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The map label for this gene is ruvC

Identifier: 220932070

GI number: 220932070

Start: 1326554

End: 1327051

Strand: Reverse

Name: ruvC

Synonym: Hore_12330

Alternate gene names: 220932070

Gene position: 1327051-1326554 (Counterclockwise)

Preceding gene: 220932071

Following gene: 220932069

Centisome position: 51.47

GC content: 40.36

Gene sequence:

>498_bases
TTGGTTATTTTAGGAGTTGACCCGGGCCTGGCAATAGTCGGGTATGCTCTGATCGAAAAGCAGGGCAATAAATATAGAGT
AATAGATTATGGTTCTATTAATACTCCATCAAAGCTAGAAAGTGTTGACAGGCTCAAGATAATACATACAGATATGGTTA
ATATAATAAATAAATATAATCCTGATCAGATGGCTGTAGAGAAACTTTTTTTTAATAAAAACGTGAAAACAGCCATTGAA
GTAGGTCAGGCACGGGGAGTTATTTTGCTGGCAGGATCCCAGGCCCGTCTTAAAATTTATGAATATACCCCGCTTCAGGT
AAAGCAGGCTGTTGCCGGTTATGGTCGGGCCCATAAAAGCCAGGTTCAGCGAATGGTAAAAGCCCTGTTAAATTTAAACG
AAATACCAAAACCTGATGATGTAGCTGATGCCCTGGCTATTTCTATTTGCCATGGAAACAGTTACCGCTTAAATCGGAAA
TGGGGGACAAAAGGGTGA

Upstream 100 bases:

>100_bases
AGGTAATACCAGTAAATCTGTTTATAAATCCAGAAGGAATTAATTAACACTTTACAGAAATATATAAACAAACATATATT
AGTATTAATGGGGTGGTACG

Downstream 100 bases:

>100_bases
TAGGTTATCTAAAAGGGAATGTTATCTGGAAGGCTGAAAATAAAGTAATTCTGGAGACAGGAGGGGTAGGTTATCGGGTT
TTAGTACCATCTACTGTCAG

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MVILGVDPGLAIVGYALIEKQGNKYRVIDYGSINTPSKLESVDRLKIIHTDMVNIINKYNPDQMAVEKLFFNKNVKTAIE
VGQARGVILLAGSQARLKIYEYTPLQVKQAVAGYGRAHKSQVQRMVKALLNLNEIPKPDDVADALAISICHGNSYRLNRK
WGTKG

Sequences:

>Translated_165_residues
MVILGVDPGLAIVGYALIEKQGNKYRVIDYGSINTPSKLESVDRLKIIHTDMVNIINKYNPDQMAVEKLFFNKNVKTAIE
VGQARGVILLAGSQARLKIYEYTPLQVKQAVAGYGRAHKSQVQRMVKALLNLNEIPKPDDVADALAISICHGNSYRLNRK
WGTKG
>Mature_165_residues
MVILGVDPGLAIVGYALIEKQGNKYRVIDYGSINTPSKLESVDRLKIIHTDMVNIINKYNPDQMAVEKLFFNKNVKTAIE
VGQARGVILLAGSQARLKIYEYTPLQVKQAVAGYGRAHKSQVQRMVKALLNLNEIPKPDDVADALAISICHGNSYRLNRK
WGTKG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=137, Percent_Identity=41.6058394160584, Blast_Score=111, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_HALOH (B8CXG4)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508978.1
- ProteinModelPortal:   B8CXG4
- SMR:   B8CXG4
- GeneID:   7313934
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_12330
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18308; Mature: 18308

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVILGVDPGLAIVGYALIEKQGNKYRVIDYGSINTPSKLESVDRLKIIHTDMVNIINKYN
CEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PDQMAVEKLFFNKNVKTAIEVGQARGVILLAGSQARLKIYEYTPLQVKQAVAGYGRAHKS
CHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHCCCHHHH
QVQRMVKALLNLNEIPKPDDVADALAISICHGNSYRLNRKWGTKG
HHHHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCEEEECCCCCCC
>Mature Secondary Structure
MVILGVDPGLAIVGYALIEKQGNKYRVIDYGSINTPSKLESVDRLKIIHTDMVNIINKYN
CEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PDQMAVEKLFFNKNVKTAIEVGQARGVILLAGSQARLKIYEYTPLQVKQAVAGYGRAHKS
CHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHCCCHHHH
QVQRMVKALLNLNEIPKPDDVADALAISICHGNSYRLNRKWGTKG
HHHHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA