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The map label for this gene is 220931684

Identifier: 220931684

GI number: 220931684

Start: 903648

End: 904979

Strand: Direct

Name: 220931684

Synonym: Hore_08400

Alternate gene names: NA

Gene position: 903648-904979 (Clockwise)

Preceding gene: 220931683

Following gene: 220931685

Centisome position: 35.05

GC content: 34.53

Gene sequence:

>1332_bases
GTGAGATATAAAGAACAGGTAAAGTCATTAGTTGATATTTTTAAGTCTGGGGAAAAATCTCCTAATAATTTTAAATTAGG
TCTTGAGTTAGAGCATTTTATCCTTAATAAAGACGACCTTACCGCTGTATCCTATTATGAAGATAAAGGGGTAGAGGATA
TATTAAAAGAGCTGGTCAGGGTAAACTGGGAACCAGAATATGAGGGAGCTTACCTTATAAAACTTATAAAGGACAAAATG
ACTATTACTCTGGAACCGGGGGGGCAGCTGGAATTAAGCCTTGCCCCCGTGAGCACTATTAAAGAAATTGATTTACTTTA
TCAATCTTTTCTTGATGATATTTTCCCTATTTTACTTAAATGGGATAAAGTTATTGTTAATTTAGGCTACCATCCCGAAA
GTGGGATTAAGAATATTCCCCTGTTACCAAAAAACCGTTATAAATATATGTATGAGTATTTTAAAAATCGGGGAAGGTAT
GCCCATAATATGATGAAGGGAACAGCCTCAATCCAGGTAAGTCTGGATTATCAAGATGAGGATGATTATAGAAAGAAAAT
TATTTCTTCTTATTATTTGTCCCCAATAATCTATTGTATTTTTGACAATGGACCCTTTTTTGAAGGGAAATTATGGGATG
ATCCGGCAAGCATCAGGTCGATAATCTGGGATAACTGTGATAATGACAGGTGTGGTCTAATAAAAGGTATTTTTAAAGAT
GAATTTGGATATAAAGACTATGCATCCTATATTTTAAACTGTCCTCCAATAATTATAAAAAAAGATGGTAATCTTGTTTT
TACAGGTGACACCAGCGCCCGGGAACTATTAAGTTCATTTATTTCATCTAAAATTTCTAAAGAAGAATTAATGCATTTAT
TAACAATGGTCTTTCCTGACGTGCGCACCAAAAAATATCTCGAAATAAGAATAGGAGATTCTTTACCATACCCGTATTTT
TTGGGCTTCGTTGCCCTGTGGAAGGGTTTATTATATAATAAAGAAAACCTGGATTATCTTTACAGTAAAGCAGGGCAGGT
TGATAAAGGCATCTATTTTGAGTATAAAAACAGGATTCAAAGACATGGCTATAAAGCTTTCATTGACGGGGAGTCGGTAA
TCTCCAGGTTCAGGAAACTTAAGGGGATGGCTTCCATTGGTTTAACCTCAAGTGAGAGGAAGTATCTTGATTCCCTGGAT
TATATTATTGAAAATGGACCTACCCCTAAAGAAATTACATATCATAATTTGAAAATAGGTAAAAAAGAAGCTTTACAATG
GTGTATGGTTCCGGGAAAGGATGATTTTGTTGCAAGCACTGGATTTATTTAA

Upstream 100 bases:

>100_bases
TTAAACAACATAAAATATATTACACCGAAGCATACATGGGGTTAATTTATTATCATTTGTAAATACACTAAAATTATTTG
AAGAGAAAGGGTCTATAACA

Downstream 100 bases:

>100_bases
TAAATATAAAAAGATCATAACTTCAAATTCTAAAGAATATTACAGGGACTTCTTAAGGGCTAAAAAAAAGGTGGAAAAGT
CTACTGCATGGTATCATGGT

Product: Glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GshA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRYKEQVKSLVDIFKSGEKSPNNFKLGLELEHFILNKDDLTAVSYYEDKGVEDILKELVRVNWEPEYEGAYLIKLIKDKM
TITLEPGGQLELSLAPVSTIKEIDLLYQSFLDDIFPILLKWDKVIVNLGYHPESGIKNIPLLPKNRYKYMYEYFKNRGRY
AHNMMKGTASIQVSLDYQDEDDYRKKIISSYYLSPIIYCIFDNGPFFEGKLWDDPASIRSIIWDNCDNDRCGLIKGIFKD
EFGYKDYASYILNCPPIIIKKDGNLVFTGDTSARELLSSFISSKISKEELMHLLTMVFPDVRTKKYLEIRIGDSLPYPYF
LGFVALWKGLLYNKENLDYLYSKAGQVDKGIYFEYKNRIQRHGYKAFIDGESVISRFRKLKGMASIGLTSSERKYLDSLD
YIIENGPTPKEITYHNLKIGKKEALQWCMVPGKDDFVASTGFI

Sequences:

>Translated_443_residues
MRYKEQVKSLVDIFKSGEKSPNNFKLGLELEHFILNKDDLTAVSYYEDKGVEDILKELVRVNWEPEYEGAYLIKLIKDKM
TITLEPGGQLELSLAPVSTIKEIDLLYQSFLDDIFPILLKWDKVIVNLGYHPESGIKNIPLLPKNRYKYMYEYFKNRGRY
AHNMMKGTASIQVSLDYQDEDDYRKKIISSYYLSPIIYCIFDNGPFFEGKLWDDPASIRSIIWDNCDNDRCGLIKGIFKD
EFGYKDYASYILNCPPIIIKKDGNLVFTGDTSARELLSSFISSKISKEELMHLLTMVFPDVRTKKYLEIRIGDSLPYPYF
LGFVALWKGLLYNKENLDYLYSKAGQVDKGIYFEYKNRIQRHGYKAFIDGESVISRFRKLKGMASIGLTSSERKYLDSLD
YIIENGPTPKEITYHNLKIGKKEALQWCMVPGKDDFVASTGFI
>Mature_443_residues
MRYKEQVKSLVDIFKSGEKSPNNFKLGLELEHFILNKDDLTAVSYYEDKGVEDILKELVRVNWEPEYEGAYLIKLIKDKM
TITLEPGGQLELSLAPVSTIKEIDLLYQSFLDDIFPILLKWDKVIVNLGYHPESGIKNIPLLPKNRYKYMYEYFKNRGRY
AHNMMKGTASIQVSLDYQDEDDYRKKIISSYYLSPIIYCIFDNGPFFEGKLWDDPASIRSIIWDNCDNDRCGLIKGIFKD
EFGYKDYASYILNCPPIIIKKDGNLVFTGDTSARELLSSFISSKISKEELMHLLTMVFPDVRTKKYLEIRIGDSLPYPYF
LGFVALWKGLLYNKENLDYLYSKAGQVDKGIYFEYKNRIQRHGYKAFIDGESVISRFRKLKGMASIGLTSSERKYLDSLD
YIIENGPTPKEITYHNLKIGKKEALQWCMVPGKDDFVASTGFI

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51391; Mature: 51391

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKEQVKSLVDIFKSGEKSPNNFKLGLELEHFILNKDDLTAVSYYEDKGVEDILKELVR
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEHEEEECCCCCEEEEEHHCCCHHHHHHHHHH
VNWEPEYEGAYLIKLIKDKMTITLEPGGQLELSLAPVSTIKEIDLLYQSFLDDIFPILLK
CCCCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
WDKVIVNLGYHPESGIKNIPLLPKNRYKYMYEYFKNRGRYAHNMMKGTASIQVSLDYQDE
HHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEECCCC
DDYRKKIISSYYLSPIIYCIFDNGPFFEGKLWDDPASIRSIIWDNCDNDRCGLIKGIFKD
HHHHHHHHHHHHHCCEEEEEECCCCEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
EFGYKDYASYILNCPPIIIKKDGNLVFTGDTSARELLSSFISSKISKEELMHLLTMVFPD
CCCCHHHHHHHHCCCCEEEECCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VRTKKYLEIRIGDSLPYPYFLGFVALWKGLLYNKENLDYLYSKAGQVDKGIYFEYKNRIQ
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHH
RHGYKAFIDGESVISRFRKLKGMASIGLTSSERKYLDSLDYIIENGPTPKEITYHNLKIG
HCCCEEEECCHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
KKEALQWCMVPGKDDFVASTGFI
HHHHHHEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRYKEQVKSLVDIFKSGEKSPNNFKLGLELEHFILNKDDLTAVSYYEDKGVEDILKELVR
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEHEEEECCCCCEEEEEHHCCCHHHHHHHHHH
VNWEPEYEGAYLIKLIKDKMTITLEPGGQLELSLAPVSTIKEIDLLYQSFLDDIFPILLK
CCCCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
WDKVIVNLGYHPESGIKNIPLLPKNRYKYMYEYFKNRGRYAHNMMKGTASIQVSLDYQDE
HHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEECCCC
DDYRKKIISSYYLSPIIYCIFDNGPFFEGKLWDDPASIRSIIWDNCDNDRCGLIKGIFKD
HHHHHHHHHHHHHCCEEEEEECCCCEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
EFGYKDYASYILNCPPIIIKKDGNLVFTGDTSARELLSSFISSKISKEELMHLLTMVFPD
CCCCHHHHHHHHCCCCEEEECCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VRTKKYLEIRIGDSLPYPYFLGFVALWKGLLYNKENLDYLYSKAGQVDKGIYFEYKNRIQ
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHH
RHGYKAFIDGESVISRFRKLKGMASIGLTSSERKYLDSLDYIIENGPTPKEITYHNLKIG
HCCCEEEECCHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
KKEALQWCMVPGKDDFVASTGFI
HHHHHHEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA