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The map label for this gene is ribE [H]

Identifier: 220931697

GI number: 220931697

Start: 918529

End: 919197

Strand: Direct

Name: ribE [H]

Synonym: Hore_08540

Alternate gene names: 220931697

Gene position: 918529-919197 (Clockwise)

Preceding gene: 220931696

Following gene: 220931698

Centisome position: 35.63

GC content: 40.81

Gene sequence:

>669_bases
ATGTGTTTGTTCACCGGAATCGTCCAGGAAATCGGGGTACTCAGAGAAGTAGTCCGGACAGGAGGTACTTATCTTTTTTC
CATTACTGCCCGGAGGGTTTTGCAGGACATTAAGAAGGGAGATAGCATAGCCGTTAATGGGGTCTGCCTGACTGTAATAG
ATTTTAACGGAAATAGCTTTAATGCTGAACTTATGCCGGAAACATTAAAGGCCAGTAATCTGGGTGATTTAAAGCCCGGT
GCATTACTGAATCTGGAGCAGGCAGTGAGGGCAAAGGATTTTCTCGGAGGCCATATAGTGACCGGCCATGTTGATGGGAT
TGGAGTAGTTAAAAGTATCAGGAAAAATAGAAATGCTTATTTAGTGGAAATTGGGATCAAAAAGGACCTGACCAGATATC
TGGTGGAGAAAGGGTCCATAGCAGTAAACGGAGTCAGCCTGACCATAATGGAACTTTATTCTGATTCTTTCCTCATCTCC
CTGACCAGGGAAACCTGGAATTCTACTAATTTACATTGTGTAAAGACAGGGGATAGTTTGAATATAGAAACTGATTTGCT
TGGCAAATATGTCTACAAAATCCTGAAAAATTATTTCGGGAATAAAGAGTTTGTACCCCATCATACAACTATAAACAGGG
AATTTTTGATTGAAAATGGTTTTATCTGA

Upstream 100 bases:

>100_bases
GAGGGTGTAGCTGATATTGAAAAAGCATGGAAATTAAGGGATGTCCAATATAATTTTTTTGATGATAATATTTTAATCTC
AGCCTATTTATAGGGAGGTA

Downstream 100 bases:

>100_bases
AGGGGGTTTTAAGGGTGGATAAGATTGAGGGAGCCCTGGAAGAGCTTAAAGAGGGGAAAATGGTCATAGTAGTTGATGAT
GAAGACAGGGAGAATGAAGG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MCLFTGIVQEIGVLREVVRTGGTYLFSITARRVLQDIKKGDSIAVNGVCLTVIDFNGNSFNAELMPETLKASNLGDLKPG
ALLNLEQAVRAKDFLGGHIVTGHVDGIGVVKSIRKNRNAYLVEIGIKKDLTRYLVEKGSIAVNGVSLTIMELYSDSFLIS
LTRETWNSTNLHCVKTGDSLNIETDLLGKYVYKILKNYFGNKEFVPHHTTINREFLIENGFI

Sequences:

>Translated_222_residues
MCLFTGIVQEIGVLREVVRTGGTYLFSITARRVLQDIKKGDSIAVNGVCLTVIDFNGNSFNAELMPETLKASNLGDLKPG
ALLNLEQAVRAKDFLGGHIVTGHVDGIGVVKSIRKNRNAYLVEIGIKKDLTRYLVEKGSIAVNGVSLTIMELYSDSFLIS
LTRETWNSTNLHCVKTGDSLNIETDLLGKYVYKILKNYFGNKEFVPHHTTINREFLIENGFI
>Mature_222_residues
MCLFTGIVQEIGVLREVVRTGGTYLFSITARRVLQDIKKGDSIAVNGVCLTVIDFNGNSFNAELMPETLKASNLGDLKPG
ALLNLEQAVRAKDFLGGHIVTGHVDGIGVVKSIRKNRNAYLVEIGIKKDLTRYLVEKGSIAVNGVSLTIMELYSDSFLIS
LTRETWNSTNLHCVKTGDSLNIETDLLGKYVYKILKNYFGNKEFVPHHTTINREFLIENGFI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=204, Percent_Identity=33.8235294117647, Blast_Score=110, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319733, Length=204, Percent_Identity=39.7058823529412, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 24598; Mature: 24598

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLFTGIVQEIGVLREVVRTGGTYLFSITARRVLQDIKKGDSIAVNGVCLTVIDFNGNSF
CCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCEEECCEEEEEEEECCCEE
NAELMPETLKASNLGDLKPGALLNLEQAVRAKDFLGGHIVTGHVDGIGVVKSIRKNRNAY
CCEECCHHHCCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEE
LVEIGIKKDLTRYLVEKGSIAVNGVSLTIMELYSDSFLISLTRETWNSTNLHCVKTGDSL
EEEECCHHHHHHHHHHCCCEEECCCEEEEEEHHCCCEEEEEECCCCCCCCEEEEECCCCC
NIETDLLGKYVYKILKNYFGNKEFVPHHTTINREFLIENGFI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MCLFTGIVQEIGVLREVVRTGGTYLFSITARRVLQDIKKGDSIAVNGVCLTVIDFNGNSF
CCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCEEECCEEEEEEEECCCEE
NAELMPETLKASNLGDLKPGALLNLEQAVRAKDFLGGHIVTGHVDGIGVVKSIRKNRNAY
CCEECCHHHCCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEE
LVEIGIKKDLTRYLVEKGSIAVNGVSLTIMELYSDSFLISLTRETWNSTNLHCVKTGDSL
EEEECCHHHHHHHHHHCCCEEECCCEEEEEEHHCCCEEEEEECCCCCCCCEEEEECCCCC
NIETDLLGKYVYKILKNYFGNKEFVPHHTTINREFLIENGFI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2106516; 7934829; 9384377; 7473709 [H]