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The map label for this gene is gidB

Identifier: 221230943

GI number: 221230943

Start: 3264032

End: 3264778

Strand: Reverse

Name: gidB

Synonym: MLBr_02708

Alternate gene names: 221230943

Gene position: 3264778-3264032 (Counterclockwise)

Preceding gene: 221230944

Following gene: 221230942

Centisome position: 99.9

GC content: 49.8

Gene sequence:

>747_bases
GTGTTTCACGTGAAACATGTCGGTTCTTACGAGGAGTTGGCATCCTTTACGTCAGTTAAAGGCAATCTTGGATTTGATAC
TGTGTTTGAAGCTGTATTCATGATTTTTGGACCACGTCTTAATATTGCTCAGCGGTATGTCGATCTACTCGCAAATACAG
GAATTGAACGTGGATTGCTTGGTCCTCATGAGGCTAACCGGCTTTGGGATCGGCATCTATTGAACAGTGCAGTGGTAGCC
GAACTTCTTGATCCTGGTGATCGGGTAGTGGATATTGGAAGTGGTGCGGGGTTGCCGGGGTTGCCGTTGGCTATTGCCCG
GCCGGATCTTCAGGTTGTGTTGCTTGAGCCACTGTTACGTCGAGTTACTTTTCTTCGAGAAGTAGTGGCTGAGCTTGGAC
TTGATGTTGAAGTGGTTCGTGGCCGAGCTGAGGAGTTGTGGGTGCGTGATCGGATCGGTGAAAGAGATGTGGCTGTATCA
CGAGCGGTAGCGGCCTTGGACAAGTTGACAAAGTGGAGCATACCGTTGTTACGACCAGGTGGTCAAATACTTGCAATCAA
AGGGGAACATGTTTTTGACGAAATCCATCAACATCGGCGTGTAATGGCCTCATTGGGCGCCGTTGATGTCATGGTGGTGG
TATGTGGTGCGAATTATTTGTGTCGTCCCGTAACAGTAGTCCTAACGCGATGCGGACAGCAGATGCGGCACAAACCAGCG
CGTGTGGGGGACAGGAAAACACAATGA

Upstream 100 bases:

>100_bases
CACGGCGGGTCATTATTTTACGTGATTAGCGGCGATTGTAGATATGGTGGAGTTGAGTGTAGCGGACGGTCACAACTGGC
AGACTTTGAACGTTTAGAGG

Downstream 100 bases:

>100_bases
GTTCTCTTCAGGGTTGGGTTGACACCCCAGCTTTGTGGTCGGCCAGTCTTACTGATCAGTCAGCTTTGTTATCGAGTGAA
ATACAAAAAACACCACCAGA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MFHVKHVGSYEELASFTSVKGNLGFDTVFEAVFMIFGPRLNIAQRYVDLLANTGIERGLLGPHEANRLWDRHLLNSAVVA
ELLDPGDRVVDIGSGAGLPGLPLAIARPDLQVVLLEPLLRRVTFLREVVAELGLDVEVVRGRAEELWVRDRIGERDVAVS
RAVAALDKLTKWSIPLLRPGGQILAIKGEHVFDEIHQHRRVMASLGAVDVMVVVCGANYLCRPVTVVLTRCGQQMRHKPA
RVGDRKTQ

Sequences:

>Translated_248_residues
MFHVKHVGSYEELASFTSVKGNLGFDTVFEAVFMIFGPRLNIAQRYVDLLANTGIERGLLGPHEANRLWDRHLLNSAVVA
ELLDPGDRVVDIGSGAGLPGLPLAIARPDLQVVLLEPLLRRVTFLREVVAELGLDVEVVRGRAEELWVRDRIGERDVAVS
RAVAALDKLTKWSIPLLRPGGQILAIKGEHVFDEIHQHRRVMASLGAVDVMVVVCGANYLCRPVTVVLTRCGQQMRHKPA
RVGDRKTQ
>Mature_248_residues
MFHVKHVGSYEELASFTSVKGNLGFDTVFEAVFMIFGPRLNIAQRYVDLLANTGIERGLLGPHEANRLWDRHLLNSAVVA
ELLDPGDRVVDIGSGAGLPGLPLAIARPDLQVVLLEPLLRRVTFLREVVAELGLDVEVVRGRAEELWVRDRIGERDVAVS
RAVAALDKLTKWSIPLLRPGGQILAIKGEHVFDEIHQHRRVMASLGAVDVMVVVCGANYLCRPVTVVLTRCGQQMRHKPA
RVGDRKTQ

Specific function: Specifically methylates the N7 position of guanosine in position 518 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=151, Percent_Identity=39.0728476821192, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_MYCLB (B8ZTN3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504359.1
- ProteinModelPortal:   B8ZTN3
- EnsemblBacteria:   EBMYCT00000085176
- GeneID:   7327352
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016057
- HOGENOM:   HBG686577
- OMA:   YELLVEW
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27427; Mature: 27427

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHVKHVGSYEELASFTSVKGNLGFDTVFEAVFMIFGPRLNIAQRYVDLLANTGIERGLL
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCC
GPHEANRLWDRHLLNSAVVAELLDPGDRVVDIGSGAGLPGLPLAIARPDLQVVLLEPLLR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHH
RVTFLREVVAELGLDVEVVRGRAEELWVRDRIGERDVAVSRAVAALDKLTKWSIPLLRPG
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCC
GQILAIKGEHVFDEIHQHRRVMASLGAVDVMVVVCGANYLCRPVTVVLTRCGQQMRHKPA
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
RVGDRKTQ
HCCCCCCC
>Mature Secondary Structure
MFHVKHVGSYEELASFTSVKGNLGFDTVFEAVFMIFGPRLNIAQRYVDLLANTGIERGLL
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCC
GPHEANRLWDRHLLNSAVVAELLDPGDRVVDIGSGAGLPGLPLAIARPDLQVVLLEPLLR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHH
RVTFLREVVAELGLDVEVVRGRAEELWVRDRIGERDVAVSRAVAALDKLTKWSIPLLRPG
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCC
GQILAIKGEHVFDEIHQHRRVMASLGAVDVMVVVCGANYLCRPVTVVLTRCGQQMRHKPA
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
RVGDRKTQ
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA