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The map label for this gene is dnaB

Identifier: 221230923

GI number: 221230923

Start: 3230183

End: 3231997

Strand: Reverse

Name: dnaB

Synonym: MLBr_02680

Alternate gene names: 221230923

Gene position: 3231997-3230183 (Counterclockwise)

Preceding gene: 221230924

Following gene: 221230919

Centisome position: 98.9

GC content: 60.61

Gene sequence:

>1815_bases
ATGGCGGTCGTCGATGATCTGGCGCACTCAGGTATGGATGCAGTTCCACCCAGTGAAGATTTTGGCCGTCAGCCACCGCA
GGACCTAGCCGCGGAGCAGTCGGTGTTGGGCGGGATGCTGCTGAGTAAGGATGCCATTGCTGACGTTCTCGAGCGGCTGC
GTTCTGGTGACTTTTATCGTCCAGCACACCAGAATGTTTACGATGCGATCCTAGATCTGTACGGGCGAGGAGAGCCGGCC
GATGCGGTGACGGTGGCCGCCGAATTGGACCGCCGCGGATTGTTGCGCAGGATCGGTGGTGCGCCGTATCTGCACACTCT
GATCTCGACGGTGCCGACTGCCGCCAATGCAGGTTACTATGCGGGCATCGTCGCTGAAAAGGCGCTGCTGCGTCGACTGG
TGGAGGCCGGCACACGGGTGGTGCAGTACGGTTACGCCGGCGCTGAGGGTGCCGACGTGGCCGAGGTGGTCGACCGCGCG
CAGGCAGAAATCTACGACGTAGCCGAGTGCCGGCTTTCTGAGAACTACGTACCGCTCGAAGATCTACTGCAGCCGACGAT
GGATGACCTCGATGCCATCGCTTCCAATGGGGGTATCTCGCGTGGCGCGCCGACTGGCTTCACCGAACTCGATGAGGTAA
CCAACGGTTTGCATCCGGGACAGATGATCATCGTGGCGGCTCGTCCGGGTGTGGGCAAGGCGCTCGCGCTGGACACGCCG
CTGCCCACACCGACTGGTTGGACGGCGATGGGTGATGTTGCCGTCGGTGACGAGTTGCTGGCCGTTGACGAAGCACCGAC
GCGCGTGGTGGCCGCGACCGAGGTCATGCTGGGCCGGCCGTGCTACGAGATCGAGTTCTCCGACGGTACCGTCATTGTCG
CAGACGCCCAGCACCAATGGCCGACGAGTTACGGTATCCGGACGTCGGCTCAGTTGCGTTGTGGGCTGGACATTATTGCT
GCGGCCGGGTCGACACCGCGCCACGCTGGGCGACTGACCACGGCTGCATTCATGGCTCCGGTGCTGTGCATCGACTCAGT
GCGGCGGGTGCGCAGCGTTCCGGTGCGGTGTGTCGAGGTGGACAATGCTGCTCATCTGTATCTGGCTGGTCGAGGGATGG
TGCCAACGCACAACTCGACCCTTGGGCTGGACTTCATGCGGTCGTGTTCGATCAAGCATCGCCTGGCGAGCGTTATCTTC
TCTCTGGAGATGAGTAAGTCCGAGATCGTGATGCGGTTGCTTTCGGCAGAGGCGAAAATCAAGCTCGCCGATATGCGTTC
GGGTCGAATGACCGACGATGACTGGACACGGCTGGCGCGGCGGATGAGCGAAATCAGTGAGGCGCCACTGTATATCGACG
ATTCACCGAACCTGACCATGATGGAGATCCGTGCCAAGGCGCGCCGGCTGCGGCAAAAGACAAACCTGAAGCTAGTTGTG
GTCGATTACCTGCAGTTGATGACGTCAGGCAAGCGGTTCGAGTCGCGACAGGTGGAAGTCTCGGAGTTCTCCCGCCATCT
TAAATTGTTAGCCAAAGAACTTGAGCTCCCGGTAGTGGCGATCAGCCAGCTGAACCGTAGTCCCGAGCAGCGCACCGACA
AGAAGCCGATGTTGTCTGATTTGCGGGAGTCGGGAAGCCTAGAGCAGGATGCCGACGTCGTGATCTTGCTGCACCGTCCG
GATGCCTTTGATCGCGACGATCCGCGTGGTGGCGAGGCGGACTTGATTCTCGCCAAGCATCGTAACGGCCCAACCAAGAC
AGTCACTGTCGCGCATCAATTGCACTTGTCGCGATTCACCAACATGGCAAGGTAA

Upstream 100 bases:

>100_bases
GTGTTGGGGCCTTGACTTATGCTCCGAACATTGGCATATCGAATGCCCGTTCGAATGCTTATGTCAGGGTGTTTGTGTCA
GGGGGAGGTGTTTAGAGTTA

Downstream 100 bases:

>100_bases
TGGACATCAGTTGTCGAGGTGCCGCGAAGATTGACAAAAGTGCAGTGATCTGTTGGCAACCTAATTAGTCTAGATAAGAT
AAATAGTATTATTGACATAT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MAVVDDLAHSGMDAVPPSEDFGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRSGDFYRPAHQNVYDAILDLYGRGEPA
DAVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYYAGIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRA
QAEIYDVAECRLSENYVPLEDLLQPTMDDLDAIASNGGISRGAPTGFTELDEVTNGLHPGQMIIVAARPGVGKALALDTP
LPTPTGWTAMGDVAVGDELLAVDEAPTRVVAATEVMLGRPCYEIEFSDGTVIVADAQHQWPTSYGIRTSAQLRCGLDIIA
AAGSTPRHAGRLTTAAFMAPVLCIDSVRRVRSVPVRCVEVDNAAHLYLAGRGMVPTHNSTLGLDFMRSCSIKHRLASVIF
SLEMSKSEIVMRLLSAEAKIKLADMRSGRMTDDDWTRLARRMSEISEAPLYIDDSPNLTMMEIRAKARRLRQKTNLKLVV
VDYLQLMTSGKRFESRQVEVSEFSRHLKLLAKELELPVVAISQLNRSPEQRTDKKPMLSDLRESGSLEQDADVVILLHRP
DAFDRDDPRGGEADLILAKHRNGPTKTVTVAHQLHLSRFTNMAR

Sequences:

>Translated_604_residues
MAVVDDLAHSGMDAVPPSEDFGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRSGDFYRPAHQNVYDAILDLYGRGEPA
DAVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYYAGIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRA
QAEIYDVAECRLSENYVPLEDLLQPTMDDLDAIASNGGISRGAPTGFTELDEVTNGLHPGQMIIVAARPGVGKALALDTP
LPTPTGWTAMGDVAVGDELLAVDEAPTRVVAATEVMLGRPCYEIEFSDGTVIVADAQHQWPTSYGIRTSAQLRCGLDIIA
AAGSTPRHAGRLTTAAFMAPVLCIDSVRRVRSVPVRCVEVDNAAHLYLAGRGMVPTHNSTLGLDFMRSCSIKHRLASVIF
SLEMSKSEIVMRLLSAEAKIKLADMRSGRMTDDDWTRLARRMSEISEAPLYIDDSPNLTMMEIRAKARRLRQKTNLKLVV
VDYLQLMTSGKRFESRQVEVSEFSRHLKLLAKELELPVVAISQLNRSPEQRTDKKPMLSDLRESGSLEQDADVVILLHRP
DAFDRDDPRGGEADLILAKHRNGPTKTVTVAHQLHLSRFTNMAR
>Mature_603_residues
AVVDDLAHSGMDAVPPSEDFGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRSGDFYRPAHQNVYDAILDLYGRGEPAD
AVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYYAGIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRAQ
AEIYDVAECRLSENYVPLEDLLQPTMDDLDAIASNGGISRGAPTGFTELDEVTNGLHPGQMIIVAARPGVGKALALDTPL
PTPTGWTAMGDVAVGDELLAVDEAPTRVVAATEVMLGRPCYEIEFSDGTVIVADAQHQWPTSYGIRTSAQLRCGLDIIAA
AGSTPRHAGRLTTAAFMAPVLCIDSVRRVRSVPVRCVEVDNAAHLYLAGRGMVPTHNSTLGLDFMRSCSIKHRLASVIFS
LEMSKSEIVMRLLSAEAKIKLADMRSGRMTDDDWTRLARRMSEISEAPLYIDDSPNLTMMEIRAKARRLRQKTNLKLVVV
DYLQLMTSGKRFESRQVEVSEFSRHLKLLAKELELPVVAISQLNRSPEQRTDKKPMLSDLRESGSLEQDADVVILLHRPD
AFDRDDPRGGEADLILAKHRNGPTKTVTVAHQLHLSRFTNMAR

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain

Homologues:

Organism=Escherichia coli, GI1790486, Length=227, Percent_Identity=42.2907488986784, Blast_Score=188, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAB_MYCLE (P46394)

Other databases:

- EMBL:   AL022118
- EMBL:   AL022118
- EMBL:   AL583926
- EMBL:   L39923
- PIR:   F87244
- RefSeq:   NP_302709.1
- ProteinModelPortal:   P46394
- SMR:   P46394
- EnsemblBacteria:   EBMYCT00000027967
- GeneID:   910801
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2680
- NMPDR:   fig|272631.1.peg.1581
- Leproma:   ML2680
- GeneTree:   EBGT00050000016654
- HOGENOM:   HBG734566
- OMA:   KNQRTFA
- ProtClustDB:   CLSK2460635
- BioCyc:   MLEP272631:ML2680-MONOMER
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003587
- InterPro:   IPR006141
- Gene3D:   G3DSA:1.10.860.10
- SMART:   SM00306
- TIGRFAMs:   TIGR00665
- TIGRFAMs:   TIGR01443

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C; SSF48024 DNA_helicase_DnaB-like_N

EC number: =3.6.4.12

Molecular weight: Translated: 65877; Mature: 65746

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50818 INTEIN_C_TER; PS50817 INTEIN_N_TER; PS51199 SF4_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVDDLAHSGMDAVPPSEDFGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRSGDFYR
CCCHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAHQNVYDAILDLYGRGEPADAVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYY
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHH
AGIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRAQAEIYDVAECRLSENYVPLE
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLLQPTMDDLDAIASNGGISRGAPTGFTELDEVTNGLHPGQMIIVAARPGVGKALALDTP
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCC
LPTPTGWTAMGDVAVGDELLAVDEAPTRVVAATEVMLGRPCYEIEFSDGTVIVADAQHQW
CCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCC
PTSYGIRTSAQLRCGLDIIAAAGSTPRHAGRLTTAAFMAPVLCIDSVRRVRSVPVRCVEV
CCCCCCCCCCCHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DNAAHLYLAGRGMVPTHNSTLGLDFMRSCSIKHRLASVIFSLEMSKSEIVMRLLSAEAKI
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEE
KLADMRSGRMTDDDWTRLARRMSEISEAPLYIDDSPNLTMMEIRAKARRLRQKTNLKLVV
EEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEHHHHHHHHHHHHHHCCCEEEH
VDYLQLMTSGKRFESRQVEVSEFSRHLKLLAKELELPVVAISQLNRSPEQRTDKKPMLSD
HHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCCCHHHHH
LRESGSLEQDADVVILLHRPDAFDRDDPRGGEADLILAKHRNGPTKTVTVAHQLHLSRFT
HHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHH
NMAR
HCCC
>Mature Secondary Structure 
AVVDDLAHSGMDAVPPSEDFGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRSGDFYR
CCHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAHQNVYDAILDLYGRGEPADAVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYY
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHH
AGIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRAQAEIYDVAECRLSENYVPLE
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLLQPTMDDLDAIASNGGISRGAPTGFTELDEVTNGLHPGQMIIVAARPGVGKALALDTP
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCC
LPTPTGWTAMGDVAVGDELLAVDEAPTRVVAATEVMLGRPCYEIEFSDGTVIVADAQHQW
CCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCC
PTSYGIRTSAQLRCGLDIIAAAGSTPRHAGRLTTAAFMAPVLCIDSVRRVRSVPVRCVEV
CCCCCCCCCCCHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DNAAHLYLAGRGMVPTHNSTLGLDFMRSCSIKHRLASVIFSLEMSKSEIVMRLLSAEAKI
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEE
KLADMRSGRMTDDDWTRLARRMSEISEAPLYIDDSPNLTMMEIRAKARRLRQKTNLKLVV
EEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEHHHHHHHHHHHHHHCCCEEEH
VDYLQLMTSGKRFESRQVEVSEFSRHLKLLAKELELPVVAISQLNRSPEQRTDKKPMLSD
HHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCCCHHHHH
LRESGSLEQDADVVILLHRPDAFDRDDPRGGEADLILAKHRNGPTKTVTVAHQLHLSRFT
HHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHH
NMAR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002; 8969512