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The map label for this gene is tyrS [H]

Identifier: 220931414

GI number: 220931414

Start: 624034

End: 625257

Strand: Direct

Name: tyrS [H]

Synonym: Hore_05700

Alternate gene names: 220931414

Gene position: 624034-625257 (Clockwise)

Preceding gene: 220931412

Following gene: 220931415

Centisome position: 24.2

GC content: 38.4

Gene sequence:

>1224_bases
TTGGATATAAATGAACAGCTAAATATTTTAAAAAGAGGGGTTAGTGAATTAATAACAGAAAAGGAGTTGAAAAAGAAATT
AGAGCTAGCCAGAGAAGAAGGACGTCCCCTGCGGGTAAAACTGGGACTGGACCCCACAGCACCTGATATTCATCTTGGTC
ATAGTGTTGTTTTAAGGAAGTTAAAACAATTTCAGGACTTAGGCCATGAGGTATACCTTATAATTGGCGATTTTACCGGA
ATGATTGGAGACCCTACCGGGAAGTCGGTTACCAGAAAACAATTAACAGAAGAAGAAGTCAGGGAAAATGCTAAAACCTA
TCGGGAACAGTTTTCCAAAATACTTAACCCCGATAAGACAAAGCTTGTTTTCAATTCTAATTGGCTTTCAGACCTGAATT
TATCTGATGTTATTGAGTTATCTTCTAAATATACTGTTGCCAGAATGCTGGAAAGGGAAGATTTCTCCCAGAGGTATAAA
AGTGGTAAACCGATTAGTATACATGAGTTTATTTACCCTTTAATGCAGGGTTATGATTCGGTTGCCATAAAAGCTGACAT
TGAACTTGGAGGAACTGACCAGAAGTTTAATTTACTGGTGGGGAGACACCTTCAAAAAGAATTCGGGCAGAAGCCCCAGG
TAATCATGATGATGCCCATCCTGGAAGGCCTGGATGGGGTTAACAAGATGAGTAAAAGCCTTGATAATTATATTGGAATT
AACGATGAACCTAAAGACATGTATGGTAAGGTTATGTCCATACCGGATAGTTTAATAACCAGGTATTTTGAATTATTGAC
CGATGTGCCTGAAGATAAAATAAATGAAATGAAGAAGAGCATGGAAGAAGACCGGGTTAATCCAATGAATTTCAAAAAAG
AGCTGGCCAGGATTATAGTTACTGAATACCATGGTCAGGATGCTGCCAGGGAAGCTGAAAAGGAGTTTAAGCGGGTTTTT
TCCCAGGGTAAACTCCCTGAAGATATTCCGGAAGTAATAATTGAGCAGAAAGATCTGGAAGATGGGAAGATGTGGATTGT
TCATTTGATAGCGGCTACAGGGCTTGTTAAGAGTAATAGTGAAGCTCGCAGGATGATTAAACAGGGAGCAGTTTCCATAA
ATGATGAAAAACATAAAAGTATCAATGAGGACATTAAAGTAGAAGATGGAATGATTATTAGAATTGGCAAGAGAAGGTTT
GCTAAAATTAAACTTCCGGATTAA

Upstream 100 bases:

>100_bases
TACATTTTATCAATTTATATATTTGTTATATATTTGCATGTTATATTTTGTATAGAAAAATTTAAATAAAAAGTGTTTAT
TATAATTGGAGGTGTAAATA

Downstream 100 bases:

>100_bases
ACTAAAAATAAACTAAGAACTTATTTCCCCCCTCCTGCATAATATGTTAGGAGGGGGGATATCTTTGTTTTTAAAAAGGG
GTATATTACTTAAGTTTTTT

Product: Tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MDINEQLNILKRGVSELITEKELKKKLELAREEGRPLRVKLGLDPTAPDIHLGHSVVLRKLKQFQDLGHEVYLIIGDFTG
MIGDPTGKSVTRKQLTEEEVRENAKTYREQFSKILNPDKTKLVFNSNWLSDLNLSDVIELSSKYTVARMLEREDFSQRYK
SGKPISIHEFIYPLMQGYDSVAIKADIELGGTDQKFNLLVGRHLQKEFGQKPQVIMMMPILEGLDGVNKMSKSLDNYIGI
NDEPKDMYGKVMSIPDSLITRYFELLTDVPEDKINEMKKSMEEDRVNPMNFKKELARIIVTEYHGQDAAREAEKEFKRVF
SQGKLPEDIPEVIIEQKDLEDGKMWIVHLIAATGLVKSNSEARRMIKQGAVSINDEKHKSINEDIKVEDGMIIRIGKRRF
AKIKLPD

