Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 220930943

GI number: 220930943

Start: 113314

End: 114786

Strand: Direct

Name: gltX [H]

Synonym: Hore_00950

Alternate gene names: 220930943

Gene position: 113314-114786 (Clockwise)

Preceding gene: 220930942

Following gene: 220930944

Centisome position: 4.4

GC content: 40.53

Gene sequence:

>1473_bases
ATGGGTAGTATCAGGTTAAGATTTCCTCCCAGTCCAACCGGGAGAATTCATATTGGCAATATAAGAACTGCCCTTTTTAA
CTGGTTATGGGCACGAAAACAGGGGGGCACCCTTGTTTTGAGAATTGAAGATACTGACCGGGAACGCTCGGCCACTGAAT
TTGAAGAGATTATAATCAGGGAACTTCAGTGGCTCGGGCTAGATGTTGATGAAGGAGTTAATAAAGGTGGGGATTATGGT
CCTTACCGGCAGTCTGAAAGGCTGGATATTTACAAAAAATACGCCAACAAACTCCTTGAGGAAGACAAGGCTTATTATTG
TTACTGTACCCCTGAAGAATTAGAACAGATGAGGAAAGAGGCCCGCGCCCGGGGTGAAATGCCCCGCTATAACGGCCGTT
GCCGTCATCTTACTGATGAGGAAAGAAAAAAACTGGAGTCTGAGGGGAGAAAACCCGTCATACGTTTTAAGTTACCTGAT
GAGGAGCAGGAGATTATTGTTAATGATCAGATCAAGGGTAAGGTATCATTTCAGACTTCAGTACTCGATGATTTTATTAT
ATTTAAATCTGATGGTATGCCAACCTATAATTTTGCTGTAGTTATTGATGATGCCTTAATGAAAATTACCGATGTTATCA
GGGGAGAAGACCATTTATCAAATACGCCAAAACAGCTTTTATTATACGAGGCCCTCGGGTTTGAACCACCCCGTTTTGCT
CATTTGCCCCTTATTTTAAACAAGGACAGGGAGAAATTGAAAAAACGGAGTAATGATGATGCTGTTTATATCGGGGAATA
CAGGCGTAAGGGTTATTTACCTGAAGCCCTGTTTAATTACCTGGCTTTACTGGGCTGGGCCCCCAGGGATGGTCAGGAGC
TTTTAAGCAGAGAGGAATTGATCAGACGCTTTGAATTAAAAGATGTAAATAAAAGTGGGGCTGTGTTTGATATAGATAAA
TTAAACTGGCTAAATGGTAAATATATTAGAAATGCTGACATTAAGAGAATTGTAGATCTTTCTATACCCTATCTAAAAGA
GGCTGGATTGATTACCGGAGAAGTTGATGCTAGTTTATACCGGTGGCTGGTTAAGGTTATAGATGTAGCCCGGACCGGGG
TTGATTACCTGAAACAGATACCTGAAGAAACTTCTCTCTTTTTTAAGAAAAATGTAGAGTTTGAGGATAAATCAAAAGCT
ATTGAGGAATTTAGTCAGGACGGGGTTACAGAGGTTTTCACAACACTTGTAAACAAATTAAAACAGCTTGAGACCTTGAC
CCCTGAAGCCATTGATGGTATCTTTAAAGAGATGAAAAAGGAACTCCCTGTAAGTCCCCGGACTATTTATCATCCAGTGA
GACTTGCCCTGACAGGTCTGAATTCAGGTCCTGAAATTAAAAATGTTATATATATTCTGGGGATCAATGAAGTTGAGAAA
AGGTTAAACGAGGCATTAAATATGACAGAATAG

Upstream 100 bases:

>100_bases
ATCTTAACTAATAGTAGTGTTAAATAAATAAACTTATATAGTTAAATAGATAAACTTAAAAATTTGAGTAGTGTATCCAG
TATTACAAGGGGGAATATTT

Downstream 100 bases:

>100_bases
CATTTAGCTAGCTTAAATATAATTCAGGCCTTCATTCCGGTACTAAAATACCGGAAGGAGGCTTTTATTATATCTCTAAG
CGTTGAGACATTTCTTTAGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MGSIRLRFPPSPTGRIHIGNIRTALFNWLWARKQGGTLVLRIEDTDRERSATEFEEIIIRELQWLGLDVDEGVNKGGDYG
PYRQSERLDIYKKYANKLLEEDKAYYCYCTPEELEQMRKEARARGEMPRYNGRCRHLTDEERKKLESEGRKPVIRFKLPD
EEQEIIVNDQIKGKVSFQTSVLDDFIIFKSDGMPTYNFAVVIDDALMKITDVIRGEDHLSNTPKQLLLYEALGFEPPRFA
HLPLILNKDREKLKKRSNDDAVYIGEYRRKGYLPEALFNYLALLGWAPRDGQELLSREELIRRFELKDVNKSGAVFDIDK
LNWLNGKYIRNADIKRIVDLSIPYLKEAGLITGEVDASLYRWLVKVIDVARTGVDYLKQIPEETSLFFKKNVEFEDKSKA
IEEFSQDGVTEVFTTLVNKLKQLETLTPEAIDGIFKEMKKELPVSPRTIYHPVRLALTGLNSGPEIKNVIYILGINEVEK
RLNEALNMTE

Sequences:

>Translated_490_residues
MGSIRLRFPPSPTGRIHIGNIRTALFNWLWARKQGGTLVLRIEDTDRERSATEFEEIIIRELQWLGLDVDEGVNKGGDYG
PYRQSERLDIYKKYANKLLEEDKAYYCYCTPEELEQMRKEARARGEMPRYNGRCRHLTDEERKKLESEGRKPVIRFKLPD
EEQEIIVNDQIKGKVSFQTSVLDDFIIFKSDGMPTYNFAVVIDDALMKITDVIRGEDHLSNTPKQLLLYEALGFEPPRFA
HLPLILNKDREKLKKRSNDDAVYIGEYRRKGYLPEALFNYLALLGWAPRDGQELLSREELIRRFELKDVNKSGAVFDIDK
LNWLNGKYIRNADIKRIVDLSIPYLKEAGLITGEVDASLYRWLVKVIDVARTGVDYLKQIPEETSLFFKKNVEFEDKSKA
IEEFSQDGVTEVFTTLVNKLKQLETLTPEAIDGIFKEMKKELPVSPRTIYHPVRLALTGLNSGPEIKNVIYILGINEVEK
RLNEALNMTE
>Mature_489_residues
GSIRLRFPPSPTGRIHIGNIRTALFNWLWARKQGGTLVLRIEDTDRERSATEFEEIIIRELQWLGLDVDEGVNKGGDYGP
YRQSERLDIYKKYANKLLEEDKAYYCYCTPEELEQMRKEARARGEMPRYNGRCRHLTDEERKKLESEGRKPVIRFKLPDE
EQEIIVNDQIKGKVSFQTSVLDDFIIFKSDGMPTYNFAVVIDDALMKITDVIRGEDHLSNTPKQLLLYEALGFEPPRFAH
LPLILNKDREKLKKRSNDDAVYIGEYRRKGYLPEALFNYLALLGWAPRDGQELLSREELIRRFELKDVNKSGAVFDIDKL
NWLNGKYIRNADIKRIVDLSIPYLKEAGLITGEVDASLYRWLVKVIDVARTGVDYLKQIPEETSLFFKKNVEFEDKSKAI
EEFSQDGVTEVFTTLVNKLKQLETLTPEAIDGIFKEMKKELPVSPRTIYHPVRLALTGLNSGPEIKNVIYILGINEVEKR
LNEALNMTE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=33.2, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI62241042, Length=240, Percent_Identity=27.0833333333333, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI4826960, Length=302, Percent_Identity=25.8278145695364, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788743, Length=485, Percent_Identity=38.9690721649485, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=33.4586466165414, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786895, Length=340, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=34.7022587268994, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17511007, Length=241, Percent_Identity=24.0663900414938, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=506, Percent_Identity=35.5731225296443, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324742, Length=314, Percent_Identity=26.7515923566879, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=216, Percent_Identity=28.2407407407407, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=35.2226720647773, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24649466, Length=243, Percent_Identity=28.3950617283951, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864488, Length=302, Percent_Identity=25.1655629139073, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56779; Mature: 56648

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSIRLRFPPSPTGRIHIGNIRTALFNWLWARKQGGTLVLRIEDTDRERSATEFEEIIIR
CCCEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
ELQWLGLDVDEGVNKGGDYGPYRQSERLDIYKKYANKLLEEDKAYYCYCTPEELEQMRKE
HHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
ARARGEMPRYNGRCRHLTDEERKKLESEGRKPVIRFKLPDEEQEIIVNDQIKGKVSFQTS
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCEEEEEHH
VLDDFIIFKSDGMPTYNFAVVIDDALMKITDVIRGEDHLSNTPKQLLLYEALGFEPPRFA
HHHCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCE
HLPLILNKDREKLKKRSNDDAVYIGEYRRKGYLPEALFNYLALLGWAPRDGQELLSREEL
ECCEEECCCHHHHHHCCCCCEEEEECHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IRRFELKDVNKSGAVFDIDKLNWLNGKYIRNADIKRIVDLSIPYLKEAGLITGEVDASLY
HHHHHHHCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHCCCCHHHHCCCEECCHHHHHH
RWLVKVIDVARTGVDYLKQIPEETSLFFKKNVEFEDKSKAIEEFSQDGVTEVFTTLVNKL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
KQLETLTPEAIDGIFKEMKKELPVSPRTIYHPVRLALTGLNSGPEIKNVIYILGINEVEK
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCEEEEEECHHHHH
RLNEALNMTE
HHHHHCCCCC
>Mature Secondary Structure 
GSIRLRFPPSPTGRIHIGNIRTALFNWLWARKQGGTLVLRIEDTDRERSATEFEEIIIR
CCEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
ELQWLGLDVDEGVNKGGDYGPYRQSERLDIYKKYANKLLEEDKAYYCYCTPEELEQMRKE
HHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
ARARGEMPRYNGRCRHLTDEERKKLESEGRKPVIRFKLPDEEQEIIVNDQIKGKVSFQTS
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCEEEEEHH
VLDDFIIFKSDGMPTYNFAVVIDDALMKITDVIRGEDHLSNTPKQLLLYEALGFEPPRFA
HHHCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCE
HLPLILNKDREKLKKRSNDDAVYIGEYRRKGYLPEALFNYLALLGWAPRDGQELLSREEL
ECCEEECCCHHHHHHCCCCCEEEEECHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IRRFELKDVNKSGAVFDIDKLNWLNGKYIRNADIKRIVDLSIPYLKEAGLITGEVDASLY
HHHHHHHCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHCCCCHHHHCCCEECCHHHHHH
RWLVKVIDVARTGVDYLKQIPEETSLFFKKNVEFEDKSKAIEEFSQDGVTEVFTTLVNKL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
KQLETLTPEAIDGIFKEMKKELPVSPRTIYHPVRLALTGLNSGPEIKNVIYILGINEVEK
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCEEEEEECHHHHH
RLNEALNMTE
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA