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The map label for this gene is gltA [H]

Identifier: 221230619

GI number: 221230619

Start: 2531539

End: 2532834

Strand: Reverse

Name: gltA [H]

Synonym: MLBr_02130

Alternate gene names: 221230619

Gene position: 2532834-2531539 (Counterclockwise)

Preceding gene: 221230620

Following gene: 221230618

Centisome position: 77.5

GC content: 56.25

Gene sequence:

>1296_bases
GTGGCCGACACCGACGACACCGTCACTCTGAAGTATCCGGGAGGCGAGATCGATCTACAGATTGTCCGTGCCACCGAAGG
TAATGACGGCATTGCGATCGTCCCGGTATTGGCCAAGACCGGTTACACTACGTTTGATGTCGGCTACGGTAACACCGCAT
CCACCAAGAGTTCCATCACCTACATTGATGGTGACGCCGGGATTCTGCGTTACCGCGGCTATCCGATTGAGCAGCTCGCC
GAGAAGTCGACCTTCATAGAGGTTTGCTATCTGCTGATCTATGGGGAGTTACCAGCCACCGATCAGCTGGCCGAATTCAC
CCATCGGATCCAGCGCCACACCATGTTGCACGAGGACCTTAAGCGATTCTATGATGGCTTCCCGCGCAACGCGCACCCGA
TGCCGGTGTTGTCCAGCGTGGTCAACGCATTGAGTGCTTACTATCAGGATGCACTGGACCCGATGGACAACGGCCAAGTA
GAGCTGTCGACGATCCGACTCTTGGCCAAGCTGCCCACTATCGCTGCCTATGCATACAAGAAGTCGGTAGGCCAGCCGTT
CCTTTACCCGGACAACGCGTTTTCTCTAGTAGAGAACTTCCTTCGAATGACTTTTGGTTTGCCGGCTGAGCCCTATGAGC
CCGACCCTGAGGTGGTCCGGGCTCTGGACATGCTGTTTATCCTGCATGCTGACCACGAGCAAAACTGTTCGACGTCAACC
GTGCGTTTGGTGGGCTCGTCGCGAGCCAACCTGTTCACCTCGATTTCGGGCGGCATTAACGCGCTGTGGGGACCGCTGCA
CGGCGGTGCCAACCAGGCGGTGCTTGAGATGCTCGAAGGTATCCGCGAGAGTGGCGACGACGTTGGTGGATTTGTCCAAA
AAGTAAAGAATTGCCAGGCCGGTGTGAAATTGATGGGCTTTGGTCACCGCGTCTACAAGAACTACGATCCGCGGGCCCGC
ATCGTCAAGGAACAGGCTGACAAGATCCTGGTCAAACTTGGTGGCGATGACGATTTGCTGAACATTGCCAAGGGACTCGA
GGAGGCGGCGCTAACCGATGACTATTTCATCGAGCGCAAGCTTTACCCGAACGTCGACTTCTACACCGGTCTGATCTACC
GGGCCCTCGGTTTTCCGACCCGGATGTTCACCGTGTTGTTTGCTTTGGGTCGGCTGCCAGGCTGGATTGCGCACTGGCGT
GAGATGCACGACGAGGGTGACAGTAAGATTGGCCGTCCGCGCCAAATATACACCGGCTACACAGAGCGTGACTACACCGC
AATTGAAACACGATAG

Upstream 100 bases:

>100_bases
AGGTTTCTAGACCCGACCCCGGGCAGGCGCTCTGTTGAGGTAACGACTACCTTCACAGCTATACCAACAGGAATATCAGC
CAGAGCGCAAAGGGGTTTTC

Downstream 100 bases:

>100_bases
TTATAGCAGTGGTGACTGCTTAGTTCATCCCGATGGTTTGCGGTCGACGACATTACCGGAAATAATCTAGACTGGCTAAC
CTCGACCATCAGGATGTTCA

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MADTDDTVTLKYPGGEIDLQIVRATEGNDGIAIVPVLAKTGYTTFDVGYGNTASTKSSITYIDGDAGILRYRGYPIEQLA
EKSTFIEVCYLLIYGELPATDQLAEFTHRIQRHTMLHEDLKRFYDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQV
ELSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNAFSLVENFLRMTFGLPAEPYEPDPEVVRALDMLFILHADHEQNCSTST
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRESGDDVGGFVQKVKNCQAGVKLMGFGHRVYKNYDPRAR
IVKEQADKILVKLGGDDDLLNIAKGLEEAALTDDYFIERKLYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWR
EMHDEGDSKIGRPRQIYTGYTERDYTAIETR

Sequences:

>Translated_431_residues
MADTDDTVTLKYPGGEIDLQIVRATEGNDGIAIVPVLAKTGYTTFDVGYGNTASTKSSITYIDGDAGILRYRGYPIEQLA
EKSTFIEVCYLLIYGELPATDQLAEFTHRIQRHTMLHEDLKRFYDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQV
ELSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNAFSLVENFLRMTFGLPAEPYEPDPEVVRALDMLFILHADHEQNCSTST
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRESGDDVGGFVQKVKNCQAGVKLMGFGHRVYKNYDPRAR
IVKEQADKILVKLGGDDDLLNIAKGLEEAALTDDYFIERKLYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWR
EMHDEGDSKIGRPRQIYTGYTERDYTAIETR
>Mature_430_residues
ADTDDTVTLKYPGGEIDLQIVRATEGNDGIAIVPVLAKTGYTTFDVGYGNTASTKSSITYIDGDAGILRYRGYPIEQLAE
KSTFIEVCYLLIYGELPATDQLAEFTHRIQRHTMLHEDLKRFYDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQVE
LSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNAFSLVENFLRMTFGLPAEPYEPDPEVVRALDMLFILHADHEQNCSTSTV
RLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRESGDDVGGFVQKVKNCQAGVKLMGFGHRVYKNYDPRARI
VKEQADKILVKLGGDDDLLNIAKGLEEAALTDDYFIERKLYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWRE
MHDEGDSKIGRPRQIYTGYTERDYTAIETR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=392, Percent_Identity=28.8265306122449, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786939, Length=427, Percent_Identity=52.4590163934426, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786527, Length=387, Percent_Identity=32.2997416020672, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=388, Percent_Identity=29.1237113402062, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324328, Length=381, Percent_Identity=28.0839895013123, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=361, Percent_Identity=26.8698060941828, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325257, Length=377, Percent_Identity=25.9946949602122, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640126, Length=389, Percent_Identity=28.2776349614396, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640124, Length=389, Percent_Identity=28.2776349614396, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356863, Length=372, Percent_Identity=27.6881720430108, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48112; Mature: 47981

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTDDTVTLKYPGGEIDLQIVRATEGNDGIAIVPVLAKTGYTTFDVGYGNTASTKSSIT
CCCCCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEHHCCCCEEEECCCCCCCCCCCCEE
YIDGDAGILRYRGYPIEQLAEKSTFIEVCYLLIYGELPATDQLAEFTHRIQRHTMLHEDL
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KRFYDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQVELSTIRLLAKLPTIAAYAYK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
KSVGQPFLYPDNAFSLVENFLRMTFGLPAEPYEPDPEVVRALDMLFILHADHEQNCSTST
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCE
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRESGDDVGGFVQKVKNCQA
EEEECCCCCHHEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GVKLMGFGHRVYKNYDPRARIVKEQADKILVKLGGDDDLLNIAKGLEEAALTDDYFIERK
CCEEEECCHHHHCCCCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHC
LYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWREMHDEGDSKIGRPRQIYTGY
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECC
TERDYTAIETR
CCCCCEEECCC
>Mature Secondary Structure 
ADTDDTVTLKYPGGEIDLQIVRATEGNDGIAIVPVLAKTGYTTFDVGYGNTASTKSSIT
CCCCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEHHCCCCEEEECCCCCCCCCCCCEE
YIDGDAGILRYRGYPIEQLAEKSTFIEVCYLLIYGELPATDQLAEFTHRIQRHTMLHEDL
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KRFYDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQVELSTIRLLAKLPTIAAYAYK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
KSVGQPFLYPDNAFSLVENFLRMTFGLPAEPYEPDPEVVRALDMLFILHADHEQNCSTST
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCE
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRESGDDVGGFVQKVKNCQA
EEEECCCCCHHEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GVKLMGFGHRVYKNYDPRARIVKEQADKILVKLGGDDDLLNIAKGLEEAALTDDYFIERK
CCEEEECCHHHHCCCCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHC
LYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWREMHDEGDSKIGRPRQIYTGY
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECC
TERDYTAIETR
CCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]