Sequences:

>Translated_407_residues
MDINEQLNILKRGVSELITEKELKKKLELAREEGRPLRVKLGLDPTAPDIHLGHSVVLRKLKQFQDLGHEVYLIIGDFTG
MIGDPTGKSVTRKQLTEEEVRENAKTYREQFSKILNPDKTKLVFNSNWLSDLNLSDVIELSSKYTVARMLEREDFSQRYK
SGKPISIHEFIYPLMQGYDSVAIKADIELGGTDQKFNLLVGRHLQKEFGQKPQVIMMMPILEGLDGVNKMSKSLDNYIGI
NDEPKDMYGKVMSIPDSLITRYFELLTDVPEDKINEMKKSMEEDRVNPMNFKKELARIIVTEYHGQDAAREAEKEFKRVF
SQGKLPEDIPEVIIEQKDLEDGKMWIVHLIAATGLVKSNSEARRMIKQGAVSINDEKHKSINEDIKVEDGMIIRIGKRRF
AKIKLPD
>Mature_407_residues
MDINEQLNILKRGVSELITEKELKKKLELAREEGRPLRVKLGLDPTAPDIHLGHSVVLRKLKQFQDLGHEVYLIIGDFTG
MIGDPTGKSVTRKQLTEEEVRENAKTYREQFSKILNPDKTKLVFNSNWLSDLNLSDVIELSSKYTVARMLEREDFSQRYK
SGKPISIHEFIYPLMQGYDSVAIKADIELGGTDQKFNLLVGRHLQKEFGQKPQVIMMMPILEGLDGVNKMSKSLDNYIGI
NDEPKDMYGKVMSIPDSLITRYFELLTDVPEDKINEMKKSMEEDRVNPMNFKKELARIIVTEYHGQDAAREAEKEFKRVF
SQGKLPEDIPEVIIEQKDLEDGKMWIVHLIAATGLVKSNSEARRMIKQGAVSINDEKHKSINEDIKVEDGMIIRIGKRRF
AKIKLPD

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=406, Percent_Identity=25.615763546798, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=27.0783847980998, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=400, Percent_Identity=26, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325160, Length=416, Percent_Identity=25.4807692307692, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321624, Length=338, Percent_Identity=24.5562130177515, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130383, Length=406, Percent_Identity=25.8620689655172, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46703; Mature: 46703

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINEQLNILKRGVSELITEKELKKKLELAREEGRPLRVKLGLDPTAPDIHLGHSVVLRK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHH
LKQFQDLGHEVYLIIGDFTGMIGDPTGKSVTRKQLTEEEVRENAKTYREQFSKILNPDKT
HHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLVFNSNWLSDLNLSDVIELSSKYTVARMLEREDFSQRYKSGKPISIHEFIYPLMQGYDS
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCC
VAIKADIELGGTDQKFNLLVGRHLQKEFGQKPQVIMMMPILEGLDGVNKMSKSLDNYIGI
EEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCHHHHHHHHHHHHCCC
NDEPKDMYGKVMSIPDSLITRYFELLTDVPEDKINEMKKSMEEDRVNPMNFKKELARIIV
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TEYHGQDAAREAEKEFKRVFSQGKLPEDIPEVIIEQKDLEDGKMWIVHLIAATGLVKSNS
HHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
EARRMIKQGAVSINDEKHKSINEDIKVEDGMIIRIGKRRFAKIKLPD
HHHHHHHHCCCCCCCHHHHCCCCCCEECCCEEEEECCCCEEEEECCC
>Mature Secondary Structure
MDINEQLNILKRGVSELITEKELKKKLELAREEGRPLRVKLGLDPTAPDIHLGHSVVLRK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHH
LKQFQDLGHEVYLIIGDFTGMIGDPTGKSVTRKQLTEEEVRENAKTYREQFSKILNPDKT
HHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLVFNSNWLSDLNLSDVIELSSKYTVARMLEREDFSQRYKSGKPISIHEFIYPLMQGYDS
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCC
VAIKADIELGGTDQKFNLLVGRHLQKEFGQKPQVIMMMPILEGLDGVNKMSKSLDNYIGI
EEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCHHHHHHHHHHHHCCC
NDEPKDMYGKVMSIPDSLITRYFELLTDVPEDKINEMKKSMEEDRVNPMNFKKELARIIV
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TEYHGQDAAREAEKEFKRVFSQGKLPEDIPEVIIEQKDLEDGKMWIVHLIAATGLVKSNS
HHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
EARRMIKQGAVSINDEKHKSINEDIKVEDGMIIRIGKRRFAKIKLPD
HHHHHHHHCCCCCCCHHHHCCCCCCEECCCEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA