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The map label for this gene is macA [H]

Identifier: 220931102

GI number: 220931102

Start: 259643

End: 261088

Strand: Direct

Name: macA [H]

Synonym: Hore_02540

Alternate gene names: 220931102

Gene position: 259643-261088 (Clockwise)

Preceding gene: 220931101

Following gene: 220931103

Centisome position: 10.07

GC content: 39.7

Gene sequence:

>1446_bases
ATGAAAAAAAAGATTATTATTCTTATATTGATAGTGGCTGTAATTGGCTCCAGTATTTATATGATGAATAAAAAAGGTGG
GAGCAAACAGGTTGCCGGGAATGTAAAACTTGTCAAAACCGTGGAGGCAAAGCCTGGTGAAATTATGACCAGGGTCCTGG
CTGATGGTAATGTTACTTTTGAAAATGACAGGGATATTAAAGTCAAGGTCTCCGGCCTTGTTGAAAGGGTCTATATCGAG
GAAGGGGATGAGGTAGCAGAAGGTGAGAAGATTATTAAAATCGATGGAGACCCTCTAAGGGATAATCTTGAACTTGCCAG
ATTAAGTTTAAAAGAGGCTTCAAAAAATTACGAAACCCTTGTTAGTAACTTTAAGAAGCAGGATAAAATAAATGAATTAA
AGCTTAAAGAGGCCAGACAGAATTTAGAACTTGCCCGCTTATCACTCAATAAAGAAAAAGAGTCACTGGAATATAAGCTT
AAGACCCTGCAGGATAAGATTGATGATGCTAAGAAGGCCCTTGATGAGGCCCGCAAAGAATGGGAAAAGCAGAAATACCT
TTTCCAGAATAAGGCTGTTGCTGAAAAGGCTGTTAAAAATGCAGAAAAAGCTTACCAGACAGCCCAGGATAACTATGAAG
ACCTCAAAGAAGAGCTCAAGTATTTGGAGCAAAAAACCATTCCGAGAACTATTGAACTGGCCAATCTGGAAGTGGGAAAA
GCTGAAAACAGTCTGGAGCTTTTAAAGAATTCTCTGGAAAAAAGTAATATTACTGAAAATGACCTGGAACTGGCCCGGAT
AAAGATTGACAGGGCAAAAAAGAATATAGCTGATATTAAGGAAGATTTGAACAGATTAGAAACCCTGGCCCCGATAACAG
GGACCATTGTCTCTCTGGAAATCAGGGAAGGCGATAAAGTTGTAGAAGGAACCACTGTCGGTAAAATTGCTGACCTTGAA
TCCTTACTTATAGAAGTTATGGTAGATGAAGTTGATGTCAATGAAGTGGCGGTAGGACAGAGTGTTACTCTAACCAGTGA
TTCTTTTGAGGATAAGCTTACAGGAGAGGTAATAAAAATAGACCCGGTTGCCACCAAGGTAGGCAATCTAAATAAATACA
GGACACAGATAGTGGTTAGTGACCCTGAAGGTGTTTTAAAACCGGGTATGTTTGTTAATGCGGAAATCGTTACATCCTAC
AAAAATAATGTAATTACCCTGCCGCCCCTGGCAGTCCTGGGAGACGAAGATAAATATGTATATGTTGTTGAGGATGGTAA
AGCTGTAAAGAGGCCGGTGGAACTCGGTCTACAAAACCTTACTAAAGTGGAAGTAAAAGGTGTTAAAGCCGGGGAACGGG
TTATCATTGGACCATTTACTGTCCTTAAAGATTTAGAACCCGGGACACCTGTGTCTGATGTTACTGAAAGAAAAAAAGAT
AAATAA

Upstream 100 bases:

>100_bases
GTAAATCTGCCTATATAACGGAACTTACCAATTACTATAAAAACCTTTATTCTATAAAGCTCTTGATAGGTGAATTTCCT
GAAGGGGAGGATATGTAGAT

Downstream 100 bases:

>100_bases
AGGGGGAGTCCAGGATGGAGGTGAAATTAAATTGTCCATAATAAAGGTATCAAATCTATCTAAAATATATGGTGAAGGTG
AGGTAGCAGTCAAGGCTCTA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MKKKIIILILIVAVIGSSIYMMNKKGGSKQVAGNVKLVKTVEAKPGEIMTRVLADGNVTFENDRDIKVKVSGLVERVYIE
EGDEVAEGEKIIKIDGDPLRDNLELARLSLKEASKNYETLVSNFKKQDKINELKLKEARQNLELARLSLNKEKESLEYKL
KTLQDKIDDAKKALDEARKEWEKQKYLFQNKAVAEKAVKNAEKAYQTAQDNYEDLKEELKYLEQKTIPRTIELANLEVGK
AENSLELLKNSLEKSNITENDLELARIKIDRAKKNIADIKEDLNRLETLAPITGTIVSLEIREGDKVVEGTTVGKIADLE
SLLIEVMVDEVDVNEVAVGQSVTLTSDSFEDKLTGEVIKIDPVATKVGNLNKYRTQIVVSDPEGVLKPGMFVNAEIVTSY
KNNVITLPPLAVLGDEDKYVYVVEDGKAVKRPVELGLQNLTKVEVKGVKAGERVIIGPFTVLKDLEPGTPVSDVTERKKD
K

Sequences:

>Translated_481_residues
MKKKIIILILIVAVIGSSIYMMNKKGGSKQVAGNVKLVKTVEAKPGEIMTRVLADGNVTFENDRDIKVKVSGLVERVYIE
EGDEVAEGEKIIKIDGDPLRDNLELARLSLKEASKNYETLVSNFKKQDKINELKLKEARQNLELARLSLNKEKESLEYKL
KTLQDKIDDAKKALDEARKEWEKQKYLFQNKAVAEKAVKNAEKAYQTAQDNYEDLKEELKYLEQKTIPRTIELANLEVGK
AENSLELLKNSLEKSNITENDLELARIKIDRAKKNIADIKEDLNRLETLAPITGTIVSLEIREGDKVVEGTTVGKIADLE
SLLIEVMVDEVDVNEVAVGQSVTLTSDSFEDKLTGEVIKIDPVATKVGNLNKYRTQIVVSDPEGVLKPGMFVNAEIVTSY
KNNVITLPPLAVLGDEDKYVYVVEDGKAVKRPVELGLQNLTKVEVKGVKAGERVIIGPFTVLKDLEPGTPVSDVTERKKD
K
>Mature_481_residues
MKKKIIILILIVAVIGSSIYMMNKKGGSKQVAGNVKLVKTVEAKPGEIMTRVLADGNVTFENDRDIKVKVSGLVERVYIE
EGDEVAEGEKIIKIDGDPLRDNLELARLSLKEASKNYETLVSNFKKQDKINELKLKEARQNLELARLSLNKEKESLEYKL
KTLQDKIDDAKKALDEARKEWEKQKYLFQNKAVAEKAVKNAEKAYQTAQDNYEDLKEELKYLEQKTIPRTIELANLEVGK
AENSLELLKNSLEKSNITENDLELARIKIDRAKKNIADIKEDLNRLETLAPITGTIVSLEIREGDKVVEGTTVGKIADLE
SLLIEVMVDEVDVNEVAVGQSVTLTSDSFEDKLTGEVIKIDPVATKVGNLNKYRTQIVVSDPEGVLKPGMFVNAEIVTSY
KNNVITLPPLAVLGDEDKYVYVVEDGKAVKRPVELGLQNLTKVEVKGVKAGERVIIGPFTVLKDLEPGTPVSDVTERKKD
K

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=204, Percent_Identity=30.3921568627451, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 53673; Mature: 53673

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIIILILIVAVIGSSIYMMNKKGGSKQVAGNVKLVKTVEAKPGEIMTRVLADGNVTF
CCCHHHHHHHHHHHHCCCEEEEECCCCCCEECCCEEEEEEECCCCHHHHHHHHCCCCEEE
ENDRDIKVKVSGLVERVYIEEGDEVAEGEKIIKIDGDPLRDNLELARLSLKEASKNYETL
CCCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHHHH
VSNFKKQDKINELKLKEARQNLELARLSLNKEKESLEYKLKTLQDKIDDAKKALDEARKE
HHHHHHHCCHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WEKQKYLFQNKAVAEKAVKNAEKAYQTAQDNYEDLKEELKYLEQKTIPRTIELANLEVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECEECCC
AENSLELLKNSLEKSNITENDLELARIKIDRAKKNIADIKEDLNRLETLAPITGTIVSLE
CCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IREGDKVVEGTTVGKIADLESLLIEVMVDEVDVNEVAVGQSVTLTSDSFEDKLTGEVIKI
ECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCCCCCEEEE
DPVATKVGNLNKYRTQIVVSDPEGVLKPGMFVNAEIVTSYKNNVITLPPLAVLGDEDKYV
CHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEHEEEEECCCCEEECCCEEEECCCCCEE
YVVEDGKAVKRPVELGLQNLTKVEVKGVKAGERVIIGPFTVLKDLEPGTPVSDVTERKKD
EEEECCHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECHHHHHCCCCCCCHHHHHHHCCC
K
C
>Mature Secondary Structure
MKKKIIILILIVAVIGSSIYMMNKKGGSKQVAGNVKLVKTVEAKPGEIMTRVLADGNVTF
CCCHHHHHHHHHHHHCCCEEEEECCCCCCEECCCEEEEEEECCCCHHHHHHHHCCCCEEE
ENDRDIKVKVSGLVERVYIEEGDEVAEGEKIIKIDGDPLRDNLELARLSLKEASKNYETL
CCCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHHHH
VSNFKKQDKINELKLKEARQNLELARLSLNKEKESLEYKLKTLQDKIDDAKKALDEARKE
HHHHHHHCCHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WEKQKYLFQNKAVAEKAVKNAEKAYQTAQDNYEDLKEELKYLEQKTIPRTIELANLEVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECEECCC
AENSLELLKNSLEKSNITENDLELARIKIDRAKKNIADIKEDLNRLETLAPITGTIVSLE
CCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IREGDKVVEGTTVGKIADLESLLIEVMVDEVDVNEVAVGQSVTLTSDSFEDKLTGEVIKI
ECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCCCCCEEEE
DPVATKVGNLNKYRTQIVVSDPEGVLKPGMFVNAEIVTSYKNNVITLPPLAVLGDEDKYV
CHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEHEEEEECCCCEEECCCEEEECCCCCEE
YVVEDGKAVKRPVELGLQNLTKVEVKGVKAGERVIIGPFTVLKDLEPGTPVSDVTERKKD
EEEECCHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECHHHHHCCCCCCCHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]