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The map label for this gene is otsA

Identifier: 221230681

GI number: 221230681

Start: 2677188

End: 2678684

Strand: Direct

Name: otsA

Synonym: MLBr_02254

Alternate gene names: 221230681

Gene position: 2677188-2678684 (Clockwise)

Preceding gene: 221230677

Following gene: 221230690

Centisome position: 81.92

GC content: 60.39

Gene sequence:

>1497_bases
ATGACTTCGCGGGGTAACCATGGCTCGAAGACCTCAAGTGACAAGCACCTCGGGGATTCCGACTTTGTAGTGGTCGCCAA
TCGCCTGCCAGTTGATCAGGTGCGACTTCCCGATGGCACTGCGATCTGGAAGCGCAGTCCAGGCGGGTTGGTCACAGCGC
TAGAGCCGCTGCTGCGTCAGCGGCGCGGGGCCTGGGTCGGCTGGCCGGGCGTAATAAACGACAACGTGGACCTCGACTTG
ACCATCAAGTCGATCGTGCAGGATGGCCTTACTCTTTATCCGGTACGGCTGAACACACATGATGTCGCCGAGTACTACGA
GGGGTTCTCGAACGCCACGCTGTGGCCGCTATACCACGACGTCATCGTAAAGCCCATTTACCACTGCGAATGGTGGGAAC
GCTACGTCGATGTCAACCGGCGCTTCGCCGAAACAACGTCACGCACAGCCGCCTACGGTGGGACCGTATGGGTGCAGGAC
TACCAACTGCAATTGGTACCGAAGATGCTGCGCATAATGCGGCCCGACTTGACCATCGGTTTCTTCTTGCACATCCCGTT
CCCGCCGGTGGAGCTGTTCATGCAGATACCATGGCGCACCGAGATCATCGAGGGTCTGCTCGGGGCCGACCTAGTGGGAT
TCCATCTGACCAGCGGTGCGCAGAATTTCCTGTTCCTGTCCCGACATCTGCTCGGCGCTAACACGTCGCGCGGATTGGTT
GGTGTGCGATCACGGTTCGGTGAAGTGCAATTGAAGTCCCACACAGTCCAAGTGGGCGCGTTTCCCATCTCGATCGACTC
CAAAGAAATCGACCAAGCCACCCGCGACCGCAACGTCCGGCGCCGCGCCCGCGAAATCCGGGCTGAGCTAGGTAACCCCC
GCAAAATCCTGCTCGGCGTTGACCGACTCGACTACACCAAGGGCATCGATGTTCGATTGAGGGCCTTCGCTGAACTGCTC
GCCGAGGGTCGGGCGAAGCGCGACGACACCGTTCTAGTCCAGCTAGCGACACCGAGCCGCGAACGAGTAGAAAGCTATAA
GATTTTACGCAACGACATCGAACGCCAAGTTGGCCATATCAACGGCGAATACGGCGAGGTCGGTCATCCCGTGGTGCACT
ACTTGCACCGCCCAATTCCCCGCGACGAGCTCATCGCGTTCTACGTGGCAAGTGATGTCATGTTGGTCACCCCGCTGCGG
GACGGGATGAACCTAGTGGCCAAGGAATACGTCGCGTGCCGCAACGATCTCGGCGGTGCGCTCGTCCTCAGCGAATTCAC
CGGAGCAGCCGCGGAACTCCGGCAGGCCTATCTAGTCAACCCACACGACCTAGAAGGGGTTAAGGACACGATCGAAGCGG
CGCTCAACCAGCTGGCCGAAGAAGCACGGCGCCGGATGCGGTCGCTGCGACGCCAAGTGCTCGCCCATGACGTCGATCGC
TGGGCGCGATCATTTCTCGACGCGCTCGCCGAAGCGCCGGCGCGCGACGCGACATAA

Upstream 100 bases:

>100_bases
CCAGGCCAGGCGCGTCGTCGCCGCGTATGCCAACGACGCGGACGAGTGCCGGATTTTCCTATCTATGCTCGGCATTGGGC
CAGCGAAACACGAGACGTAA

Downstream 100 bases:

>100_bases
CTTGCATCTCTATGTCACTTCCCACCGTGGTGTTGTCGCCGAAACGCTATGGATACTCAATTGCAGCGCGAACAGAGGCG
CGCCGTGCCATACATCCGTC

Product: putative trehalose-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTSRGNHGSKTSSDKHLGDSDFVVVANRLPVDQVRLPDGTAIWKRSPGGLVTALEPLLRQRRGAWVGWPGVINDNVDLDL
TIKSIVQDGLTLYPVRLNTHDVAEYYEGFSNATLWPLYHDVIVKPIYHCEWWERYVDVNRRFAETTSRTAAYGGTVWVQD
YQLQLVPKMLRIMRPDLTIGFFLHIPFPPVELFMQIPWRTEIIEGLLGADLVGFHLTSGAQNFLFLSRHLLGANTSRGLV
GVRSRFGEVQLKSHTVQVGAFPISIDSKEIDQATRDRNVRRRAREIRAELGNPRKILLGVDRLDYTKGIDVRLRAFAELL
AEGRAKRDDTVLVQLATPSRERVESYKILRNDIERQVGHINGEYGEVGHPVVHYLHRPIPRDELIAFYVASDVMLVTPLR
DGMNLVAKEYVACRNDLGGALVLSEFTGAAAELRQAYLVNPHDLEGVKDTIEAALNQLAEEARRRMRSLRRQVLAHDVDR
WARSFLDALAEAPARDAT

Sequences:

>Translated_498_residues
MTSRGNHGSKTSSDKHLGDSDFVVVANRLPVDQVRLPDGTAIWKRSPGGLVTALEPLLRQRRGAWVGWPGVINDNVDLDL
TIKSIVQDGLTLYPVRLNTHDVAEYYEGFSNATLWPLYHDVIVKPIYHCEWWERYVDVNRRFAETTSRTAAYGGTVWVQD
YQLQLVPKMLRIMRPDLTIGFFLHIPFPPVELFMQIPWRTEIIEGLLGADLVGFHLTSGAQNFLFLSRHLLGANTSRGLV
GVRSRFGEVQLKSHTVQVGAFPISIDSKEIDQATRDRNVRRRAREIRAELGNPRKILLGVDRLDYTKGIDVRLRAFAELL
AEGRAKRDDTVLVQLATPSRERVESYKILRNDIERQVGHINGEYGEVGHPVVHYLHRPIPRDELIAFYVASDVMLVTPLR
DGMNLVAKEYVACRNDLGGALVLSEFTGAAAELRQAYLVNPHDLEGVKDTIEAALNQLAEEARRRMRSLRRQVLAHDVDR
WARSFLDALAEAPARDAT
>Mature_497_residues
TSRGNHGSKTSSDKHLGDSDFVVVANRLPVDQVRLPDGTAIWKRSPGGLVTALEPLLRQRRGAWVGWPGVINDNVDLDLT
IKSIVQDGLTLYPVRLNTHDVAEYYEGFSNATLWPLYHDVIVKPIYHCEWWERYVDVNRRFAETTSRTAAYGGTVWVQDY
QLQLVPKMLRIMRPDLTIGFFLHIPFPPVELFMQIPWRTEIIEGLLGADLVGFHLTSGAQNFLFLSRHLLGANTSRGLVG
VRSRFGEVQLKSHTVQVGAFPISIDSKEIDQATRDRNVRRRAREIRAELGNPRKILLGVDRLDYTKGIDVRLRAFAELLA
EGRAKRDDTVLVQLATPSRERVESYKILRNDIERQVGHINGEYGEVGHPVVHYLHRPIPRDELIAFYVASDVMLVTPLRD
GMNLVAKEYVACRNDLGGALVLSEFTGAAAELRQAYLVNPHDLEGVKDTIEAALNQLAEEARRRMRSLRRQVLAHDVDRW
ARSFLDALAEAPARDAT

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family

Homologues:

Organism=Escherichia coli, GI1788206, Length=484, Percent_Identity=34.297520661157, Blast_Score=237, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI115533558, Length=545, Percent_Identity=26.7889908256881, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI115533560, Length=545, Percent_Identity=26.7889908256881, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71987012, Length=430, Percent_Identity=29.0697674418605, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=495, Percent_Identity=36.7676767676768, Blast_Score=312, Evalue=9e-86,
Organism=Saccharomyces cerevisiae, GI6323537, Length=473, Percent_Identity=29.3868921775898, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320279, Length=450, Percent_Identity=30.4444444444444, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323917, Length=436, Percent_Identity=28.6697247706422, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI19920676, Length=496, Percent_Identity=35.2822580645161, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSA_MYCLE (Q50167)

Other databases:

- EMBL:   U15187
- EMBL:   AL583924
- PIR:   B87191
- RefSeq:   NP_302467.1
- HSSP:   P31677
- ProteinModelPortal:   Q50167
- SMR:   Q50167
- EnsemblBacteria:   EBMYCT00000028875
- GeneID:   908776
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2254
- NMPDR:   fig|272631.1.peg.1339
- Leproma:   ML2254
- GeneTree:   EBGT00050000016525
- HOGENOM:   HBG559076
- OMA:   CHYRLDL
- ProtClustDB:   CLSK872185
- BioCyc:   MLEP272631:ML2254-MONOMER
- InterPro:   IPR001830

Pfam domain/function: PF00982 Glyco_transf_20

EC number: 2.4.1.15

Molecular weight: Translated: 56301; Mature: 56170

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 106-106 BINDING 160-160 BINDING 302-302 BINDING 307-307 BINDING 340-340

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRGNHGSKTSSDKHLGDSDFVVVANRLPVDQVRLPDGTAIWKRSPGGLVTALEPLLRQ
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHH
RRGAWVGWPGVINDNVDLDLTIKSIVQDGLTLYPVRLNTHDVAEYYEGFSNATLWPLYHD
HCCCEECCCCCCCCCCCEEEEHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCEEHHHHHH
VIVKPIYHCEWWERYVDVNRRFAETTSRTAAYGGTVWVQDYQLQLVPKMLRIMRPDLTIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCEEE
FFLHIPFPPVELFMQIPWRTEIIEGLLGADLVGFHLTSGAQNFLFLSRHLLGANTSRGLV
EEEECCCCHHHHHEECCCHHHHHHHHHCCHHHHHEECCCCHHHHHHHHHHHCCCCCCCHH
GVRSRFGEVQLKSHTVQVGAFPISIDSKEIDQATRDRNVRRRAREIRAELGNPRKILLGV
HHHHHCCCEEEECEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
DRLDYTKGIDVRLRAFAELLAEGRAKRDDTVLVQLATPSRERVESYKILRNDIERQVGHI
CHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
NGEYGEVGHPVVHYLHRPIPRDELIAFYVASDVMLVTPLRDGMNLVAKEYVACRNDLGGA
CCCCCCCCCHHHHHHHCCCCHHHHEEEEEECCEEEEEECHHHHHHHHHHHHHHHCCCCCE
LVLSEFTGAAAELRQAYLVNPHDLEGVKDTIEAALNQLAEEARRRMRSLRRQVLAHDVDR
EEEHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WARSFLDALAEAPARDAT
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSRGNHGSKTSSDKHLGDSDFVVVANRLPVDQVRLPDGTAIWKRSPGGLVTALEPLLRQ
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHH
RRGAWVGWPGVINDNVDLDLTIKSIVQDGLTLYPVRLNTHDVAEYYEGFSNATLWPLYHD
HCCCEECCCCCCCCCCCEEEEHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCEEHHHHHH
VIVKPIYHCEWWERYVDVNRRFAETTSRTAAYGGTVWVQDYQLQLVPKMLRIMRPDLTIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCEEE
FFLHIPFPPVELFMQIPWRTEIIEGLLGADLVGFHLTSGAQNFLFLSRHLLGANTSRGLV
EEEECCCCHHHHHEECCCHHHHHHHHHCCHHHHHEECCCCHHHHHHHHHHHCCCCCCCHH
GVRSRFGEVQLKSHTVQVGAFPISIDSKEIDQATRDRNVRRRAREIRAELGNPRKILLGV
HHHHHCCCEEEECEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
DRLDYTKGIDVRLRAFAELLAEGRAKRDDTVLVQLATPSRERVESYKILRNDIERQVGHI
CHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
NGEYGEVGHPVVHYLHRPIPRDELIAFYVASDVMLVTPLRDGMNLVAKEYVACRNDLGGA
CCCCCCCCCHHHHHHHCCCCHHHHEEEEEECCEEEEEECHHHHHHHHHHHHHHHCCCCCE
LVLSEFTGAAAELRQAYLVNPHDLEGVKDTIEAALNQLAEEARRRMRSLRRQVLAHDVDR
EEEHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WARSFLDALAEAPARDAT
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002