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The map label for this gene is hyb0 [C]

Identifier: 220931231

GI number: 220931231

Start: 396228

End: 397232

Strand: Direct

Name: hyb0 [C]

Synonym: Hore_03850

Alternate gene names: 220931231

Gene position: 396228-397232 (Clockwise)

Preceding gene: 220931229

Following gene: 220931232

Centisome position: 15.37

GC content: 41.0

Gene sequence:

>1005_bases
GTGAAGAAAAAAGACTATGTTAATCTCTGTCGTTCTTTAAAGACTGAGGGGAGTCATCAATTATCAAGAGAAATTTTAAG
AGTGGTCAGGGACTTTCGGAGTAACAATGGCAAAAAATTACCTGTTATCTGGATTGAAACCAACGATAGTGGTGATAATA
ATATTTCTTTTATGAACACAACCTATCCCTATCTTGATCAGGTTTTTGAAGATATTATAGATCTTTATTATAGTAATACC
TTTATGGCAGCCCAGGGAGAGGATGCCTATCAAATATTAAAAAAAGTGGCTTTACGCGAAAAAAGAGAGTTTACCCTTGT
TGTTGAGGGGGCTATTCCTCTGAAAGCAAACGGTCGGTATAATATTACCTTTATTACTGATGAAGGTCCGGTAACAGCAG
TTGAGTTAGTAAGATGGCTTGGAGAGCTGGCAAAATATGCAGTGGCGGTAGGAACCTGTGCCAGTTTTGGTGGAGCATCA
GCAGCCTCTCCAAATATTACCGGTAGTGTAGCTCTTGGAGATGTATTAGACCGGCAAATAATAAATGTAAGTGGTTGCCC
CATAAACCCTGATTGGTTTGTTGGTACACTGGCTCATCTACAGATGTACGGTCTACCGGATCTGGATAGACTGGGGAGGC
CTGAACTTTTTTATCAGTTTACTGTTCACCGTCATTGCCAACGGCGTTCCTATTTTGATGATGGAAATTTCGCTGAAGAG
CTGGGAGACATTGAATGTATGTTCAGTCAGGGTTGTGTGGGCCCCAGAACAGGTGCTGACTGTCCTTACCGTTTATGGGA
TAATCATGTTAGTTATCCTATCAAAGTTAATACACCCTGTATAGGTTGTACTAATGAAGATTTCCCCGATGGGTCTTCAC
CGTTTTTTGTCCCCTTACCCTTGAAAAGGCAAGAAGATGAAGAAGTAACCCCTGGAAGGGATGGGGATAAGATAAATGGG
TTTAAAGATAGTAAAGATACCTGTGGGGATGATACTGATGGTTAA

Upstream 100 bases:

>100_bases
TAATAATTTGATATTTACACTTATTTCCTCCCATATTATCATTAGTATGCAAGTTAAAGCATAATATAACTTACAAAGGA
ATTAAATGAGGTGTATTATT

Downstream 100 bases:

>100_bases
AAAAATTACATTTAGTCCGGTAACAAGGTTAAGTGGACTTTTGTCAGTTGAAATTGTTGTTGATAGAGGGAAAATAGTGA
ATGCCCGTACAAGAGGAACC

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKKKDYVNLCRSLKTEGSHQLSREILRVVRDFRSNNGKKLPVIWIETNDSGDNNISFMNTTYPYLDQVFEDIIDLYYSNT
FMAAQGEDAYQILKKVALREKREFTLVVEGAIPLKANGRYNITFITDEGPVTAVELVRWLGELAKYAVAVGTCASFGGAS
AASPNITGSVALGDVLDRQIINVSGCPINPDWFVGTLAHLQMYGLPDLDRLGRPELFYQFTVHRHCQRRSYFDDGNFAEE
LGDIECMFSQGCVGPRTGADCPYRLWDNHVSYPIKVNTPCIGCTNEDFPDGSSPFFVPLPLKRQEDEEVTPGRDGDKING
FKDSKDTCGDDTDG

Sequences:

>Translated_334_residues
MKKKDYVNLCRSLKTEGSHQLSREILRVVRDFRSNNGKKLPVIWIETNDSGDNNISFMNTTYPYLDQVFEDIIDLYYSNT
FMAAQGEDAYQILKKVALREKREFTLVVEGAIPLKANGRYNITFITDEGPVTAVELVRWLGELAKYAVAVGTCASFGGAS
AASPNITGSVALGDVLDRQIINVSGCPINPDWFVGTLAHLQMYGLPDLDRLGRPELFYQFTVHRHCQRRSYFDDGNFAEE
LGDIECMFSQGCVGPRTGADCPYRLWDNHVSYPIKVNTPCIGCTNEDFPDGSSPFFVPLPLKRQEDEEVTPGRDGDKING
FKDSKDTCGDDTDG
>Mature_334_residues
MKKKDYVNLCRSLKTEGSHQLSREILRVVRDFRSNNGKKLPVIWIETNDSGDNNISFMNTTYPYLDQVFEDIIDLYYSNT
FMAAQGEDAYQILKKVALREKREFTLVVEGAIPLKANGRYNITFITDEGPVTAVELVRWLGELAKYAVAVGTCASFGGAS
AASPNITGSVALGDVLDRQIINVSGCPINPDWFVGTLAHLQMYGLPDLDRLGRPELFYQFTVHRHCQRRSYFDDGNFAEE
LGDIECMFSQGCVGPRTGADCPYRLWDNHVSYPIKVNTPCIGCTNEDFPDGSSPFFVPLPLKRQEDEEVTPGRDGDKING
FKDSKDTCGDDTDG

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=296, Percent_Identity=34.4594594594595, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787206, Length=265, Percent_Identity=33.2075471698113, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 37333; Mature: 37333

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKDYVNLCRSLKTEGSHQLSREILRVVRDFRSNNGKKLPVIWIETNDSGDNNISFMNT
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEC
TYPYLDQVFEDIIDLYYSNTFMAAQGEDAYQILKKVALREKREFTLVVEGAIPLKANGRY
CHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCEE
NITFITDEGPVTAVELVRWLGELAKYAVAVGTCASFGGASAASPNITGSVALGDVLDRQI
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
INVSGCPINPDWFVGTLAHLQMYGLPDLDRLGRPELFYQFTVHRHCQRRSYFDDGNFAEE
EECCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHH
LGDIECMFSQGCVGPRTGADCPYRLWDNHVSYPIKVNTPCIGCTNEDFPDGSSPFFVPLP
HCCEEEEHHCCCCCCCCCCCCCHHHHCCCCCCEEEECCCEEECCCCCCCCCCCCEEEECC
LKRQEDEEVTPGRDGDKINGFKDSKDTCGDDTDG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKKDYVNLCRSLKTEGSHQLSREILRVVRDFRSNNGKKLPVIWIETNDSGDNNISFMNT
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEC
TYPYLDQVFEDIIDLYYSNTFMAAQGEDAYQILKKVALREKREFTLVVEGAIPLKANGRY
CHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCEE
NITFITDEGPVTAVELVRWLGELAKYAVAVGTCASFGGASAASPNITGSVALGDVLDRQI
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
INVSGCPINPDWFVGTLAHLQMYGLPDLDRLGRPELFYQFTVHRHCQRRSYFDDGNFAEE
EECCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHH
LGDIECMFSQGCVGPRTGADCPYRLWDNHVSYPIKVNTPCIGCTNEDFPDGSSPFFVPLP
HCCEEEEHHCCCCCCCCCCCCCHHHHCCCCCCEEEECCCEEECCCCCCCCCCCCEEEECC
LKRQEDEEVTPGRDGDKINGFKDSKDTCGDDTDG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]