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The map label for this gene is rasP [H]

Identifier: 220931618

GI number: 220931618

Start: 829859

End: 830932

Strand: Direct

Name: rasP [H]

Synonym: Hore_07740

Alternate gene names: 220931618

Gene position: 829859-830932 (Clockwise)

Preceding gene: 220931617

Following gene: 220931619

Centisome position: 32.19

GC content: 37.52

Gene sequence:

>1074_bases
ATGTTAACAACTATTATAAGCTTTATAATTGTTTTAGGAATATTAATCTTTATTCATGAGTTTGGTCATTATATTACTGC
CAAAAAAGCGGGTATCAGGGTTGAAGAATTTGCCCTTGGATATGGTCCCCGGCTTTTTTCCCGTCAAAAGGGAGAGACTG
TTTATTCTATCAGGGCTCTGCCTCTGGGGGGATTCTGTAAGATGACGGGAGAGTTTCCTGAAGATGAAGAAATGACCGAA
GAAGAACGAAAAATATATTTAGATGCCAAGGAGAAGGGGGAATGTTTTTTTCAGAAATCACCCATTAAGCGATTTGCTGT
AATTTTTATGGGCCCCTTTATGAACTTTATGCTGGCCGTATTACTTTTCATTTTAATGTTTTCAATTTATGGTATTCCTG
TTGATAGCTCCAGTACTACTATAATCGGTACGATAGTTCCTGAAAAACCGGCGGCAGAGGCCGGTCTTGAACCGGGGGAT
AAAATTATAGAAATAAACGGTACCAGGGTTGAAAACTGGGATGAGATGGCGTCAATTATACACCGTTCTCCTGGAAAAAA
GCTGGAGATTAAATACATACGAAACAATGAGATCAGAGAAGTGACCCTGATACCTGACTTTAATGAGAATACCGAAACAG
GGGTCATTGGGATTTATCCTGAATTGATTATGAAAAAAGTCAGTTTCACAAAATCTATAAAAATGGGGTTTTATCAAACC
TGGTATGTATTTTCAAATACGATAATGGCTTTTGTAAAAATTATAACCAGAGAGACTTCTGCCGAATTAGCCGGACCGAT
TATGATAGCAAATATGGTTGGTCAGGCAGCTAAAGTTGGACTTTTAAACCTCCTCAATTTAATGGCTGTAATCAGTATAA
ATCTTGGTATTCTTAATCTCCTGCCTTTTCCTGCCCTTGACGGAGGGAGGATTGTATTTATTCTGGTTGAGGTAGTCAGG
GGTAAACCGGTTGACCCTGAAAAAGAAGGATTTGTTCACTTGATTGGTTTTGTCTTATTGATGGTATTAATGGTTTTTGT
TATATATAAAGATATTATGAGAACATGGTTTTGA

Upstream 100 bases:

>100_bases
GGGTTATGGAAAACCATCAGATAATTTCTTCTCCGGACCTGGAAGATGTAATAGAGGTTGATAACTGGGCCCGGGCCAGG
GCTAAGGAGGTAACTGGACA

Downstream 100 bases:

>100_bases
ATGGTTTCACTGGAGGTGTAGTATTTGTATAAGGTTACCCTAGAATCTTTATTAACAGATAGTATTGCTTTAAAGCATTT
ACCCAACGCCGGATTTCGAC

Product: putative membrane-associated zinc metalloprotease

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLTTIISFIIVLGILIFIHEFGHYITAKKAGIRVEEFALGYGPRLFSRQKGETVYSIRALPLGGFCKMTGEFPEDEEMTE
EERKIYLDAKEKGECFFQKSPIKRFAVIFMGPFMNFMLAVLLFILMFSIYGIPVDSSSTTIIGTIVPEKPAAEAGLEPGD
KIIEINGTRVENWDEMASIIHRSPGKKLEIKYIRNNEIREVTLIPDFNENTETGVIGIYPELIMKKVSFTKSIKMGFYQT
WYVFSNTIMAFVKIITRETSAELAGPIMIANMVGQAAKVGLLNLLNLMAVISINLGILNLLPFPALDGGRIVFILVEVVR
GKPVDPEKEGFVHLIGFVLLMVLMVFVIYKDIMRTWF

Sequences:

>Translated_357_residues
MLTTIISFIIVLGILIFIHEFGHYITAKKAGIRVEEFALGYGPRLFSRQKGETVYSIRALPLGGFCKMTGEFPEDEEMTE
EERKIYLDAKEKGECFFQKSPIKRFAVIFMGPFMNFMLAVLLFILMFSIYGIPVDSSSTTIIGTIVPEKPAAEAGLEPGD
KIIEINGTRVENWDEMASIIHRSPGKKLEIKYIRNNEIREVTLIPDFNENTETGVIGIYPELIMKKVSFTKSIKMGFYQT
WYVFSNTIMAFVKIITRETSAELAGPIMIANMVGQAAKVGLLNLLNLMAVISINLGILNLLPFPALDGGRIVFILVEVVR
GKPVDPEKEGFVHLIGFVLLMVLMVFVIYKDIMRTWF
>Mature_357_residues
MLTTIISFIIVLGILIFIHEFGHYITAKKAGIRVEEFALGYGPRLFSRQKGETVYSIRALPLGGFCKMTGEFPEDEEMTE
EERKIYLDAKEKGECFFQKSPIKRFAVIFMGPFMNFMLAVLLFILMFSIYGIPVDSSSTTIIGTIVPEKPAAEAGLEPGD
KIIEINGTRVENWDEMASIIHRSPGKKLEIKYIRNNEIREVTLIPDFNENTETGVIGIYPELIMKKVSFTKSIKMGFYQT
WYVFSNTIMAFVKIITRETSAELAGPIMIANMVGQAAKVGLLNLLNLMAVISINLGILNLLPFPALDGGRIVFILVEVVR
GKPVDPEKEGFVHLIGFVLLMVLMVFVIYKDIMRTWF

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor [

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=225, Percent_Identity=31.1111111111111, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 40234; Mature: 40234

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTIISFIIVLGILIFIHEFGHYITAKKAGIRVEEFALGYGPRLFSRQKGETVYSIRAL
CHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCHHHHHCCCCCHHHCCCCCCEEEEEEEC
PLGGFCKMTGEFPEDEEMTEEERKIYLDAKEKGECFFQKSPIKRFAVIFMGPFMNFMLAV
CCCCCEEECCCCCCCHHHCHHHHEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LLFILMFSIYGIPVDSSSTTIIGTIVPEKPAAEAGLEPGDKIIEINGTRVENWDEMASII
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCCCCCCEEEEECCCCCCCHHHHHHHH
HRSPGKKLEIKYIRNNEIREVTLIPDFNENTETGVIGIYPELIMKKVSFTKSIKMGFYQT
HCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
WYVFSNTIMAFVKIITRETSAELAGPIMIANMVGQAAKVGLLNLLNLMAVISINLGILNL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LPFPALDGGRIVFILVEVVRGKPVDPEKEGFVHLIGFVLLMVLMVFVIYKDIMRTWF
CCCCCCCCCEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTTIISFIIVLGILIFIHEFGHYITAKKAGIRVEEFALGYGPRLFSRQKGETVYSIRAL
CHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCHHHHHCCCCCHHHCCCCCCEEEEEEEC
PLGGFCKMTGEFPEDEEMTEEERKIYLDAKEKGECFFQKSPIKRFAVIFMGPFMNFMLAV
CCCCCEEECCCCCCCHHHCHHHHEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LLFILMFSIYGIPVDSSSTTIIGTIVPEKPAAEAGLEPGDKIIEINGTRVENWDEMASII
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCCCCCCEEEEECCCCCCCHHHHHHHH
HRSPGKKLEIKYIRNNEIREVTLIPDFNENTETGVIGIYPELIMKKVSFTKSIKMGFYQT
HCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
WYVFSNTIMAFVKIITRETSAELAGPIMIANMVGQAAKVGLLNLLNLMAVISINLGILNL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LPFPALDGGRIVFILVEVVRGKPVDPEKEGFVHLIGFVLLMVLMVFVIYKDIMRTWF
CCCCCCCCCEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA