Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 220931270

GI number: 220931270

Start: 454473

End: 455486

Strand: Direct

Name: purR [H]

Synonym: Hore_04250

Alternate gene names: 220931270

Gene position: 454473-455486 (Clockwise)

Preceding gene: 220931269

Following gene: 220931271

Centisome position: 17.63

GC content: 36.0

Gene sequence:

>1014_bases
ATGGTTACCATTAAAGATATAGCAAGAATAGCAGGAGTATCAACAGCAACAGTCTCCAGGGTAATAAATAATTACCCACA
AGTGAATGAAAAAACAAAGAAAAAAGTCCTAGAAGTAATGAAGGAGAATAATTACAGGCCAAATTCAGTTGCCAGGAGTT
TATCGACCAGTAGATCATATACTATAGGTATCTTTTTTACAGATCACTTTGACACTGGTCTACGGCATCCCTTTTTTCGT
GAGGTTATTTACGGTTTAGAGAAAATATTTGGACAAAAGGGATATGATATATTATATTTTACCAAAAGAAACTGGGATGA
TAAATGTAGTTATGTTGACAAGTGCAGGGATAGACATGTTGATGGTGTAGTCCTGATGGGAGTATCCAGAGAAGATACAA
ATCTTCCCCAACTACTGGATTCAGGAATCCCTACAGTATTTATCGATGTGGACATTATTGGTAAAAGGGCTTCTTATGTT
ACTCTCAATAATACTGATGGGGCAAAAATGGCTGTTAATTATCTTTACAGTTTAGGTCACACTAAAATAGGTATGATTAT
GGGTATCAGTTCAACCAAGATTGCTCATGATAGATTTCTCGGGTATCAGATGGCTTTAAAAGATTTAAGTTTATCATATA
ATCCGGACTGGGTATTAAATGGTTTGTTTTCTGAAGAAGGTGGCTACCGGGCTATGAATAAATTTCTGAAAATGATAGAT
AGACCGACGGCAATCTTCTGCCAGAGTGATACCATGGCCATAGGTGCAATGAAAGCCATTAAAGAAGCAAAGATGAACGT
TCCTGGTGATTTCTCAATCATAGGTTTTGATGATATTGAAATTAGTAAATATGTAAAACCGGCTTTGACTACCATAAGAC
AGGATAAAGTAAAGTTGGGCAGGGCTGCAGGGAAACTTCTGTTAAATATTATTAATAATGACCTGGATGGTTATAAGCCC
GTAATTCTACCTGTTAAATTGATAAAGCGTAAATCCTGTGCTAAATTGAGATGA

Upstream 100 bases:

>100_bases
AAATTAAAATAAAGAAGGAATTTAAAAAACTATCGCGTAATTATATAATAGAGGGTACCGAAAACGGTTACAATTAAATA
AAGTAAATGGGGGACTGAAT

Downstream 100 bases:

>100_bases
TTTATTTTATGCAATAATGTAATCGATTTCGATATTAGAAATATTTGTTTAATTAAAAATTTTACAGTTATATCTTTAAA
TTGAATTATTCTAATTAAAT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MVTIKDIARIAGVSTATVSRVINNYPQVNEKTKKKVLEVMKENNYRPNSVARSLSTSRSYTIGIFFTDHFDTGLRHPFFR
EVIYGLEKIFGQKGYDILYFTKRNWDDKCSYVDKCRDRHVDGVVLMGVSREDTNLPQLLDSGIPTVFIDVDIIGKRASYV
TLNNTDGAKMAVNYLYSLGHTKIGMIMGISSTKIAHDRFLGYQMALKDLSLSYNPDWVLNGLFSEEGGYRAMNKFLKMID
RPTAIFCQSDTMAIGAMKAIKEAKMNVPGDFSIIGFDDIEISKYVKPALTTIRQDKVKLGRAAGKLLLNIINNDLDGYKP
VILPVKLIKRKSCAKLR

Sequences:

>Translated_337_residues
MVTIKDIARIAGVSTATVSRVINNYPQVNEKTKKKVLEVMKENNYRPNSVARSLSTSRSYTIGIFFTDHFDTGLRHPFFR
EVIYGLEKIFGQKGYDILYFTKRNWDDKCSYVDKCRDRHVDGVVLMGVSREDTNLPQLLDSGIPTVFIDVDIIGKRASYV
TLNNTDGAKMAVNYLYSLGHTKIGMIMGISSTKIAHDRFLGYQMALKDLSLSYNPDWVLNGLFSEEGGYRAMNKFLKMID
RPTAIFCQSDTMAIGAMKAIKEAKMNVPGDFSIIGFDDIEISKYVKPALTTIRQDKVKLGRAAGKLLLNIINNDLDGYKP
VILPVKLIKRKSCAKLR
>Mature_337_residues
MVTIKDIARIAGVSTATVSRVINNYPQVNEKTKKKVLEVMKENNYRPNSVARSLSTSRSYTIGIFFTDHFDTGLRHPFFR
EVIYGLEKIFGQKGYDILYFTKRNWDDKCSYVDKCRDRHVDGVVLMGVSREDTNLPQLLDSGIPTVFIDVDIIGKRASYV
TLNNTDGAKMAVNYLYSLGHTKIGMIMGISSTKIAHDRFLGYQMALKDLSLSYNPDWVLNGLFSEEGGYRAMNKFLKMID
RPTAIFCQSDTMAIGAMKAIKEAKMNVPGDFSIIGFDDIEISKYVKPALTTIRQDKVKLGRAAGKLLLNIINNDLDGYKP
VILPVKLIKRKSCAKLR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=35.9050445103858, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790369, Length=351, Percent_Identity=34.1880341880342, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1790194, Length=338, Percent_Identity=32.8402366863905, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=35.0340136054422, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1788474, Length=345, Percent_Identity=32.463768115942, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=30.791788856305, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=27.7611940298507, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.3492063492064, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790715, Length=329, Percent_Identity=27.6595744680851, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789456, Length=300, Percent_Identity=28.3333333333333, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787906, Length=355, Percent_Identity=24.7887323943662, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790689, Length=333, Percent_Identity=24.9249249249249, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=318, Percent_Identity=23.8993710691824, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=22.9813664596273, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37984; Mature: 37984

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTIKDIARIAGVSTATVSRVINNYPQVNEKTKKKVLEVMKENNYRPNSVARSLSTSRSY
CCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGIFFTDHFDTGLRHPFFREVIYGLEKIFGQKGYDILYFTKRNWDDKCSYVDKCRDRHV
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
DGVVLMGVSREDTNLPQLLDSGIPTVFIDVDIIGKRASYVTLNNTDGAKMAVNYLYSLGH
CCEEEEECCCCCCCCHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCC
TKIGMIMGISSTKIAHDRFLGYQMALKDLSLSYNPDWVLNGLFSEEGGYRAMNKFLKMID
CEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHCCCCCHHHHHHHHHHHC
RPTAIFCQSDTMAIGAMKAIKEAKMNVPGDFSIIGFDDIEISKYVKPALTTIRQDKVKLG
CCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RAAGKLLLNIINNDLDGYKPVILPVKLIKRKSCAKLR
HHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCC
>Mature Secondary Structure
MVTIKDIARIAGVSTATVSRVINNYPQVNEKTKKKVLEVMKENNYRPNSVARSLSTSRSY
CCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGIFFTDHFDTGLRHPFFREVIYGLEKIFGQKGYDILYFTKRNWDDKCSYVDKCRDRHV
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
DGVVLMGVSREDTNLPQLLDSGIPTVFIDVDIIGKRASYVTLNNTDGAKMAVNYLYSLGH
CCEEEEECCCCCCCCHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCC
TKIGMIMGISSTKIAHDRFLGYQMALKDLSLSYNPDWVLNGLFSEEGGYRAMNKFLKMID
CEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHCCCCCHHHHHHHHHHHC
RPTAIFCQSDTMAIGAMKAIKEAKMNVPGDFSIIGFDDIEISKYVKPALTTIRQDKVKLG
CCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RAAGKLLLNIINNDLDGYKPVILPVKLIKRKSCAKLR
HHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA