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The map label for this gene is cspB [H]

Identifier: 220931393

GI number: 220931393

Start: 598545

End: 598751

Strand: Reverse

Name: cspB [H]

Synonym: Hore_05490

Alternate gene names: 220931393

Gene position: 598751-598545 (Counterclockwise)

Preceding gene: 220931396

Following gene: 220931392

Centisome position: 23.22

GC content: 35.75

Gene sequence:

>207_bases
TTGATAATCTACAACGGTAAAGTGAAATGGTTTAGTGACAAAAAAGGTTTTGGTTTTATTGAAAGAGAAGCTGGAGATGA
TGTTTTTGTACATTTTTCTGCAATTCAAAAAGAAGGCTTCAAGAGTTTAGATGAAGGTCAAGAGGTAGAATTCGAAGTAG
TTCAAGGTGATCGCGGCCCACAGGCTGCAAATGTTGTTACATTATAA

Upstream 100 bases:

>100_bases
AATTGTTTGACTTTGCAATATTAGTGTGATATAGTTAATGGCATTGGGCATTTAAAAAAATTATTAGAACTTGCCCTTAG
AAAATTTAGGAGGAATTTAA

Downstream 100 bases:

>100_bases
AGTACATAATGCTTTAATGATATAGCAACTAAGCCCTGACTATATTAATGTAGTCAGGGTTTTTATTTGTATATATGTCA
TGGAAACATAAAAAACTATA

Product: putative cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 68; Mature: 68

Protein sequence:

>68_residues
MIIYNGKVKWFSDKKGFGFIEREAGDDVFVHFSAIQKEGFKSLDEGQEVEFEVVQGDRGPQAANVVTL

Sequences:

>Translated_68_residues
MIIYNGKVKWFSDKKGFGFIEREAGDDVFVHFSAIQKEGFKSLDEGQEVEFEVVQGDRGPQAANVVTL
>Mature_68_residues
MIIYNGKVKWFSDKKGFGFIEREAGDDVFVHFSAIQKEGFKSLDEGQEVEFEVVQGDRGPQAANVVTL

Specific function: Affects cell viability at low temperatures [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=65.625, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=62.5, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=60, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI1788126, Length=69, Percent_Identity=57.9710144927536, Blast_Score=79, Evalue=6e-17,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=59.375, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=56.4516129032258, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=48.4375, Blast_Score=68, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7640; Mature: 7640

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIYNGKVKWFSDKKGFGFIEREAGDDVFVHFSAIQKEGFKSLDEGQEVEFEVVQGDRGP
CEEECCEEEEEECCCCCEEEECCCCCEEEEEEEHHCCCCCCCCCCCCCEEEEEEECCCCC
QAANVVTL
CCCEEEEC
>Mature Secondary Structure
MIIYNGKVKWFSDKKGFGFIEREAGDDVFVHFSAIQKEGFKSLDEGQEVEFEVVQGDRGP
CEEECCEEEEEECCCCCEEEECCCCCEEEEEEEHHCCCCCCCCCCCCCEEEEEEECCCCC
QAANVVTL
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8294017; 10736231 [H]