FPELRNTIVNEINTDMFSKEYQEVAEFLWENKDSLKGDIINNIESKVLRNKVMELMINNK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TVQPEGVERLIVNFKEENVYNIKVGLYKKLQENNLNVNRLNKLLINFQKLSGNHRKEGL
CCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]