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The map label for this gene is lysS [H]

Identifier: 220930923

GI number: 220930923

Start: 86800

End: 88275

Strand: Direct

Name: lysS [H]

Synonym: Hore_00750

Alternate gene names: 220930923

Gene position: 86800-88275 (Clockwise)

Preceding gene: 220930922

Following gene: 220930924

Centisome position: 3.37

GC content: 40.51

Gene sequence:

>1476_bases
ATGACTGAAGGAAATATTGAAGATTTCAATGAATTAATTGCACAGAGGCTGGAAAATAAAAAGGAACTTGAAAAAAATGG
TATAAAAACCTATGGAGAAAAATACCCTGTTTCCAATCATGCCAGAGAAATAGAAGATAATTTTGAAGAATTTGAAGGGA
AAAAAGTATCTCTGTCCGGTAGGATTATGGCCAAGAGGACCCATGGAAAGGCTTCATTTGCTGATATTCAGGATGTGACC
GGGAAAATCCAACTCTATGTTAAAGTTGATATTGTTGGTGAAAAAGAGTATGAATTGTTTACCGGCCTGGATATAGGCGA
TATTATCGGGGTTACCGGCGAAGTATTCAAGACCAGGCGGGGACAAATTTCAGTCAAGGTACATAGTTTTCGCCTGTTGA
CTAAGTCTTTGCGTCCTTTACCTGAAAAATGGCACGGGTTAAGGGATAAGGAATTAAGGTATCGTCAGCGGTATCTTGAT
CTTATCGTAAATCCTGAAGTCCGTAAAACTTTTATTACCAGGAGTAAAATCATCCGGGAAATTAGAAAATATTTAGAAGA
TAAGGGCTTCCTTGAGGTTGAAACCCCCATGATGCATCCTATTGCCGGGGGTGCCAATGCCCGACCTTTTATTACCCACC
ACAATGCACTGGATATAGATTTATACCTGAGGATAGCACCTGAACTGTATCTGAAAAGGTTAATTGTTGGTGGTTTTGAA
AGGGTCTTTGAAATTAATAGAAATTTCCGTAATGAGGGTATGTCCTATAAACATAACCCTGAATTCACCATGCTCGAACT
TTATCAGGCCCAGGCAGACTACCATGATATGATGGATTTGACAGAAGACCTGATTTATAATGTAGCCAAAAAGGTTCTGG
GGACTACTAAAATAAGTTACCAGGGGCAGGAAATTGACCTGACTCCTCCCTGGAACCGGGTAACCATGGTTGAGGCTGTG
AAGGAGCATACCGGGGTGGATTTTAATGAAATAAAAAGTGATGAAGAAGCCCGGAAAGCTGCTGAGGGTCTTGGAATTGA
GCTTGAAGGAAAACCAAGCCGGGGTAAGGTTCTAAATGAAATTTTTGAAGAAAAGGTAGAAGATAAGCTTGTTCAGCCTA
CCTTTATTATGGATTACCCAATTGAAGTTTCACCTCTTGCTAAAAGGAAAGATGATAATCCTGACTTTACTTATAGATTT
GAGGCTTTTATTAATTCCTGGGAGATTGCCAATGCCTTTTCAGAATTAAATAATCCGATAGATCAGAGAGAAAGGTTTGA
GGAGCAGGTAAAACAGAGGGCCCAGGGTGATGATGAAGCTCAGATGATGGATGAAGATTATGTCCGGGCTCTGGAGTATG
GTATGCCTCCAACCGGAGGACTCGGTATTGGAATTGACCGGTTAATTATGGTGTTAACCGATTCTCCTTCAATCAGGGAT
GTTATCCTGTTCCCTACCATGAGACCAGAAAAATAA

Upstream 100 bases:

>100_bases
TGGTGATGTTGTAAAAGTTGAAGTACCTTCTGGTATTATGGAATATGAGATTCTTGCTATTGAAAAAACAAAAAAAACAT
GATGAATTGAGGGGGTAAAA

Downstream 100 bases:

