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The map label for this gene is glpK [H]

Identifier: 221230721

GI number: 221230721

Start: 2741738

End: 2743264

Strand: Reverse

Name: glpK [H]

Synonym: MLBr_02314

Alternate gene names: 221230721

Gene position: 2743264-2741738 (Counterclockwise)

Preceding gene: 221230722

Following gene: 221230716

Centisome position: 83.94

GC content: 58.55

Gene sequence:

>1527_bases
TTGGCCGAGTTCGCTGAATTCATAGCCGCCATCGACCAGGGCACCACCAGCACCCGCTGCGTGATTTTCGATCATAATGG
TGCTGAGGTGGCCCGCCACCAGCTTGAGCACGAGCAAATCTTGCCCCGCTCCGGCTGGGTCGAACATAATCCCGTTGAGA
TTTGGGAACGCACCGCGTCAGTGCTGATGTCGGTGCTGAACGCCACTAATTTGTCAGCTAAAGATATTGCAGCGCTTGGA
ATTACTAACCAACGTGAGACTACCCTGGTGTGGAATCGGAACACCGGAAGGCCGTATTGCAATGCGATCGTTTGGCAAGA
CACCCGCACTGACCGTATCGCGGTCGCGCTTGACCGAGATGGCCGCGGAGAGGTGATCCGCCGCAAAGCCGGGTTGCCGC
CGGCGACCTATTTTTCCGGCGGGAAATTGCAATGGATCTTAGAGAACGTCGACGGGGTAGGCGCCGCTGCTGAAAACGGT
GAAGCACTCTTCGGCACCCCCGATACCTGGTTATTGTGGAATCTGACCGGTGGTCCGCGGGGTGGCGTACACGTCACCGA
TGTGACCAACGCTAGCCGGACCATGCTGATGGACTTAGAGAAGCTGGATTGGGACGACGAGCTGTTGTCGTTTTTTTCCA
TCCCTCGGGCGATGTTGCCGGCGATCGCGTCGTCTTCACCGCTGCAGCCCTACGGTGTCACTTTAGCGGACGGACCCGTC
GGTGGTGAAGTGCCGATCACAGGAGTCTTGGGCGATCAGCATGCGGCGATGGTTGGTCAGGTGTGTCTAGACGCGGGGGA
AGCGAAAAACACTTACGGCACCGGCAATTTTTTGCTGCTTAACACCGGTGAGGCTATCGTGAGATCCGGTAACGGCCTGT
TGACCACCGTTTGCTATCAGTTCGGGGATGCCAAACCCGTCTACGCGCTTGAAGGTTCGATCGCGGTGACTGGCTCAGCG
GTGCAGTGGTTACGTGATCAGTTGGGCATCATCAGCGGCGCTGCTCAAAGCGAATCGCTGGCCCGTCAGGTCGTTGACAA
TGGCGGGGTGTACTTCGTACCGGCATTTTCCGGGTTGTTTTCCCCGTATTGGCGGTCCGATGCACGAGGCGCAATTGTGG
GGCTATCACGGTTCAACACCAACGCGCATCTGGCCCGCGCGACGTTGGAAGCTATCTGTTATCAGAGTCGGGACGTGGTG
GACGCGATGGCAGCAGATTCTGGTGTGCGCCTTGATGTCTTGAAGGTCGATGGTGGTATCACCAGTAATGACTTGTGCAT
GCAGATTCAGGCCGACGTGCTGGGCGTGGACGTGGTACGCTCGGTAGTTGCTGAGACTACTGCGCTGGGCTCTGCCTATG
CGGCGGGTCTAGCGGTCGGGTTTTGGGCTGGTCCGTCAGATCTACGGGCCAATTGGCAAGAGGATAAGCGTTGGACGCCG
ACGTGGGGCGAGGACCAGCGTGCCGCGGCGTATGCGGGTTGGCGTAAAGCCGTGCAGCGGACGTTGGATTGGGTCGATGT
GTCCTGA

Upstream 100 bases:

>100_bases
GCTGTCACGTAAGGATGACAGGCTGACCTGAGCTGGAATACGCCGGGTGATAGACCATCATGGACCATGAAGTGTCACAG
TTCACGTCGAAGGAGCGCAG

Downstream 100 bases:

>100_bases
CGTAGTGCATGAGGACTGCTGACCGGGGTTCAATCTCTGTCGACGGGAAGTGGTGGCACTTTTTGTGGGTGCCCTCACTT
CCGGCTTCTCCAGTCCCGCG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAEFAEFIAAIDQGTTSTRCVIFDHNGAEVARHQLEHEQILPRSGWVEHNPVEIWERTASVLMSVLNATNLSAKDIAALG
ITNQRETTLVWNRNTGRPYCNAIVWQDTRTDRIAVALDRDGRGEVIRRKAGLPPATYFSGGKLQWILENVDGVGAAAENG
EALFGTPDTWLLWNLTGGPRGGVHVTDVTNASRTMLMDLEKLDWDDELLSFFSIPRAMLPAIASSSPLQPYGVTLADGPV
GGEVPITGVLGDQHAAMVGQVCLDAGEAKNTYGTGNFLLLNTGEAIVRSGNGLLTTVCYQFGDAKPVYALEGSIAVTGSA
VQWLRDQLGIISGAAQSESLARQVVDNGGVYFVPAFSGLFSPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVV
DAMAADSGVRLDVLKVDGGITSNDLCMQIQADVLGVDVVRSVVAETTALGSAYAAGLAVGFWAGPSDLRANWQEDKRWTP
TWGEDQRAAAYAGWRKAVQRTLDWVDVS

Sequences:

>Translated_508_residues
MAEFAEFIAAIDQGTTSTRCVIFDHNGAEVARHQLEHEQILPRSGWVEHNPVEIWERTASVLMSVLNATNLSAKDIAALG
ITNQRETTLVWNRNTGRPYCNAIVWQDTRTDRIAVALDRDGRGEVIRRKAGLPPATYFSGGKLQWILENVDGVGAAAENG
EALFGTPDTWLLWNLTGGPRGGVHVTDVTNASRTMLMDLEKLDWDDELLSFFSIPRAMLPAIASSSPLQPYGVTLADGPV
GGEVPITGVLGDQHAAMVGQVCLDAGEAKNTYGTGNFLLLNTGEAIVRSGNGLLTTVCYQFGDAKPVYALEGSIAVTGSA
VQWLRDQLGIISGAAQSESLARQVVDNGGVYFVPAFSGLFSPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVV
DAMAADSGVRLDVLKVDGGITSNDLCMQIQADVLGVDVVRSVVAETTALGSAYAAGLAVGFWAGPSDLRANWQEDKRWTP
TWGEDQRAAAYAGWRKAVQRTLDWVDVS
>Mature_507_residues
AEFAEFIAAIDQGTTSTRCVIFDHNGAEVARHQLEHEQILPRSGWVEHNPVEIWERTASVLMSVLNATNLSAKDIAALGI
TNQRETTLVWNRNTGRPYCNAIVWQDTRTDRIAVALDRDGRGEVIRRKAGLPPATYFSGGKLQWILENVDGVGAAAENGE
ALFGTPDTWLLWNLTGGPRGGVHVTDVTNASRTMLMDLEKLDWDDELLSFFSIPRAMLPAIASSSPLQPYGVTLADGPVG
GEVPITGVLGDQHAAMVGQVCLDAGEAKNTYGTGNFLLLNTGEAIVRSGNGLLTTVCYQFGDAKPVYALEGSIAVTGSAV
QWLRDQLGIISGAAQSESLARQVVDNGGVYFVPAFSGLFSPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVVD
AMAADSGVRLDVLKVDGGITSNDLCMQIQADVLGVDVVRSVVAETTALGSAYAAGLAVGFWAGPSDLRANWQEDKRWTPT
WGEDQRAAAYAGWRKAVQRTLDWVDVS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=514, Percent_Identity=48.0544747081712, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI189409120, Length=514, Percent_Identity=48.0544747081712, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI42794763, Length=519, Percent_Identity=47.5915221579961, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI41393575, Length=509, Percent_Identity=46.5618860510805, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI88196792, Length=516, Percent_Identity=30.8139534883721, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1790361, Length=500, Percent_Identity=51.4, Blast_Score=510, Evalue=1e-145,
Organism=Escherichia coli, GI1789987, Length=503, Percent_Identity=24.4532803180915, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1790005, Length=497, Percent_Identity=23.1388329979879, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=508, Percent_Identity=44.0944881889764, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71986140, Length=528, Percent_Identity=24.0530303030303, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=26.578073089701, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=487, Percent_Identity=42.5051334702259, Blast_Score=330, Evalue=3e-91,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=47.6377952755905, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24654573, Length=437, Percent_Identity=49.6567505720824, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655555, Length=511, Percent_Identity=40.5088062622309, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655560, Length=511, Percent_Identity=40.5088062622309, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655570, Length=511, Percent_Identity=40.5088062622309, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655574, Length=511, Percent_Identity=40.5088062622309, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655565, Length=511, Percent_Identity=40.5088062622309, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19922058, Length=548, Percent_Identity=35.036496350365, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24652871, Length=504, Percent_Identity=35.1190476190476, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI21357867, Length=536, Percent_Identity=29.2910447761194, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24656367, Length=517, Percent_Identity=29.4003868471954, Blast_Score=205, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24656372, Length=377, Percent_Identity=30.2387267904509, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45552909, Length=334, Percent_Identity=29.0419161676647, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54659; Mature: 54528

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFAEFIAAIDQGTTSTRCVIFDHNGAEVARHQLEHEQILPRSGWVEHNPVEIWERTAS
CCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VLMSVLNATNLSAKDIAALGITNQRETTLVWNRNTGRPYCNAIVWQDTRTDRIAVALDRD
HHHHHHHCCCCCHHHEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEECCC
GRGEVIRRKAGLPPATYFSGGKLQWILENVDGVGAAAENGEALFGTPDTWLLWNLTGGPR
CCCHHHHHHCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEEEECCCCC
GGVHVTDVTNASRTMLMDLEKLDWDDELLSFFSIPRAMLPAIASSSPLQPYGVTLADGPV
CCEEEEECCCCCHHEEEEHHHCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCEEEEECCCC
GGEVPITGVLGDQHAAMVGQVCLDAGEAKNTYGTGNFLLLNTGEAIVRSGNGLLTTVCYQ
CCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHEECCCCEEEEHHHH
FGDAKPVYALEGSIAVTGSAVQWLRDQLGIISGAAQSESLARQVVDNGGVYFVPAFSGLF
CCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECHHHHC
SPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVVDAMAADSGVRLDVLKVDGGI
CHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCC
TSNDLCMQIQADVLGVDVVRSVVAETTALGSAYAAGLAVGFWAGPSDLRANWQEDKRWTP
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCCCCCCCCC
TWGEDQRAAAYAGWRKAVQRTLDWVDVS
CCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEFAEFIAAIDQGTTSTRCVIFDHNGAEVARHQLEHEQILPRSGWVEHNPVEIWERTAS
CHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VLMSVLNATNLSAKDIAALGITNQRETTLVWNRNTGRPYCNAIVWQDTRTDRIAVALDRD
HHHHHHHCCCCCHHHEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEECCC
GRGEVIRRKAGLPPATYFSGGKLQWILENVDGVGAAAENGEALFGTPDTWLLWNLTGGPR
CCCHHHHHHCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEEEECCCCC
GGVHVTDVTNASRTMLMDLEKLDWDDELLSFFSIPRAMLPAIASSSPLQPYGVTLADGPV
CCEEEEECCCCCHHEEEEHHHCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCEEEEECCCC
GGEVPITGVLGDQHAAMVGQVCLDAGEAKNTYGTGNFLLLNTGEAIVRSGNGLLTTVCYQ
CCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHEECCCCEEEEHHHH
FGDAKPVYALEGSIAVTGSAVQWLRDQLGIISGAAQSESLARQVVDNGGVYFVPAFSGLF
CCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECHHHHC
SPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVVDAMAADSGVRLDVLKVDGGI
CHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCC
TSNDLCMQIQADVLGVDVVRSVVAETTALGSAYAAGLAVGFWAGPSDLRANWQEDKRWTP
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCCCCCCCCC
TWGEDQRAAAYAGWRKAVQRTLDWVDVS
CCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA