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The map label for this gene is pcnA [H]

Identifier: 221230934

GI number: 221230934

Start: 3248136

End: 3249596

Strand: Reverse

Name: pcnA [H]

Synonym: MLBr_02697

Alternate gene names: 221230934

Gene position: 3249596-3248136 (Counterclockwise)

Preceding gene: 221230940

Following gene: 221230930

Centisome position: 99.43

GC content: 60.64

Gene sequence:

>1461_bases
TTGAACGTGCCTGAAGCCGCTCAGGATACTGATCTACTGACTGTAGCTGCGGTCACCCTTAACCGGCACACCCCAGTGTT
GCGTGACCTCGGCTCGGTATTCGCTGCTTCAGGCCACGAGTTATATCTGGTGGGTGGTTCGGTCCGGGATGCGCTACTAG
GTAGATTAGATTCACCTGACCTTGATTTCGCCACTGATGCCGGTCCTGAGCAAGTGCAACAAATTATGCGGCGGTGGGCC
GACAACTTGTGGGAGACGGGTATTGAGTTCGGCACCATTGGGGTCGGCAAGGGTGACTACCGTCTGGAGATCACCACGTT
TCGCGCTGACCGTTACGACCAGGTGTCGCGCAATCCAGAGGTTCGGTTCAGTGACCGCCTCGACGACGATTTGGTGCGCC
GCGACTTCACCGTGAACGCGATGGCGGTGCGCATTGCATCTTATGGGCCTGGCGAATTTTTTGATCCGCTAGGCGGTTTG
GCAGCGTTGCGGGTCCGAGTGCTGGAGACCCCTGCTGCCCCATCGGTGTCGTTCGGCGACGACCCATTGCGGATGCTGCG
CGCCGCACGATTCGTCTCCCAACTCGGCTTTACCGTCGCCCCGCAGGTGCACGCGGCGATCGAAGAGATGGCCTCGCAGT
TGGCCCGCATCAGTGCAGAGCGAGTGTCCACCGAGCTGGACAAGCTGCTGCTTGGTAACGACCCGGCGGCCGGTATCGAC
CTGCTGGTGCAGACCGGGATGGGGGAGGTGGTGTTGCCCGAAATCGGGGACATGCGGATGGCCATCGATGAACATCATCA
ACATAAGGACGTTTACCAACATTCGCTGACCGTGTTGCGCCAGGCGATCGCGCTAGAAGATAACGGCCCGGACTTGGTGT
TGCGGTGGGCGGCGTTGCTGCATGACATAGGCAAGCCCGCCACGCGGCGCCATGAGCTGAACGGCGGCGTGAGTTTTCAC
CACCACGAGGTGGTGGGCGCCAAGATGGTCCGAAAACGAATGCGCGCGTTGAAGTACGCCAAACAACTAGTTGACGATGT
TTCGCAACTGGTGTATCTGCATCTACGGTTCCATGGCTATGGTGAAGGGAAATGGACTGATTCGGCGGTGCGCCGCTACG
TCACCGATGCCGGCCCACTGTTGTCGCGGCTGCACAAACTGGTGCGCGCTGACTGCACGACCCGCAACCAGCGCCGGGCC
GCCCGACTGCAGGACAGCTATGATCGACTGGAAGCCCGGATAGCAGAGTTGGCCGCAGAGGAGGATCTGGCCCGGGTGCG
TCCCGATCTGGACGGTAACCAGATCATGGCTCTGTTGGACATCCCGGCAGGGCCGCAAGTCGGCGAAGCGTGGCGTTTTC
TGAAGGAACTACGGCTAGACCGCGGACCACTGACCAACGAGGAGGCCACTGCGGAACTGCTGGCCTGGTGGAAAACCCGG
CAATCGCAGGGGCTCCTGTAA

Upstream 100 bases:

>100_bases
GATCACCGTTGCACCTCGTGCTCGCCTGTTGATGCAGGCTTGAAATTGCCGGGTTCCGCAACGGGTCATTCCGACCCGTA
TTACCCGCCCGACTAGGCTG

Downstream 100 bases:

>100_bases
CCCCTTCGGGGTATTGGAATAGCTGTGGGGTATTGTGCGGACCGTGGTGACGGCACAGTGCTTCCCTTGAACTGCAGGTT
GTTGACGTGTTTTGCTGTGT

Product: pcnA

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNVPEAAQDTDLLTVAAVTLNRHTPVLRDLGSVFAASGHELYLVGGSVRDALLGRLDSPDLDFATDAGPEQVQQIMRRWA
DNLWETGIEFGTIGVGKGDYRLEITTFRADRYDQVSRNPEVRFSDRLDDDLVRRDFTVNAMAVRIASYGPGEFFDPLGGL
AALRVRVLETPAAPSVSFGDDPLRMLRAARFVSQLGFTVAPQVHAAIEEMASQLARISAERVSTELDKLLLGNDPAAGID
LLVQTGMGEVVLPEIGDMRMAIDEHHQHKDVYQHSLTVLRQAIALEDNGPDLVLRWAALLHDIGKPATRRHELNGGVSFH
HHEVVGAKMVRKRMRALKYAKQLVDDVSQLVYLHLRFHGYGEGKWTDSAVRRYVTDAGPLLSRLHKLVRADCTTRNQRRA
ARLQDSYDRLEARIAELAAEEDLARVRPDLDGNQIMALLDIPAGPQVGEAWRFLKELRLDRGPLTNEEATAELLAWWKTR
QSQGLL

Sequences:

>Translated_486_residues
MNVPEAAQDTDLLTVAAVTLNRHTPVLRDLGSVFAASGHELYLVGGSVRDALLGRLDSPDLDFATDAGPEQVQQIMRRWA
DNLWETGIEFGTIGVGKGDYRLEITTFRADRYDQVSRNPEVRFSDRLDDDLVRRDFTVNAMAVRIASYGPGEFFDPLGGL
AALRVRVLETPAAPSVSFGDDPLRMLRAARFVSQLGFTVAPQVHAAIEEMASQLARISAERVSTELDKLLLGNDPAAGID
LLVQTGMGEVVLPEIGDMRMAIDEHHQHKDVYQHSLTVLRQAIALEDNGPDLVLRWAALLHDIGKPATRRHELNGGVSFH
HHEVVGAKMVRKRMRALKYAKQLVDDVSQLVYLHLRFHGYGEGKWTDSAVRRYVTDAGPLLSRLHKLVRADCTTRNQRRA
ARLQDSYDRLEARIAELAAEEDLARVRPDLDGNQIMALLDIPAGPQVGEAWRFLKELRLDRGPLTNEEATAELLAWWKTR
QSQGLL
>Mature_486_residues
MNVPEAAQDTDLLTVAAVTLNRHTPVLRDLGSVFAASGHELYLVGGSVRDALLGRLDSPDLDFATDAGPEQVQQIMRRWA
DNLWETGIEFGTIGVGKGDYRLEITTFRADRYDQVSRNPEVRFSDRLDDDLVRRDFTVNAMAVRIASYGPGEFFDPLGGL
AALRVRVLETPAAPSVSFGDDPLRMLRAARFVSQLGFTVAPQVHAAIEEMASQLARISAERVSTELDKLLLGNDPAAGID
LLVQTGMGEVVLPEIGDMRMAIDEHHQHKDVYQHSLTVLRQAIALEDNGPDLVLRWAALLHDIGKPATRRHELNGGVSFH
HHEVVGAKMVRKRMRALKYAKQLVDDVSQLVYLHLRFHGYGEGKWTDSAVRRYVTDAGPLLSRLHKLVRADCTTRNQRRA
ARLQDSYDRLEARIAELAAEEDLARVRPDLDGNQIMALLDIPAGPQVGEAWRFLKELRLDRGPLTNEEATAELLAWWKTR
QSQGLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=217, Percent_Identity=34.1013824884793, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789436, Length=342, Percent_Identity=30.9941520467836, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=31.304347826087, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71995920, Length=213, Percent_Identity=31.924882629108, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=233, Percent_Identity=31.7596566523605, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281360112, Length=220, Percent_Identity=34.5454545454545, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21357337, Length=220, Percent_Identity=34.5454545454545, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54187; Mature: 54187

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00018 EF_HAND_1 ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPEAAQDTDLLTVAAVTLNRHTPVLRDLGSVFAASGHELYLVGGSVRDALLGRLDSPD
CCCCCCCCCCCCEEHEEHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCC
LDFATDAGPEQVQQIMRRWADNLWETGIEFGTIGVGKGDYRLEITTFRADRYDQVSRNPE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEEECCCHHHHCCCCC
VRFSDRLDDDLVRRDFTVNAMAVRIASYGPGEFFDPLGGLAALRVRVLETPAAPSVSFGD
CEECCCCCHHHHHHCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DPLRMLRAARFVSQLGFTVAPQVHAAIEEMASQLARISAERVSTELDKLLLGNDPAAGID
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LLVQTGMGEVVLPEIGDMRMAIDEHHQHKDVYQHSLTVLRQAIALEDNGPDLVLRWAALL
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
HDIGKPATRRHELNGGVSFHHHEVVGAKMVRKRMRALKYAKQLVDDVSQLVYLHLRFHGY
HHHCCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GEGKWTDSAVRRYVTDAGPLLSRLHKLVRADCTTRNQRRAARLQDSYDRLEARIAELAAE
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDLARVRPDLDGNQIMALLDIPAGPQVGEAWRFLKELRLDRGPLTNEEATAELLAWWKTR
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
QSQGLL
HCCCCC
>Mature Secondary Structure
MNVPEAAQDTDLLTVAAVTLNRHTPVLRDLGSVFAASGHELYLVGGSVRDALLGRLDSPD
CCCCCCCCCCCCEEHEEHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCC
LDFATDAGPEQVQQIMRRWADNLWETGIEFGTIGVGKGDYRLEITTFRADRYDQVSRNPE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEEECCCHHHHCCCCC
VRFSDRLDDDLVRRDFTVNAMAVRIASYGPGEFFDPLGGLAALRVRVLETPAAPSVSFGD
CEECCCCCHHHHHHCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DPLRMLRAARFVSQLGFTVAPQVHAAIEEMASQLARISAERVSTELDKLLLGNDPAAGID
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LLVQTGMGEVVLPEIGDMRMAIDEHHQHKDVYQHSLTVLRQAIALEDNGPDLVLRWAALL
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
HDIGKPATRRHELNGGVSFHHHEVVGAKMVRKRMRALKYAKQLVDDVSQLVYLHLRFHGY
HHHCCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GEGKWTDSAVRRYVTDAGPLLSRLHKLVRADCTTRNQRRAARLQDSYDRLEARIAELAAE
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDLARVRPDLDGNQIMALLDIPAGPQVGEAWRFLKELRLDRGPLTNEEATAELLAWWKTR
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
QSQGLL
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA