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The map label for this gene is miaB [H]

Identifier: 220932005

GI number: 220932005

Start: 1261147

End: 1262499

Strand: Reverse

Name: miaB [H]

Synonym: Hore_11680

Alternate gene names: 220932005

Gene position: 1262499-1261147 (Counterclockwise)

Preceding gene: 220932006

Following gene: 220932004

Centisome position: 48.97

GC content: 38.21

Gene sequence:

>1353_bases
ATGAATAACAGAAAATACTTTATTTTGACATATGGATGTCAGATGAATGTTCATGATTCTGAAAAACTGGCCGGAATGCT
TGAAGAAATGGGTTATAAGTCTACAAATAATTTAGAAGAAGCCGATATTATTATGGTAAATACATGTGCTGTAAGAGAAA
ATGCAGAATTGAGGGTTTTTGGCAGGGTCGGAGATTTTAAGAGATTAAAAGAGAAAAACCCTGATTTAATAATTGGAGTC
GGCGGCTGTATGATGCAGATAGATGAAAATGCCAGAAAGCTTTATGAAAAATACCCCCATGTCGATCTCATATTTGGTAC
ACATAATATTCACCATATCCCTGAACTAATAAAACGAATTAAAGAAGAAAGGGGACGTATCATTGAGGTCTGGAATCAGG
AAGAGGGGTTAATTCCTGATATTCCCTATAAAAGGGAGGATGACTTTAAGGCCTGGATCTCCATTATACAGGGGTGTAAC
AATTTCTGCACCTATTGTATTGTCCCTTATGTCCGGGGCCGGGAACGGAGCAGGCCTGCCGCTGATATAATTTCTGAAGC
CCGTAAATTAGCCAGTGAAGGGGTTAAAGAGATAACCCTTCTGGGGCAGAATGTTAATTCCTATGGAAAGGATTTAAAGG
AAGATATAGATTTTGCTGATCTGCTTAAAAGACTTAACAGGGTTGAAGGGATTAAGAGAATAAGATATATGACCTCTCAT
CCCCGGGATTTCAGTGATAAAATGATTAAAATTATAAAAGAGTGTGACAAAGTATGTGAACACTTTCATCTCCCTGTCCA
GTCGGGTAGCACCAGAATCCTGAAAAAAATGAACCGTGGGTATACCCAGGCAGAATATTTAAATCTGATTAAAAAAATAA
AGAGTCAAATCCCGGATTACAGTATTACTACTGATATTATTGTAGGATTCCCCGGTGAAACAGAAGAGGATTTCCAGGAA
ACCTTAAAAGTCATCAGGGAAGTGAGATTTGATATGGCTTATACATTCAAATATTCACCCCGGAAGGGAACTCCGGCTGC
CAGACATAAAGACCAGGTTAGTGAAAAGATTAAGCAGGATAGATTAACCCGCTTAATTGAGGTCCAGAATAGTATCAGTT
TAGAAAACAACAGGAAACTCAAGGGTAAAACAGTAGAGGTTTTAATAGAGGGTGAAAGTAGAAATAACCCCGATACTTTT
GAAGGCAGGACCAGAACCAATAAACTGGTTATAGTCCCCAGAAATGAAAACCTGAAGGGTCAAATAGCAAATGTTAAAAT
AAACAGGGTTGGAAGCTGGACATTATATGGTGAGGTTATAGATAATACCCGGGAAACTGTCTTAAATACCTGA

Upstream 100 bases:

>100_bases
GATTTAAAGACTTATTTTTTTTATTATGTAATGATTTTACTTTAATTTGATAAAATAATGTAATATAAAATATAAAGACA
TCTTGTACAGGAGGAAAATT

Downstream 100 bases:

>100_bases
AAGAGCCTGTCTTTCAGGTATATTTCTTTATTGGCTGTTTGTAATATGCTATAATCAAATTGAATAGAGACAATTTGAGG
TGAAATGCTGTATGAGTAAA

Product: MiaB family RNA modification protein

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNNRKYFILTYGCQMNVHDSEKLAGMLEEMGYKSTNNLEEADIIMVNTCAVRENAELRVFGRVGDFKRLKEKNPDLIIGV
GGCMMQIDENARKLYEKYPHVDLIFGTHNIHHIPELIKRIKEERGRIIEVWNQEEGLIPDIPYKREDDFKAWISIIQGCN
NFCTYCIVPYVRGRERSRPAADIISEARKLASEGVKEITLLGQNVNSYGKDLKEDIDFADLLKRLNRVEGIKRIRYMTSH
PRDFSDKMIKIIKECDKVCEHFHLPVQSGSTRILKKMNRGYTQAEYLNLIKKIKSQIPDYSITTDIIVGFPGETEEDFQE
TLKVIREVRFDMAYTFKYSPRKGTPAARHKDQVSEKIKQDRLTRLIEVQNSISLENNRKLKGKTVEVLIEGESRNNPDTF
EGRTRTNKLVIVPRNENLKGQIANVKINRVGSWTLYGEVIDNTRETVLNT

Sequences:

>Translated_450_residues
MNNRKYFILTYGCQMNVHDSEKLAGMLEEMGYKSTNNLEEADIIMVNTCAVRENAELRVFGRVGDFKRLKEKNPDLIIGV
GGCMMQIDENARKLYEKYPHVDLIFGTHNIHHIPELIKRIKEERGRIIEVWNQEEGLIPDIPYKREDDFKAWISIIQGCN
NFCTYCIVPYVRGRERSRPAADIISEARKLASEGVKEITLLGQNVNSYGKDLKEDIDFADLLKRLNRVEGIKRIRYMTSH
PRDFSDKMIKIIKECDKVCEHFHLPVQSGSTRILKKMNRGYTQAEYLNLIKKIKSQIPDYSITTDIIVGFPGETEEDFQE
TLKVIREVRFDMAYTFKYSPRKGTPAARHKDQVSEKIKQDRLTRLIEVQNSISLENNRKLKGKTVEVLIEGESRNNPDTF
EGRTRTNKLVIVPRNENLKGQIANVKINRVGSWTLYGEVIDNTRETVLNT
>Mature_450_residues
MNNRKYFILTYGCQMNVHDSEKLAGMLEEMGYKSTNNLEEADIIMVNTCAVRENAELRVFGRVGDFKRLKEKNPDLIIGV
GGCMMQIDENARKLYEKYPHVDLIFGTHNIHHIPELIKRIKEERGRIIEVWNQEEGLIPDIPYKREDDFKAWISIIQGCN
NFCTYCIVPYVRGRERSRPAADIISEARKLASEGVKEITLLGQNVNSYGKDLKEDIDFADLLKRLNRVEGIKRIRYMTSH
PRDFSDKMIKIIKECDKVCEHFHLPVQSGSTRILKKMNRGYTQAEYLNLIKKIKSQIPDYSITTDIIVGFPGETEEDFQE
TLKVIREVRFDMAYTFKYSPRKGTPAARHKDQVSEKIKQDRLTRLIEVQNSISLENNRKLKGKTVEVLIEGESRNNPDTF
EGRTRTNKLVIVPRNENLKGQIANVKINRVGSWTLYGEVIDNTRETVLNT

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=36.9294605809129, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=36.9294605809129, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=30.9734513274336, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=42.6303854875283, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=30.0438596491228, Blast_Score=201, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=34.5833333333333, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI71996771, Length=300, Percent_Identity=29, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21356207, Length=442, Percent_Identity=38.4615384615385, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=27.6688453159041, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52125; Mature: 52125

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRKYFILTYGCQMNVHDSEKLAGMLEEMGYKSTNNLEEADIIMVNTCAVRENAELRVF
CCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEE
GRVGDFKRLKEKNPDLIIGVGGCMMQIDENARKLYEKYPHVDLIFGTHNIHHIPELIKRI
ECCCCHHHHHCCCCCEEEECCCEEEEECHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHH
KEERGRIIEVWNQEEGLIPDIPYKREDDFKAWISIIQGCNNFCTYCIVPYVRGRERSRPA
HHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ADIISEARKLASEGVKEITLLGQNVNSYGKDLKEDIDFADLLKRLNRVEGIKRIRYMTSH
HHHHHHHHHHHHCCHHEEHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PRDFSDKMIKIIKECDKVCEHFHLPVQSGSTRILKKMNRGYTQAEYLNLIKKIKSQIPDY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
SITTDIIVGFPGETEEDFQETLKVIREVRFDMAYTFKYSPRKGTPAARHKDQVSEKIKQD
EEEEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RLTRLIEVQNSISLENNRKLKGKTVEVLIEGESRNNPDTFEGRTRTNKLVIVPRNENLKG
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCE
QIANVKINRVGSWTLYGEVIDNTRETVLNT
EEEEEEEECCCCEEEEHHHHHCCHHHHCCC
>Mature Secondary Structure
MNNRKYFILTYGCQMNVHDSEKLAGMLEEMGYKSTNNLEEADIIMVNTCAVRENAELRVF
CCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEE
GRVGDFKRLKEKNPDLIIGVGGCMMQIDENARKLYEKYPHVDLIFGTHNIHHIPELIKRI
ECCCCHHHHHCCCCCEEEECCCEEEEECHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHH
KEERGRIIEVWNQEEGLIPDIPYKREDDFKAWISIIQGCNNFCTYCIVPYVRGRERSRPA
HHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ADIISEARKLASEGVKEITLLGQNVNSYGKDLKEDIDFADLLKRLNRVEGIKRIRYMTSH
HHHHHHHHHHHHCCHHEEHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PRDFSDKMIKIIKECDKVCEHFHLPVQSGSTRILKKMNRGYTQAEYLNLIKKIKSQIPDY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
SITTDIIVGFPGETEEDFQETLKVIREVRFDMAYTFKYSPRKGTPAARHKDQVSEKIKQD
EEEEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RLTRLIEVQNSISLENNRKLKGKTVEVLIEGESRNNPDTFEGRTRTNKLVIVPRNENLKG
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCE
QIANVKINRVGSWTLYGEVIDNTRETVLNT
EEEEEEEECCCCEEEEHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]