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The map label for this gene is pyrAA [H]

Identifier: 220931136

GI number: 220931136

Start: 293222

End: 294316

Strand: Direct

Name: pyrAA [H]

Synonym: Hore_02880

Alternate gene names: 220931136

Gene position: 293222-294316 (Clockwise)

Preceding gene: 220931134

Following gene: 220931137

Centisome position: 11.37

GC content: 40.55

Gene sequence:

>1095_bases
ATGGAGGCTAAGTTAGTTCTGGAAGATGGTACGGTTTTTAAAGGTAAGGGATTGGGATATGAGGGGACTACATGGGGAGA
GGTTGTCTTTAATACCAGTATGACCGGGTATCAGGAAATTTTAACAGACCCGTCATACCGTGGACAGCTGGTTACGTTAA
CTTATCCCCTCATCGGTAATTATGGAATTAATCATAATGACTTTGAATCTTATGAAATTCATGCCGGGGGGTTGATTATC
AGAGAAGACTGCTCACGACCAAATCACTGGCAGTCACAAAATACCCTGGATGTTTTTCTGAAAGAGTATAAAATTACCGG
TATTACAAATATCGATACCCGTGCCCTTACCCGACGCCTCCGGTCTAAAGGTAGTATGTTTGGTGTGATTTCATCTGAAA
TTCTGGATGAAAAAGAGTTAAATAAAATAATTAAGCAGAAAATCAAAGAGAAAAGGTGCCTGGTAGAGGAAGTCAGTGTA
TCAACCCCGACCCGGATACCGGGAAAGGGTCCCAGGGTTGTGGTTATGGACTTCGGGGTTAAATATAACATTGTCAGGTA
TCTAAAAAGCCTGGGAGCTGATATTTTTGTCCTTCCTTATAGTTCATCAGCAGAAGAGGTATTAAGTTATAACCCTGACG
GGATATTACTTTCTAACGGTCCAGGGGATCCCATGGATATAAGTGAAAAAGTACCTGAAATCCAATCACTGGCTACAAAA
AATATACCCGTTTTTGGAATATGTCTGGGACATCAACTACTGGGATTAGCTTTCGGGGGCAGAACATATAAACTTAAATT
CGGGCACCATGGAGGCAATCACCCGGTCAAGGATTTAAGAACCGGTAAGGTTTTAATAACTACCCAGAACCATGGTTATG
CCCTTGAAGATGGTTTGCCGCCGGAAATTGAGGTTACCCATATAAATTTAAATGACGGTACAATAGAGGGTATAAAACAT
AAGGACTTACCGGTTTCTTCAGTACAATACCATCCTGAAGCCGGACCTGGCCCCAAAGATTCAACACTGCTATTTAACGA
ATTTATAAATAAATTAACAGGTCAGAGAAATGTAAAAAAATTGGTTAATTTTTAG

Upstream 100 bases:

>100_bases
TATTGTTAAAAATAATTGTATAATTATCTAAATAGCAAAAGGTTATATTTATGCAGGTTTATTTTATAATCATGCAAAAA
TGAAAGGGGTTGCTACCAAG

Downstream 100 bases:

>100_bases
GTAAGAACTATAAATCCTACAACTATAGGTCTAAACTATATGGTCTACAGGGTTAGGTATATAAGAAGGGGTAATGATAT
TTAAGTTTTGAATGATAGAA

Product: carbamoyl-phosphate synthase, small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEAKLVLEDGTVFKGKGLGYEGTTWGEVVFNTSMTGYQEILTDPSYRGQLVTLTYPLIGNYGINHNDFESYEIHAGGLII
REDCSRPNHWQSQNTLDVFLKEYKITGITNIDTRALTRRLRSKGSMFGVISSEILDEKELNKIIKQKIKEKRCLVEEVSV
STPTRIPGKGPRVVVMDFGVKYNIVRYLKSLGADIFVLPYSSSAEEVLSYNPDGILLSNGPGDPMDISEKVPEIQSLATK
NIPVFGICLGHQLLGLAFGGRTYKLKFGHHGGNHPVKDLRTGKVLITTQNHGYALEDGLPPEIEVTHINLNDGTIEGIKH
KDLPVSSVQYHPEAGPGPKDSTLLFNEFINKLTGQRNVKKLVNF

Sequences:

>Translated_364_residues
MEAKLVLEDGTVFKGKGLGYEGTTWGEVVFNTSMTGYQEILTDPSYRGQLVTLTYPLIGNYGINHNDFESYEIHAGGLII
REDCSRPNHWQSQNTLDVFLKEYKITGITNIDTRALTRRLRSKGSMFGVISSEILDEKELNKIIKQKIKEKRCLVEEVSV
STPTRIPGKGPRVVVMDFGVKYNIVRYLKSLGADIFVLPYSSSAEEVLSYNPDGILLSNGPGDPMDISEKVPEIQSLATK
NIPVFGICLGHQLLGLAFGGRTYKLKFGHHGGNHPVKDLRTGKVLITTQNHGYALEDGLPPEIEVTHINLNDGTIEGIKH
KDLPVSSVQYHPEAGPGPKDSTLLFNEFINKLTGQRNVKKLVNF
>Mature_364_residues
MEAKLVLEDGTVFKGKGLGYEGTTWGEVVFNTSMTGYQEILTDPSYRGQLVTLTYPLIGNYGINHNDFESYEIHAGGLII
REDCSRPNHWQSQNTLDVFLKEYKITGITNIDTRALTRRLRSKGSMFGVISSEILDEKELNKIIKQKIKEKRCLVEEVSV
STPTRIPGKGPRVVVMDFGVKYNIVRYLKSLGADIFVLPYSSSAEEVLSYNPDGILLSNGPGDPMDISEKVPEIQSLATK
NIPVFGICLGHQLLGLAFGGRTYKLKFGHHGGNHPVKDLRTGKVLITTQNHGYALEDGLPPEIEVTHINLNDGTIEGIKH
KDLPVSSVQYHPEAGPGPKDSTLLFNEFINKLTGQRNVKKLVNF

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=363, Percent_Identity=42.6997245179063, Blast_Score=291, Evalue=6e-79,
Organism=Homo sapiens, GI21361331, Length=362, Percent_Identity=36.4640883977901, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI169790915, Length=362, Percent_Identity=36.4640883977901, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=46.9816272965879, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1789760, Length=178, Percent_Identity=29.2134831460674, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=366, Percent_Identity=38.2513661202186, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=371, Percent_Identity=38.8140161725067, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=389, Percent_Identity=37.2750642673522, Blast_Score=245, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6322638, Length=161, Percent_Identity=32.9192546583851, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45555749, Length=376, Percent_Identity=41.7553191489362, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24642586, Length=376, Percent_Identity=41.7553191489362, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40386; Mature: 40386

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKLVLEDGTVFKGKGLGYEGTTWGEVVFNTSMTGYQEILTDPSYRGQLVTLTYPLIGN
CCCEEEEECCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEEEEEEEECC
YGINHNDFESYEIHAGGLIIREDCSRPNHWQSQNTLDVFLKEYKITGITNIDTRALTRRL
CCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCHHHHHEEHEEEECCCCCHHHHHHHHH
RSKGSMFGVISSEILDEKELNKIIKQKIKEKRCLVEEVSVSTPTRIPGKGPRVVVMDFGV
HHCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
KYNIVRYLKSLGADIFVLPYSSSAEEVLSYNPDGILLSNGPGDPMDISEKVPEIQSLATK
CHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHC
NIPVFGICLGHQLLGLAFGGRTYKLKFGHHGGNHPVKDLRTGKVLITTQNHGYALEDGLP
CCCEEEEEHHHHHHHEEECCEEEEEEECCCCCCCCHHHCCCCEEEEEECCCCEEECCCCC
PEIEVTHINLNDGTIEGIKHKDLPVSSVQYHPEAGPGPKDSTLLFNEFINKLTGQRNVKK
CCEEEEEEECCCCEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
LVNF
HHCC
>Mature Secondary Structure
MEAKLVLEDGTVFKGKGLGYEGTTWGEVVFNTSMTGYQEILTDPSYRGQLVTLTYPLIGN
CCCEEEEECCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEEEEEEEECC
YGINHNDFESYEIHAGGLIIREDCSRPNHWQSQNTLDVFLKEYKITGITNIDTRALTRRL
CCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCHHHHHEEHEEEECCCCCHHHHHHHHH
RSKGSMFGVISSEILDEKELNKIIKQKIKEKRCLVEEVSVSTPTRIPGKGPRVVVMDFGV
HHCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
KYNIVRYLKSLGADIFVLPYSSSAEEVLSYNPDGILLSNGPGDPMDISEKVPEIQSLATK
CHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHC
NIPVFGICLGHQLLGLAFGGRTYKLKFGHHGGNHPVKDLRTGKVLITTQNHGYALEDGLP
CCCEEEEEHHHHHHHEEECCEEEEEEECCCCCCCCHHHCCCCEEEEEECCCCEEECCCCC
PEIEVTHINLNDGTIEGIKHKDLPVSSVQYHPEAGPGPKDSTLLFNEFINKLTGQRNVKK
CCEEEEEEECCCCEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
LVNF
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8206848 [H]