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The map label for this gene is ysgA [H]

Identifier: 220931406

GI number: 220931406

Start: 610857

End: 611669

Strand: Direct

Name: ysgA [H]

Synonym: Hore_05620

Alternate gene names: 220931406

Gene position: 610857-611669 (Clockwise)

Preceding gene: 220931405

Following gene: 220931407

Centisome position: 23.69

GC content: 36.29

Gene sequence:

>813_bases
ATGAAAGAAGTAATTAAAAGTAGTGATAATAAAAACTTTAAATATTTAAAAAAGCTTTATAATAAGTCCTTCAGGAAGAA
AGAAGGCAAGTTTATTCTTGAGGGTTATAGGTTAATCATGGAGGCCTGCGGAAAGGCGCGGTTTGATAGAATATTTATGA
CACCTTATTTTGCTAAGACAGATGAAGGTGAAGAAATAATAAGAAAATTTAAAGACCACATTCCTGTCCATTTTGTTACC
GGAAAACTGCTTTATAATGTGGCTGATACTGAAAATCCCCAGGGAATAGCAGCCATTACTTACGTGCCCCGGTCCGGGGC
TAATATCCTATTTCATAACAGGAATAGAGGTATTATTCTAATCCTTGATAGGATTCAGGACCCGGGGAATATGGGAACTA
TTATTAGAACTGCCGTTGCTGCCGGTATTGAAGGAATAATTTGCCTGAAGGGTAGTGTTGATATTTATAACCTCAAAGTT
ATCAGGGCCAGTATGGGTGCTATTTTTAGTATACCAATCTTAACAGGGGTTTCCTTTAAAAGGTTGCAGAAAATCAGGGA
CGAATACCCTGATTATAAATTGATTTGCACTGATTTAAATACAAGTAAATTTTACCATGAGATAGACTATCAAGAACCCC
TTATGCTTGTTATCGGGAATGAAGCCAGAGGTATAAGAGAGGAATTATTATCCCTGGCCGACATAAAGATTAAGATACCA
TTAATGGGAAATATTGACTCGTTAAATGCGGCAGTATCAACAGCTATTGTACTATATGATATAGTTGTTAAATGCAGGCA
TAGCAACGGTTAA

Upstream 100 bases:

>100_bases
TTTTTACTTTTAAAGTTTTAACTTTTAAGTGTTATGATTTTAATTTAGTTTAAATCATTTTAATTTTCCTTTTAATTATT
TGACTGGCGGTGTCCAGATT

Downstream 100 bases:

>100_bases
TAACATAAGTAATGAAGATTCAGAAATTTTTTTATTAATTTATTTTATTCCTTGTCAAGCAGTTTCTGGTATGGTATAAT
CAAAAGTGAAGTGAACAATA

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKEVIKSSDNKNFKYLKKLYNKSFRKKEGKFILEGYRLIMEACGKARFDRIFMTPYFAKTDEGEEIIRKFKDHIPVHFVT
GKLLYNVADTENPQGIAAITYVPRSGANILFHNRNRGIILILDRIQDPGNMGTIIRTAVAAGIEGIICLKGSVDIYNLKV
IRASMGAIFSIPILTGVSFKRLQKIRDEYPDYKLICTDLNTSKFYHEIDYQEPLMLVIGNEARGIREELLSLADIKIKIP
LMGNIDSLNAAVSTAIVLYDIVVKCRHSNG

Sequences:

>Translated_270_residues
MKEVIKSSDNKNFKYLKKLYNKSFRKKEGKFILEGYRLIMEACGKARFDRIFMTPYFAKTDEGEEIIRKFKDHIPVHFVT
GKLLYNVADTENPQGIAAITYVPRSGANILFHNRNRGIILILDRIQDPGNMGTIIRTAVAAGIEGIICLKGSVDIYNLKV
IRASMGAIFSIPILTGVSFKRLQKIRDEYPDYKLICTDLNTSKFYHEIDYQEPLMLVIGNEARGIREELLSLADIKIKIP
LMGNIDSLNAAVSTAIVLYDIVVKCRHSNG
>Mature_270_residues
MKEVIKSSDNKNFKYLKKLYNKSFRKKEGKFILEGYRLIMEACGKARFDRIFMTPYFAKTDEGEEIIRKFKDHIPVHFVT
GKLLYNVADTENPQGIAAITYVPRSGANILFHNRNRGIILILDRIQDPGNMGTIIRTAVAAGIEGIICLKGSVDIYNLKV
IRASMGAIFSIPILTGVSFKRLQKIRDEYPDYKLICTDLNTSKFYHEIDYQEPLMLVIGNEARGIREELLSLADIKIKIP
LMGNIDSLNAAVSTAIVLYDIVVKCRHSNG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=303, Percent_Identity=27.0627062706271, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=28.7581699346405, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790083, Length=157, Percent_Identity=27.3885350318471, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666840, Length=287, Percent_Identity=27.5261324041812, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30583; Mature: 30583

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVIKSSDNKNFKYLKKLYNKSFRKKEGKFILEGYRLIMEACGKARFDRIFMTPYFAKT
CCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCEEEECCCCCCC
DEGEEIIRKFKDHIPVHFVTGKLLYNVADTENPQGIAAITYVPRSGANILFHNRNRGIIL
CCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCEEE
ILDRIQDPGNMGTIIRTAVAAGIEGIICLKGSVDIYNLKVIRASMGAIFSIPILTGVSFK
EEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEHHCCEEEEHHHCCCCHH
RLQKIRDEYPDYKLICTDLNTSKFYHEIDYQEPLMLVIGNEARGIREELLSLADIKIKIP
HHHHHHHHCCCEEEEEEECCCCHHEEECCCCCCEEEEECCCCCHHHHHHHHHHCEEEEEE
LMGNIDSLNAAVSTAIVLYDIVVKCRHSNG
EECCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKEVIKSSDNKNFKYLKKLYNKSFRKKEGKFILEGYRLIMEACGKARFDRIFMTPYFAKT
CCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCEEEECCCCCCC
DEGEEIIRKFKDHIPVHFVTGKLLYNVADTENPQGIAAITYVPRSGANILFHNRNRGIIL
CCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCEEE
ILDRIQDPGNMGTIIRTAVAAGIEGIICLKGSVDIYNLKVIRASMGAIFSIPILTGVSFK
EEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEHHCCEEEEHHHCCCCHH
RLQKIRDEYPDYKLICTDLNTSKFYHEIDYQEPLMLVIGNEARGIREELLSLADIKIKIP
HHHHHHHHCCCEEEEEEECCCCHHEEECCCCCCEEEEECCCCCHHHHHHHHHHCEEEEEE
LMGNIDSLNAAVSTAIVLYDIVVKCRHSNG
EECCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]