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The map label for this gene is sleB [H]

Identifier: 220931154

GI number: 220931154

Start: 315885

End: 316604

Strand: Direct

Name: sleB [H]

Synonym: Hore_03060

Alternate gene names: 220931154

Gene position: 315885-316604 (Clockwise)

Preceding gene: 220931153

Following gene: 220931155

Centisome position: 12.25

GC content: 46.81

Gene sequence:

>720_bases
ATGAAAGGGGTTGGCAGAGTGAAAAAAAAGATTATAACCCTTCTGGTAATTTTAGGTATTACCGGAATTTCTACTGTAGT
ATATTTCACTGCACTGAATACAAATACTGAAGCAGCACGACCTACTCTTTACTGGGGAACAAGTGGTAGTAATGTAAGGT
TGGTTCAATGGAAGCTACAACAATGGGGATATTATGAAGGGAGAATAGATGGTTATTTTGGTCCGGAGACTTCAGAAGCA
GTCAGGGAGTTTCAGAGGAAGAATGGGCTCAGGGTTGACGGACTCGTCGGTCCTCAGACCTGGGCAGCCCTTGGATATGA
AGCCCGTCCTACTACATATGCCAGGCAAACAGCGGCAGCAGTTAGCAGAAATGATGATGTCCAGCTACTGGCGAGGTTAG
TTCATGCTGAAGCAAGAGGGGAACCATACCGGGGACAGGTTGCGATTGCAGCAGTAGTCCTGAACAGGGTGGAAAGCCCC
TCATTTCCCAACAGTCTAAGTGGGGTTATATACCAGCCACTGGCTTTTGAATCAGTGGCCAATGGGCAGATCAATTTACC
ACCTACCCAGGAAAATTTACGGGCAGCAAGAGCAGCACTTAATGGATGGGATCCAACCTACGGTTGTTTATTTTTCTGGA
ATCCTTCAAAACCGGTTAGCCGGTGGATCTGGTCCCGTAGGGTAGTAACGACAATTGGTAGCCATGTATTTGCCCAGTAA

Upstream 100 bases:

>100_bases
TTGTTCTATTCGTTATTCCCCAATGTTAAAAATTGGATATTACAAAAGAATAAAAACAGGGGGGGCTGAGGCAACATAAG
TTAAAAAGGGTTATTAAAAT

Downstream 100 bases:

>100_bases
TAGAAATTAGCATGTAACGTTTCAAACTTAAATTTAATCTAAAGCTTATAAAGGAGGAATAAAAAATTAAGATTAATCGC
AACTGGATCATTCCTGTGTT

Product: Spore cortex-lytic enzyme SleB

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKGVGRVKKKIITLLVILGITGISTVVYFTALNTNTEAARPTLYWGTSGSNVRLVQWKLQQWGYYEGRIDGYFGPETSEA
VREFQRKNGLRVDGLVGPQTWAALGYEARPTTYARQTAAAVSRNDDVQLLARLVHAEARGEPYRGQVAIAAVVLNRVESP
SFPNSLSGVIYQPLAFESVANGQINLPPTQENLRAARAALNGWDPTYGCLFFWNPSKPVSRWIWSRRVVTTIGSHVFAQ

Sequences:

>Translated_239_residues
MKGVGRVKKKIITLLVILGITGISTVVYFTALNTNTEAARPTLYWGTSGSNVRLVQWKLQQWGYYEGRIDGYFGPETSEA
VREFQRKNGLRVDGLVGPQTWAALGYEARPTTYARQTAAAVSRNDDVQLLARLVHAEARGEPYRGQVAIAAVVLNRVESP
SFPNSLSGVIYQPLAFESVANGQINLPPTQENLRAARAALNGWDPTYGCLFFWNPSKPVSRWIWSRRVVTTIGSHVFAQ
>Mature_239_residues
MKGVGRVKKKIITLLVILGITGISTVVYFTALNTNTEAARPTLYWGTSGSNVRLVQWKLQQWGYYEGRIDGYFGPETSEA
VREFQRKNGLRVDGLVGPQTWAALGYEARPTTYARQTAAAVSRNDDVQLLARLVHAEARGEPYRGQVAIAAVVLNRVESP
SFPNSLSGVIYQPLAFESVANGQINLPPTQENLRAARAALNGWDPTYGCLFFWNPSKPVSRWIWSRRVVTTIGSHVFAQ

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: NA

Molecular weight: Translated: 26485; Mature: 26485

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVGRVKKKIITLLVILGITGISTVVYFTALNTNTEAARPTLYWGTSGSNVRLVQWKLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCCCCEEEEEEEHH
QWGYYEGRIDGYFGPETSEAVREFQRKNGLRVDGLVGPQTWAALGYEARPTTYARQTAAA
HCCCCCCEECCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHCCCCCCCCHHHHHHHHH
VSRNDDVQLLARLVHAEARGEPYRGQVAIAAVVLNRVESPSFPNSLSGVIYQPLAFESVA
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHEECCHHHHHHC
NGQINLPPTQENLRAARAALNGWDPTYGCLFFWNPSKPVSRWIWSRRVVTTIGSHVFAQ
CCEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGVGRVKKKIITLLVILGITGISTVVYFTALNTNTEAARPTLYWGTSGSNVRLVQWKLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCCCCEEEEEEEHH
QWGYYEGRIDGYFGPETSEAVREFQRKNGLRVDGLVGPQTWAALGYEARPTTYARQTAAA
HCCCCCCEECCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHCCCCCCCCHHHHHHHHH
VSRNDDVQLLARLVHAEARGEPYRGQVAIAAVVLNRVESPSFPNSLSGVIYQPLAFESVA
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHEECCHHHHHHC
NGQINLPPTQENLRAARAALNGWDPTYGCLFFWNPSKPVSRWIWSRRVVTTIGSHVFAQ
CCEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]