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The map label for this gene is hyaB [C]

Identifier: 220931232

GI number: 220931232

Start: 397225

End: 398637

Strand: Direct

Name: hyaB [C]

Synonym: Hore_03860

Alternate gene names: 220931232

Gene position: 397225-398637 (Clockwise)

Preceding gene: 220931231

Following gene: 220931233

Centisome position: 15.41

GC content: 41.97

Gene sequence:

>1413_bases
ATGGTTAAAAAAATTACATTTAGTCCGGTAACAAGGTTAAGTGGACTTTTGTCAGTTGAAATTGTTGTTGATAGAGGGAA
AATAGTGAATGCCCGTACAAGAGGAACCATGTTCAGAGGTTATGAGTGGATTATGCGAGGAAGAAAGGTAACAGATGCTG
TTTATATGACCCAGAGGATCTGTGGTATTTGTTCCCTGGCCCATGGGGCTGTTGGAAGTTATTTGCTTGATGAACTTTAT
GGGAATATCATATCTGAAAATGCTCAGTATCTAAGAAATATTATGTTTGCGGCAGATTTTTTACAAAACCATATCAGGCA
TTTTTATTTTTTTGGCCTTCCCGATTTTGTTAAAATGCCTGAAAAACCACCTTTTCTGGGGCAGGACCTTAATGACCTTC
GTTTAAATAGGGAAGACAATCGACGACTGGTTGATAATTATTACAAGGCTGTTAAAGCAGCTCAGGAAAGTCATCAGTTA
CTGGCCCTGTTTGGTGGCAAGATACCGAACCAGCATAGTTTTGTCCACGGAGGGGTAACGGTAGCCCCGACCGCTGACAA
GATTAACCAGGCAAAAGCTCTAATTGAAAGTATTCAACAATTTGTTCAGGGGTGTATGATACCGGATACTGAATTAATTT
CCCGGGCTTACAGGGATTATTTTCAGATCGGTGTTACCCCTCTTCGACTAATATCTTTTGGTCTTTTTCCTTTTGGTCCA
CAAAATGACGATTATTTATGGCCCCCCGGTGTCATTATCAATAATGAATTTGAGCTACCTCAGGTAGAATTTATTGAAGA
ACAGGTACCTTACTCCTATTATGAGGCGAATGATGAAGGCGAAAATGTATTCAATCAATACCCGGAACCTGCTCCCTTTA
AACCAGAAGCCTATAGTTATATAAAAACTGTTCTGTATAAAGGCCAACACTTTGAAACAGGGCCATATGCCAGGGGAGTT
ATCCGTGGAGATTATAGGGGAAGGGCTTCGACCATGGATAGAATTGTGGCCCGTTCCCTGGAGGCATTGAAGCTGGCTGG
TTTTATCAGGGAATGGTTGACCAGACTGGAACCTGGACCTCCACCTTTAAATCAAAATGAAGAACCGGTTAAAGATAGGG
CTGTGGCTACTACTGGAGCTATGCGGGGAGCCCTTCTACACAGTGCTATAATCGAAGGTGAAGAAGTAAAAAGTTATAAT
GTCATCACCCCAACCACCTGGAATTTCTCACCGAGGGATAAAAATGGGAATCCTGGTCCGGTAGAAAGGGCCTTAATTGG
TACTGAAATTCCCGGGGGAGCAATGTTGGAGACCATTGTTGGCAGGATAATTCGGTCCTTTGATCCCTGTATATCCTGTG
GTACCCATGTCTATAGTATTGACGGTAAATTGCAGAAGAAAATAGAATTTTGA

Upstream 100 bases:

>100_bases
CCCTTGAAAAGGCAAGAAGATGAAGAAGTAACCCCTGGAAGGGATGGGGATAAGATAAATGGGTTTAAAGATAGTAAAGA
TACCTGTGGGGATGATACTG

Downstream 100 bases:

>100_bases
AAGTAGTGGTTAAATTGCATGAAATGTCTGTCATGGGGGAGATTTTTTCAATAATAAACAGTAATGTAGCTAAATACAGA
CTTAAAAGAGTAACAAAAGT

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVKKITFSPVTRLSGLLSVEIVVDRGKIVNARTRGTMFRGYEWIMRGRKVTDAVYMTQRICGICSLAHGAVGSYLLDELY
GNIISENAQYLRNIMFAADFLQNHIRHFYFFGLPDFVKMPEKPPFLGQDLNDLRLNREDNRRLVDNYYKAVKAAQESHQL
LALFGGKIPNQHSFVHGGVTVAPTADKINQAKALIESIQQFVQGCMIPDTELISRAYRDYFQIGVTPLRLISFGLFPFGP
QNDDYLWPPGVIINNEFELPQVEFIEEQVPYSYYEANDEGENVFNQYPEPAPFKPEAYSYIKTVLYKGQHFETGPYARGV
IRGDYRGRASTMDRIVARSLEALKLAGFIREWLTRLEPGPPPLNQNEEPVKDRAVATTGAMRGALLHSAIIEGEEVKSYN
VITPTTWNFSPRDKNGNPGPVERALIGTEIPGGAMLETIVGRIIRSFDPCISCGTHVYSIDGKLQKKIEF

Sequences:

>Translated_470_residues
MVKKITFSPVTRLSGLLSVEIVVDRGKIVNARTRGTMFRGYEWIMRGRKVTDAVYMTQRICGICSLAHGAVGSYLLDELY
GNIISENAQYLRNIMFAADFLQNHIRHFYFFGLPDFVKMPEKPPFLGQDLNDLRLNREDNRRLVDNYYKAVKAAQESHQL
LALFGGKIPNQHSFVHGGVTVAPTADKINQAKALIESIQQFVQGCMIPDTELISRAYRDYFQIGVTPLRLISFGLFPFGP
QNDDYLWPPGVIINNEFELPQVEFIEEQVPYSYYEANDEGENVFNQYPEPAPFKPEAYSYIKTVLYKGQHFETGPYARGV
IRGDYRGRASTMDRIVARSLEALKLAGFIREWLTRLEPGPPPLNQNEEPVKDRAVATTGAMRGALLHSAIIEGEEVKSYN
VITPTTWNFSPRDKNGNPGPVERALIGTEIPGGAMLETIVGRIIRSFDPCISCGTHVYSIDGKLQKKIEF
>Mature_470_residues
MVKKITFSPVTRLSGLLSVEIVVDRGKIVNARTRGTMFRGYEWIMRGRKVTDAVYMTQRICGICSLAHGAVGSYLLDELY
GNIISENAQYLRNIMFAADFLQNHIRHFYFFGLPDFVKMPEKPPFLGQDLNDLRLNREDNRRLVDNYYKAVKAAQESHQL
LALFGGKIPNQHSFVHGGVTVAPTADKINQAKALIESIQQFVQGCMIPDTELISRAYRDYFQIGVTPLRLISFGLFPFGP
QNDDYLWPPGVIINNEFELPQVEFIEEQVPYSYYEANDEGENVFNQYPEPAPFKPEAYSYIKTVLYKGQHFETGPYARGV
IRGDYRGRASTMDRIVARSLEALKLAGFIREWLTRLEPGPPPLNQNEEPVKDRAVATTGAMRGALLHSAIIEGEEVKSYN
VITPTTWNFSPRDKNGNPGPVERALIGTEIPGGAMLETIVGRIIRSFDPCISCGTHVYSIDGKLQKKIEF

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=577, Percent_Identity=28.42287694974, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789368, Length=572, Percent_Identity=25.8741258741259, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 53061; Mature: 53061

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKITFSPVTRLSGLLSVEIVVDRGKIVNARTRGTMFRGYEWIMRGRKVTDAVYMTQRI
CCCEECCCHHHHHCCCEEEEEEEECCCEEECHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
CGICSLAHGAVGSYLLDELYGNIISENAQYLRNIMFAADFLQNHIRHFYFFGLPDFVKMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHCCC
EKPPFLGQDLNDLRLNREDNRRLVDNYYKAVKAAQESHQLLALFGGKIPNQHSFVHGGVT
CCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEE
VAPTADKINQAKALIESIQQFVQGCMIPDTELISRAYRDYFQIGVTPLRLISFGLFPFGP
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
QNDDYLWPPGVIINNEFELPQVEFIEEQVPYSYYEANDEGENVFNQYPEPAPFKPEAYSY
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHCCCCCCCCCCHHHHH
IKTVLYKGQHFETGPYARGVIRGDYRGRASTMDRIVARSLEALKLAGFIREWLTRLEPGP
HHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PPLNQNEEPVKDRAVATTGAMRGALLHSAIIEGEEVKSYNVITPTTWNFSPRDKNGNPGP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VERALIGTEIPGGAMLETIVGRIIRSFDPCISCGTHVYSIDGKLQKKIEF
HHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEEEECCHHHHHCCC
>Mature Secondary Structure
MVKKITFSPVTRLSGLLSVEIVVDRGKIVNARTRGTMFRGYEWIMRGRKVTDAVYMTQRI
CCCEECCCHHHHHCCCEEEEEEEECCCEEECHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
CGICSLAHGAVGSYLLDELYGNIISENAQYLRNIMFAADFLQNHIRHFYFFGLPDFVKMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHCCC
EKPPFLGQDLNDLRLNREDNRRLVDNYYKAVKAAQESHQLLALFGGKIPNQHSFVHGGVT
CCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEE
VAPTADKINQAKALIESIQQFVQGCMIPDTELISRAYRDYFQIGVTPLRLISFGLFPFGP
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
QNDDYLWPPGVIINNEFELPQVEFIEEQVPYSYYEANDEGENVFNQYPEPAPFKPEAYSY
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHCCCCCCCCCCHHHHH
IKTVLYKGQHFETGPYARGVIRGDYRGRASTMDRIVARSLEALKLAGFIREWLTRLEPGP
HHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PPLNQNEEPVKDRAVATTGAMRGALLHSAIIEGEEVKSYNVITPTTWNFSPRDKNGNPGP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VERALIGTEIPGGAMLETIVGRIIRSFDPCISCGTHVYSIDGKLQKKIEF
HHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]