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The map label for this gene is hisF

Identifier: 220931072

GI number: 220931072

Start: 231019

End: 231777

Strand: Direct

Name: hisF

Synonym: Hore_02240

Alternate gene names: 220931072

Gene position: 231019-231777 (Clockwise)

Preceding gene: 220931071

Following gene: 220931073

Centisome position: 8.96

GC content: 43.87

Gene sequence:

>759_bases
ATGTTAACAAAAAGAATCATACCCTGTCTTGATGTTAAGGGTGGAAGGGTTGTTAAGGGGGTAAATTTTAAAGATTTAAG
GGATGAAGGTGACCCGGTGGAATTGGCCAGGTATTATGACAGAGAAGGGGCCGATGAACTGGTATTTTTAGATATTACAG
CCTCTGCTGAGAACAGGGGTATTGTCATCGATATGGTAGAAAAAACAGCTGCCAGTGTTTTTATTCCCTTTACAATAGGT
GGGGGTATCCGGACCATATCTGATATGAAAGCCATTTTAAACGCAGGGGCAGACAAGGTTTCCATAAATTCAGCAGCGGT
CAAAAATCCCGGTTTAATAGCTGAAGGAGCCAGGGTTTTTGGCAGTCAGTGTATAGTGGTAGCCATTGACTGTTGCCGGA
AAAATGGTAACTGGGAGGTTTATATTAATGGTGGTCGTACTGTCACCGGCCTTGATGCCATAAAATGGGCTCAAAAGGTT
GAGGAACTGGGGGCTGGCGAAATTTTGCTTACCAGTATGGATGCTGATGGAACTAAAGATGGTTATGATACAGAATTACT
GGCTGCTGTTTCTACAGCTGTAGAAATACCGGTTATTGCTTCAGGTGGGGCCGGAATGCCAGAACATTTACGTGAGGCTA
TTGTTGAGGGTCAGGCCGATGCTGTTCTGGCTGCCTCAATTTTTCATGAAAAAACATATTCAGTTAGTGAAGTAAAAGAG
TACCTGGCAGGTCATGGAATCCCGGTCAGGATAGAATAG

Upstream 100 bases:

>100_bases
CTATTGTAGGGAAGGCCCTTTATGAAGGCAATTTGCCTCTGGAAGTGTTAAATCAATATCCGTGAAGTGTATACATATAT
AAGAAAATGGGGGAGAAGGT

Downstream 100 bases:

>100_bases
GAATGGGGGTATTTAATGAAAGTAGATTTAGAAAACCTCAACTTTGATGACAGGGGTCTAATTCCGGCTGTTTTACAGGA
TGTAGAAAGCCGGAAGGTTT

Product: imidazole glycerol phosphate synthase subunit hisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLTKRIIPCLDVKGGRVVKGVNFKDLRDEGDPVELARYYDREGADELVFLDITASAENRGIVIDMVEKTAASVFIPFTIG
GGIRTISDMKAILNAGADKVSINSAAVKNPGLIAEGARVFGSQCIVVAIDCCRKNGNWEVYINGGRTVTGLDAIKWAQKV
EELGAGEILLTSMDADGTKDGYDTELLAAVSTAVEIPVIASGGAGMPEHLREAIVEGQADAVLAASIFHEKTYSVSEVKE
YLAGHGIPVRIE

Sequences:

>Translated_252_residues
MLTKRIIPCLDVKGGRVVKGVNFKDLRDEGDPVELARYYDREGADELVFLDITASAENRGIVIDMVEKTAASVFIPFTIG
GGIRTISDMKAILNAGADKVSINSAAVKNPGLIAEGARVFGSQCIVVAIDCCRKNGNWEVYINGGRTVTGLDAIKWAQKV
EELGAGEILLTSMDADGTKDGYDTELLAAVSTAVEIPVIASGGAGMPEHLREAIVEGQADAVLAASIFHEKTYSVSEVKE
YLAGHGIPVRIE
>Mature_252_residues
MLTKRIIPCLDVKGGRVVKGVNFKDLRDEGDPVELARYYDREGADELVFLDITASAENRGIVIDMVEKTAASVFIPFTIG
GGIRTISDMKAILNAGADKVSINSAAVKNPGLIAEGARVFGSQCIVVAIDCCRKNGNWEVYINGGRTVTGLDAIKWAQKV
EELGAGEILLTSMDADGTKDGYDTELLAAVSTAVEIPVIASGGAGMPEHLREAIVEGQADAVLAASIFHEKTYSVSEVKE
YLAGHGIPVRIE

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=47.0817120622568, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082028, Length=205, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=36.1904761904762, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_HALOH (B8D116)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507980.1
- ProteinModelPortal:   B8D116
- SMR:   B8D116
- GeneID:   7312544
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_02240
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26782; Mature: 26782

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVKGGRVVKGVNFKDLRDEGDPVELARYYDREGADELVFLDITASAENRG
CCCCCCCCEEECCCCEEEECCCHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
IVIDMVEKTAASVFIPFTIGGGIRTISDMKAILNAGADKVSINSAAVKNPGLIAEGARVF
EEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCCCEECCHHHH
GSQCIVVAIDCCRKNGNWEVYINGGRTVTGLDAIKWAQKVEELGAGEILLTSMDADGTKD
CCCEEEEEEEHHCCCCCEEEEECCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDTELLAAVSTAVEIPVIASGGAGMPEHLREAIVEGQADAVLAASIFHEKTYSVSEVKE
CCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHH
YLAGHGIPVRIE
HHHCCCCEEEEC
>Mature Secondary Structure
MLTKRIIPCLDVKGGRVVKGVNFKDLRDEGDPVELARYYDREGADELVFLDITASAENRG
CCCCCCCCEEECCCCEEEECCCHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
IVIDMVEKTAASVFIPFTIGGGIRTISDMKAILNAGADKVSINSAAVKNPGLIAEGARVF
EEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCCCEECCHHHH
GSQCIVVAIDCCRKNGNWEVYINGGRTVTGLDAIKWAQKVEELGAGEILLTSMDADGTKD
CCCEEEEEEEHHCCCCCEEEEECCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDTELLAAVSTAVEIPVIASGGAGMPEHLREAIVEGQADAVLAASIFHEKTYSVSEVKE
CCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHH
YLAGHGIPVRIE
HHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA