Click here to switch to the map view.

The map label for this gene is nudF [H]

Identifier: 220931527

GI number: 220931527

Start: 740372

End: 740902

Strand: Direct

Name: nudF [H]

Synonym: Hore_06830

Alternate gene names: 220931527

Gene position: 740372-740902 (Clockwise)

Preceding gene: 220931526

Following gene: 220931528

Centisome position: 28.72

GC content: 35.4

Gene sequence:

>531_bases
ATGGAACTTGAAGAAAAAACAATTTCAACTGAAGAGGTGTTTCAGGGACGTATTATAGACGTAAGAATAGATAAAGTTAA
ACTTCCCAATGGAAAAGAAGCCAGTCGTGAAGTAGTAGAACACCCCGGTGGGGTTACCATTATTCCTGTAATGGGTAATG
AAGTTATTTTAGTGGAGCAGTTTCGGAAGCCGGCTAATAAATGTTTACTAGAACTTCCAGCAGGTAAATTAGAGGAGGGA
GAGACCCCTGCTTTTTGTGCTGAAAGGGAGTTGATAGAAGAGATAGGCTTTAAACCAGGGAAATTGGATTTTTTATTTTC
ATTTTATACAAGCCCGGGTTTTAGTAATGAGATCCTCTATTTATTTCTGGCTAGAGAATTAAAGCCATTTGAGGATAAGG
GTGACGAAGATGAATTTATTAACATACATAAATTAAAAATAGATGATGTTTTAGAATACATAGAATCAGGTAAAATTGAA
GATGCCAAAACTATAATAGGGCTTTTATATTTACTGAGAGAGGTGTTGTAA

Upstream 100 bases:

>100_bases
TTAATTCCATGGGCAGGGGATATGAAGATGACCACCTGTTTTTCATTACAGCAGGATTAGCTGTTTTGAGGTTTAATGAT
ATGAAAAAGGGGTGATTGTA

Downstream 100 bases:

>100_bases
GCTTTGCAGGAAATATATGCAGATCTTCATATACACATTGGCAGTGCCTGTGGGGGGCAGCCCGTAAAGGTGACTGCTTC
CAGGAAACTTAACTTTCAAA

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MELEEKTISTEEVFQGRIIDVRIDKVKLPNGKEASREVVEHPGGVTIIPVMGNEVILVEQFRKPANKCLLELPAGKLEEG
ETPAFCAERELIEEIGFKPGKLDFLFSFYTSPGFSNEILYLFLARELKPFEDKGDEDEFINIHKLKIDDVLEYIESGKIE
DAKTIIGLLYLLREVL

Sequences:

>Translated_176_residues
MELEEKTISTEEVFQGRIIDVRIDKVKLPNGKEASREVVEHPGGVTIIPVMGNEVILVEQFRKPANKCLLELPAGKLEEG
ETPAFCAERELIEEIGFKPGKLDFLFSFYTSPGFSNEILYLFLARELKPFEDKGDEDEFINIHKLKIDDVLEYIESGKIE
DAKTIIGLLYLLREVL
>Mature_176_residues
MELEEKTISTEEVFQGRIIDVRIDKVKLPNGKEASREVVEHPGGVTIIPVMGNEVILVEQFRKPANKCLLELPAGKLEEG
ETPAFCAERELIEEIGFKPGKLDFLFSFYTSPGFSNEILYLFLARELKPFEDKGDEDEFINIHKLKIDDVLEYIESGKIE
DAKTIIGLLYLLREVL

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI37594464, Length=182, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1789412, Length=179, Percent_Identity=30.7262569832402, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788810, Length=147, Percent_Identity=34.6938775510204, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20030; Mature: 20030

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEEKTISTEEVFQGRIIDVRIDKVKLPNGKEASREVVEHPGGVTIIPVMGNEVILVEQ
CCCCHHCCCHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCEEEEHH
FRKPANKCLLELPAGKLEEGETPAFCAERELIEEIGFKPGKLDFLFSFYTSPGFSNEILY
HCCCHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCHHHH
LFLARELKPFEDKGDEDEFINIHKLKIDDVLEYIESGKIEDAKTIIGLLYLLREVL
EEHHHHCCCCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MELEEKTISTEEVFQGRIIDVRIDKVKLPNGKEASREVVEHPGGVTIIPVMGNEVILVEQ
CCCCHHCCCHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCEEEEHH
FRKPANKCLLELPAGKLEEGETPAFCAERELIEEIGFKPGKLDFLFSFYTSPGFSNEILY
HCCCHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCHHHH
LFLARELKPFEDKGDEDEFINIHKLKIDDVLEYIESGKIEDAKTIIGLLYLLREVL
EEHHHHCCCCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]