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The map label for this gene is trmFO [H]

Identifier: 220931599

GI number: 220931599

Start: 813115

End: 814428

Strand: Direct

Name: trmFO [H]

Synonym: Hore_07550

Alternate gene names: 220931599

Gene position: 813115-814428 (Clockwise)

Preceding gene: 220931598

Following gene: 220931600

Centisome position: 31.54

GC content: 40.03

Gene sequence:

>1314_bases
ATGGATAGATTGATAGTAATTGGAGCAGGACTCGCAGGCTGTGAGGCAACCTGGCAGGCGATTAACAGAGGGGTTGAGGT
CGATCTTTACGAAATGAGACCTGAACAAAAAACCCCGGCCCACAATACCGGTTTTTTTGCTGAACTTGTCTGTAGTAATT
CTCTGGGGGCAAACCACCTTCACAATGCCGGGGGTATTTTAAAAGAGGAAATGAGACATCTCAATTCCCTAATAATTAAA
ACTGCCGATAAACACAGGGTACCTGCTGGAAGGGCCCTTGCTGTTGATCGTAATGAGTTTGCCAGGGAAATAACTAAAAA
GCTAAAAGAACATCCCGGGGTACATTATCATAACGAAGAGGTAACTGATATTCCTGAAGATAGAGTTGTAATTATTGCCA
CCGGGCCTTTGACCTCAGATAAACTTACTAAAGAAATAAAAAAATTAACCGGTGAGGAATATCTTTATTTTTTTGATGCA
GCTGCACCTATTATAACAGCTGATAGTATTGATTATAATAAGGTTTTCAGGGCATCCCGTTATGAAGATGGAAAGGGAGA
TTATCTAAACTGTCCTATGTCCAGAGAGGAATATGAAGAATTCTGGAATGCCCTTGTTTCAGCCGAAAGACACCAGCCCC
ATAACTTTGAAAAAGATATGTATTTTGAGGGATGTCTACCCATAGAGGTTCTGGCAGAAAGGGGTAAAAAGACTTTGCTT
TTTGGCCCCCTGAAACCAGTTGGATTAGTTGATCCCAATACCCAGGAAAAACCTTATGCGGTAGTTCAATTAAGACAGGA
TAATGCAGAGGGTACCCTTTATAACCTGGTCGGTTTTCAGACCAGATTAAAATGGGGAGAACAGAAAAGAATAATAAATA
TGATACCCGGACTTGAAAATGCAGAAATAGTCAGGTATGGTGTAATGCATCGTAATACTTACCTGAATTCACCTAAATTA
CTGAATTCTTGCTACCAGTTAAGGCATAAGGAGAACATCTTTTTTGCTGGTCAAATAACCGGGGTTGAAGGTTATGTTGA
GTCTGCCTCATCTGGACTTATAGCCGGCTTAAATGCCGCCAGACTGTTACAGGGTAAAGAGTTATTAAGATTTCCCAGAA
CCACAGCTCATGGGGCTTTAAGTAGTTATATATCAAATCCGGCCAAAGATAAGCTGCAACCCATGAATATTAACTTTGGT
TTATTTCCTCCCCTTGATATAAAAATACGGAACAAAAGAGAGCGCAAGGAAAAAAAAGGAACTAGAGCCCTTGAAGATCT
TAAAGGTTTTATGTTAACATATGGTTTACATTAA

Upstream 100 bases:

>100_bases
CCTTATGGTAGAGAAGAACTCAAAAAAGCAAGGCCGGTATTATCTCTGTATTAACAAAGAGTGTGGTTATAAGAAAGAAG
TATAAAGAGGTGGAATATAT

Downstream 100 bases:

>100_bases
GTTAACATTATTTTAACAAGATTGTTTTAATATTTTGCTGGAGGGTTCTTGACACATAGGGGGGGTTATGTTAATATATA
AATTAGTGAATCAAAAAACA

Product: gid protein

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MDRLIVIGAGLAGCEATWQAINRGVEVDLYEMRPEQKTPAHNTGFFAELVCSNSLGANHLHNAGGILKEEMRHLNSLIIK
TADKHRVPAGRALAVDRNEFAREITKKLKEHPGVHYHNEEVTDIPEDRVVIIATGPLTSDKLTKEIKKLTGEEYLYFFDA
AAPIITADSIDYNKVFRASRYEDGKGDYLNCPMSREEYEEFWNALVSAERHQPHNFEKDMYFEGCLPIEVLAERGKKTLL
FGPLKPVGLVDPNTQEKPYAVVQLRQDNAEGTLYNLVGFQTRLKWGEQKRIINMIPGLENAEIVRYGVMHRNTYLNSPKL
LNSCYQLRHKENIFFAGQITGVEGYVESASSGLIAGLNAARLLQGKELLRFPRTTAHGALSSYISNPAKDKLQPMNINFG
LFPPLDIKIRNKRERKEKKGTRALEDLKGFMLTYGLH

Sequences:

>Translated_437_residues
MDRLIVIGAGLAGCEATWQAINRGVEVDLYEMRPEQKTPAHNTGFFAELVCSNSLGANHLHNAGGILKEEMRHLNSLIIK
TADKHRVPAGRALAVDRNEFAREITKKLKEHPGVHYHNEEVTDIPEDRVVIIATGPLTSDKLTKEIKKLTGEEYLYFFDA
AAPIITADSIDYNKVFRASRYEDGKGDYLNCPMSREEYEEFWNALVSAERHQPHNFEKDMYFEGCLPIEVLAERGKKTLL
FGPLKPVGLVDPNTQEKPYAVVQLRQDNAEGTLYNLVGFQTRLKWGEQKRIINMIPGLENAEIVRYGVMHRNTYLNSPKL
LNSCYQLRHKENIFFAGQITGVEGYVESASSGLIAGLNAARLLQGKELLRFPRTTAHGALSSYISNPAKDKLQPMNINFG
LFPPLDIKIRNKRERKEKKGTRALEDLKGFMLTYGLH
>Mature_437_residues
MDRLIVIGAGLAGCEATWQAINRGVEVDLYEMRPEQKTPAHNTGFFAELVCSNSLGANHLHNAGGILKEEMRHLNSLIIK
TADKHRVPAGRALAVDRNEFAREITKKLKEHPGVHYHNEEVTDIPEDRVVIIATGPLTSDKLTKEIKKLTGEEYLYFFDA
AAPIITADSIDYNKVFRASRYEDGKGDYLNCPMSREEYEEFWNALVSAERHQPHNFEKDMYFEGCLPIEVLAERGKKTLL
FGPLKPVGLVDPNTQEKPYAVVQLRQDNAEGTLYNLVGFQTRLKWGEQKRIINMIPGLENAEIVRYGVMHRNTYLNSPKL
LNSCYQLRHKENIFFAGQITGVEGYVESASSGLIAGLNAARLLQGKELLRFPRTTAHGALSSYISNPAKDKLQPMNINFG
LFPPLDIKIRNKRERKEKKGTRALEDLKGFMLTYGLH

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=100, Percent_Identity=40, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49383; Mature: 49383

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLIVIGAGLAGCEATWQAINRGVEVDLYEMRPEQKTPAHNTGFFAELVCSNSLGANHL
CCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCHH
HNAGGILKEEMRHLNSLIIKTADKHRVPAGRALAVDRNEFAREITKKLKEHPGVHYHNEE
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCEECCCC
VTDIPEDRVVIIATGPLTSDKLTKEIKKLTGEEYLYFFDAAAPIITADSIDYNKVFRASR
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHCC
YEDGKGDYLNCPMSREEYEEFWNALVSAERHQPHNFEKDMYFEGCLPIEVLAERGKKTLL
CCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHCCCEEEE
FGPLKPVGLVDPNTQEKPYAVVQLRQDNAEGTLYNLVGFQTRLKWGEQKRIINMIPGLEN
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCHHHHHHHCCCCCC
AEIVRYGVMHRNTYLNSPKLLNSCYQLRHKENIFFAGQITGVEGYVESASSGLIAGLNAA
CCEEEEEHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCEEECCCHH
RLLQGKELLRFPRTTAHGALSSYISNPAKDKLQPMNINFGLFPPLDIKIRNKRERKEKKG
HHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCEECCCCCCCCCCEEECCHHHHHHHHH
TRALEDLKGFMLTYGLH
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRLIVIGAGLAGCEATWQAINRGVEVDLYEMRPEQKTPAHNTGFFAELVCSNSLGANHL
CCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCHH
HNAGGILKEEMRHLNSLIIKTADKHRVPAGRALAVDRNEFAREITKKLKEHPGVHYHNEE
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCEECCCC
VTDIPEDRVVIIATGPLTSDKLTKEIKKLTGEEYLYFFDAAAPIITADSIDYNKVFRASR
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHCC
YEDGKGDYLNCPMSREEYEEFWNALVSAERHQPHNFEKDMYFEGCLPIEVLAERGKKTLL
CCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHCCCEEEE
FGPLKPVGLVDPNTQEKPYAVVQLRQDNAEGTLYNLVGFQTRLKWGEQKRIINMIPGLEN
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCHHHHHHHCCCCCC
AEIVRYGVMHRNTYLNSPKLLNSCYQLRHKENIFFAGQITGVEGYVESASSGLIAGLNAA
CCEEEEEHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCEEECCCHH
RLLQGKELLRFPRTTAHGALSSYISNPAKDKLQPMNINFGLFPPLDIKIRNKRERKEKKG
HHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCEECCCCCCCCCCEEECCHHHHHHHHH
TRALEDLKGFMLTYGLH
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA