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The map label for this gene is nuoE [H]

Identifier: 220931144

GI number: 220931144

Start: 305730

End: 306140

Strand: Direct

Name: nuoE [H]

Synonym: Hore_02960

Alternate gene names: 220931144

Gene position: 305730-306140 (Clockwise)

Preceding gene: 220931143

Following gene: 220931145

Centisome position: 11.86

GC content: 38.93

Gene sequence:

>411_bases
GTGAACAGGGTTAGTTTTCTGGAAAGGTTGCATAATATACAGGAAACTTATGGCTTCATATCTGAAGGAGAGATCAGGAA
GTTGTCCCAGGAGTATAAGATGCCCAGAGCCAGGATTTATGGCGTTATTAGGTTTTATTCAATGTTTTATACAGAACCAG
TTGGTAAATATATAGTCAGGGTATGTGATAGCTTATCCTGCCACATTAATGATTCGGAAGGGATTGTAGAGGTAGTAAAA
GATTATCTCGGAATTAAAAATGGTGAGACTACTGAGGATAAGAAATTTACCCTTGAAGTAGTAGAATGCCTGGGACACTG
TGGTGAAGGCCCGGTTATGATGGTTAATGACAGAATTTATACCAGGGTTAGTCCTAATATGGCTCTTGAAATACTGAGAG
ATTGCGTATAG

Upstream 100 bases:

>100_bases
TATCTTATGGCACAAAACTTGCAATATATATTGATGAAACCTGTTTAAAGGGGGGGCAGGACTTTATTGGAGAAAAAATT
CACAAAGGAGGTTGGTGTCA

Downstream 100 bases:

>100_bases
TAAGGGGAGGGAAATAAATATGACTGAAAGACTTTTTTTAAAGCAAAATGAAGTCAGTGATTTTGATAGTTATAAGTTTC
CGGCCCTGAAAAGGGCCCTG

Product: NADH dehydrogenase I subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MNRVSFLERLHNIQETYGFISEGEIRKLSQEYKMPRARIYGVIRFYSMFYTEPVGKYIVRVCDSLSCHINDSEGIVEVVK
DYLGIKNGETTEDKKFTLEVVECLGHCGEGPVMMVNDRIYTRVSPNMALEILRDCV

Sequences:

>Translated_136_residues
MNRVSFLERLHNIQETYGFISEGEIRKLSQEYKMPRARIYGVIRFYSMFYTEPVGKYIVRVCDSLSCHINDSEGIVEVVK
DYLGIKNGETTEDKKFTLEVVECLGHCGEGPVMMVNDRIYTRVSPNMALEILRDCV
>Mature_136_residues
MNRVSFLERLHNIQETYGFISEGEIRKLSQEYKMPRARIYGVIRFYSMFYTEPVGKYIVRVCDSLSCHINDSEGIVEVVK
DYLGIKNGETTEDKKFTLEVVECLGHCGEGPVMMVNDRIYTRVSPNMALEILRDCV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=117, Percent_Identity=39.3162393162393, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788621, Length=130, Percent_Identity=36.1538461538462, Blast_Score=107, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17561328, Length=130, Percent_Identity=36.9230769230769, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859877, Length=132, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21355353, Length=130, Percent_Identity=32.3076923076923, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 15727; Mature: 15727

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVSFLERLHNIQETYGFISEGEIRKLSQEYKMPRARIYGVIRFYSMFYTEPVGKYIVR
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHH
VCDSLSCHINDSEGIVEVVKDYLGIKNGETTEDKKFTLEVVECLGHCGEGPVMMVNDRIY
HHHHCCEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEE
TRVSPNMALEILRDCV
EEECHHHHHHHHHHCC
>Mature Secondary Structure
MNRVSFLERLHNIQETYGFISEGEIRKLSQEYKMPRARIYGVIRFYSMFYTEPVGKYIVR
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHH
VCDSLSCHINDSEGIVEVVKDYLGIKNGETTEDKKFTLEVVECLGHCGEGPVMMVNDRIY
HHHHCCEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEE
TRVSPNMALEILRDCV
EEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]