>100_bases
ATAATTGTAAGGTTATAAGCCAGAGGGGGTAGCCCCTTTGGCTTTTTAATTAACAAATTATTTATTAATTAAAGAAAATT
ATTCAGTCAGGAGGGAATGA

Product: Lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTEGNIEDFNELIAQRLENKKELEKNGIKTYGEKYPVSNHAREIEDNFEEFEGKKVSLSGRIMAKRTHGKASFADIQDVT
GKIQLYVKVDIVGEKEYELFTGLDIGDIIGVTGEVFKTRRGQISVKVHSFRLLTKSLRPLPEKWHGLRDKELRYRQRYLD
LIVNPEVRKTFITRSKIIREIRKYLEDKGFLEVETPMMHPIAGGANARPFITHHNALDIDLYLRIAPELYLKRLIVGGFE
RVFEINRNFRNEGMSYKHNPEFTMLELYQAQADYHDMMDLTEDLIYNVAKKVLGTTKISYQGQEIDLTPPWNRVTMVEAV
KEHTGVDFNEIKSDEEARKAAEGLGIELEGKPSRGKVLNEIFEEKVEDKLVQPTFIMDYPIEVSPLAKRKDDNPDFTYRF
EAFINSWEIANAFSELNNPIDQRERFEEQVKQRAQGDDEAQMMDEDYVRALEYGMPPTGGLGIGIDRLIMVLTDSPSIRD
VILFPTMRPEK

Sequences:

>Translated_491_residues
MTEGNIEDFNELIAQRLENKKELEKNGIKTYGEKYPVSNHAREIEDNFEEFEGKKVSLSGRIMAKRTHGKASFADIQDVT
GKIQLYVKVDIVGEKEYELFTGLDIGDIIGVTGEVFKTRRGQISVKVHSFRLLTKSLRPLPEKWHGLRDKELRYRQRYLD
LIVNPEVRKTFITRSKIIREIRKYLEDKGFLEVETPMMHPIAGGANARPFITHHNALDIDLYLRIAPELYLKRLIVGGFE
RVFEINRNFRNEGMSYKHNPEFTMLELYQAQADYHDMMDLTEDLIYNVAKKVLGTTKISYQGQEIDLTPPWNRVTMVEAV
KEHTGVDFNEIKSDEEARKAAEGLGIELEGKPSRGKVLNEIFEEKVEDKLVQPTFIMDYPIEVSPLAKRKDDNPDFTYRF
EAFINSWEIANAFSELNNPIDQRERFEEQVKQRAQGDDEAQMMDEDYVRALEYGMPPTGGLGIGIDRLIMVLTDSPSIRD
VILFPTMRPEK
>Mature_490_residues
TEGNIEDFNELIAQRLENKKELEKNGIKTYGEKYPVSNHAREIEDNFEEFEGKKVSLSGRIMAKRTHGKASFADIQDVTG
KIQLYVKVDIVGEKEYELFTGLDIGDIIGVTGEVFKTRRGQISVKVHSFRLLTKSLRPLPEKWHGLRDKELRYRQRYLDL
IVNPEVRKTFITRSKIIREIRKYLEDKGFLEVETPMMHPIAGGANARPFITHHNALDIDLYLRIAPELYLKRLIVGGFER
VFEINRNFRNEGMSYKHNPEFTMLELYQAQADYHDMMDLTEDLIYNVAKKVLGTTKISYQGQEIDLTPPWNRVTMVEAVK
EHTGVDFNEIKSDEEARKAAEGLGIELEGKPSRGKVLNEIFEEKVEDKLVQPTFIMDYPIEVSPLAKRKDDNPDFTYRFE
AFINSWEIANAFSELNNPIDQRERFEEQVKQRAQGDDEAQMMDEDYVRALEYGMPPTGGLGIGIDRLIMVLTDSPSIRDV
ILFPTMRPEK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=505, Percent_Identity=43.960396039604, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI194272210, Length=505, Percent_Identity=43.960396039604, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI45439306, Length=467, Percent_Identity=23.5546038543897, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI40789249, Length=244, Percent_Identity=25.8196721311475, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=55.1307847082495, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1789256, Length=499, Percent_Identity=53.1062124248497, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=31.2883435582822, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788173, Length=254, Percent_Identity=27.9527559055118, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=452, Percent_Identity=47.1238938053097, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17535927, Length=452, Percent_Identity=47.1238938053097, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71994340, Length=452, Percent_Identity=47.1238938053097, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32566633, Length=257, Percent_Identity=25.2918287937743, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=466, Percent_Identity=46.9957081545064, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324256, Length=477, Percent_Identity=31.4465408805031, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=343, Percent_Identity=27.1137026239067, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325153, Length=256, Percent_Identity=25.78125, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640851, Length=505, Percent_Identity=44.1584158415842, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24640849, Length=505, Percent_Identity=44.1584158415842, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI17136276, Length=498, Percent_Identity=22.6907630522088, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24584738, Length=282, Percent_Identity=26.5957446808511, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56826; Mature: 56694

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGNIEDFNELIAQRLENKKELEKNGIKTYGEKYPVSNHAREIEDNFEEFEGKKVSLSG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECC
RIMAKRTHGKASFADIQDVTGKIQLYVKVDIVGEKEYELFTGLDIGDIIGVTGEVFKTRR
EEEEEECCCCCHHHHHHHCCCEEEEEEEEEEECCCCCHHEECCCHHHHHCCCHHHHHCCC
GQISVKVHSFRLLTKSLRPLPEKWHGLRDKELRYRQRYLDLIVNPEVRKTFITRSKIIRE
CEEEEEEHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IRKYLEDKGFLEVETPMMHPIAGGANARPFITHHNALDIDLYLRIAPELYLKRLIVGGFE
HHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECCEEEEEEEEEECHHHHHHHHHHHHHH
RVFEINRNFRNEGMSYKHNPEFTMLELYQAQADYHDMMDLTEDLIYNVAKKVLGTTKISY
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
QGQEIDLTPPWNRVTMVEAVKEHTGVDFNEIKSDEEARKAAEGLGIELEGKPSRGKVLNE
CCCEEECCCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCEEECCCCCCCHHHHH
IFEEKVEDKLVQPTFIMDYPIEVSPLAKRKDDNPDFTYRFEAFINSWEIANAFSELNNPI
HHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHHCCCH
DQRERFEEQVKQRAQGDDEAQMMDEDYVRALEYGMPPTGGLGIGIDRLIMVLTDSPSIRD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPTMRPEK
EEEECCCCCCC
>Mature Secondary Structure 
TEGNIEDFNELIAQRLENKKELEKNGIKTYGEKYPVSNHAREIEDNFEEFEGKKVSLSG
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECC
RIMAKRTHGKASFADIQDVTGKIQLYVKVDIVGEKEYELFTGLDIGDIIGVTGEVFKTRR
EEEEEECCCCCHHHHHHHCCCEEEEEEEEEEECCCCCHHEECCCHHHHHCCCHHHHHCCC
GQISVKVHSFRLLTKSLRPLPEKWHGLRDKELRYRQRYLDLIVNPEVRKTFITRSKIIRE
CEEEEEEHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IRKYLEDKGFLEVETPMMHPIAGGANARPFITHHNALDIDLYLRIAPELYLKRLIVGGFE
HHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECCEEEEEEEEEECHHHHHHHHHHHHHH
RVFEINRNFRNEGMSYKHNPEFTMLELYQAQADYHDMMDLTEDLIYNVAKKVLGTTKISY
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
QGQEIDLTPPWNRVTMVEAVKEHTGVDFNEIKSDEEARKAAEGLGIELEGKPSRGKVLNE
CCCEEECCCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCEEECCCCCCCHHHHH
IFEEKVEDKLVQPTFIMDYPIEVSPLAKRKDDNPDFTYRFEAFINSWEIANAFSELNNPI
HHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHHCCCH
DQRERFEEQVKQRAQGDDEAQMMDEDYVRALEYGMPPTGGLGIGIDRLIMVLTDSPSIRD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPTMRPEK
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA