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The map label for this gene is kojP [H]

Identifier: 220930890

GI number: 220930890

Start: 47678

End: 50020

Strand: Direct

Name: kojP [H]

Synonym: Hore_00420

Alternate gene names: 220930890

Gene position: 47678-50020 (Clockwise)

Preceding gene: 220930889

Following gene: 220930891

Centisome position: 1.85

GC content: 39.61

Gene sequence:

>2343_bases
ATGGCGATAGCTTATAATCTAGGTAAAGGAGAATATAAAAACTGGATAATTTCTGAAACAGAATTTAATGAAAACAATCT
GCCTAAATTTGAAACTATCTTTAGTCTTGGAAATGGTTATATGGGTTTAAGGGCGGCCACAGAAGAACACTATTTTAATG
AAACAAGAGGTTGTTATGTGGCCGGAATGTTTGACAGGTTTAAAGGGGAGGTTGCCGAGCTTCCCAATATTCCTGATTAT
GTAGGGATGGAGATTAAGCTTGACGGAGAGAGATTTAACCTGAACCAGGGAAAAATTATATCTTATCACAGGTACTTAAA
CGTTAAAGACGGAGAACTGGTTAGAGAGGTGGAATGGCAAAGTCCAGCCGGAAATATTACAAAACTGGTCTTCAAACGCT
TTGTTTCTCTTGCCAATCTCCATCTGGCCGGTTTTAAGATTAAAATAATTCCAGTTAACTATTCCGGAAAGGTGGCCATT
AAAACTGGTTATAACGGGCAGGTAACCAATAGTGGTGTTCAACATTTCGTTGAAGGTGATAAAAGGGTTTTACCTGATGG
TAAATCTTATTTAACTGTCCGGACCCAGGAGTCGGGTATTTTTACAATTGTAGCCGGTAAGTTCCGTTTCCTGATAAATG
CCAGTGAAATTAATCCACAACAGCAGATCGTTACCGGTAGGCGTCAGCTTTTCCTGAGAAGCGAATATGAATTGAAAGAA
AATGAGTGCCTGGAAATGGAGAAGTGTGTAATTGTATATACCGGGCGGGATCTGGAATTTAAAGATAAGGATATTGATTC
TGGAGATATTGTTGAAACAGCTCTTACAACTCTAGATAAAGCAGGTACTAAGAGGTATGAAGAACTTTTTTCAGAACATC
GCCAGAAATGGCACAAGCTATGGCATGAGATAGATATTGAAATCGGTGGGCCTGATTTTGATCAGCTTGCCGTAAGGTTT
GCCCAGTATCATCTGGTACAGATGACCCCGTCCCATGACAGTCGTATCAGTGTTGCTGCCAAGGGGCTTTCGGGGGAAGG
GTATAAGGGTCATGTTTTCTGGGATACCGAGATTTTTATCCTTCCTTTCTTTATCTATACTTTCCCACAAATAGCCCGGA
AGTTACTCGAATATCGCTATCATACCCTGGATGGAGCTCGTAAAAAGGCCCGGGAAAATGGGTATAAAGGAGCCATGTAT
CCCTGGGAAAGTGCTGATACCGGTGAGGAAACCACCCCTGAATTTGGTGAAGTTGATATAAAGACCGGGAAACCCATCAG
GATCTGGTGTGGGGAAATAGAACAACATATTACGGCAGATGTTGCATATGCAATCTGGCATTATTATCAGGTTACCGGAG
ACAAAGAATTTATGTATAACTATGGAACTGAAATTTTTATGGAGACAGCCCGCTTCTGGGCCAGTAGACTGGAGTATAAT
CAGGGTCTGGACCGTTATGAAATTAAAGATGTCATCGGTCCTGATGAATATAGTGAACATGTTAACAATAATGCCTATAC
AAATTATATGGTGAAATGGCACCTTGAGAAGGCAATTGATATCTACAACTGGTTATCAGATGATAGCAGAGATATTCTGG
AGAAAATAATAAATAAAATTGCTTTAAAAGAAGATGAACTAAATGAATGGAAGAAAAAGAAAGATAAAATTTACCTTCCC
TTCCAGGAAGACAGTAAAGTTATTCCTCAATTTGATGGTTTTATGGACCAGGATGTCATAGATATAAGCAGCTACCGTGG
TGATGTCGGGGCTATAATGAAGGCTTATAGCTGGGATGAAATAACCAGCAGTCAGGTTATAAAACAGGCCGATGTAGTGA
TGTTACTCTATCTTCTTGGGGAAGATTTTAGCCATGAGGTTAAAGAGAAAAATTATCATTATTATGAACCTAAGACCCTT
CACGATTCTTCGTTAAGTCCCAGTATTCATGCCATTATGGGTAAGGAAATCGGTGATTTAGATGAGGCCTATAGATACTT
CAATAAATCTACCACTATTGACCTTGGCAGGAATATGAGAAGCTGTGATGCTGGTTTGCATTCAGCTTCTCTTGGAGGTA
TCTGGCAGGCAGTTGTTTTAGGCTTTGGTGGTGTTAAAGTTAAAGATAATGTTCTCAATATAGACCCCATGTTACCTGAG
AAGTGGGATTACCTGAACTTTAAGCTAAAATGGCAGGGGATGCCAATCAGGGTTGAAATTCGTAATGACAGAGTAAGTGT
CAGTTTTTTAAATGATGATAAAGCTATGCTAAAAGATGTTTCAGTAATGGTCAAGGGCCGTAACCTTCAGCTAAACAATA
ATAAAGCAGTAGTGAATCTCTAA

Upstream 100 bases:

>100_bases
TCTGATTGACTATGTTTAGAAACCTGGATCAAATTTTCAGGAATAATAATTCTGGAAACCTGGTCGGGTTAGATATAAAT
ACTTTAAAGGAGGGCTGGGA

Downstream 100 bases:

>100_bases
AACCCCCTTTAACATTAAATTATTTAATATTTGTTCCCGGGGGATATTGTTGATATAATAATTAAGGGATTTTACTGGAT
AAAATAAACAACAAGGAATG

Product: Kojibiose phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 780; Mature: 779

Protein sequence:

>780_residues
MAIAYNLGKGEYKNWIISETEFNENNLPKFETIFSLGNGYMGLRAATEEHYFNETRGCYVAGMFDRFKGEVAELPNIPDY
VGMEIKLDGERFNLNQGKIISYHRYLNVKDGELVREVEWQSPAGNITKLVFKRFVSLANLHLAGFKIKIIPVNYSGKVAI
KTGYNGQVTNSGVQHFVEGDKRVLPDGKSYLTVRTQESGIFTIVAGKFRFLINASEINPQQQIVTGRRQLFLRSEYELKE
NECLEMEKCVIVYTGRDLEFKDKDIDSGDIVETALTTLDKAGTKRYEELFSEHRQKWHKLWHEIDIEIGGPDFDQLAVRF
AQYHLVQMTPSHDSRISVAAKGLSGEGYKGHVFWDTEIFILPFFIYTFPQIARKLLEYRYHTLDGARKKARENGYKGAMY
PWESADTGEETTPEFGEVDIKTGKPIRIWCGEIEQHITADVAYAIWHYYQVTGDKEFMYNYGTEIFMETARFWASRLEYN
QGLDRYEIKDVIGPDEYSEHVNNNAYTNYMVKWHLEKAIDIYNWLSDDSRDILEKIINKIALKEDELNEWKKKKDKIYLP
FQEDSKVIPQFDGFMDQDVIDISSYRGDVGAIMKAYSWDEITSSQVIKQADVVMLLYLLGEDFSHEVKEKNYHYYEPKTL
HDSSLSPSIHAIMGKEIGDLDEAYRYFNKSTTIDLGRNMRSCDAGLHSASLGGIWQAVVLGFGGVKVKDNVLNIDPMLPE
KWDYLNFKLKWQGMPIRVEIRNDRVSVSFLNDDKAMLKDVSVMVKGRNLQLNNNKAVVNL

Sequences:

>Translated_780_residues
MAIAYNLGKGEYKNWIISETEFNENNLPKFETIFSLGNGYMGLRAATEEHYFNETRGCYVAGMFDRFKGEVAELPNIPDY
VGMEIKLDGERFNLNQGKIISYHRYLNVKDGELVREVEWQSPAGNITKLVFKRFVSLANLHLAGFKIKIIPVNYSGKVAI
KTGYNGQVTNSGVQHFVEGDKRVLPDGKSYLTVRTQESGIFTIVAGKFRFLINASEINPQQQIVTGRRQLFLRSEYELKE
NECLEMEKCVIVYTGRDLEFKDKDIDSGDIVETALTTLDKAGTKRYEELFSEHRQKWHKLWHEIDIEIGGPDFDQLAVRF
AQYHLVQMTPSHDSRISVAAKGLSGEGYKGHVFWDTEIFILPFFIYTFPQIARKLLEYRYHTLDGARKKARENGYKGAMY
PWESADTGEETTPEFGEVDIKTGKPIRIWCGEIEQHITADVAYAIWHYYQVTGDKEFMYNYGTEIFMETARFWASRLEYN
QGLDRYEIKDVIGPDEYSEHVNNNAYTNYMVKWHLEKAIDIYNWLSDDSRDILEKIINKIALKEDELNEWKKKKDKIYLP
FQEDSKVIPQFDGFMDQDVIDISSYRGDVGAIMKAYSWDEITSSQVIKQADVVMLLYLLGEDFSHEVKEKNYHYYEPKTL
HDSSLSPSIHAIMGKEIGDLDEAYRYFNKSTTIDLGRNMRSCDAGLHSASLGGIWQAVVLGFGGVKVKDNVLNIDPMLPE
KWDYLNFKLKWQGMPIRVEIRNDRVSVSFLNDDKAMLKDVSVMVKGRNLQLNNNKAVVNL
>Mature_779_residues
AIAYNLGKGEYKNWIISETEFNENNLPKFETIFSLGNGYMGLRAATEEHYFNETRGCYVAGMFDRFKGEVAELPNIPDYV
GMEIKLDGERFNLNQGKIISYHRYLNVKDGELVREVEWQSPAGNITKLVFKRFVSLANLHLAGFKIKIIPVNYSGKVAIK
TGYNGQVTNSGVQHFVEGDKRVLPDGKSYLTVRTQESGIFTIVAGKFRFLINASEINPQQQIVTGRRQLFLRSEYELKEN
ECLEMEKCVIVYTGRDLEFKDKDIDSGDIVETALTTLDKAGTKRYEELFSEHRQKWHKLWHEIDIEIGGPDFDQLAVRFA
QYHLVQMTPSHDSRISVAAKGLSGEGYKGHVFWDTEIFILPFFIYTFPQIARKLLEYRYHTLDGARKKARENGYKGAMYP
WESADTGEETTPEFGEVDIKTGKPIRIWCGEIEQHITADVAYAIWHYYQVTGDKEFMYNYGTEIFMETARFWASRLEYNQ
GLDRYEIKDVIGPDEYSEHVNNNAYTNYMVKWHLEKAIDIYNWLSDDSRDILEKIINKIALKEDELNEWKKKKDKIYLPF
QEDSKVIPQFDGFMDQDVIDISSYRGDVGAIMKAYSWDEITSSQVIKQADVVMLLYLLGEDFSHEVKEKNYHYYEPKTLH
DSSLSPSIHAIMGKEIGDLDEAYRYFNKSTTIDLGRNMRSCDAGLHSASLGGIWQAVVLGFGGVKVKDNVLNIDPMLPEK
WDYLNFKLKWQGMPIRVEIRNDRVSVSFLNDDKAMLKDVSVMVKGRNLQLNNNKAVVNL

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=447, Percent_Identity=29.5302013422819, Blast_Score=176, Evalue=6e-44,
Organism=Escherichia coli, GI1787575, Length=741, Percent_Identity=39.8110661268556, Blast_Score=557, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6325283, Length=522, Percent_Identity=24.904214559387, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24583760, Length=351, Percent_Identity=27.6353276353276, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 90032; Mature: 89901

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAYNLGKGEYKNWIISETEFNENNLPKFETIFSLGNGYMGLRAATEEHYFNETRGCYV
CEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHCCCCCCEEE
AGMFDRFKGEVAELPNIPDYVGMEIKLDGERFNLNQGKIISYHRYLNVKDGELVREVEWQ
EECHHHHCCHHHHCCCCCCCCCEEEEECCCEEECCCCCEEEEEEEEECCCCCHHHEECCC
SPAGNITKLVFKRFVSLANLHLAGFKIKIIPVNYSGKVAIKTGYNGQVTNSGVQHFVEGD
CCCCHHHHHHHHHHHHHHCEEEECEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCC
KRVLPDGKSYLTVRTQESGIFTIVAGKFRFLINASEINPQQQIVTGRRQLFLRSEYELKE
CEECCCCCCEEEEEECCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHEEEECCCCCCC
NECLEMEKCVIVYTGRDLEFKDKDIDSGDIVETALTTLDKAGTKRYEELFSEHRQKWHKL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHEIDIEIGGPDFDQLAVRFAQYHLVQMTPSHDSRISVAAKGLSGEGYKGHVFWDTEIFI
HHEEEEEECCCCHHHHHHHHHHEEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCHHHH
LPFFIYTFPQIARKLLEYRYHTLDGARKKARENGYKGAMYPWESADTGEETTPEFGEVDI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
KTGKPIRIWCGEIEQHITADVAYAIWHYYQVTGDKEFMYNYGTEIFMETARFWASRLEYN
CCCCEEEEEECHHHHHHHHHHHHHHHEEEEECCCHHEEHHCCHHHHHHHHHHHHHHHHHH
QGLDRYEIKDVIGPDEYSEHVNNNAYTNYMVKWHLEKAIDIYNWLSDDSRDILEKIINKI
CCCCCEEHHHCCCCCHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHH
ALKEDELNEWKKKKDKIYLPFQEDSKVIPQFDGFMDQDVIDISSYRGDVGAIMKAYSWDE
HCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHCCCCHHHHHHHCCCCH
ITSSQVIKQADVVMLLYLLGEDFSHEVKEKNYHYYEPKTLHDSSLSPSIHAIMGKEIGDL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHCCCCCCH
DEAYRYFNKSTTIDLGRNMRSCDAGLHSASLGGIWQAVVLGFGGVKVKDNVLNIDPMLPE
HHHHHHHCCCCEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCEEECCCCCCC
KWDYLNFKLKWQGMPIRVEIRNDRVSVSFLNDDKAMLKDVSVMVKGRNLQLNNNKAVVNL
CCCEEEEEEEECCCEEEEEEECCEEEEEEECCCHHHHHHEEEEEECCEEEECCCEEEEEC
>Mature Secondary Structure 
AIAYNLGKGEYKNWIISETEFNENNLPKFETIFSLGNGYMGLRAATEEHYFNETRGCYV
EEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHCCCCCCEEE
AGMFDRFKGEVAELPNIPDYVGMEIKLDGERFNLNQGKIISYHRYLNVKDGELVREVEWQ
EECHHHHCCHHHHCCCCCCCCCEEEEECCCEEECCCCCEEEEEEEEECCCCCHHHEECCC
SPAGNITKLVFKRFVSLANLHLAGFKIKIIPVNYSGKVAIKTGYNGQVTNSGVQHFVEGD
CCCCHHHHHHHHHHHHHHCEEEECEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCC
KRVLPDGKSYLTVRTQESGIFTIVAGKFRFLINASEINPQQQIVTGRRQLFLRSEYELKE
CEECCCCCCEEEEEECCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHEEEECCCCCCC
NECLEMEKCVIVYTGRDLEFKDKDIDSGDIVETALTTLDKAGTKRYEELFSEHRQKWHKL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHEIDIEIGGPDFDQLAVRFAQYHLVQMTPSHDSRISVAAKGLSGEGYKGHVFWDTEIFI
HHEEEEEECCCCHHHHHHHHHHEEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCHHHH
LPFFIYTFPQIARKLLEYRYHTLDGARKKARENGYKGAMYPWESADTGEETTPEFGEVDI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
KTGKPIRIWCGEIEQHITADVAYAIWHYYQVTGDKEFMYNYGTEIFMETARFWASRLEYN
CCCCEEEEEECHHHHHHHHHHHHHHHEEEEECCCHHEEHHCCHHHHHHHHHHHHHHHHHH
QGLDRYEIKDVIGPDEYSEHVNNNAYTNYMVKWHLEKAIDIYNWLSDDSRDILEKIINKI
CCCCCEEHHHCCCCCHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHH
ALKEDELNEWKKKKDKIYLPFQEDSKVIPQFDGFMDQDVIDISSYRGDVGAIMKAYSWDE
HCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHCCCCHHHHHHHCCCCH
ITSSQVIKQADVVMLLYLLGEDFSHEVKEKNYHYYEPKTLHDSSLSPSIHAIMGKEIGDL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHCCCCCCH
DEAYRYFNKSTTIDLGRNMRSCDAGLHSASLGGIWQAVVLGFGGVKVKDNVLNIDPMLPE
HHHHHHHCCCCEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCEEECCCCCCC
KWDYLNFKLKWQGMPIRVEIRNDRVSVSFLNDDKAMLKDVSVMVKGRNLQLNNNKAVVNL
CCCEEEEEEEECCCEEEEEEECCEEEEEEECCCHHHHHHEEEEEECCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA