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The map label for this gene is umaA2 [H]

Identifier: 221230808

GI number: 221230808

Start: 2931960

End: 2932847

Strand: Direct

Name: umaA2 [H]

Synonym: MLBr_02459

Alternate gene names: 221230808

Gene position: 2931960-2932847 (Clockwise)

Preceding gene: 221230807

Following gene: 221230811

Centisome position: 89.72

GC content: 56.76

Gene sequence:

>888_bases
ATGTCCGTGCAGCTGACGCCGCATTTTAGAAACGTACAAGCCCATTACGACCTATCTGACGACTTCTTCCGCCTGTTCCT
CGACCCCACCCTGACCTACAGCTGCGCGTATTTCGAGCGTGACGACATGACGCTCGAGGAAGCGCAGCTCGCCAAGGTCG
ATCTAGCGTTGGGAAAGCTCAAGCTTGAGCCCGGGATGACGTTGCTGGACATCGGCTGTGGTTGGGGCGCCACCATGAAA
CGCGCGATTGAAATATACGACGTCAACGTTATAGGCCTGACCCTGTCCGAGAATCAGGCCGCCCACGTTCAGAAGACATT
CGACGAGTTAGACACCCCCCGAACCCGGCGGGTACTGCTGGAGGGCTGGGAGAAGTTCCACGAACCTGTCGACCGGATTG
TCTCGATCGGTGCATTCGAGCACTTCGGCCGCCAACGCTATGCACGCTTCTTCAAGATGGCCCACGAAGTCCTACCGGCC
GACAGCGTGATGTTACTGCACACCATCGTCCGTCCCACCTTTAAAGATGCCAGAGCGCGGGGCATGACTTTAACCCACGA
GATTGTTCACTTTACCCAATTCATTTTGGCTGAGATCTTCCCTGGTGGCTGGTTACCAACAGTACCGACGGTGGAAGAGC
ACGCCACCGCGGCCGGTTTCAAGTTCACACGGATCCAGTCGCTGCAGCTACACTACGCCAAAACCCTGGACTTGTGGGCC
GCCAGACTGGAAGCCAACAAGAACAAGGCGATCGCCATCCAGTCTGAAGAGGTCTACGACCGCTACATGAAGTACCTAAC
TGGGTGCGCAAAGCTGTTCCGCGACGGGTACACTGATGTCGACCAGTTCACTTTGGAAAAGCAACCAGCCACCGCACGCC
GGCATTGA

Upstream 100 bases:

>100_bases
GAACGGTTAGCATGCAGGGGATGCATCGGGGGGCCAAGCCAGGGTGTGTCTTTTTTCGCGCCATAAACGCCTCCGGGACA
CAGAAAGGCTCAATAACCGT

Downstream 100 bases:

>100_bases
GTCGCCCGCTTAACCATCAGAAAACCGTCGCGGATTTACCCCCAGAACCATCCCAAAACCACAAACTCCAGAGCCGCGCG
CAAGTCTGGAGTCATCCCCA

Product: Mycolic acid synthase

Products: NA

Alternate protein names: CMAS; Cyclopropane-fatty-acyl-phospholipid synthase; CFA synthase; Cyclopropane fatty acid synthase; Mycolic acid methyltransferase; MA-MT; S-adenosylmethionine-dependent methyltransferase; AdoMet-MT; SAM-MT [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSVQLTPHFRNVQAHYDLSDDFFRLFLDPTLTYSCAYFERDDMTLEEAQLAKVDLALGKLKLEPGMTLLDIGCGWGATMK
RAIEIYDVNVIGLTLSENQAAHVQKTFDELDTPRTRRVLLEGWEKFHEPVDRIVSIGAFEHFGRQRYARFFKMAHEVLPA
DSVMLLHTIVRPTFKDARARGMTLTHEIVHFTQFILAEIFPGGWLPTVPTVEEHATAAGFKFTRIQSLQLHYAKTLDLWA
ARLEANKNKAIAIQSEEVYDRYMKYLTGCAKLFRDGYTDVDQFTLEKQPATARRH

Sequences:

>Translated_295_residues
MSVQLTPHFRNVQAHYDLSDDFFRLFLDPTLTYSCAYFERDDMTLEEAQLAKVDLALGKLKLEPGMTLLDIGCGWGATMK
RAIEIYDVNVIGLTLSENQAAHVQKTFDELDTPRTRRVLLEGWEKFHEPVDRIVSIGAFEHFGRQRYARFFKMAHEVLPA
DSVMLLHTIVRPTFKDARARGMTLTHEIVHFTQFILAEIFPGGWLPTVPTVEEHATAAGFKFTRIQSLQLHYAKTLDLWA
ARLEANKNKAIAIQSEEVYDRYMKYLTGCAKLFRDGYTDVDQFTLEKQPATARRH
>Mature_294_residues
SVQLTPHFRNVQAHYDLSDDFFRLFLDPTLTYSCAYFERDDMTLEEAQLAKVDLALGKLKLEPGMTLLDIGCGWGATMKR
AIEIYDVNVIGLTLSENQAAHVQKTFDELDTPRTRRVLLEGWEKFHEPVDRIVSIGAFEHFGRQRYARFFKMAHEVLPAD
SVMLLHTIVRPTFKDARARGMTLTHEIVHFTQFILAEIFPGGWLPTVPTVEEHATAAGFKFTRIQSLQLHYAKTLDLWAA
RLEANKNKAIAIQSEEVYDRYMKYLTGCAKLFRDGYTDVDQFTLEKQPATARRH

Specific function: Catalyzes the conversion of a double bond to a cyclopropane ring at the proximal position of an alpha mycolic acid via the transfers of a methylene group from S-adenosyl-L- methionine. Cyclopropanated mycolic acids are key factors participating in cell en

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=262, Percent_Identity=34.7328244274809, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI133904226, Length=287, Percent_Identity=31.0104529616725, Blast_Score=128, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 33934; Mature: 33803

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQLTPHFRNVQAHYDLSDDFFRLFLDPTLTYSCAYFERDDMTLEEAQLAKVDLALGKL
CCEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHEE
KLEPGMTLLDIGCGWGATMKRAIEIYDVNVIGLTLSENQAAHVQKTFDELDTPRTRRVLL
EECCCCEEEEECCCCCHHHHHEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHH
EGWEKFHEPVDRIVSIGAFEHFGRQRYARFFKMAHEVLPADSVMLLHTIVRPTFKDARAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHC
GMTLTHEIVHFTQFILAEIFPGGWLPTVPTVEEHATAAGFKFTRIQSLQLHYAKTLDLWA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
ARLEANKNKAIAIQSEEVYDRYMKYLTGCAKLFRDGYTDVDQFTLEKQPATARRH
HHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCC
>Mature Secondary Structure 
SVQLTPHFRNVQAHYDLSDDFFRLFLDPTLTYSCAYFERDDMTLEEAQLAKVDLALGKL
CEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHEE
KLEPGMTLLDIGCGWGATMKRAIEIYDVNVIGLTLSENQAAHVQKTFDELDTPRTRRVLL
EECCCCEEEEECCCCCHHHHHEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHH
EGWEKFHEPVDRIVSIGAFEHFGRQRYARFFKMAHEVLPADSVMLLHTIVRPTFKDARAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHC
GMTLTHEIVHFTQFILAEIFPGGWLPTVPTVEEHATAAGFKFTRIQSLQLHYAKTLDLWA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
ARLEANKNKAIAIQSEEVYDRYMKYLTGCAKLFRDGYTDVDQFTLEKQPATARRH
HHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]