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The map label for this gene is menE [H]

Identifier: 221230682

GI number: 221230682

Start: 2681302

End: 2682408

Strand: Reverse

Name: menE [H]

Synonym: MLBr_02257

Alternate gene names: 221230682

Gene position: 2682408-2681302 (Counterclockwise)

Preceding gene: 221230683

Following gene: 221230680

Centisome position: 82.08

GC content: 63.78

Gene sequence:

>1107_bases
TTGTTGCTGCCCACGCTGGAACGAATCCTGGATGGCCGCGCTCCCGCAATGGTCGTGGTACCGGCTCAGCATGAACCCAT
CGCTGCGCTGCGTGCTTTGCGAGTAGGTGAAGAGATCGACGACGATGTGGCTTTAGTGGCCACCACGTCGGGAACGACAG
GTGTGCCCAAGGGCGCCTTGCTGACTGCCGCCGCCTTGACTGCCAGCGCGTCGGCCACCCACGACCGCCTTGGTGGTCCG
GGCAGCTGGCTGCTGGCTTTGCCGCCGTATCATATCGGCGGGCTTCAGGTGCTGGTGCGTAGCGTGCTGGCCGGTTCGGT
ACCCATCGAGTTGGACATTTCTGCCGGGTTTGACCTCGCCAAACTGCCGGACGCGGTGGGTCGATTGGGTACCGGTCGAC
GATACACGTCACTGGTCGCTACCCAGCTGGCCAAGGCGCTCACCGACTCAACGGCCACGGCCGTGCTAGCCGAATTGGAT
GCCGTCCTCGTTGGTGGCGGACCAGCTCCCCGGCCGATCCTGGATGCCGCAACATCTGCCGGCATCATGGTGGTCGGCAC
CTATGGGATGAGCGAGACTGCGGGAGGTTGCGTTTACGACGGCGTCCCGCTGGATGGTGTGTGCATACGCGTACTGGTCA
ATGGCCACGTAGCGATCGGTGGTGCGACCTTGGCCAAGGGCTATCGCAATCCGATCGTTCCCGACCCGTTCGCCGAATCG
GGTTGGTTCCACACAGACGACCTCGGTACTGTCGATGGCTCGGGTGTGCTAACAGTGCTGGGTCGGGCCGACGACGCGAT
CAGCACGGGTGGGCTAACCGTGCTGCCAGGACCCGTCGAAGCTGCGCTGTGCACCCATCCCGCGGTCAGCGACTGTGCGG
TGTTCGGGCTTACTGACGACCGGCTAGGTCAGCGGGTAGTAGCCGCAGTGGTACTGACTGACGGTTACGCAACGCCGACG
CTGAGCATGTTGCGGGCACATGTCACCCGCAGACTGGACGCCATGGCCGCACCGCGCGAGCTACACATCGTTGATACACT
GCCACGACGTGGCATCGGAAAGGTCGATCGCACGGCGTTAGTGCGCCGGTTCGCCAAGTCCGGTTAA

Upstream 100 bases:

>100_bases
TGAACGCCAAGGGCTGGCTGCTCGATAACGCACGGGACAACGGAGAACCCACATAGCCGTCCTTCGCTCGCTTAGCGTTA
CGTCGGGTTCCGCAGCTCCA

Downstream 100 bases:

>100_bases
TTAGGCTGACCGATCGTGAAGATAGGTCGCCGAAAGACATTGTCCGTCGCAGCAGCGGTTGAACTGCGGTATCCATCCGT
GGCGAGACACCCGGCTAGAA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MLLPTLERILDGRAPAMVVVPAQHEPIAALRALRVGEEIDDDVALVATTSGTTGVPKGALLTAAALTASASATHDRLGGP
GSWLLALPPYHIGGLQVLVRSVLAGSVPIELDISAGFDLAKLPDAVGRLGTGRRYTSLVATQLAKALTDSTATAVLAELD
AVLVGGGPAPRPILDAATSAGIMVVGTYGMSETAGGCVYDGVPLDGVCIRVLVNGHVAIGGATLAKGYRNPIVPDPFAES
GWFHTDDLGTVDGSGVLTVLGRADDAISTGGLTVLPGPVEAALCTHPAVSDCAVFGLTDDRLGQRVVAAVVLTDGYATPT
LSMLRAHVTRRLDAMAAPRELHIVDTLPRRGIGKVDRTALVRRFAKSG

Sequences:

>Translated_368_residues
MLLPTLERILDGRAPAMVVVPAQHEPIAALRALRVGEEIDDDVALVATTSGTTGVPKGALLTAAALTASASATHDRLGGP
GSWLLALPPYHIGGLQVLVRSVLAGSVPIELDISAGFDLAKLPDAVGRLGTGRRYTSLVATQLAKALTDSTATAVLAELD
AVLVGGGPAPRPILDAATSAGIMVVGTYGMSETAGGCVYDGVPLDGVCIRVLVNGHVAIGGATLAKGYRNPIVPDPFAES
GWFHTDDLGTVDGSGVLTVLGRADDAISTGGLTVLPGPVEAALCTHPAVSDCAVFGLTDDRLGQRVVAAVVLTDGYATPT
LSMLRAHVTRRLDAMAAPRELHIVDTLPRRGIGKVDRTALVRRFAKSG
>Mature_368_residues
MLLPTLERILDGRAPAMVVVPAQHEPIAALRALRVGEEIDDDVALVATTSGTTGVPKGALLTAAALTASASATHDRLGGP
GSWLLALPPYHIGGLQVLVRSVLAGSVPIELDISAGFDLAKLPDAVGRLGTGRRYTSLVATQLAKALTDSTATAVLAELD
AVLVGGGPAPRPILDAATSAGIMVVGTYGMSETAGGCVYDGVPLDGVCIRVLVNGHVAIGGATLAKGYRNPIVPDPFAES
GWFHTDDLGTVDGSGVLTVLGRADDAISTGGLTVLPGPVEAALCTHPAVSDCAVFGLTDDRLGQRVVAAVVLTDGYATPT
LSMLRAHVTRRLDAMAAPRELHIVDTLPRRGIGKVDRTALVRRFAKSG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=388, Percent_Identity=26.8041237113402, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI187761343, Length=388, Percent_Identity=26.8041237113402, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI156151445, Length=360, Percent_Identity=23.6111111111111, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI115511026, Length=125, Percent_Identity=33.6, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI122937307, Length=120, Percent_Identity=36.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI13375727, Length=123, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788595, Length=324, Percent_Identity=31.7901234567901, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693145, Length=361, Percent_Identity=27.9778393351801, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788107, Length=388, Percent_Identity=26.5463917525773, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=350, Percent_Identity=28.8571428571429, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=358, Percent_Identity=28.7709497206704, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=361, Percent_Identity=26.0387811634349, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=373, Percent_Identity=24.3967828418231, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=366, Percent_Identity=24.0437158469945, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=386, Percent_Identity=23.5751295336788, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556356, Length=235, Percent_Identity=26.3829787234043, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=372, Percent_Identity=25, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=134, Percent_Identity=34.3283582089552, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581924, Length=356, Percent_Identity=24.7191011235955, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356947, Length=354, Percent_Identity=27.4011299435028, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24653035, Length=368, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 37626; Mature: 37626

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPTLERILDGRAPAMVVVPAQHEPIAALRALRVGEEIDDDVALVATTSGTTGVPKGAL
CCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHH
LTAAALTASASATHDRLGGPGSWLLALPPYHIGGLQVLVRSVLAGSVPIELDISAGFDLA
HHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHH
KLPDAVGRLGTGRRYTSLVATQLAKALTDSTATAVLAELDAVLVGGGPAPRPILDAATSA
HCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHCCC
GIMVVGTYGMSETAGGCVYDGVPLDGVCIRVLVNGHVAIGGATLAKGYRNPIVPDPFAES
CEEEEEECCCCCCCCCEEECCCCCCCEEEEEEECCCEEECCHHHHHCCCCCCCCCCCCCC
GWFHTDDLGTVDGSGVLTVLGRADDAISTGGLTVLPGPVEAALCTHPAVSDCAVFGLTDD
CCEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHCCCCCCCEEEECCCHH
RLGQRVVAAVVLTDGYATPTLSMLRAHVTRRLDAMAAPRELHIVDTLPRRGIGKVDRTAL
HHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
VRRFAKSG
HHHHHHCC
>Mature Secondary Structure
MLLPTLERILDGRAPAMVVVPAQHEPIAALRALRVGEEIDDDVALVATTSGTTGVPKGAL
CCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHH
LTAAALTASASATHDRLGGPGSWLLALPPYHIGGLQVLVRSVLAGSVPIELDISAGFDLA
HHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHH
KLPDAVGRLGTGRRYTSLVATQLAKALTDSTATAVLAELDAVLVGGGPAPRPILDAATSA
HCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHCCC
GIMVVGTYGMSETAGGCVYDGVPLDGVCIRVLVNGHVAIGGATLAKGYRNPIVPDPFAES
CEEEEEECCCCCCCCCEEECCCCCCCEEEEEEECCCEEECCHHHHHCCCCCCCCCCCCCC
GWFHTDDLGTVDGSGVLTVLGRADDAISTGGLTVLPGPVEAALCTHPAVSDCAVFGLTDD
CCEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHCCCCCCCEEEECCCHH
RLGQRVVAAVVLTDGYATPTLSMLRAHVTRRLDAMAAPRELHIVDTLPRRGIGKVDRTAL
HHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
VRRFAKSG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]