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The map label for this gene is pheS [H]

Identifier: 220931407

GI number: 220931407

Start: 612320

End: 613345

Strand: Direct

Name: pheS [H]

Synonym: Hore_05630

Alternate gene names: 220931407

Gene position: 612320-613345 (Clockwise)

Preceding gene: 220931406

Following gene: 220931408

Centisome position: 23.75

GC content: 40.55

Gene sequence:

>1026_bases
ATGGATTTAAAAAAACAGTTAGAAGAAATAAAGACAAAGGCAGAAAAAGAGTTTCAGGATGTAAAAGACCTGGATCACCT
GGAACAACTAAGGGTAAAGTATCTTGGTAAAAAGGGAGAAATAACTTCAGTATTAAGGCAGATGGGAAAAATTCCTGCCG
AAGAGAGGCCGGTTATAGGAAAGAAGGCCAATATACTTAAAAATAAATTAAATAATATAATTATTAAGAAAAAGGCTGAA
TTAGAAGAACTGGCCAAACAGAGAAAGCTTGAAAAGGAAAAAATTGATGTGACCCTGCCCGGGGTCAGGCCCGAAGTGGG
TCATAGACATCCTTTAAATCAGGTTATAGATGAAATAAAAGATATTTTTATTGGACTTGGATTTACTATTGCTGAAGGAC
CAGAAGTTGAGAGTGAGTATAACAACTTTGAGGCTTTAAATATTCCAGCCCACCATCCGGCCCGGGATTTACAGGATACC
CTGTATTTATACGATGATTTGCTTTTAAGAACCCATACTTCCCCAGTTCAGATAAGGACAATGGCTAAGCAAAACCCACC
CATCAGGGTAATTGCACCTGGTCGTGTTTACCGGGCTGATGAGCTGGATGCTTCTCATTCCCCAATCTTCCATCAGGTAG
AGGGTCTGGTTATTAATGAAAATATCTCCTTTAGTGATCTTAAAGGGACCATTGAGACTGTTGTTCATGCCCTTTTTGGA
AAGGATAGTAAAATCAGGTTTAGACCAAGTTATTTCCCCTTTACAGAACCCAGTGCCGAGGTGGATGTGTCCTGTAATGT
ATGTCATGGTGAAGGGTGTAGCCTCTGTTCCCGGACAGGTTGGCTGGAAATTATGGGGTCTGGTATGGTCCACCCCAATG
TCCTGGAAATGGCTGGAATTGACCCGGTTAAGTATAGTGGATTTGCCTTTGGAATGGGCCTGGATCGGATTGCTATCCTG
AAATATAAGCTTGATGACATCAGGTTGTTATATGAAAATGACCGTAGGTTTTTACATCAGTTTTAA

Upstream 100 bases:

>100_bases
TTTCTATATTTTTGTTCTGGTGATACTGGATTCCTGATTTTTTATATTGGTAAAAAAATATTGATAATTATTTTTTAAAT
AAAGTGGAGGTGTTACGTGC

Downstream 100 bases:

>100_bases
CTTGAGGTTTATTTAGACCTGAAAAGTTTTAAATACCTGACCAGTTTTAAATATATGTTTTATTAAGCCCTGATTCAAGA
TTTTAATAAAAATAATTAGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MDLKKQLEEIKTKAEKEFQDVKDLDHLEQLRVKYLGKKGEITSVLRQMGKIPAEERPVIGKKANILKNKLNNIIIKKKAE
LEELAKQRKLEKEKIDVTLPGVRPEVGHRHPLNQVIDEIKDIFIGLGFTIAEGPEVESEYNNFEALNIPAHHPARDLQDT
LYLYDDLLLRTHTSPVQIRTMAKQNPPIRVIAPGRVYRADELDASHSPIFHQVEGLVINENISFSDLKGTIETVVHALFG
KDSKIRFRPSYFPFTEPSAEVDVSCNVCHGEGCSLCSRTGWLEIMGSGMVHPNVLEMAGIDPVKYSGFAFGMGLDRIAIL
KYKLDDIRLLYENDRRFLHQF

Sequences:

>Translated_341_residues
MDLKKQLEEIKTKAEKEFQDVKDLDHLEQLRVKYLGKKGEITSVLRQMGKIPAEERPVIGKKANILKNKLNNIIIKKKAE
LEELAKQRKLEKEKIDVTLPGVRPEVGHRHPLNQVIDEIKDIFIGLGFTIAEGPEVESEYNNFEALNIPAHHPARDLQDT
LYLYDDLLLRTHTSPVQIRTMAKQNPPIRVIAPGRVYRADELDASHSPIFHQVEGLVINENISFSDLKGTIETVVHALFG
KDSKIRFRPSYFPFTEPSAEVDVSCNVCHGEGCSLCSRTGWLEIMGSGMVHPNVLEMAGIDPVKYSGFAFGMGLDRIAIL
KYKLDDIRLLYENDRRFLHQF
>Mature_341_residues
MDLKKQLEEIKTKAEKEFQDVKDLDHLEQLRVKYLGKKGEITSVLRQMGKIPAEERPVIGKKANILKNKLNNIIIKKKAE
LEELAKQRKLEKEKIDVTLPGVRPEVGHRHPLNQVIDEIKDIFIGLGFTIAEGPEVESEYNNFEALNIPAHHPARDLQDT
LYLYDDLLLRTHTSPVQIRTMAKQNPPIRVIAPGRVYRADELDASHSPIFHQVEGLVINENISFSDLKGTIETVVHALFG
KDSKIRFRPSYFPFTEPSAEVDVSCNVCHGEGCSLCSRTGWLEIMGSGMVHPNVLEMAGIDPVKYSGFAFGMGLDRIAIL
KYKLDDIRLLYENDRRFLHQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=277, Percent_Identity=31.7689530685921, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI5729820, Length=242, Percent_Identity=34.7107438016529, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1788007, Length=337, Percent_Identity=50.7418397626113, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17508957, Length=288, Percent_Identity=31.5972222222222, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=300, Percent_Identity=33.6666666666667, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32563657, Length=288, Percent_Identity=31.5972222222222, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321087, Length=274, Percent_Identity=33.2116788321168, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325304, Length=285, Percent_Identity=30.1754385964912, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18858079, Length=274, Percent_Identity=30.2919708029197, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17137424, Length=290, Percent_Identity=27.5862068965517, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38804; Mature: 38804

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKKQLEEIKTKAEKEFQDVKDLDHLEQLRVKYLGKKGEITSVLRQMGKIPAEERPVIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
KKANILKNKLNNIIIKKKAELEELAKQRKLEKEKIDVTLPGVRPEVGHRHPLNQVIDEIK
CHHHHHHHHHHHEEEECHHHHHHHHHHHCCHHHHCCEECCCCCCCCCCCCCHHHHHHHHH
DIFIGLGFTIAEGPEVESEYNNFEALNIPAHHPARDLQDTLYLYDDLLLRTHTSPVQIRT
HHHHHCCEEECCCCCCCHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
MAKQNPPIRVIAPGRVYRADELDASHSPIFHQVEGLVINENISFSDLKGTIETVVHALFG
HHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHC
KDSKIRFRPSYFPFTEPSAEVDVSCNVCHGEGCSLCSRTGWLEIMGSGMVHPNVLEMAGI
CCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCHHHHCCCCCCCCHHHHCCC
DPVKYSGFAFGMGLDRIAILKYKLDDIRLLYENDRRFLHQF
CCCEECCEEECCCCCEEEEEEEECCCEEEEEHHHHHHHHCC
>Mature Secondary Structure
MDLKKQLEEIKTKAEKEFQDVKDLDHLEQLRVKYLGKKGEITSVLRQMGKIPAEERPVIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
KKANILKNKLNNIIIKKKAELEELAKQRKLEKEKIDVTLPGVRPEVGHRHPLNQVIDEIK
CHHHHHHHHHHHEEEECHHHHHHHHHHHCCHHHHCCEECCCCCCCCCCCCCHHHHHHHHH
DIFIGLGFTIAEGPEVESEYNNFEALNIPAHHPARDLQDTLYLYDDLLLRTHTSPVQIRT
HHHHHCCEEECCCCCCCHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
MAKQNPPIRVIAPGRVYRADELDASHSPIFHQVEGLVINENISFSDLKGTIETVVHALFG
HHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHC
KDSKIRFRPSYFPFTEPSAEVDVSCNVCHGEGCSLCSRTGWLEIMGSGMVHPNVLEMAGI
CCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCHHHHCCCCCCCCHHHHCCC
DPVKYSGFAFGMGLDRIAILKYKLDDIRLLYENDRRFLHQF
CCCEECCEEECCCCCEEEEEEEECCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]