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The map label for this gene is tilS [H]

Identifier: 220930904

GI number: 220930904

Start: 63977

End: 65383

Strand: Direct

Name: tilS [H]

Synonym: Hore_00560

Alternate gene names: 220930904

Gene position: 63977-65383 (Clockwise)

Preceding gene: 220930903

Following gene: 220930905

Centisome position: 2.48

GC content: 36.89

Gene sequence:

>1407_bases
TTGGAGTTGCTGAAGAAATTTAGAGAATATATTAACCATCATAAACTTATTAAAAAGGGAGATGGGGTCCTTGTCGGTGT
ATCTGGAGGACCTGATTCCCTGACCCTGCTGGATATGCTGGTCAGGGTTAAAGATGAATATGGTTTGAAACTGGTTGTCT
TCCATTTAGACCATAAATTCAGGCAGGAAGCAGCCCGGGAGGCAAGGTATGTTTCAAATATAACAAACAACTATGGTCTT
AAATGTATTATTGAAGAGTTTGACGTACCCGGGTTGATGAATGATGAAGGGTTATCTCCTGAGGAAGCAGCCCGGAAAGT
CAGGTTTAATTTAATGATAAAATGGGTTAATAGTTTAAACCTGAATAAAATAGCTGTTGCCCATAATAAAGATGATCTGG
TAGAAACCGTTTTTCTCCATATGTTCCGGGGGACCGGTTTAAAAGGCTTGACCGGTATCGATCCGGTATCAAGGATTGGA
GGGGTTGAGGTAATTCACCCCCTCTTAAATATTTACAGGCAGGAAATAATGGATTATTGTCAAAGGCGAAATTTAAATCC
AGTATATGACCCTACCAATCAGGAAACAATCTATACCAGGAATAAAATAAGGCATCATATTATTCCATATATCGAAGATG
AAATCAATCCGGGATTAAAGGATGTTATTTACCAGATGGCTGAAGTAGTAAGGGAAGAAAATAACTTTCTGGATTATCAG
GCTGAAAAAAATCTTAAAAAGGTACTGGTAGATAAAGGGCAGGACTATGTTATCCTGTCACTGGAGAAACTATTTAAATT
ACCCCTGGTTATAAGGCGCAGGATTACCTCCCTTGTCAACAGCAAGCTAAAGGGAGAGGATAATAATTTATACCGGAAAC
ACTATGAGATGGTTGAGAATCTCATTAAATCAAAGGTTACCGGAAAACGTTTTGATTTGCCAGATGGGGTTGTTGTTGAA
AAAAGTTATGATAAAATAATATTCAGGAAAAATAAAAAAATAAACGAGTACTGCATAAATTTTAATATCCCAGCAGAAAT
TGACCTGCCCCGGGGAATAATATTAAAGGTGTCAGAGGAAAAACTCCCTTCAAACTGGCGGCAACTTGTTCAAAATCCAG
GTATTTGCCTTTGTGATCTTGAAAAAATAGAGTTACCATTAATTGTTAGACCGAGAAAACCTGGAGACAGATTCATTCCA
CTGGGAATGAAGGGATATAAAAAGGTAAAGAATTTTTTTATTGATGAAAAAATTCCCCGTTATCAAAGAGAGAATATTCC
TGTAATCCTGGACAACAAGGGGAGGATTATCTGGTTAGCCGGTTTAAGGATGGATGATAGATTTAAAATTACAGATGATA
CCAAAAAAATAGTCAGATTTGAAATAACCAATAATAAGGAGGAATAA

Upstream 100 bases:

>100_bases
AATTTTTAGAAAAAAAGCAGGAAATTTAAACTAATATGTAGAATAGGTTGAATAAGTTGCTATTAAAACCGGGCTTTAAA
GCCTAAAGGGGTTTGTACTC

Downstream 100 bases:

>100_bases
ACTATGCCATCAACAAATATTATGATGAAAGATATTAAAGAAATTATTATTCCTGAAGAGGTAATCCAGAATCGCATTAA
AGAACTTGGAGAGGAAATCA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MELLKKFREYINHHKLIKKGDGVLVGVSGGPDSLTLLDMLVRVKDEYGLKLVVFHLDHKFRQEAAREARYVSNITNNYGL
KCIIEEFDVPGLMNDEGLSPEEAARKVRFNLMIKWVNSLNLNKIAVAHNKDDLVETVFLHMFRGTGLKGLTGIDPVSRIG
GVEVIHPLLNIYRQEIMDYCQRRNLNPVYDPTNQETIYTRNKIRHHIIPYIEDEINPGLKDVIYQMAEVVREENNFLDYQ
AEKNLKKVLVDKGQDYVILSLEKLFKLPLVIRRRITSLVNSKLKGEDNNLYRKHYEMVENLIKSKVTGKRFDLPDGVVVE
KSYDKIIFRKNKKINEYCINFNIPAEIDLPRGIILKVSEEKLPSNWRQLVQNPGICLCDLEKIELPLIVRPRKPGDRFIP
LGMKGYKKVKNFFIDEKIPRYQRENIPVILDNKGRIIWLAGLRMDDRFKITDDTKKIVRFEITNNKEE

Sequences:

>Translated_468_residues
MELLKKFREYINHHKLIKKGDGVLVGVSGGPDSLTLLDMLVRVKDEYGLKLVVFHLDHKFRQEAAREARYVSNITNNYGL
KCIIEEFDVPGLMNDEGLSPEEAARKVRFNLMIKWVNSLNLNKIAVAHNKDDLVETVFLHMFRGTGLKGLTGIDPVSRIG
GVEVIHPLLNIYRQEIMDYCQRRNLNPVYDPTNQETIYTRNKIRHHIIPYIEDEINPGLKDVIYQMAEVVREENNFLDYQ
AEKNLKKVLVDKGQDYVILSLEKLFKLPLVIRRRITSLVNSKLKGEDNNLYRKHYEMVENLIKSKVTGKRFDLPDGVVVE
KSYDKIIFRKNKKINEYCINFNIPAEIDLPRGIILKVSEEKLPSNWRQLVQNPGICLCDLEKIELPLIVRPRKPGDRFIP
LGMKGYKKVKNFFIDEKIPRYQRENIPVILDNKGRIIWLAGLRMDDRFKITDDTKKIVRFEITNNKEE
>Mature_468_residues
MELLKKFREYINHHKLIKKGDGVLVGVSGGPDSLTLLDMLVRVKDEYGLKLVVFHLDHKFRQEAAREARYVSNITNNYGL
KCIIEEFDVPGLMNDEGLSPEEAARKVRFNLMIKWVNSLNLNKIAVAHNKDDLVETVFLHMFRGTGLKGLTGIDPVSRIG
GVEVIHPLLNIYRQEIMDYCQRRNLNPVYDPTNQETIYTRNKIRHHIIPYIEDEINPGLKDVIYQMAEVVREENNFLDYQ
AEKNLKKVLVDKGQDYVILSLEKLFKLPLVIRRRITSLVNSKLKGEDNNLYRKHYEMVENLIKSKVTGKRFDLPDGVVVE
KSYDKIIFRKNKKINEYCINFNIPAEIDLPRGIILKVSEEKLPSNWRQLVQNPGICLCDLEKIELPLIVRPRKPGDRFIP
LGMKGYKKVKNFFIDEKIPRYQRENIPVILDNKGRIIWLAGLRMDDRFKITDDTKKIVRFEITNNKEE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=214, Percent_Identity=27.5700934579439, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787606, Length=147, Percent_Identity=27.891156462585, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54613; Mature: 54613

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLKKFREYINHHKLIKKGDGVLVGVSGGPDSLTLLDMLVRVKDEYGLKLVVFHLDHKF
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHH
RQEAAREARYVSNITNNYGLKCIIEEFDVPGLMNDEGLSPEEAARKVRFNLMIKWVNSLN
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEHHCCC
LNKIAVAHNKDDLVETVFLHMFRGTGLKGLTGIDPVSRIGGVEVIHPLLNIYRQEIMDYC
CCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
QRRNLNPVYDPTNQETIYTRNKIRHHIIPYIEDEINPGLKDVIYQMAEVVREENNFLDYQ
HHCCCCCCCCCCCCCEEEEHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCH
AEKNLKKVLVDKGQDYVILSLEKLFKLPLVIRRRITSLVNSKLKGEDNNLYRKHYEMVEN
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LIKSKVTGKRFDLPDGVVVEKSYDKIIFRKNKKINEYCINFNIPAEIDLPRGIILKVSEE
HHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECHH
KLPSNWRQLVQNPGICLCDLEKIELPLIVRPRKPGDRFIPLGMKGYKKVKNFFIDEKIPR
HCCHHHHHHHCCCCEEEEEHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCC
YQRENIPVILDNKGRIIWLAGLRMDDRFKITDDTKKIVRFEITNNKEE
HHHCCCCEEECCCCCEEEEECCCCCCCEEECCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MELLKKFREYINHHKLIKKGDGVLVGVSGGPDSLTLLDMLVRVKDEYGLKLVVFHLDHKF
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHH
RQEAAREARYVSNITNNYGLKCIIEEFDVPGLMNDEGLSPEEAARKVRFNLMIKWVNSLN
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEHHCCC
LNKIAVAHNKDDLVETVFLHMFRGTGLKGLTGIDPVSRIGGVEVIHPLLNIYRQEIMDYC
CCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
QRRNLNPVYDPTNQETIYTRNKIRHHIIPYIEDEINPGLKDVIYQMAEVVREENNFLDYQ
HHCCCCCCCCCCCCCEEEEHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCH
AEKNLKKVLVDKGQDYVILSLEKLFKLPLVIRRRITSLVNSKLKGEDNNLYRKHYEMVEN
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LIKSKVTGKRFDLPDGVVVEKSYDKIIFRKNKKINEYCINFNIPAEIDLPRGIILKVSEE
HHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECHH
KLPSNWRQLVQNPGICLCDLEKIELPLIVRPRKPGDRFIPLGMKGYKKVKNFFIDEKIPR
HCCHHHHHHHCCCCEEEEEHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCC
YQRENIPVILDNKGRIIWLAGLRMDDRFKITDDTKKIVRFEITNNKEE
HHHCCCCEEECCCCCEEEEECCCCCCCEEECCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]