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The map label for this gene is recF

Identifier: 220930854

GI number: 220930854

Start: 3670

End: 4797

Strand: Direct

Name: recF

Synonym: Hore_00040

Alternate gene names: 220930854

Gene position: 3670-4797 (Clockwise)

Preceding gene: 220930853

Following gene: 220930855

Centisome position: 0.14

GC content: 35.9

Gene sequence:

>1128_bases
ATGTATATTGATCGTATTTATTTGAAGGATTTTCGTAACCTGACAGAGAATTTAATAAAGTTGGATAATAGATTAAATGT
CTTTGTTGGTTTAAATGGTCAGGGAAAGACAAATTTTCTGGAAGCAGTTTACCTTATGGGGACAGCTTCTTCCCACCGTA
CTAATGCGGATAGAGAGCTGATAAGGTGGAACCAGGATCGGGCTGTTGTCCAGCTTTATCTTGTAAAAAGGGATGAAAAA
CTAAAAATATCTCTGGAAATTAATAAAAAGGTAAAAAAACTGGAGATAAATGATGTTCCCCAGGAGAGGGTTTCAGAACT
CCTGGGAAATTTGAATGTGGTTCTGTTTTCACCAGAAGATTTAAAACTGGTTAAAGAGGGTCCTCATTTCCGGAGGAAAT
TCCTTGACACAGAATTATCCCAGGTAAAACCATATTACCATTACCTGTTAAAAAAATATAATCATATTTTGAGTCAGAGA
AATAACCTTTTAAAAGAATTAATGACCGGTAATAAGTCAGATACTACCCTCCTTGAAGTCTGGGATGAGCAGCTGGTTGA
AATAGGAGCTAAAATAATACAAAACCGGATTGAGGTTATTGATAAATTAAAGATTCTGGCCAGGCTTTCCCACCGACAGA
TTACAGATGGTCTTGAGAATATAACTCTTTCTTATGAGTCCAGCCTCAGTGACAGAATTGAAGAAAAGGAATTAGAGGAA
ATAAAAATTATATTCAGGAATAAATTAGTTAATAATAGAAATGAGGAAATAACCAGGGGATATACCCTGGCAGGTCCGCA
ACGGGATGATTTAAAAATAACCATGAATGGTATCGATATCAGAAAGTACGGGTCCCAGGGCCAGCAGAGGACAGCCGCAT
TATCTTTAAAACTGGCTGAACTGGAATTTATGAAATCAGAACAGGGGGAATACCCTGTACTTTTACTTGATGATGTTTTT
TCTGAACTGGATAATAAAAGAAGGCACAGGTTAATAGATATAATGGCCCACAGGGTTCAGACTTTTATTACGGCGACAGA
TTTTTTTAATTTAAACGAAATTAATACCCCTTCGATAAAGGTATTTAAAGTTAGAAATGGCCTAATAACCAGATATAAGT
CATTATAA

Upstream 100 bases:

>100_bases
TAACGGTGAAGAAGAGTTAAGGAGAGGGCGGAAGCTGGTACCCGGAGATGAAGTACAACTGGTAGGTGAAAATACTGTAT
ACCGGGTGACCAGAGATTAA

Downstream 100 bases:

>100_bases
TAGAAAAGGCAGGTGGTCTTTCATTGTTTTTACACCTTGGTGATGGATATATGATACCCACCAGAGATATAGTACTGATA
GGTGACCTGGAAAAAGTTAA

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MYIDRIYLKDFRNLTENLIKLDNRLNVFVGLNGQGKTNFLEAVYLMGTASSHRTNADRELIRWNQDRAVVQLYLVKRDEK
LKISLEINKKVKKLEINDVPQERVSELLGNLNVVLFSPEDLKLVKEGPHFRRKFLDTELSQVKPYYHYLLKKYNHILSQR
NNLLKELMTGNKSDTTLLEVWDEQLVEIGAKIIQNRIEVIDKLKILARLSHRQITDGLENITLSYESSLSDRIEEKELEE
IKIIFRNKLVNNRNEEITRGYTLAGPQRDDLKITMNGIDIRKYGSQGQQRTAALSLKLAELEFMKSEQGEYPVLLLDDVF
SELDNKRRHRLIDIMAHRVQTFITATDFFNLNEINTPSIKVFKVRNGLITRYKSL

Sequences:

>Translated_375_residues
MYIDRIYLKDFRNLTENLIKLDNRLNVFVGLNGQGKTNFLEAVYLMGTASSHRTNADRELIRWNQDRAVVQLYLVKRDEK
LKISLEINKKVKKLEINDVPQERVSELLGNLNVVLFSPEDLKLVKEGPHFRRKFLDTELSQVKPYYHYLLKKYNHILSQR
NNLLKELMTGNKSDTTLLEVWDEQLVEIGAKIIQNRIEVIDKLKILARLSHRQITDGLENITLSYESSLSDRIEEKELEE
IKIIFRNKLVNNRNEEITRGYTLAGPQRDDLKITMNGIDIRKYGSQGQQRTAALSLKLAELEFMKSEQGEYPVLLLDDVF
SELDNKRRHRLIDIMAHRVQTFITATDFFNLNEINTPSIKVFKVRNGLITRYKSL
>Mature_375_residues
MYIDRIYLKDFRNLTENLIKLDNRLNVFVGLNGQGKTNFLEAVYLMGTASSHRTNADRELIRWNQDRAVVQLYLVKRDEK
LKISLEINKKVKKLEINDVPQERVSELLGNLNVVLFSPEDLKLVKEGPHFRRKFLDTELSQVKPYYHYLLKKYNHILSQR
NNLLKELMTGNKSDTTLLEVWDEQLVEIGAKIIQNRIEVIDKLKILARLSHRQITDGLENITLSYESSLSDRIEEKELEE
IKIIFRNKLVNNRNEEITRGYTLAGPQRDDLKITMNGIDIRKYGSQGQQRTAALSLKLAELEFMKSEQGEYPVLLLDDVF
SELDNKRRHRLIDIMAHRVQTFITATDFFNLNEINTPSIKVFKVRNGLITRYKSL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=372, Percent_Identity=29.0322580645161, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_HALOH (B8CZN7)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507762.1
- ProteinModelPortal:   B8CZN7
- SMR:   B8CZN7
- GeneID:   7314899
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_00040
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK2806137
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 43893; Mature: 43893

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIDRIYLKDFRNLTENLIKLDNRLNVFVGLNGQGKTNFLEAVYLMGTASSHRTNADREL
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEECCCCCCCCCCHHH
IRWNQDRAVVQLYLVKRDEKLKISLEINKKVKKLEINDVPQERVSELLGNLNVVLFSPED
HHCCCCCEEEEEEEEECCCEEEEEEEECCCEEEEECCCCCHHHHHHHHCCCEEEEECCCC
LKLVKEGPHFRRKFLDTELSQVKPYYHYLLKKYNHILSQRNNLLKELMTGNKSDTTLLEV
HHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
WDEQLVEIGAKIIQNRIEVIDKLKILARLSHRQITDGLENITLSYESSLSDRIEEKELEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEHHHHHHHHHHHHHHH
IKIIFRNKLVNNRNEEITRGYTLAGPQRDDLKITMNGIDIRKYGSQGQQRTAALSLKLAE
HHHHHHHHHHCCCCCHHHCCEEECCCCCCCEEEEECCEEEEHHCCCCHHHHHHHHHHHHH
LEFMKSEQGEYPVLLLDDVFSELDNKRRHRLIDIMAHRVQTFITATDFFNLNEINTPSIK
HHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
VFKVRNGLITRYKSL
EEEECCCHHHHHHCC
>Mature Secondary Structure
MYIDRIYLKDFRNLTENLIKLDNRLNVFVGLNGQGKTNFLEAVYLMGTASSHRTNADREL
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEECCCCCCCCCCHHH
IRWNQDRAVVQLYLVKRDEKLKISLEINKKVKKLEINDVPQERVSELLGNLNVVLFSPED
HHCCCCCEEEEEEEEECCCEEEEEEEECCCEEEEECCCCCHHHHHHHHCCCEEEEECCCC
LKLVKEGPHFRRKFLDTELSQVKPYYHYLLKKYNHILSQRNNLLKELMTGNKSDTTLLEV
HHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
WDEQLVEIGAKIIQNRIEVIDKLKILARLSHRQITDGLENITLSYESSLSDRIEEKELEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEHHHHHHHHHHHHHHH
IKIIFRNKLVNNRNEEITRGYTLAGPQRDDLKITMNGIDIRKYGSQGQQRTAALSLKLAE
HHHHHHHHHHCCCCCHHHCCEEECCCCCCCEEEEECCEEEEHHCCCCHHHHHHHHHHHHH
LEFMKSEQGEYPVLLLDDVFSELDNKRRHRLIDIMAHRVQTFITATDFFNLNEINTPSIK
HHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
VFKVRNGLITRYKSL
EEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA