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The map label for this gene is lpd

Identifier: 221230758

GI number: 221230758

Start: 2856775

End: 2858178

Strand: Direct

Name: lpd

Synonym: MLBr_02387

Alternate gene names: 221230758

Gene position: 2856775-2858178 (Clockwise)

Preceding gene: 221230755

Following gene: 221230759

Centisome position: 87.41

GC content: 57.69

Gene sequence:

>1404_bases
GTGACTCACTATGATGTCGTTGTGCTCGGAGCCGGTCCGGGCGGATATGTCGCTGCCATTCGTGCTGCACAGCTCGGCTT
GAGCACAGCAGTCGTCGAACCCAAGTACTGGGGAGGGATCTGCCTCAACGTCGGCTGCATCCCGTCCAAGGTGTTATTGC
ACAACGCCGAACTCGCACACATCTTCACTAAAGAGGCGAAGACTTTCGGCATCAGCGGCGATGCCAGCTTCGACTACGGG
ATTGCCTACGACCGTAGCCGCAAGGTGTCCGAGGGTCGCGTCGCCGGCGTGCACTTCCTGATGAAGAAAAACAAGATCAC
CGAGATACACGGCTACGGCAGATTCACCGACGCCAACACGTTGTCCGTTGAATTGTCGGAAGGCGTCCCTGAAACTCCGC
TAAAGGTCACGTTCAACAACGTCATCATCGCCACCGGCAGTAAAACCCGGCTGGTGCCTGGCACGTTGCTGTCGACCAAC
GTGATTACCTACGAGGAGCAGATCCTGACCCGGGAGCTTCCCGACTCGATCGTCATCGTCGGTGCAGGGGCCATCGGTAT
AGAATTCGGCTACGTCCTGAAGAACTACGGTGTCGACGTGACGATCGTGGAATTCCTGCCGCGCGCGATGCCCAACGAGG
ACGCCGAAGTGTCTAAGGAGATCGAGAAGCAATTCAAGAAGATGGGCATCAAAATCCTTACCGGAACCAAAGTGGAGTCC
ATCTCTGACAACGGTTCGCATGTCTTGGTGGCTGTCAGCAAGGACGGACAATTCCAGGAACTCAAGGCCGACAAAGTGTT
GCAGGCCATCGGGTTTGCTCCCAACGTCGACGGTTACGGACTGGACAAGGTCGGGGTAGCTTTGACCGCCGACAAGGCGG
TCGACATCGATGACTATATGCAAACCAATGTTTCGCACATCTATGCCATCGGCGATGTTACCGGTAAGCTGCAGCTGGCC
CATGTTGCCGAAGCCCAAGGGGTGGTTGCGGCCGAGGCTATCGCCGGCGCAGAGACTTTGGCGCTCAGTGACTACCGTAT
GATGCCACGCGCAACGTTCTGCCAGCCGAATGTGGCCAGCTTCGGGCTGACCGAGCAGCAAGCCCGAGACGGGGGTTATG
ACGTCGTAGTCGCTAAGTTCCCGTTTACTGCCAACGCGAAAGCGCACGGCATGGGTGACCCCAGCGGTTTCGTCAAGCTC
GTTGCGGACGCCAAGTATGGGGAGCTGTTGGGCGGGCACATGATCGGCCATAACGTGTCCGAGTTACTACCGGAGCTCAC
GCTCGCGCAGAAATGGGATTTGACGGCCACCGAGCTGGTTCGCAACGTGCATACGCATCCGACGCTGTCAGAGGCGCTGC
AGGAGTGCTTTCACGGCCTAATCGGCCATATGATCAACTTCTGA

Upstream 100 bases:

>100_bases
GTCGTTCCTACTGACGCCCAGCGGATCTCGGTTCGCACGTTCAAGTACGCGCGTGACCCGTTCAGAGGTCACCGCGTAGC
CGGCCACTAGTGTAGCTGTC

Downstream 100 bases:

>100_bases
GCATTCAGTAACGGCTTGTGGCGGCTGGAGACAACCGATTGATTGGCAACGACACCCCCGAGATCGCAGTGGGAGCCATC
GGCGGGGTGGCCACCGGCTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of alpha keto acid dehydrogenase complexes

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTHYDVVVLGAGPGGYVAAIRAAQLGLSTAVVEPKYWGGICLNVGCIPSKVLLHNAELAHIFTKEAKTFGISGDASFDYG
IAYDRSRKVSEGRVAGVHFLMKKNKITEIHGYGRFTDANTLSVELSEGVPETPLKVTFNNVIIATGSKTRLVPGTLLSTN
VITYEEQILTRELPDSIVIVGAGAIGIEFGYVLKNYGVDVTIVEFLPRAMPNEDAEVSKEIEKQFKKMGIKILTGTKVES
ISDNGSHVLVAVSKDGQFQELKADKVLQAIGFAPNVDGYGLDKVGVALTADKAVDIDDYMQTNVSHIYAIGDVTGKLQLA
HVAEAQGVVAAEAIAGAETLALSDYRMMPRATFCQPNVASFGLTEQQARDGGYDVVVAKFPFTANAKAHGMGDPSGFVKL
VADAKYGELLGGHMIGHNVSELLPELTLAQKWDLTATELVRNVHTHPTLSEALQECFHGLIGHMINF

Sequences:

>Translated_467_residues
MTHYDVVVLGAGPGGYVAAIRAAQLGLSTAVVEPKYWGGICLNVGCIPSKVLLHNAELAHIFTKEAKTFGISGDASFDYG
IAYDRSRKVSEGRVAGVHFLMKKNKITEIHGYGRFTDANTLSVELSEGVPETPLKVTFNNVIIATGSKTRLVPGTLLSTN
VITYEEQILTRELPDSIVIVGAGAIGIEFGYVLKNYGVDVTIVEFLPRAMPNEDAEVSKEIEKQFKKMGIKILTGTKVES
ISDNGSHVLVAVSKDGQFQELKADKVLQAIGFAPNVDGYGLDKVGVALTADKAVDIDDYMQTNVSHIYAIGDVTGKLQLA
HVAEAQGVVAAEAIAGAETLALSDYRMMPRATFCQPNVASFGLTEQQARDGGYDVVVAKFPFTANAKAHGMGDPSGFVKL
VADAKYGELLGGHMIGHNVSELLPELTLAQKWDLTATELVRNVHTHPTLSEALQECFHGLIGHMINF
>Mature_466_residues
THYDVVVLGAGPGGYVAAIRAAQLGLSTAVVEPKYWGGICLNVGCIPSKVLLHNAELAHIFTKEAKTFGISGDASFDYGI
AYDRSRKVSEGRVAGVHFLMKKNKITEIHGYGRFTDANTLSVELSEGVPETPLKVTFNNVIIATGSKTRLVPGTLLSTNV
ITYEEQILTRELPDSIVIVGAGAIGIEFGYVLKNYGVDVTIVEFLPRAMPNEDAEVSKEIEKQFKKMGIKILTGTKVESI
SDNGSHVLVAVSKDGQFQELKADKVLQAIGFAPNVDGYGLDKVGVALTADKAVDIDDYMQTNVSHIYAIGDVTGKLQLAH
VAEAQGVVAAEAIAGAETLALSDYRMMPRATFCQPNVASFGLTEQQARDGGYDVVVAKFPFTANAKAHGMGDPSGFVKLV
ADAKYGELLGGHMIGHNVSELLPELTLAQKWDLTATELVRNVHTHPTLSEALQECFHGLIGHMINF

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=34.5338983050847, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=29.5744680851064, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=28.322440087146, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI148277065, Length=436, Percent_Identity=24.5412844036697, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI33519430, Length=436, Percent_Identity=24.5412844036697, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI33519428, Length=436, Percent_Identity=24.5412844036697, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI33519426, Length=436, Percent_Identity=24.5412844036697, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI148277071, Length=436, Percent_Identity=24.5412844036697, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=24.5977011494253, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI291045268, Length=431, Percent_Identity=22.737819025522, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786307, Length=472, Percent_Identity=34.9576271186441, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=27.765726681128, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789915, Length=462, Percent_Identity=29.4372294372294, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=28.6334056399132, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=38.3771929824561, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=26.3157894736842, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=439, Percent_Identity=25.9681093394077, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=439, Percent_Identity=26.1958997722096, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=26.7080745341615, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=226, Percent_Identity=29.2035398230088, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=466, Percent_Identity=37.7682403433476, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=28.3870967741935, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=27.6371308016878, Blast_Score=156, Evalue=6e-39,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=38.6411889596603, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=29.4845360824742, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=29.4845360824742, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=29.4845360824742, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=27.5154004106776, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_MYCLE (Q50068)

Other databases:

- EMBL:   U15183
- EMBL:   AL583925
- PIR:   G87207
- RefSeq:   NP_302544.1
- ProteinModelPortal:   Q50068
- SMR:   Q50068
- EnsemblBacteria:   EBMYCT00000029359
- GeneID:   908174
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2387
- NMPDR:   fig|272631.1.peg.1416
- Leproma:   ML2387
- GeneTree:   EBGT00050000014575
- HOGENOM:   HBG515043
- OMA:   PGHATTA
- ProtClustDB:   PRK07818
- BioCyc:   MLEP272631:ML2387-MONOMER
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 50078; Mature: 49947

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 446-446 BINDING 50-50 BINDING 113-113 BINDING 204-204 BINDING 312-312 BINDING 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHYDVVVLGAGPGGYVAAIRAAQLGLSTAVVEPKYWGGICLNVGCIPSKVLLHNAELAH
CCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCHHHHCCCHHHH
IFTKEAKTFGISGDASFDYGIAYDRSRKVSEGRVAGVHFLMKKNKITEIHGYGRFTDANT
HHHHCCCEEECCCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEECCCEECCCCE
LSVELSEGVPETPLKVTFNNVIIATGSKTRLVPGTLLSTNVITYEEQILTRELPDSIVIV
EEEEECCCCCCCCEEEEEEEEEEEECCCCEECCCCEEECEEEEHHHHHHHHHCCCCEEEE
GAGAIGIEFGYVLKNYGVDVTIVEFLPRAMPNEDAEVSKEIEKQFKKMGIKILTGTKVES
ECCHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCEEEC
ISDNGSHVLVAVSKDGQFQELKADKVLQAIGFAPNVDGYGLDKVGVALTADKAVDIDDYM
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHH
QTNVSHIYAIGDVTGKLQLAHVAEAQGVVAAEAIAGAETLALSDYRMMPRATFCQPNVAS
HCCCEEEEEEECCCCEEEEEEHHHCCCCEEHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
FGLTEQQARDGGYDVVVAKFPFTANAKAHGMGDPSGFVKLVADAKYGELLGGHMIGHNVS
CCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCHHHCCCHH
ELLPELTLAQKWDLTATELVRNVHTHPTLSEALQECFHGLIGHMINF
HHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THYDVVVLGAGPGGYVAAIRAAQLGLSTAVVEPKYWGGICLNVGCIPSKVLLHNAELAH
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCHHHHCCCHHHH
IFTKEAKTFGISGDASFDYGIAYDRSRKVSEGRVAGVHFLMKKNKITEIHGYGRFTDANT
HHHHCCCEEECCCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEECCCEECCCCE
LSVELSEGVPETPLKVTFNNVIIATGSKTRLVPGTLLSTNVITYEEQILTRELPDSIVIV
EEEEECCCCCCCCEEEEEEEEEEEECCCCEECCCCEEECEEEEHHHHHHHHHCCCCEEEE
GAGAIGIEFGYVLKNYGVDVTIVEFLPRAMPNEDAEVSKEIEKQFKKMGIKILTGTKVES
ECCHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCEEEC
ISDNGSHVLVAVSKDGQFQELKADKVLQAIGFAPNVDGYGLDKVGVALTADKAVDIDDYM
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHH
QTNVSHIYAIGDVTGKLQLAHVAEAQGVVAAEAIAGAETLALSDYRMMPRATFCQPNVAS
HCCCEEEEEEECCCCEEEEEEHHHCCCCEEHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
FGLTEQQARDGGYDVVVAKFPFTANAKAHGMGDPSGFVKLVADAKYGELLGGHMIGHNVS
CCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCHHHCCCHH
ELLPELTLAQKWDLTATELVRNVHTHPTLSEALQECFHGLIGHMINF
HHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002