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The map label for this gene is fadA [H]

Identifier: 221230637

GI number: 221230637

Start: 2571232

End: 2572443

Strand: Reverse

Name: fadA [H]

Synonym: MLBr_02162

Alternate gene names: 221230637

Gene position: 2572443-2571232 (Counterclockwise)

Preceding gene: 221230639

Following gene: 221230636

Centisome position: 78.71

GC content: 63.12

Gene sequence:

>1212_bases
ATGTCCGAAGAAGCTTTCATCTATGAGGCCATCCGCACCCCTCGCGGTAAGCAGAAGAACGGATCGTTGCACGAGGTCAA
GCCGGTAAGCCTGGTCGTTGGGCTAGTCGAGGAATTGCGTAAGCGAAACCCCGATCTCGATGAGAACCTGATCAGCGACG
TCATCTTGGGATGCGTCTCACCGGTGGGCGATCAGGGCGGTGACATTGCCCGCGCCACGGTGCTGGCCGCTGGCCTGCCT
GTCACCGCAGGCGGGGTGCAGCTCAACCGGTTCTGCGCATCGGGGCTTGAAGCCGTTAACACCGCCGCGCAGAAGGTGCG
CTCTGGCTGGGATGACCTGGTTCTGGCTGGCGGTGTGGAGTCGATGAGCCGGGTGCCGATGGGATCCGATGGCGGCGCGA
TGGGCTTGGACCCGGCGACTAACTACGACGTCATGTTCGTCCCGCAGGGCATTGGTGCCGACCTGATCGCCACCATCGAG
GGCTTCTCGCGTGATGACGTCGACGTCTATGCGTTGCGCAGCCAGGAGAAGGCCGCGGCCGCGTGGTCGGGCGGCTACTT
CGCCAAGTCGGTGGTTCCGGTGCGCGACCAGAATGGTCTGTTGATCCTCGATCACGACGAGCACATGCGGCCCGACACCT
CCAAGGAGGGCCTGGCCAAGCTGAAGCCCGCCTTCGAGGGTCTTGCCGCGATGGGTGGTTTCGACGACGTGGCCCTGCAG
AAATATCACTGGGTGGAGAAGATCAACCACGTTCACACCGGAGGTAACAGCTCCGGCATCGTCGACGGCGCGGCACTGGT
GATGATTGGCAGCGAAGCTGTTGGCAAGTCCCAGGGCCTGACTCCGCGGGCGCGCATGGTAGCCACCGCTACCAGTGGGG
CCGACCCGGTCATCATGCTGACCGGCCCCACCCCCGCCACCCGCAAGGTGCTCGACCGCGCCGGGTTGACTGTTGATGAC
ATCGACCTGTTCGAGTTAAACGAGGCGTTTGCGTCGGTGGTTCTGAAATTCCAGAAAGACCTGAACATTCCGGACGAGAA
GCTCAACGTCAACGGCGGTGCTATCGCGATGGGTCACCCGCTGGGTGCCACCGGCGCGATGATCCTCGGGACCATGGTCG
ACGAACTGGAGCGCTGCAACGCGCGGCGTGCGCTCGTCACGCTATGTATCGGCGCCGGCATGGGTGTTGCCACCATCATC
GAGAGGGTTTAA

Upstream 100 bases:

>100_bases
TGTTAGTGTGCCTGTACTGTACTAGTTCCTAGTCTTTAAGACGGAATCTAACCGCCCACATCAATAGTAAATTAAATTTT
TTTGCGAACAGGAAGCCGAC

Downstream 100 bases:

>100_bases
TAGCATGGCAGACACTGCAATTCAGTGGAGTAAGGACGCCGACGGTATTGTCACGCTGACAATGAACGACCCGTCCAGGT
CGGCCAACGTTATGAACGAG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEEAFIYEAIRTPRGKQKNGSLHEVKPVSLVVGLVEELRKRNPDLDENLISDVILGCVSPVGDQGGDIARATVLAAGLP
VTAGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQGIGADLIATIE
GFSRDDVDVYALRSQEKAAAAWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTSKEGLAKLKPAFEGLAAMGGFDDVALQ
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSEAVGKSQGLTPRARMVATATSGADPVIMLTGPTPATRKVLDRAGLTVDD
IDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERCNARRALVTLCIGAGMGVATII
ERV

Sequences:

>Translated_403_residues
MSEEAFIYEAIRTPRGKQKNGSLHEVKPVSLVVGLVEELRKRNPDLDENLISDVILGCVSPVGDQGGDIARATVLAAGLP
VTAGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQGIGADLIATIE
GFSRDDVDVYALRSQEKAAAAWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTSKEGLAKLKPAFEGLAAMGGFDDVALQ
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSEAVGKSQGLTPRARMVATATSGADPVIMLTGPTPATRKVLDRAGLTVDD
IDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERCNARRALVTLCIGAGMGVATII
ERV
>Mature_402_residues
SEEAFIYEAIRTPRGKQKNGSLHEVKPVSLVVGLVEELRKRNPDLDENLISDVILGCVSPVGDQGGDIARATVLAAGLPV
TAGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQGIGADLIATIEG
FSRDDVDVYALRSQEKAAAAWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTSKEGLAKLKPAFEGLAAMGGFDDVALQK
YHWVEKINHVHTGGNSSGIVDGAALVMIGSEAVGKSQGLTPRARMVATATSGADPVIMLTGPTPATRKVLDRAGLTVDDI
DLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERCNARRALVTLCIGAGMGVATIIE
RV

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=365, Percent_Identity=35.8904109589041, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI148539872, Length=416, Percent_Identity=35.5769230769231, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI4501853, Length=401, Percent_Identity=36.1596009975062, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI4557237, Length=419, Percent_Identity=30.7875894988067, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI4504327, Length=383, Percent_Identity=30.8093994778068, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI194353979, Length=90, Percent_Identity=52.2222222222222, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1787663, Length=423, Percent_Identity=43.2624113475177, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=38.8753056234719, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI87082165, Length=414, Percent_Identity=39.1304347826087, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1788554, Length=416, Percent_Identity=37.0192307692308, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1788683, Length=431, Percent_Identity=29.0023201856148, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI133906874, Length=376, Percent_Identity=37.7659574468085, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=415, Percent_Identity=33.4939759036145, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI25147385, Length=417, Percent_Identity=29.2565947242206, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17551802, Length=417, Percent_Identity=27.8177458033573, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=406, Percent_Identity=35.9605911330049, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6325229, Length=426, Percent_Identity=29.5774647887324, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24655093, Length=416, Percent_Identity=37.2596153846154, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17648125, Length=349, Percent_Identity=35.243553008596, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24640423, Length=367, Percent_Identity=30.2452316076294, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137578, Length=448, Percent_Identity=29.2410714285714, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42314; Mature: 42183

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEAFIYEAIRTPRGKQKNGSLHEVKPVSLVVGLVEELRKRNPDLDENLISDVILGCVS
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
PVGDQGGDIARATVLAAGLPVTAGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVE
CCCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHH
SMSRVPMGSDGGAMGLDPATNYDVMFVPQGIGADLIATIEGFSRDDVDVYALRSQEKAAA
HHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHH
AWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTSKEGLAKLKPAFEGLAAMGGFDDVALQ
HCCCCCHHHEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSEAVGKSQGLTPRARMVATATSGADPVIML
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHCCCCCCCCCHHHEEEEECCCCCCEEEE
TGPTPATRKVLDRAGLTVDDIDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHP
ECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCEEEECCC
LGATGAMILGTMVDELERCNARRALVTLCIGAGMGVATIIERV
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
SEEAFIYEAIRTPRGKQKNGSLHEVKPVSLVVGLVEELRKRNPDLDENLISDVILGCVS
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
PVGDQGGDIARATVLAAGLPVTAGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVE
CCCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHH
SMSRVPMGSDGGAMGLDPATNYDVMFVPQGIGADLIATIEGFSRDDVDVYALRSQEKAAA
HHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHH
AWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTSKEGLAKLKPAFEGLAAMGGFDDVALQ
HCCCCCHHHEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSEAVGKSQGLTPRARMVATATSGADPVIML
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHCCCCCCCCCHHHEEEEECCCCCCEEEE
TGPTPATRKVLDRAGLTVDDIDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHP
ECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCEEEECCC
LGATGAMILGTMVDELERCNARRALVTLCIGAGMGVATIIERV
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]