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The map label for this gene is dut [H]

Identifier: 220931641

GI number: 220931641

Start: 857790

End: 858221

Strand: Direct

Name: dut [H]

Synonym: Hore_07970

Alternate gene names: 220931641

Gene position: 857790-858221 (Clockwise)

Preceding gene: 220931640

Following gene: 220931642

Centisome position: 33.27

GC content: 38.66

Gene sequence:

>432_bases
ATGAAAATCAAAGTTAAAAGATTGGACAAAACTTTACCTTTACCTGAATACCAGCATTATGGTGAAGATGCTGGTATGGA
TTTATATTCTGCTGAAGATAAAGTCCTTAAGCCCGGTGAATACAAATTAATAAAAACAGGCATAAAAATTGCTATACCAG
AAGGGTATGGAGGTTTTGTTTATCCGCGAAGTGGATTGGCATTAAAACATGGGATTACAGTGTTAAATGCAGATGGTGTT
ATTGATCCCGGTTATCGGGGAGAGGTTGGGGTAATCCTGATTAATCATAGTTCGTGTTCTTTTGAAATTAAGAAAGGGGA
CCGGATTGCCCAGTTGATAATACATAAAACCTATGAAATTGAGTGGGAAGAAGTCGAGGACCTGGATAATAGCACCAGGG
GAACTGGAGGTTTTGGTCATACGGGTATTTAG

Upstream 100 bases:

>100_bases
ATTCCTGTGCGTGTTCATTAAAAAATGTATCCCAATTGATAATTAATATCAATAGTTATAAAATATATAGTAATTATAAA
TAAAATTGAGGTGATACTGG

Downstream 100 bases:

>100_bases
CTGGAGTGGTTGTAATGAAATTAAGAGACCTTAGTGGAAAAAAGTTAATAAACATTAAAACCGGTCGGGTCCTTGGTACA
GTCAGGGATACTGATCTGGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MKIKVKRLDKTLPLPEYQHYGEDAGMDLYSAEDKVLKPGEYKLIKTGIKIAIPEGYGGFVYPRSGLALKHGITVLNADGV
IDPGYRGEVGVILINHSSCSFEIKKGDRIAQLIIHKTYEIEWEEVEDLDNSTRGTGGFGHTGI

Sequences:

>Translated_143_residues
MKIKVKRLDKTLPLPEYQHYGEDAGMDLYSAEDKVLKPGEYKLIKTGIKIAIPEGYGGFVYPRSGLALKHGITVLNADGV
IDPGYRGEVGVILINHSSCSFEIKKGDRIAQLIIHKTYEIEWEEVEDLDNSTRGTGGFGHTGI
>Mature_143_residues
MKIKVKRLDKTLPLPEYQHYGEDAGMDLYSAEDKVLKPGEYKLIKTGIKIAIPEGYGGFVYPRSGLALKHGITVLNADGV
IDPGYRGEVGVILINHSSCSFEIKKGDRIAQLIIHKTYEIEWEEVEDLDNSTRGTGGFGHTGI

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=142, Percent_Identity=50, Blast_Score=125, Evalue=9e-30,
Organism=Homo sapiens, GI4503423, Length=142, Percent_Identity=50, Blast_Score=125, Evalue=1e-29,
Organism=Homo sapiens, GI70906441, Length=142, Percent_Identity=50, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=43.2432432432432, Blast_Score=103, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71988561, Length=142, Percent_Identity=47.1830985915493, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319729, Length=143, Percent_Identity=40.5594405594406, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921126, Length=120, Percent_Identity=45.8333333333333, Blast_Score=105, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24583610, Length=120, Percent_Identity=45.8333333333333, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 15805; Mature: 15805

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKVKRLDKTLPLPEYQHYGEDAGMDLYSAEDKVLKPGEYKLIKTGIKIAIPEGYGGFV
CEEEEECCCCCCCCCCHHHCCCCCCCEEECCCCCEECCCCEEEEECCEEEEECCCCCCEE
YPRSGLALKHGITVLNADGVIDPGYRGEVGVILINHSSCSFEIKKGDRIAQLIIHKTYEI
ECCCCCEEECCEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCEEEEEEECCEEC
EWEEVEDLDNSTRGTGGFGHTGI
CHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIKVKRLDKTLPLPEYQHYGEDAGMDLYSAEDKVLKPGEYKLIKTGIKIAIPEGYGGFV
CEEEEECCCCCCCCCCHHHCCCCCCCEEECCCCCEECCCCEEEEECCEEEEECCCCCCEE
YPRSGLALKHGITVLNADGVIDPGYRGEVGVILINHSSCSFEIKKGDRIAQLIIHKTYEI
ECCCCCEEECCEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCEEEEEEECCEEC
EWEEVEDLDNSTRGTGGFGHTGI
CHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]