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The map label for this gene is nucI [H]

Identifier: 220931295

GI number: 220931295

Start: 484897

End: 485727

Strand: Direct

Name: nucI [H]

Synonym: Hore_04500

Alternate gene names: 220931295

Gene position: 484897-485727 (Clockwise)

Preceding gene: 220931292

Following gene: 220931296

Centisome position: 18.81

GC content: 40.55

Gene sequence:

>831_bases
ATGCTTAAAAAAACAACATCAATTATAACGGCCTTAATCTCTTTATTATTTTTATTGATGGCGGGTAACGTGATAAAAAA
ACATTATATACAGATACCGGGTATCAACCACGACCAAAAGCTTGAGAAAGTCTATGTAACCAGAGTTTATGATGGTGATA
CCTTCAGAACTGCCGATGGTGAAAATGTCAGGCTTATTGGTGTCGATACCCCGGAAATTAATCATGAAGAGGGAACAGCA
GAGTATTACGGGCGGGAAGCCCTGGCATATGCCCGCAAACACCTGGAAGGTAAGTATGTTTACCTGGAGTATGATCAGGA
CAGGCGGGATAAGTATGGCAGGCTACTGGCCTACGTCTTTCTTACCGATGGTATTTTCTTTAACGCCAGCTTACTGGAAG
AGGGTTATGCCCATTTAATTACCGTTCCCCCGAATATAAAGTATTTATCCCTTTTTAAAAAGACCGTTAAAAAGGCGAGG
GGGGAGGGGGCTGGAATATGGTCCCGGCTTAAAGTAAAAGAGGCCAGCCTTCCAGTTATTGGTTGGGAGGAAGCAGAAAA
CTATATAGGTAAAGAAGTTATTGTCCGGGGACAGATAGTGGACACCTATAATTCTGGTCAGGCCCTTTTCCTTAATTTTG
ACAGGGAGTACTGGAGGACATTCCGGGTAGTTATCTTTAGTGAAGATTTAAATAAATTCGGTTTTAACCCGGAGGAATAC
TTTGATAACAAATGTATTAAAGTTATGGGAAAGGTAAAGGAGTATGAAGGTAAACCTGAGATTATTGTGGATAGTCCTGA
AGAAATAATGGTTATAAAAAACTGTTTATAG

Upstream 100 bases:

>100_bases
CTGGGTTAATTATACCTAAACCCTGTGTCCGACAAATGGAGGGAAGGTCATATAACAATTTTTTAATTTTTTATATCTAA
ATTATTTGTGGGGTAACCTT

Downstream 100 bases:

>100_bases
AGTAAGGTGACCCTGTGTAAAAGAGAAGGTTTTATTTTATAAGGTAATACAATATGCCAGTAACCTTAATCTTGGTATAT
GGTAACATCTGACACATATT

Product: nuclease

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLKKTTSIITALISLLFLLMAGNVIKKHYIQIPGINHDQKLEKVYVTRVYDGDTFRTADGENVRLIGVDTPEINHEEGTA
EYYGREALAYARKHLEGKYVYLEYDQDRRDKYGRLLAYVFLTDGIFFNASLLEEGYAHLITVPPNIKYLSLFKKTVKKAR
GEGAGIWSRLKVKEASLPVIGWEEAENYIGKEVIVRGQIVDTYNSGQALFLNFDREYWRTFRVVIFSEDLNKFGFNPEEY
FDNKCIKVMGKVKEYEGKPEIIVDSPEEIMVIKNCL

Sequences:

>Translated_276_residues
MLKKTTSIITALISLLFLLMAGNVIKKHYIQIPGINHDQKLEKVYVTRVYDGDTFRTADGENVRLIGVDTPEINHEEGTA
EYYGREALAYARKHLEGKYVYLEYDQDRRDKYGRLLAYVFLTDGIFFNASLLEEGYAHLITVPPNIKYLSLFKKTVKKAR
GEGAGIWSRLKVKEASLPVIGWEEAENYIGKEVIVRGQIVDTYNSGQALFLNFDREYWRTFRVVIFSEDLNKFGFNPEEY
FDNKCIKVMGKVKEYEGKPEIIVDSPEEIMVIKNCL
>Mature_276_residues
MLKKTTSIITALISLLFLLMAGNVIKKHYIQIPGINHDQKLEKVYVTRVYDGDTFRTADGENVRLIGVDTPEINHEEGTA
EYYGREALAYARKHLEGKYVYLEYDQDRRDKYGRLLAYVFLTDGIFFNASLLEEGYAHLITVPPNIKYLSLFKKTVKKAR
GEGAGIWSRLKVKEASLPVIGWEEAENYIGKEVIVRGQIVDTYNSGQALFLNFDREYWRTFRVVIFSEDLNKFGFNPEEY
FDNKCIKVMGKVKEYEGKPEIIVDSPEEIMVIKNCL

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5'-position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 31852; Mature: 31852

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS01284 TNASE_2 ; PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTTSIITALISLLFLLMAGNVIKKHYIQIPGINHDQKLEKVYVTRVYDGDTFRTADG
CCCHHHHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCHHHHHEEEEEEEECCCEEECCCC
ENVRLIGVDTPEINHEEGTAEYYGREALAYARKHLEGKYVYLEYDQDRRDKYGRLLAYVF
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEE
LTDGIFFNASLLEEGYAHLITVPPNIKYLSLFKKTVKKARGEGAGIWSRLKVKEASLPVI
ECCCCEEEHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCEE
GWEEAENYIGKEVIVRGQIVDTYNSGQALFLNFDREYWRTFRVVIFSEDLNKFGFNPEEY
EHHHHHHHCCCEEEEEEEEEEEECCCCEEEEECCHHHHEEEEEEEEECCHHHHCCCHHHH
FDNKCIKVMGKVKEYEGKPEIIVDSPEEIMVIKNCL
HCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCC
>Mature Secondary Structure
MLKKTTSIITALISLLFLLMAGNVIKKHYIQIPGINHDQKLEKVYVTRVYDGDTFRTADG
CCCHHHHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCHHHHHEEEEEEEECCCEEECCCC
ENVRLIGVDTPEINHEEGTAEYYGREALAYARKHLEGKYVYLEYDQDRRDKYGRLLAYVF
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEE
LTDGIFFNASLLEEGYAHLITVPPNIKYLSLFKKTVKKARGEGAGIWSRLKVKEASLPVI
ECCCCEEEHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCEE
GWEEAENYIGKEVIVRGQIVDTYNSGQALFLNFDREYWRTFRVVIFSEDLNKFGFNPEEY
EHHHHHHHCCCEEEEEEEEEEEECCCCEEEEECCHHHHEEEEEEEEECCHHHHCCCHHHH
FDNKCIKVMGKVKEYEGKPEIIVDSPEEIMVIKNCL
HCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1408843 [H]