Click here to switch to the map view.

The map label for this gene is cmaA2

Identifier: 221230784

GI number: 221230784

Start: 2901414

End: 2902340

Strand: Direct

Name: cmaA2

Synonym: MLBr_02426

Alternate gene names: 221230784

Gene position: 2901414-2902340 (Clockwise)

Preceding gene: 221230783

Following gene: 221230793

Centisome position: 88.78

GC content: 57.93

Gene sequence:

>927_bases
ATGGTTCCGTCACAGAGCCATCCGGCCAAAACCCCTCGTAAACAGCTGAAACCACCGATCGAAGCCGTACAGTCGCATTA
CGACCGATCGAATGAGTTCTTCAAGCTGTGGCTCGATCCATCGATGACCTACAGCTGCGCATACTTTGAACGCCCCGACC
TGACGCTGGAAGAGGCCCAGCGCGCTAAGCGTGACCTCGCCTTGAGCAAGCTTGGGCTAGAACCCGGTATGACTCTGCTC
GACATCGGCTGCGGCTGGGGATCGACCATGCTGCACGCGATCGAAAAGTACGACGTTAATGTGATTGGCTTGACGCTGAG
CGCGAATCAGCTCGCCCACAACAAGCTGAAGTTTGCCGAAATAGACCACACCCGAACAGACCGGACCAAGGACGTACGGC
TGCAAGGCTGGGAACAATTCGACGAACCGGTCGACCGCATCATCTCACTCGGCGCGTTCGAGCATTTTGCTGATGGCGCA
GGCGACGCCGGATTCGAACGCTACGACAGCTTCTTCAAGATGTGCTACGACGTGCTGCCCGACGATGGCAGGATGCTGCT
GCACACAATCATCGTGCCTGATGCCAAGGAAACCAAGGAGCTGGGCTTGACGACGCCGATGAGCCTGCTCCGCTTCATCA
AGTTCATCTTGACCGAGATCTTCCCTGGCGGTCGGCTGCCGAAGATCTCGCAGGTCGACCACTATTCATCCAACGCTGGT
TTCACGGTCGAGCGGTACCATCGGATCGGGTCCCACTATGTCCCGACGCTGAACGCCTGGGCCGCAGCGCTGGAAGCTCA
CAAGGACGAGGCCATCGCACTGCAGGGGCGGCAAATCTACGACACCTACATGCACTACCTGACGGGGTGTTCAGACCTAT
TCCGCGACCGTTACACCGACGTCTGCCAGTTCACCTTGGTCAAGTAG

Upstream 100 bases:

>100_bases
GTCGTCACCAGTATTGTCACTATGCTGGGCGAATTGGCCCGTCAGGATGCTACCGCTGAAGAAACCGTCGCTGCAATCGC
TTCTATATGAAAGGCGAATC

Downstream 100 bases:

>100_bases
CTACGCGACATACAATCGCGGCACATGCTTAGCCGAAGAAGATGTTCCGGCGACCAGCGAGCAACCTATAAAGCGTCTGC
TGGATCGTCTCACGCACCTG

Product: cyclopropane mycolic acid synthase

Products: NA

Alternate protein names: CMAS; Cyclopropane-fatty-acyl-phospholipid synthase; CFA synthase; Cyclopropane fatty acid synthase; Mycolic acid methyltransferase; MA-MT; S-adenosylmethionine-dependent methyltransferase; AdoMet-MT; SAM-MT

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MVPSQSHPAKTPRKQLKPPIEAVQSHYDRSNEFFKLWLDPSMTYSCAYFERPDLTLEEAQRAKRDLALSKLGLEPGMTLL
DIGCGWGSTMLHAIEKYDVNVIGLTLSANQLAHNKLKFAEIDHTRTDRTKDVRLQGWEQFDEPVDRIISLGAFEHFADGA
GDAGFERYDSFFKMCYDVLPDDGRMLLHTIIVPDAKETKELGLTTPMSLLRFIKFILTEIFPGGRLPKISQVDHYSSNAG
FTVERYHRIGSHYVPTLNAWAAALEAHKDEAIALQGRQIYDTYMHYLTGCSDLFRDRYTDVCQFTLVK

Sequences:

>Translated_308_residues
MVPSQSHPAKTPRKQLKPPIEAVQSHYDRSNEFFKLWLDPSMTYSCAYFERPDLTLEEAQRAKRDLALSKLGLEPGMTLL
DIGCGWGSTMLHAIEKYDVNVIGLTLSANQLAHNKLKFAEIDHTRTDRTKDVRLQGWEQFDEPVDRIISLGAFEHFADGA
GDAGFERYDSFFKMCYDVLPDDGRMLLHTIIVPDAKETKELGLTTPMSLLRFIKFILTEIFPGGRLPKISQVDHYSSNAG
FTVERYHRIGSHYVPTLNAWAAALEAHKDEAIALQGRQIYDTYMHYLTGCSDLFRDRYTDVCQFTLVK
>Mature_308_residues
MVPSQSHPAKTPRKQLKPPIEAVQSHYDRSNEFFKLWLDPSMTYSCAYFERPDLTLEEAQRAKRDLALSKLGLEPGMTLL
DIGCGWGSTMLHAIEKYDVNVIGLTLSANQLAHNKLKFAEIDHTRTDRTKDVRLQGWEQFDEPVDRIISLGAFEHFADGA
GDAGFERYDSFFKMCYDVLPDDGRMLLHTIIVPDAKETKELGLTTPMSLLRFIKFILTEIFPGGRLPKISQVDHYSSNAG
FTVERYHRIGSHYVPTLNAWAAALEAHKDEAIALQGRQIYDTYMHYLTGCSDLFRDRYTDVCQFTLVK

Specific function: Catalyzes the formation of trans cyclopropanated ketomycolate or methoxymycolate through the conversion of a double bond to a cyclopropane ring at the proximal position of an oxygenated mycolic acid via the transfers of a methylene group from S-adenosyl-L

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family

Homologues:

Organism=Escherichia coli, GI1787951, Length=285, Percent_Identity=34.3859649122807, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI133904226, Length=294, Percent_Identity=29.5918367346939, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CMAS2_MYCLE (Q49807)

Other databases:

- EMBL:   U00018
- EMBL:   AL583925
- PIR:   S72886
- RefSeq:   NP_302570.1
- ProteinModelPortal:   Q49807
- SMR:   Q49807
- EnsemblBacteria:   EBMYCT00000028119
- GeneID:   908573
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2426
- NMPDR:   fig|272631.1.peg.1442
- Leproma:   ML2426
- GeneTree:   EBGT00050000016600
- HOGENOM:   HBG687502
- OMA:   QLANINI
- ProtClustDB:   CLSK790562
- BioCyc:   MLEP272631:ML2426-MONOMER
- BRENDA:   2.1.1.79
- GO:   GO:0005737
- InterPro:   IPR003333
- PIRSF:   PIRSF003085

Pfam domain/function: PF02353 CMAS

EC number: =2.1.1.79

Molecular weight: Translated: 35132; Mature: 35132

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: ACT_SITE 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPSQSHPAKTPRKQLKPPIEAVQSHYDRSNEFFKLWLDPSMTYSCAYFERPDLTLEEAQ
CCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHH
RAKRDLALSKLGLEPGMTLLDIGCGWGSTMLHAIEKYDVNVIGLTLSANQLAHNKLKFAE
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHCCHHHC
IDHTRTDRTKDVRLQGWEQFDEPVDRIISLGAFEHFADGAGDAGFERYDSFFKMCYDVLP
CCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
DDGRMLLHTIIVPDAKETKELGLTTPMSLLRFIKFILTEIFPGGRLPKISQVDHYSSNAG
CCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FTVERYHRIGSHYVPTLNAWAAALEAHKDEAIALQGRQIYDTYMHYLTGCSDLFRDRYTD
CHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VCQFTLVK
HHHHEECC
>Mature Secondary Structure
MVPSQSHPAKTPRKQLKPPIEAVQSHYDRSNEFFKLWLDPSMTYSCAYFERPDLTLEEAQ
CCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHH
RAKRDLALSKLGLEPGMTLLDIGCGWGSTMLHAIEKYDVNVIGLTLSANQLAHNKLKFAE
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHCCHHHC
IDHTRTDRTKDVRLQGWEQFDEPVDRIISLGAFEHFADGAGDAGFERYDSFFKMCYDVLP
CCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
DDGRMLLHTIIVPDAKETKELGLTTPMSLLRFIKFILTEIFPGGRLPKISQVDHYSSNAG
CCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FTVERYHRIGSHYVPTLNAWAAALEAHKDEAIALQGRQIYDTYMHYLTGCSDLFRDRYTD
CHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VCQFTLVK
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002