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The map label for this gene is ansB [H]

Identifier: 220931163

GI number: 220931163

Start: 324807

End: 326222

Strand: Direct

Name: ansB [H]

Synonym: Hore_03150

Alternate gene names: 220931163

Gene position: 324807-326222 (Clockwise)

Preceding gene: 220931162

Following gene: 220931164

Centisome position: 12.6

GC content: 41.38

Gene sequence:

>1416_bases
ATGTTAACCAGAAAAGAAAGGGATCCACTAGGTGAAAAGGAAGTACCCAGGGATGCATATTACGGAATTCAGACCCTGAG
GGCAGCTGAAAATTTTCAAATTACCAATTTGAGGATTCACCCGGAATTAATAAAATCTCTTGCCTATGTAAAAAAGGCAG
CAGCTTCTGTAAATATTGAAATAGGTATGTTGAATGAGAAGATAGGTAAGGCTATTATTCAGGCTACAGATGAAGTAATT
AATGGCTCCCTTAATGAAGAGTTTATAGTTGATGCCCTCCAGGGCGGGGCCGGCACTTCTATAAATATGAATATGAATGA
GGTGCTCGCCAACCGGGCTATAGAAATTTTAGGTGGAGAAAAAGGTGATTATAGTCTGGTTCACCCCAACAACCATGTAA
ATATGGGGCAATCTACCAATGATGTTTATCCTACTGCAATAAGGATTACCGCAATAAAGCTTTTGAGAAGGGCACTTGAA
GAATTATCATCAATGTATGGGGCCCTTACCAGAAAATCGGGGGAATTTGACCATATTATTAAAATGGGGCGGACTCAGTT
ACAGGATGCAGTCCCCATCAGGCTGGGTCAGGAATTTGGGGCCTATGCCAGTGCCATTAAAAGGGATATAAGGAGGATTA
AAGAAACAGTAGCTGATCTAAAGGTGGTAAATCTGGGGGCCACTGCCATCGGTACTGGTTTGAATGCCGATCATGAATAT
GTTAACAATGTAACCAGACGTTTAAATGAGTTAACCGGGCTTGATTTAACCCAGGCAGATGACCTTATCGATGCTACCCA
GAATGCAGATGCCCTGGTTGAAGTTTCCTCAGCTCTTAAGACCTGTGCAGTTAATCTTTCCAAGATAGCCAGTGACTTAC
GTTTATTATCTTCAGGGCCCCGGGCCGGATTGAACGAAATAAACCTGCCGGCTGTACCGCCCGGGTCTTCAATTATGCCG
GGGAAGGTAAACCCTGTTATTCCGGAAGTTGTAAATCAAATTGCCTATCAGATAATCGGTAATGATACCACTATAACTAT
GGCTGCAGAGTCAGGTCAGCTGGAGTTAAATGTTATGATCCCGGTTTTAACATTTAACCTTTTCCAATCAATAGAGATAT
TGAAAAATGGAGTAAAAACCTTTACTGAAAAAGCCATTGAGGGAATTACTGTTAATGAAGAAAGGTGTAAAGAACTTGTG
GATAAGAGTGTCAGTATCATTACTGCAATTAATCCCCATGTTGGTTATGAAGTTGCATCCCGGATTGCTAAAAAGGCTTT
AAAAACCGGGAAGTCGGTTAAAGAATTAATCCTGGAAGAAGGGGTGCTTAATGAAGAAGAACTTAAATTGATATTAAACC
CCTATGAGATGACAGAACCCGGTATTGCCGGCAAGGAGTTACTTGAAGGGCATTAA

Upstream 100 bases:

>100_bases
GGGAGCCTTCTTACAGAAGGCAAAAAAACTTGTTGGGATAAATAATGTAAAGATATGGTCATCATAAATTAATTAAAACA
GGCAGGGGGAGAATATAATT

Downstream 100 bases:

>100_bases
TTTACTGGTAAAAAACTGGAGGTTATAGGTTATGAATGAGGTATTTTTATGGCTTGATAAATATTTATCCCGGGAACTTG
CTGTATTAATTACAGCCTGT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLTRKERDPLGEKEVPRDAYYGIQTLRAAENFQITNLRIHPELIKSLAYVKKAAASVNIEIGMLNEKIGKAIIQATDEVI
NGSLNEEFIVDALQGGAGTSINMNMNEVLANRAIEILGGEKGDYSLVHPNNHVNMGQSTNDVYPTAIRITAIKLLRRALE
ELSSMYGALTRKSGEFDHIIKMGRTQLQDAVPIRLGQEFGAYASAIKRDIRRIKETVADLKVVNLGATAIGTGLNADHEY
VNNVTRRLNELTGLDLTQADDLIDATQNADALVEVSSALKTCAVNLSKIASDLRLLSSGPRAGLNEINLPAVPPGSSIMP
GKVNPVIPEVVNQIAYQIIGNDTTITMAAESGQLELNVMIPVLTFNLFQSIEILKNGVKTFTEKAIEGITVNEERCKELV
DKSVSIITAINPHVGYEVASRIAKKALKTGKSVKELILEEGVLNEEELKLILNPYEMTEPGIAGKELLEGH

Sequences:

>Translated_471_residues
MLTRKERDPLGEKEVPRDAYYGIQTLRAAENFQITNLRIHPELIKSLAYVKKAAASVNIEIGMLNEKIGKAIIQATDEVI
NGSLNEEFIVDALQGGAGTSINMNMNEVLANRAIEILGGEKGDYSLVHPNNHVNMGQSTNDVYPTAIRITAIKLLRRALE
ELSSMYGALTRKSGEFDHIIKMGRTQLQDAVPIRLGQEFGAYASAIKRDIRRIKETVADLKVVNLGATAIGTGLNADHEY
VNNVTRRLNELTGLDLTQADDLIDATQNADALVEVSSALKTCAVNLSKIASDLRLLSSGPRAGLNEINLPAVPPGSSIMP
GKVNPVIPEVVNQIAYQIIGNDTTITMAAESGQLELNVMIPVLTFNLFQSIEILKNGVKTFTEKAIEGITVNEERCKELV
DKSVSIITAINPHVGYEVASRIAKKALKTGKSVKELILEEGVLNEEELKLILNPYEMTEPGIAGKELLEGH
>Mature_471_residues
MLTRKERDPLGEKEVPRDAYYGIQTLRAAENFQITNLRIHPELIKSLAYVKKAAASVNIEIGMLNEKIGKAIIQATDEVI
NGSLNEEFIVDALQGGAGTSINMNMNEVLANRAIEILGGEKGDYSLVHPNNHVNMGQSTNDVYPTAIRITAIKLLRRALE
ELSSMYGALTRKSGEFDHIIKMGRTQLQDAVPIRLGQEFGAYASAIKRDIRRIKETVADLKVVNLGATAIGTGLNADHEY
VNNVTRRLNELTGLDLTQADDLIDATQNADALVEVSSALKTCAVNLSKIASDLRLLSSGPRAGLNEINLPAVPPGSSIMP
GKVNPVIPEVVNQIAYQIIGNDTTITMAAESGQLELNVMIPVLTFNLFQSIEILKNGVKTFTEKAIEGITVNEERCKELV
DKSVSIITAINPHVGYEVASRIAKKALKTGKSVKELILEEGVLNEEELKLILNPYEMTEPGIAGKELLEGH

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=44.7826086956522, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=51.3978494623656, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=42.7645788336933, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1790398, Length=318, Percent_Identity=23.5849056603774, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=44.7826086956522, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=40.9785932721713, Blast_Score=265, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=45.2173913043478, Blast_Score=399, Evalue=1e-112,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=43.2900432900433, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=42.9501084598698, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=42.9501084598698, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=41.9006479481641, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=38.6609071274298, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51266; Mature: 51266

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKERDPLGEKEVPRDAYYGIQTLRAAENFQITNLRIHPELIKSLAYVKKAAASVNIE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHEEEE
IGMLNEKIGKAIIQATDEVINGSLNEEFIVDALQGGAGTSINMNMNEVLANRAIEILGGE
EEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCC
KGDYSLVHPNNHVNMGQSTNDVYPTAIRITAIKLLRRALEELSSMYGALTRKSGEFDHII
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KMGRTQLQDAVPIRLGQEFGAYASAIKRDIRRIKETVADLKVVNLGATAIGTGLNADHEY
HHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHCCCCCCCHHH
VNNVTRRLNELTGLDLTQADDLIDATQNADALVEVSSALKTCAVNLSKIASDLRLLSSGP
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RAGLNEINLPAVPPGSSIMPGKVNPVIPEVVNQIAYQIIGNDTTITMAAESGQLELNVMI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEH
PVLTFNLFQSIEILKNGVKTFTEKAIEGITVNEERCKELVDKSVSIITAINPHVGYEVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHH
RIAKKALKTGKSVKELILEEGVLNEEELKLILNPYEMTEPGIAGKELLEGH
HHHHHHHHCCHHHHHHHHHCCCCCHHHHEEEECCHHCCCCCCCHHHHHCCC
>Mature Secondary Structure
MLTRKERDPLGEKEVPRDAYYGIQTLRAAENFQITNLRIHPELIKSLAYVKKAAASVNIE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHEEEE
IGMLNEKIGKAIIQATDEVINGSLNEEFIVDALQGGAGTSINMNMNEVLANRAIEILGGE
EEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCC
KGDYSLVHPNNHVNMGQSTNDVYPTAIRITAIKLLRRALEELSSMYGALTRKSGEFDHII
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KMGRTQLQDAVPIRLGQEFGAYASAIKRDIRRIKETVADLKVVNLGATAIGTGLNADHEY
HHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHCCCCCCCHHH
VNNVTRRLNELTGLDLTQADDLIDATQNADALVEVSSALKTCAVNLSKIASDLRLLSSGP
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RAGLNEINLPAVPPGSSIMPGKVNPVIPEVVNQIAYQIIGNDTTITMAAESGQLELNVMI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEH
PVLTFNLFQSIEILKNGVKTFTEKAIEGITVNEERCKELVDKSVSIITAINPHVGYEVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHH
RIAKKALKTGKSVKELILEEGVLNEEELKLILNPYEMTEPGIAGKELLEGH
HHHHHHHHCCHHHHHHHHHCCCCCHHHHEEEECCHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]