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The map label for this gene is infB

Identifier: 220931628

GI number: 220931628

Start: 842211

End: 844271

Strand: Direct

Name: infB

Synonym: Hore_07840

Alternate gene names: 220931628

Gene position: 842211-844271 (Clockwise)

Preceding gene: 220931627

Following gene: 220931629

Centisome position: 32.67

GC content: 39.69

Gene sequence:

>2061_bases
ATGAGTAAAATTAGAGTTTATAAACTGGCAAAGGAATTAGGTAAATCCAGTAAAGAACTGGTTAATATTTTAAATGATTT
AGGTGTTGAAGTATCCAGTCATATGAGTACAGTAGAGGATGAGACGGCTGAACTTGTTAAGGGAATGTTACAGGAAGAAG
ATAAACCTGAAGAAAAAATTAGTAAAAAAGAGCCAGATAAGAAAGATAGAAAGAAGACTAAAGGAAAAAAACAGATGACC
AGAACAGCCACTCAAAAAGAAGGTACAGAACATGGTCAGAAAGATACAGATAGAAGAGAGATGAGGGTAACTGTAAACCC
TCCCCTGTCTGTAAAAGAGCTGGCTGAGAAGGCTGATATTCCTGTTAATCGTATAATTAAAACATTAATTGGACTGGGGG
TTATGGCTACAGTTAATCATCAGATTGACGAAGAAATAGTTAAAAAACTAATAGACAAAATGAACTTAAAAATAAAAATT
TCTCAAGGGGAAGATAAAGAAGAAAAACCGGATAAAGTTCAGACTGATATAAAAGATAAACCTGAAGATTTAGAACTTCG
CCCTCCTATTGTCACTGTAATGGGACATGTTGATCATGGAAAAACAACCCTTCTTGATGTAATCAGGGAAACCCGGGTTG
CTGAAAGTGAGGCAGGAGGAATTACCCAGCATATAGGAGCTTATCAGGCAGTTGTACAGAATAAGAAGATTACATTTATA
GATACCCCCGGTCATGAGGCTTTTACTGCAATGAGGGCCAGGGGAGCCAGGTTGACCGATATTGCTATTTTAGTTGTTGC
AGCTGATGATGGGGTTATGCCCCAGACTGTAGAAGCTATAAATCATGCCAAGGCCGCTGATATCCCGATAATTGTTGCTA
TAAATAAGGTGGATAAATCCAATGCCCAGCCTGATATGGTAAAACAGCAGTTAACAGAGCATGGTCTGGTACCCGAAGAC
TGGGGTGGAGATACTATCTGTGTTCCCATTTCTGCTTTAAAGAAAAAGAATATAGATGAATTACTTGAAATGGTTCTTCT
TGTAGCTGAAATGGAAGAACTTAAGGCAAATCCCGATAGACCAGCAGAAGGTGTTATTGTAGAATCTCAGCTGGATAAAG
GACGGGGCCCGGTTGCCACAGTACTTGTAAAAAATGGAACCTTAAAGGTCGGTGATCCAATCCTGGCCGGTTATACTCAT
GGTAAGGTCAGGGCCATGATAAATGATCAGGGAAAACGTATTAAAGAGGCACTGCCGTCGACACCGGTTGAAGTTCTGGG
CTTTTCTGATGTGCCGGCAGCTGGTGATTATGTTCAGGTTCTCGAAGATGAAAAAGAGGCCCGGGCTATTGCAGAAGAAA
GATTACAGAAAAAGCAGGAACGTGATTTACAGCATGACGGGCGTATTTCCCTCGATGGTTTATATCAACAGATAAAAGAG
GGAGGAGTTAAGGAGCTTAATTTAATAATCAAGGGAGATGTTCATGGTTCTATTGAGGCGTTACGTGAATCCCTTGTAAA
ATTAAGTACTGATGAGGTTACGGTCAATATTATTCATACAGGGGTCGGTGCTATTAACGAAACAGATGTTAACCTGGCCA
GTGCTTCAAATGCAATTATAATTGGTTTTAATGTCAGGCCTGATTCCAATGCCAGAAAATTGGCTGAACGGGAAAAAGTT
GAGATAAAAACCTACCGGGTTATTTACAAGATAATTGAAGACCTTAAAGATGCTATGGCCGGTATGCTTGAACCTGAACT
TAAAGAAGAAGTTACCGGAAGGGCTGAAGTCAGAGCTACATTTAAGGTGCCCAATGTTGGAACCGTAGCCGGGCTTTATG
TTAAAGAAGGGTTTATCAATCGAAATAATAAAGTGCGTTTATTGAGGGATGGCGTAGTCGTCTATGAAGGTGATATTGCT
TCCCTGAAAAGATTTAAAAATGATGTCAGGGAAGTTAAAGAAGGTTATGAATGTGGTCTGGGAATTGAAGGTTATAATGA
TATTAAAGAAGGGGACCAGATTGAAACCTATACCTATAGGGAAATAAAACGCACCTTATAG

Upstream 100 bases:

>100_bases
ATCTCTAAAGACTTCAATTTCTGACGAGATTTATACAAAATTGATGGAGGAGATTGATAAAATGAATGGATAGTCAGGTA
ATTTTTCTGGAGGTGAAATT

Downstream 100 bases:

>100_bases
GTTTTAAGAGGTGAGATAAATGAAGAGGCAACGAGCCCGAAGACTTGGTGAACTTTTAAAAAAAGAAATTAGCGATATTA
TACTGAGAGAAGTTAAAGAC

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MSKIRVYKLAKELGKSSKELVNILNDLGVEVSSHMSTVEDETAELVKGMLQEEDKPEEKISKKEPDKKDRKKTKGKKQMT
RTATQKEGTEHGQKDTDRREMRVTVNPPLSVKELAEKADIPVNRIIKTLIGLGVMATVNHQIDEEIVKKLIDKMNLKIKI
SQGEDKEEKPDKVQTDIKDKPEDLELRPPIVTVMGHVDHGKTTLLDVIRETRVAESEAGGITQHIGAYQAVVQNKKITFI
DTPGHEAFTAMRARGARLTDIAILVVAADDGVMPQTVEAINHAKAADIPIIVAINKVDKSNAQPDMVKQQLTEHGLVPED
WGGDTICVPISALKKKNIDELLEMVLLVAEMEELKANPDRPAEGVIVESQLDKGRGPVATVLVKNGTLKVGDPILAGYTH
GKVRAMINDQGKRIKEALPSTPVEVLGFSDVPAAGDYVQVLEDEKEARAIAEERLQKKQERDLQHDGRISLDGLYQQIKE
GGVKELNLIIKGDVHGSIEALRESLVKLSTDEVTVNIIHTGVGAINETDVNLASASNAIIIGFNVRPDSNARKLAEREKV
EIKTYRVIYKIIEDLKDAMAGMLEPELKEEVTGRAEVRATFKVPNVGTVAGLYVKEGFINRNNKVRLLRDGVVVYEGDIA
SLKRFKNDVREVKEGYECGLGIEGYNDIKEGDQIETYTYREIKRTL

Sequences:

>Translated_686_residues
MSKIRVYKLAKELGKSSKELVNILNDLGVEVSSHMSTVEDETAELVKGMLQEEDKPEEKISKKEPDKKDRKKTKGKKQMT
RTATQKEGTEHGQKDTDRREMRVTVNPPLSVKELAEKADIPVNRIIKTLIGLGVMATVNHQIDEEIVKKLIDKMNLKIKI
SQGEDKEEKPDKVQTDIKDKPEDLELRPPIVTVMGHVDHGKTTLLDVIRETRVAESEAGGITQHIGAYQAVVQNKKITFI
DTPGHEAFTAMRARGARLTDIAILVVAADDGVMPQTVEAINHAKAADIPIIVAINKVDKSNAQPDMVKQQLTEHGLVPED
WGGDTICVPISALKKKNIDELLEMVLLVAEMEELKANPDRPAEGVIVESQLDKGRGPVATVLVKNGTLKVGDPILAGYTH
GKVRAMINDQGKRIKEALPSTPVEVLGFSDVPAAGDYVQVLEDEKEARAIAEERLQKKQERDLQHDGRISLDGLYQQIKE
GGVKELNLIIKGDVHGSIEALRESLVKLSTDEVTVNIIHTGVGAINETDVNLASASNAIIIGFNVRPDSNARKLAEREKV
EIKTYRVIYKIIEDLKDAMAGMLEPELKEEVTGRAEVRATFKVPNVGTVAGLYVKEGFINRNNKVRLLRDGVVVYEGDIA
SLKRFKNDVREVKEGYECGLGIEGYNDIKEGDQIETYTYREIKRTL
>Mature_685_residues
SKIRVYKLAKELGKSSKELVNILNDLGVEVSSHMSTVEDETAELVKGMLQEEDKPEEKISKKEPDKKDRKKTKGKKQMTR
TATQKEGTEHGQKDTDRREMRVTVNPPLSVKELAEKADIPVNRIIKTLIGLGVMATVNHQIDEEIVKKLIDKMNLKIKIS
QGEDKEEKPDKVQTDIKDKPEDLELRPPIVTVMGHVDHGKTTLLDVIRETRVAESEAGGITQHIGAYQAVVQNKKITFID
TPGHEAFTAMRARGARLTDIAILVVAADDGVMPQTVEAINHAKAADIPIIVAINKVDKSNAQPDMVKQQLTEHGLVPEDW
GGDTICVPISALKKKNIDELLEMVLLVAEMEELKANPDRPAEGVIVESQLDKGRGPVATVLVKNGTLKVGDPILAGYTHG
KVRAMINDQGKRIKEALPSTPVEVLGFSDVPAAGDYVQVLEDEKEARAIAEERLQKKQERDLQHDGRISLDGLYQQIKEG
GVKELNLIIKGDVHGSIEALRESLVKLSTDEVTVNIIHTGVGAINETDVNLASASNAIIIGFNVRPDSNARKLAEREKVE
IKTYRVIYKIIEDLKDAMAGMLEPELKEEVTGRAEVRATFKVPNVGTVAGLYVKEGFINRNNKVRLLRDGVVVYEGDIAS
LKRFKNDVREVKEGYECGLGIEGYNDIKEGDQIETYTYREIKRTL

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=653, Percent_Identity=38.7442572741194, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI53729337, Length=653, Percent_Identity=38.7442572741194, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI84043963, Length=468, Percent_Identity=29.4871794871795, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI157426893, Length=229, Percent_Identity=27.9475982532751, Blast_Score=87, Evalue=6e-17,
Organism=Escherichia coli, GI1789559, Length=658, Percent_Identity=48.7841945288754, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI2367247, Length=213, Percent_Identity=35.2112676056338, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788922, Length=228, Percent_Identity=30.2631578947368, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1790412, Length=242, Percent_Identity=32.6446280991736, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789737, Length=242, Percent_Identity=32.6446280991736, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI48994988, Length=139, Percent_Identity=34.5323741007194, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=560, Percent_Identity=37.3214285714286, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI212656558, Length=566, Percent_Identity=27.208480565371, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17557151, Length=227, Percent_Identity=32.5991189427313, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=247, Percent_Identity=33.1983805668016, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=239, Percent_Identity=30.9623430962343, Blast_Score=66, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=623, Percent_Identity=37.3996789727127, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319282, Length=474, Percent_Identity=28.4810126582279, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=230, Percent_Identity=28.695652173913, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=235, Percent_Identity=32.7659574468085, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28572034, Length=578, Percent_Identity=41.6955017301038, Blast_Score=401, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24656849, Length=472, Percent_Identity=27.9661016949153, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI160714833, Length=116, Percent_Identity=53.448275862069, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI78706572, Length=218, Percent_Identity=29.3577981651376, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281363316, Length=228, Percent_Identity=32.8947368421053, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17864358, Length=228, Percent_Identity=32.8947368421053, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_HALOH (B8CW72)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508536.1
- ProteinModelPortal:   B8CW72
- SMR:   B8CW72
- GeneID:   7314771
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_07840
- HOGENOM:   HBG635083
- OMA:   QYGRIRS
- ProtClustDB:   CLSK2806299
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR009061
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF46955 Putativ_DNA_bind; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 75988; Mature: 75857

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIRVYKLAKELGKSSKELVNILNDLGVEVSSHMSTVEDETAELVKGMLQEEDKPEEKI
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
SKKEPDKKDRKKTKGKKQMTRTATQKEGTEHGQKDTDRREMRVTVNPPLSVKELAEKADI
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCC
PVNRIIKTLIGLGVMATVNHQIDEEIVKKLIDKMNLKIKISQGEDKEEKPDKVQTDIKDK
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHCCCCCC
PEDLELRPPIVTVMGHVDHGKTTLLDVIRETRVAESEAGGITQHIGAYQAVVQNKKITFI
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEE
DTPGHEAFTAMRARGARLTDIAILVVAADDGVMPQTVEAINHAKAADIPIIVAINKVDKS
ECCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
NAQPDMVKQQLTEHGLVPEDWGGDTICVPISALKKKNIDELLEMVLLVAEMEELKANPDR
CCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
PAEGVIVESQLDKGRGPVATVLVKNGTLKVGDPILAGYTHGKVRAMINDQGKRIKEALPS
CCCCEEEECCCCCCCCCEEEEEEECCEEEECCCEEECCCCCEEEEEECHHHHHHHHHCCC
TPVEVLGFSDVPAAGDYVQVLEDEKEARAIAEERLQKKQERDLQHDGRISLDGLYQQIKE
CCHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
GGVKELNLIIKGDVHGSIEALRESLVKLSTDEVTVNIIHTGVGAINETDVNLASASNAII
CCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCEEE
IGFNVRPDSNARKLAEREKVEIKTYRVIYKIIEDLKDAMAGMLEPELKEEVTGRAEVRAT
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEE
FKVPNVGTVAGLYVKEGFINRNNKVRLLRDGVVVYEGDIASLKRFKNDVREVKEGYECGL
EECCCCCHHHHHHHHHCCCCCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
GIEGYNDIKEGDQIETYTYREIKRTL
CCCCCCCCCCCCCEEHHHHHHHHHCC
>Mature Secondary Structure 
SKIRVYKLAKELGKSSKELVNILNDLGVEVSSHMSTVEDETAELVKGMLQEEDKPEEKI
CCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
SKKEPDKKDRKKTKGKKQMTRTATQKEGTEHGQKDTDRREMRVTVNPPLSVKELAEKADI
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCC
PVNRIIKTLIGLGVMATVNHQIDEEIVKKLIDKMNLKIKISQGEDKEEKPDKVQTDIKDK
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHCCCCCC
PEDLELRPPIVTVMGHVDHGKTTLLDVIRETRVAESEAGGITQHIGAYQAVVQNKKITFI
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEE
DTPGHEAFTAMRARGARLTDIAILVVAADDGVMPQTVEAINHAKAADIPIIVAINKVDKS
ECCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
NAQPDMVKQQLTEHGLVPEDWGGDTICVPISALKKKNIDELLEMVLLVAEMEELKANPDR
CCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
PAEGVIVESQLDKGRGPVATVLVKNGTLKVGDPILAGYTHGKVRAMINDQGKRIKEALPS
CCCCEEEECCCCCCCCCEEEEEEECCEEEECCCEEECCCCCEEEEEECHHHHHHHHHCCC
TPVEVLGFSDVPAAGDYVQVLEDEKEARAIAEERLQKKQERDLQHDGRISLDGLYQQIKE
CCHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
GGVKELNLIIKGDVHGSIEALRESLVKLSTDEVTVNIIHTGVGAINETDVNLASASNAII
CCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCEEE
IGFNVRPDSNARKLAEREKVEIKTYRVIYKIIEDLKDAMAGMLEPELKEEVTGRAEVRAT
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEE
FKVPNVGTVAGLYVKEGFINRNNKVRLLRDGVVVYEGDIASLKRFKNDVREVKEGYECGL
EECCCCCHHHHHHHHHCCCCCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
GIEGYNDIKEGDQIETYTYREIKRTL
CCCCCCCCCCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA