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The map label for this gene is yfhM [H]

Identifier: 221230706

GI number: 221230706

Start: 2721935

End: 2722909

Strand: Direct

Name: yfhM [H]

Synonym: MLBr_02297

Alternate gene names: 221230706

Gene position: 2721935-2722909 (Clockwise)

Preceding gene: 221230705

Following gene: 221230711

Centisome position: 83.29

GC content: 61.85

Gene sequence:

>975_bases
ATGGCAATGTCGAACCCGTCGGTGACCCGTATCGCCGGGCCATGGCACCATCTGGACGTGCACGCCAACGGCATCCGATT
CCACGTCGTCGAGGCGGTGCCCACCCAGCCAGCCGGCCAGGACTGCGCCCCTTCGGCTACGGCACGACCACTGGTAATGC
TGCTGCATGGTTTCGGTTCTTTCTGGTGGTCCTGGCGTCATCAGCTACGCGGCCTGACCGAAGCGCGACTGGTCGCGGTG
GACCTGCGTGGCTACGGTGGCAGCGACAAACCGCCCCGCGGCTATGACGGCTGGACACTTGCCGGCGACACAGCCGGGCT
CATCCGAGCACTGGGGCACTCATCGGCGACACTGGTTGGCCACGCCGAAGGCGGGCTTGCGTGCTGGGCCACCGCGCTGC
TGCACCCACGGCTGGTGCGCGCCATTGCCCTGATCAACTCACCGCACCCAGCAGCGTTACGACGATCAATGCTGACGCAC
CGTAATCAAGGTTACGCACTGCTGCCGACGTTGCTACGCTACCAGTTACCAATTTGGCCGGAACGTTTGCTGACCCGCAA
TAACGCAGCGGAGATCGAACGCCTAGTCCGCAGTCGCAGCAGTGCCAAATGGCAAGCTTGCGAGGATTTCGCGGTCACGA
TCGACCATTTGCGTATAGCGATCCAGATTCCGGCTGCCGCGCACTGTGCACTCGAGTATCAGCGTTGGGCAGTGCGTAGC
CAGCTACGTAACGAAGGCCGTAAATTCATGAAATCGATGTCTCGACCATTCAGCGTACCGCTGCTGCACGTACGCGGCGA
CGCGGATCCCTACCTGCTAGCCGACTCGGTGGACCGAACACGGCGCTATGCACCGCACGGACGCTACGTATCCGTTTCCG
ACGCAGGACATTTCAGTCACGAAGAAGCACCAGAGGAAATTAACAGATACCTGACGCGGTTTCTCAAGCAGGTTCACGGG
CCCCGGCTCAGCTGA

Upstream 100 bases:

>100_bases
CGATAAGGCGCAAGCCAGGCCGCGCAAACTCACCGGATCCGGCACTAACCCGGAAAACAATCCCGACCGCAGGACCCCCG
CCGATCCCTCGGGTTGGTAG

Downstream 100 bases:

>100_bases
CGCAGGCCCCAGTGGCCACCGCCTCCTGGGTCTCACCGATTTCGGTAAGCCGGTGGGACACTTCCTCAGCCGTCAACACG
AAACCAGTTTCGGAATCATC

Product: putative hydrolase

Products: glycol

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAMSNPSVTRIAGPWHHLDVHANGIRFHVVEAVPTQPAGQDCAPSATARPLVMLLHGFGSFWWSWRHQLRGLTEARLVAV
DLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHSSATLVGHAEGGLACWATALLHPRLVRAIALINSPHPAALRRSMLTH
RNQGYALLPTLLRYQLPIWPERLLTRNNAAEIERLVRSRSSAKWQACEDFAVTIDHLRIAIQIPAAAHCALEYQRWAVRS
QLRNEGRKFMKSMSRPFSVPLLHVRGDADPYLLADSVDRTRRYAPHGRYVSVSDAGHFSHEEAPEEINRYLTRFLKQVHG
PRLS

Sequences:

>Translated_324_residues
MAMSNPSVTRIAGPWHHLDVHANGIRFHVVEAVPTQPAGQDCAPSATARPLVMLLHGFGSFWWSWRHQLRGLTEARLVAV
DLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHSSATLVGHAEGGLACWATALLHPRLVRAIALINSPHPAALRRSMLTH
RNQGYALLPTLLRYQLPIWPERLLTRNNAAEIERLVRSRSSAKWQACEDFAVTIDHLRIAIQIPAAAHCALEYQRWAVRS
QLRNEGRKFMKSMSRPFSVPLLHVRGDADPYLLADSVDRTRRYAPHGRYVSVSDAGHFSHEEAPEEINRYLTRFLKQVHG
PRLS
>Mature_323_residues
AMSNPSVTRIAGPWHHLDVHANGIRFHVVEAVPTQPAGQDCAPSATARPLVMLLHGFGSFWWSWRHQLRGLTEARLVAVD
LRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHSSATLVGHAEGGLACWATALLHPRLVRAIALINSPHPAALRRSMLTHR
NQGYALLPTLLRYQLPIWPERLLTRNNAAEIERLVRSRSSAKWQACEDFAVTIDHLRIAIQIPAAAHCALEYQRWAVRSQ
LRNEGRKFMKSMSRPFSVPLLHVRGDADPYLLADSVDRTRRYAPHGRYVSVSDAGHFSHEEAPEEINRYLTRFLKQVHGP
RLS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=326, Percent_Identity=28.5276073619632, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI27597073, Length=284, Percent_Identity=27.112676056338, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI218777840, Length=324, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI13376164, Length=324, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554012, Length=307, Percent_Identity=25.0814332247557, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17562298, Length=311, Percent_Identity=26.3665594855305, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.3.2.3

Molecular weight: Translated: 36212; Mature: 36081

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSNPSVTRIAGPWHHLDVHANGIRFHVVEAVPTQPAGQDCAPSATARPLVMLLHGFGS
CCCCCCCCEEEECCCEEEEEEECCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
FWWSWRHQLRGLTEARLVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHSSATLVG
HHHHHHHHHCCCCHHEEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEEE
HAEGGLACWATALLHPRLVRAIALINSPHPAALRRSMLTHRNQGYALLPTLLRYQLPIWP
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCH
ERLLTRNNAAEIERLVRSRSSAKWQACEDFAVTIDHLRIAIQIPAAAHCALEYQRWAVRS
HHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHH
QLRNEGRKFMKSMSRPFSVPLLHVRGDADPYLLADSVDRTRRYAPHGRYVSVSDAGHFSH
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHHHHHHCCCCCCEEEECCCCCCCC
EEAPEEINRYLTRFLKQVHGPRLS
CCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AMSNPSVTRIAGPWHHLDVHANGIRFHVVEAVPTQPAGQDCAPSATARPLVMLLHGFGS
CCCCCCCEEEECCCEEEEEEECCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
FWWSWRHQLRGLTEARLVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHSSATLVG
HHHHHHHHHCCCCHHEEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEEE
HAEGGLACWATALLHPRLVRAIALINSPHPAALRRSMLTHRNQGYALLPTLLRYQLPIWP
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCH
ERLLTRNNAAEIERLVRSRSSAKWQACEDFAVTIDHLRIAIQIPAAAHCALEYQRWAVRS
HHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHH
QLRNEGRKFMKSMSRPFSVPLLHVRGDADPYLLADSVDRTRRYAPHGRYVSVSDAGHFSH
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHHHHHHCCCCCCEEEECCCCCCCC
EEAPEEINRYLTRFLKQVHGPRLS
CCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; epoxide

Specific reaction: H2O + an epoxide = a glycol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]