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The map label for this gene is greA [H]

Identifier: 220930922

GI number: 220930922

Start: 86296

End: 86781

Strand: Direct

Name: greA [H]

Synonym: Hore_00740

Alternate gene names: 220930922

Gene position: 86296-86781 (Clockwise)

Preceding gene: 220930921

Following gene: 220930923

Centisome position: 3.35

GC content: 36.83

Gene sequence:

>486_bases
ATGGCAAACGAAGTTTTATTGACCCAGGAAGGTTATGAAAAATTGGAGAAGGAACTTGATTATCTGGTTAATGTCAAGCG
GCGTCAGGTTGCCAAACGTATTAAGGTTGCCAGGGAGTTCGGTGACATCGGTGAGAATTCCGAGTATGATGATGCCAAAA
ACGAGCAGGCCTTCATTGAAGGTAGGATCAAGGAAATAGAAAATATGCTGAGAAATGCCAGAATTATAAAAGATGAAGAA
GTAACAGGGGATACAGTTAACCTGGGAACTACTGTTAAATTAAAGGATAGATCAACCGATGAGGTTTTCTCATATACAAT
TGTTGGTTCAGCTGAAGCTGATCCTCTTAACTTTAAAATATCAAACGAATCACCTATTGGGAAAGCTATAATTGGACATA
AAATTGGTGATGTTGTAAAAGTTGAAGTACCTTCTGGTATTATGGAATATGAGATTCTTGCTATTGAAAAAACAAAAAAA
ACATGA

Upstream 100 bases:

>100_bases
TAATAATTGGAATAAATATATTAGTGTCAATGATAATTTCTGGTTATCTTGACACTAATTTTTTAGTTATCAAATTTTTA
GCAAAGGGGTTAGGGATATT

Downstream 100 bases:

>100_bases
TGAATTGAGGGGGTAAAAATGACTGAAGGAAATATTGAAGATTTCAATGAATTAATTGCACAGAGGCTGGAAAATAAAAA
GGAACTTGAAAAAAATGGTA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MANEVLLTQEGYEKLEKELDYLVNVKRRQVAKRIKVAREFGDIGENSEYDDAKNEQAFIEGRIKEIENMLRNARIIKDEE
VTGDTVNLGTTVKLKDRSTDEVFSYTIVGSAEADPLNFKISNESPIGKAIIGHKIGDVVKVEVPSGIMEYEILAIEKTKK
T

Sequences:

>Translated_161_residues
MANEVLLTQEGYEKLEKELDYLVNVKRRQVAKRIKVAREFGDIGENSEYDDAKNEQAFIEGRIKEIENMLRNARIIKDEE
VTGDTVNLGTTVKLKDRSTDEVFSYTIVGSAEADPLNFKISNESPIGKAIIGHKIGDVVKVEVPSGIMEYEILAIEKTKK
T
>Mature_160_residues
ANEVLLTQEGYEKLEKELDYLVNVKRRQVAKRIKVAREFGDIGENSEYDDAKNEQAFIEGRIKEIENMLRNARIIKDEEV
TGDTVNLGTTVKLKDRSTDEVFSYTIVGSAEADPLNFKISNESPIGKAIIGHKIGDVVKVEVPSGIMEYEILAIEKTKKT

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=41.1764705882353, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=31.1688311688312, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18135; Mature: 18004

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEVLLTQEGYEKLEKELDYLVNVKRRQVAKRIKVAREFGDIGENSEYDDAKNEQAFIE
CCCCEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
GRIKEIENMLRNARIIKDEEVTGDTVNLGTTVKLKDRSTDEVFSYTIVGSAEADPLNFKI
HHHHHHHHHHHHCEEECCCCCCCCEEECCEEEEECCCCCCCEEEEEEECCCCCCCEEEEE
SNESPIGKAIIGHKIGDVVKVEVPSGIMEYEILAIEKTKKT
CCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
ANEVLLTQEGYEKLEKELDYLVNVKRRQVAKRIKVAREFGDIGENSEYDDAKNEQAFIE
CCCEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
GRIKEIENMLRNARIIKDEEVTGDTVNLGTTVKLKDRSTDEVFSYTIVGSAEADPLNFKI
HHHHHHHHHHHHCEEECCCCCCCCEEECCEEEEECCCCCCCEEEEEEECCCCCCCEEEEE
SNESPIGKAIIGHKIGDVVKVEVPSGIMEYEILAIEKTKKT
CCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA