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The map label for this gene is lplA [H]

Identifier: 220931353

GI number: 220931353

Start: 554906

End: 555904

Strand: Direct

Name: lplA [H]

Synonym: Hore_05080

Alternate gene names: 220931353

Gene position: 554906-555904 (Clockwise)

Preceding gene: 220931352

Following gene: 220931355

Centisome position: 21.52

GC content: 37.94

Gene sequence:

>999_bases
ATGGAGTTTAAACCAGAGATAATCTATTCACAATCATTTAATCCCTGGTATAATCTGGCCCTTGAGGAATATCTCCTAAA
TGGGGTTAGTGACAATGAGGTCATTCTTTACCTCTGGCAGAATGATAATACGGTTGTAGTTGGCCGTAATCAGAATGCCT
GGAAGGAATGTCGTTGTAAACTATTAGAAGAAGAGGGGGGTAAACTGGCCCGCCGGCTGTCCGGTGGTGGGGCTGTTTAT
CATGACCTGGGGAATTTAAATTTTACATTTTTAATGAAAGACAGCTTATATGACCTTAAACACCAGTTAAATGTTATCTT
AAATGCAGTCAAGATGGCCGGTATTGAGGCAGAATTTTCGGGCCGGAATGACCTCGTTGTTCAGGGGAAGAAATTTTCAG
GTAATGCTTTTTATAAAGCTAAAGGCTCTTCATATCACCATGGTACAATTCTGGTAAATTCTGATTTTAGTAAACTGATG
AGGTATCTTCAGGTTTCCAGTGACAAGATAAGATCCAAAGGAGTTGATTCTGTAAGGGCCCGGGTAATTAATCTAAAGGA
AGTAAACCCCGATATAAATATTGAAAAAATGAAAAAATTAATGGAAGAAAGTTTTATAAAGGCCTATGGTGACAATATAG
AAACCAGTCCGGAGGAGATAAACCCTGAAAAGCTACCAGAACTGGATAAGTTATACCAGAAGTATTCTTCCTGGGAATGG
AGGTATGGAAGAACCCCGGAATTTGATATAAAATTGGTCAACAGGTTTGACTGGGGAGGTATTGAACTGAACCTTGCCCT
TAAAAATGGTGTCATAACGGAGTCAACTATTTATTCAGATGCTATGGAGTCTGACCTTATTCGCAAAATTGCTTCATCAC
TAAAGGGAGTTCCCTTCAGGTTTGGCGATATAAATAATAGGTTAACAGGACTAAAGGAAGAAGAAGGAAAAAATATTATT
GATGATTTATTAACATGGCTTAAAAATAAGATTAGTTAA

Upstream 100 bases:

>100_bases
AATTTTAATAACATTTTTCCTTATTTACAGGTATTTACCTCAATCGGGAGGTAATAATATTAAAGAGGATAGTTTTAAAG
GATAAGGAAGGGAGTCAGGT

Downstream 100 bases:

>100_bases
GCAGGCAGGACTAATATTGGTTAAACAGGCAGGATTGTTAATACCCTGCCTGTTTTTTTACAGCAGGTTGTTTTAAAAGT
TTAAAAAGTTCCGGGTATAT

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MEFKPEIIYSQSFNPWYNLALEEYLLNGVSDNEVILYLWQNDNTVVVGRNQNAWKECRCKLLEEEGGKLARRLSGGGAVY
HDLGNLNFTFLMKDSLYDLKHQLNVILNAVKMAGIEAEFSGRNDLVVQGKKFSGNAFYKAKGSSYHHGTILVNSDFSKLM
RYLQVSSDKIRSKGVDSVRARVINLKEVNPDINIEKMKKLMEESFIKAYGDNIETSPEEINPEKLPELDKLYQKYSSWEW
RYGRTPEFDIKLVNRFDWGGIELNLALKNGVITESTIYSDAMESDLIRKIASSLKGVPFRFGDINNRLTGLKEEEGKNII
DDLLTWLKNKIS

Sequences:

>Translated_332_residues
MEFKPEIIYSQSFNPWYNLALEEYLLNGVSDNEVILYLWQNDNTVVVGRNQNAWKECRCKLLEEEGGKLARRLSGGGAVY
HDLGNLNFTFLMKDSLYDLKHQLNVILNAVKMAGIEAEFSGRNDLVVQGKKFSGNAFYKAKGSSYHHGTILVNSDFSKLM
RYLQVSSDKIRSKGVDSVRARVINLKEVNPDINIEKMKKLMEESFIKAYGDNIETSPEEINPEKLPELDKLYQKYSSWEW
RYGRTPEFDIKLVNRFDWGGIELNLALKNGVITESTIYSDAMESDLIRKIASSLKGVPFRFGDINNRLTGLKEEEGKNII
DDLLTWLKNKIS
>Mature_332_residues
MEFKPEIIYSQSFNPWYNLALEEYLLNGVSDNEVILYLWQNDNTVVVGRNQNAWKECRCKLLEEEGGKLARRLSGGGAVY
HDLGNLNFTFLMKDSLYDLKHQLNVILNAVKMAGIEAEFSGRNDLVVQGKKFSGNAFYKAKGSSYHHGTILVNSDFSKLM
RYLQVSSDKIRSKGVDSVRARVINLKEVNPDINIEKMKKLMEESFIKAYGDNIETSPEEINPEKLPELDKLYQKYSSWEW
RYGRTPEFDIKLVNRFDWGGIELNLALKNGVITESTIYSDAMESDLIRKIASSLKGVPFRFGDINNRLTGLKEEEGKNII
DDLLTWLKNKIS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=252, Percent_Identity=36.5079365079365, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI21729884, Length=252, Percent_Identity=36.5079365079365, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI21729882, Length=252, Percent_Identity=36.5079365079365, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI21729880, Length=252, Percent_Identity=36.5079365079365, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI21729878, Length=252, Percent_Identity=36.5079365079365, Blast_Score=158, Evalue=8e-39,
Organism=Escherichia coli, GI1790846, Length=301, Percent_Identity=41.5282392026578, Blast_Score=264, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI71981909, Length=257, Percent_Identity=34.2412451361868, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322415, Length=264, Percent_Identity=31.8181818181818, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI28573464, Length=336, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI221330307, Length=206, Percent_Identity=25.2427184466019, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38062; Mature: 38062

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKPEIIYSQSFNPWYNLALEEYLLNGVSDNEVILYLWQNDNTVVVGRNQNAWKECRCK
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHH
LLEEEGGKLARRLSGGGAVYHDLGNLNFTFLMKDSLYDLKHQLNVILNAVKMAGIEAEFS
HHHHCCHHHHHHHCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEECC
GRNDLVVQGKKFSGNAFYKAKGSSYHHGTILVNSDFSKLMRYLQVSSDKIRSKGVDSVRA
CCCCEEEECCCCCCCEEEEECCCCEECCEEEEECCHHHHHHHHHHCHHHHHHCCHHHHHH
RVINLKEVNPDINIEKMKKLMEESFIKAYGDNIETSPEEINPEKLPELDKLYQKYSSWEW
HEEEHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCC
RYGRTPEFDIKLVNRFDWGGIELNLALKNGVITESTIYSDAMESDLIRKIASSLKGVPFR
CCCCCCCCEEEEEEECCCCCEEEEEEEECCEEECHHHHHHHHHHHHHHHHHHHHCCCCEE
FGDINNRLTGLKEEEGKNIIDDLLTWLKNKIS
ECCCCCHHCCCCHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFKPEIIYSQSFNPWYNLALEEYLLNGVSDNEVILYLWQNDNTVVVGRNQNAWKECRCK
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHH
LLEEEGGKLARRLSGGGAVYHDLGNLNFTFLMKDSLYDLKHQLNVILNAVKMAGIEAEFS
HHHHCCHHHHHHHCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEECC
GRNDLVVQGKKFSGNAFYKAKGSSYHHGTILVNSDFSKLMRYLQVSSDKIRSKGVDSVRA
CCCCEEEECCCCCCCEEEEECCCCEECCEEEEECCHHHHHHHHHHCHHHHHHCCHHHHHH
RVINLKEVNPDINIEKMKKLMEESFIKAYGDNIETSPEEINPEKLPELDKLYQKYSSWEW
HEEEHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCC
RYGRTPEFDIKLVNRFDWGGIELNLALKNGVITESTIYSDAMESDLIRKIASSLKGVPFR
CCCCCCCCEEEEEEECCCCCEEEEEEEECCEEECHHHHHHHHHHHHHHHHHHHHCCCCEE
FGDINNRLTGLKEEEGKNIIDDLLTWLKNKIS
ECCCCCHHCCCCHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA