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The map label for this gene is topA [H]

Identifier: 220931598

GI number: 220931598

Start: 811024

End: 813099

Strand: Direct

Name: topA [H]

Synonym: Hore_07540

Alternate gene names: 220931598

Gene position: 811024-813099 (Clockwise)

Preceding gene: 220931597

Following gene: 220931599

Centisome position: 31.46

GC content: 37.48

Gene sequence:

>2076_bases
ATGGGGAATAAAAGTAATAATACACTGGTAATTGTTGAATCTCCTGCTAAAGCTAAAACAATATCAAAGTTTCTGGGAAA
AGGATATAAAGTGGAAGCAACAATGGGCCATGTTATTGATCTACCCAAGAGCAAACTGGGAATAAATATAGATAAAGGTT
TTGAACCCAGGTATATAACAATCAGGGGTAAAGGAAAAGTATTAAAAAAATTAAGAAAGGAAGTAAAAAAGAGTAAAGAT
GTTCTCCTGGCAACTGACCCTGATAGAGAAGGGGAAGCCATATCATGGCATCTGACCCGGGCTTTGAAAATAGATGAAGA
TAAACCAAGGATTGAATTTAATGAAATAACTAAAAGTGCTATTAAAAATGCCCTTAAAAACCGCAGGCCTATAGATAAGA
ATCTGGTTAATTCCCAGCAGGCCAGACGCCTGCTTGACCGTCTTGTCGGTTACAAACTCAGTCCACTATTATGGAAAAAG
GTGAGACGGGGTTTAAGTGCCGGGCGTGTTCAGACAGTTGCTGTCAAATTGTTATGTAATCGGGAAAAAGAGATAGAGTC
TTTCGAACCTGAAGAATACTGGACTATTTCTGCTTCATTTAATAAAAAAGATAAAGATTTTATAGCTGATCTGTACCGAA
TTTCAGGTAAAAAATTCAAAATTAATAATGAGAAAGAAGCAAAACAAATTTTAGAAGATTTAAATAAAAGTAAATTTGTT
GTAAGTGACATAAAAGAAAAAACAAGGAAACGTAACCCCAATCCACCCTTTACAACAAGTACCCTTCAACAGAGGGCTTC
TTCGATTCTGGGCTTTTCTGCTAAAAAAACAATGTATCTGGCCCAGCAGTTATATGAAGGAATAGATATGGGTAGCGAAG
GGACCACTGGTCTTATAAGTTATATACGTACTGACAGTACAAGAATCTCCAGAGAAGCCCAGAAACAGGCTTTAGACTAC
ATTAAAGAAGCTTTTGGGGACAAATATATTCCCGATAAAGTGAAGGTATATAAGGCAAAAGAAGGTTCCCAGGATGCTCA
TGAGGCTATTCGTCCTACTTCAGTTGACCGTACTCCAGGTAAGGTAAAAAAATATTTAAACAAAGACCAGTACAGGTTAT
ATAAGTTGATCTGGGAAAGGTTTGTTGCCAGTCAAATGAGTCCAGCTCAGTATAAACAGGTGAAAGTATTGATAAAAGCA
GGAGATAAATATATTTTCAGGGCAAAGGGATCCAGAATTATATTCCCGGGGTTTTTACGGGTTAACACAAGCAGTCAGAA
AAAAGACATCATATTACCACCTGTTAAAAAGTCTGAAAGACTTGATGTTAAGGAAATCAAGCCAGAACAGCACTTTACCC
AGCCACCACCACGTTATACGGAAGCTACCCTGGTTAAAACATTAGAAGAAGAGGGAATAGGCCGCCCCAGTACTTATGCT
CCGATTATTTCAACAATAATCTCCCGGGGCTATGTGGAGCGTCAGGGTAAGCAGTTAAAACCGACTGAACTGGGTTTTAT
AGTTACAGATCTGTTATCAAAATATTTTCCTGATGTAACTGACATAGAATTTACCGCACATATGGAAGAAAGGCTTGATA
AGATAGAAGATGGCAAAGATGAATGGCGTAATGTGCTTGAAGATTTTTATTCCAATTTTTCCAGGAGACTTAAAGAGGCC
AGTGAGGAAATGGAAGAAGTTAAACTTGAAGATGAGGTAACTGATGAAGTATGTGAAAAGTGTGGCAGGAATATGGTAAT
AAAATATGGTCGTTATGGCAAATTTCTGGCCTGTTCTGGTTATCCTGAATGTAAAAATACCAAGCCTTATGTTATTAAAA
CTGGAGTTAAATGTCCTCAGTGTAAAGAGGGAGAGCTTGTTCAAAGAAAAAGCCGTAAAGGGCGTACTTTTTACGGATGT
AGTTCTTACCCTGATTGCAAATTTGTTGTCTGGAATAAACCAGTTAAAGAAAAATGCCCTGAATGTGGTGGCCTTATGGT
AGAGAAGAACTCAAAAAAGCAAGGCCGGTATTATCTCTGTATTAACAAAGAGTGTGGTTATAAGAAAGAAGTATAA

Upstream 100 bases:

>100_bases
CTGAAATACCTGAATTTCAGATATATTATAATGATCGACACAAGTATATAAATTAAAACGGTTATAACATAAAACCGGGT
GTCTAATAGGAGGTGCGCCC

Downstream 100 bases:

>100_bases
AGAGGTGGAATATATATGGATAGATTGATAGTAATTGGAGCAGGACTCGCAGGCTGTGAGGCAACCTGGCAGGCGATTAA
CAGAGGGGTTGAGGTCGATC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MGNKSNNTLVIVESPAKAKTISKFLGKGYKVEATMGHVIDLPKSKLGINIDKGFEPRYITIRGKGKVLKKLRKEVKKSKD
VLLATDPDREGEAISWHLTRALKIDEDKPRIEFNEITKSAIKNALKNRRPIDKNLVNSQQARRLLDRLVGYKLSPLLWKK
VRRGLSAGRVQTVAVKLLCNREKEIESFEPEEYWTISASFNKKDKDFIADLYRISGKKFKINNEKEAKQILEDLNKSKFV
VSDIKEKTRKRNPNPPFTTSTLQQRASSILGFSAKKTMYLAQQLYEGIDMGSEGTTGLISYIRTDSTRISREAQKQALDY
IKEAFGDKYIPDKVKVYKAKEGSQDAHEAIRPTSVDRTPGKVKKYLNKDQYRLYKLIWERFVASQMSPAQYKQVKVLIKA
GDKYIFRAKGSRIIFPGFLRVNTSSQKKDIILPPVKKSERLDVKEIKPEQHFTQPPPRYTEATLVKTLEEEGIGRPSTYA
PIISTIISRGYVERQGKQLKPTELGFIVTDLLSKYFPDVTDIEFTAHMEERLDKIEDGKDEWRNVLEDFYSNFSRRLKEA
SEEMEEVKLEDEVTDEVCEKCGRNMVIKYGRYGKFLACSGYPECKNTKPYVIKTGVKCPQCKEGELVQRKSRKGRTFYGC
SSYPDCKFVVWNKPVKEKCPECGGLMVEKNSKKQGRYYLCINKECGYKKEV

Sequences:

>Translated_691_residues
MGNKSNNTLVIVESPAKAKTISKFLGKGYKVEATMGHVIDLPKSKLGINIDKGFEPRYITIRGKGKVLKKLRKEVKKSKD
VLLATDPDREGEAISWHLTRALKIDEDKPRIEFNEITKSAIKNALKNRRPIDKNLVNSQQARRLLDRLVGYKLSPLLWKK
VRRGLSAGRVQTVAVKLLCNREKEIESFEPEEYWTISASFNKKDKDFIADLYRISGKKFKINNEKEAKQILEDLNKSKFV
VSDIKEKTRKRNPNPPFTTSTLQQRASSILGFSAKKTMYLAQQLYEGIDMGSEGTTGLISYIRTDSTRISREAQKQALDY
IKEAFGDKYIPDKVKVYKAKEGSQDAHEAIRPTSVDRTPGKVKKYLNKDQYRLYKLIWERFVASQMSPAQYKQVKVLIKA
GDKYIFRAKGSRIIFPGFLRVNTSSQKKDIILPPVKKSERLDVKEIKPEQHFTQPPPRYTEATLVKTLEEEGIGRPSTYA
PIISTIISRGYVERQGKQLKPTELGFIVTDLLSKYFPDVTDIEFTAHMEERLDKIEDGKDEWRNVLEDFYSNFSRRLKEA
SEEMEEVKLEDEVTDEVCEKCGRNMVIKYGRYGKFLACSGYPECKNTKPYVIKTGVKCPQCKEGELVQRKSRKGRTFYGC
SSYPDCKFVVWNKPVKEKCPECGGLMVEKNSKKQGRYYLCINKECGYKKEV
>Mature_690_residues
GNKSNNTLVIVESPAKAKTISKFLGKGYKVEATMGHVIDLPKSKLGINIDKGFEPRYITIRGKGKVLKKLRKEVKKSKDV
LLATDPDREGEAISWHLTRALKIDEDKPRIEFNEITKSAIKNALKNRRPIDKNLVNSQQARRLLDRLVGYKLSPLLWKKV
RRGLSAGRVQTVAVKLLCNREKEIESFEPEEYWTISASFNKKDKDFIADLYRISGKKFKINNEKEAKQILEDLNKSKFVV
SDIKEKTRKRNPNPPFTTSTLQQRASSILGFSAKKTMYLAQQLYEGIDMGSEGTTGLISYIRTDSTRISREAQKQALDYI
KEAFGDKYIPDKVKVYKAKEGSQDAHEAIRPTSVDRTPGKVKKYLNKDQYRLYKLIWERFVASQMSPAQYKQVKVLIKAG
DKYIFRAKGSRIIFPGFLRVNTSSQKKDIILPPVKKSERLDVKEIKPEQHFTQPPPRYTEATLVKTLEEEGIGRPSTYAP
IISTIISRGYVERQGKQLKPTELGFIVTDLLSKYFPDVTDIEFTAHMEERLDKIEDGKDEWRNVLEDFYSNFSRRLKEAS
EEMEEVKLEDEVTDEVCEKCGRNMVIKYGRYGKFLACSGYPECKNTKPYVIKTGVKCPQCKEGELVQRKSRKGRTFYGCS
SYPDCKFVVWNKPVKEKCPECGGLMVEKNSKKQGRYYLCINKECGYKKEV

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=745, Percent_Identity=24.9664429530201, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI10835218, Length=604, Percent_Identity=25.4966887417219, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1787529, Length=750, Percent_Identity=39.2, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI48994931, Length=116, Percent_Identity=45.6896551724138, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788061, Length=627, Percent_Identity=22.6475279106858, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=595, Percent_Identity=25.7142857142857, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17555378, Length=507, Percent_Identity=26.0355029585799, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24585251, Length=655, Percent_Identity=25.0381679389313, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640096, Length=655, Percent_Identity=24.5801526717557, Blast_Score=129, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79410; Mature: 79278

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKSNNTLVIVESPAKAKTISKFLGKGYKVEATMGHVIDLPKSKLGINIDKGFEPRYIT
CCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEECCHHHCCCCHHHCEEECCCCCCCEEE
IRGKGKVLKKLRKEVKKSKDVLLATDPDREGEAISWHLTRALKIDEDKPRIEFNEITKSA
EECCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHH
IKNALKNRRPIDKNLVNSQQARRLLDRLVGYKLSPLLWKKVRRGLSAGRVQTVAVKLLCN
HHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHC
REKEIESFEPEEYWTISASFNKKDKDFIADLYRISGKKFKINNEKEAKQILEDLNKSKFV
CHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCH
VSDIKEKTRKRNPNPPFTTSTLQQRASSILGFSAKKTMYLAQQLYEGIDMGSEGTTGLIS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
YIRTDSTRISREAQKQALDYIKEAFGDKYIPDKVKVYKAKEGSQDAHEAIRPTSVDRTPG
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCHH
KVKKYLNKDQYRLYKLIWERFVASQMSPAQYKQVKVLIKAGDKYIFRAKGSRIIFPGFLR
HHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEHEECCCEEEEEECCCEEEECCEEE
VNTSSQKKDIILPPVKKSERLDVKEIKPEQHFTQPPPRYTEATLVKTLEEEGIGRPSTYA
ECCCCCCCCEECCCCCCCCCCCHHHCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
PIISTIISRGYVERQGKQLKPTELGFIVTDLLSKYFPDVTDIEFTAHMEERLDKIEDGKD
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
EWRNVLEDFYSNFSRRLKEASEEMEEVKLEDEVTDEVCEKCGRNMVIKYGRYGKFLACSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECC
YPECKNTKPYVIKTGVKCPQCKEGELVQRKSRKGRTFYGCSSYPDCKFVVWNKPVKEKCP
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCEEEEECCCHHHHCC
ECGGLMVEKNSKKQGRYYLCINKECGYKKEV
CCCCEEEECCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
GNKSNNTLVIVESPAKAKTISKFLGKGYKVEATMGHVIDLPKSKLGINIDKGFEPRYIT
CCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEECCHHHCCCCHHHCEEECCCCCCCEEE
IRGKGKVLKKLRKEVKKSKDVLLATDPDREGEAISWHLTRALKIDEDKPRIEFNEITKSA
EECCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHH
IKNALKNRRPIDKNLVNSQQARRLLDRLVGYKLSPLLWKKVRRGLSAGRVQTVAVKLLCN
HHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHC
REKEIESFEPEEYWTISASFNKKDKDFIADLYRISGKKFKINNEKEAKQILEDLNKSKFV
CHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCH
VSDIKEKTRKRNPNPPFTTSTLQQRASSILGFSAKKTMYLAQQLYEGIDMGSEGTTGLIS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
YIRTDSTRISREAQKQALDYIKEAFGDKYIPDKVKVYKAKEGSQDAHEAIRPTSVDRTPG
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCHH
KVKKYLNKDQYRLYKLIWERFVASQMSPAQYKQVKVLIKAGDKYIFRAKGSRIIFPGFLR
HHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEHEECCCEEEEEECCCEEEECCEEE
VNTSSQKKDIILPPVKKSERLDVKEIKPEQHFTQPPPRYTEATLVKTLEEEGIGRPSTYA
ECCCCCCCCEECCCCCCCCCCCHHHCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
PIISTIISRGYVERQGKQLKPTELGFIVTDLLSKYFPDVTDIEFTAHMEERLDKIEDGKD
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
EWRNVLEDFYSNFSRRLKEASEEMEEVKLEDEVTDEVCEKCGRNMVIKYGRYGKFLACSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECC
YPECKNTKPYVIKTGVKCPQCKEGELVQRKSRKGRTFYGCSSYPDCKFVVWNKPVKEKCP
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCEEEEECCCHHHHCC
ECGGLMVEKNSKKQGRYYLCINKECGYKKEV
CCCCEEEECCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]